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- MakaleAçık Erişim2023Scopus SJRQ4New Approaches to Predict the Sperm Quality by the Spermatozoal miRNA Content in Fish: A Review
Kocabaş, Filiz Kutluyer; Kocabaş, Mehmet
The survival of the progeny and fertilization success depend on high-quality mature spermatozoa containing DNA and coding and non-coding RNAs besides the female gamete quality. Sperm cells and gonads in fish contain different RNA classes. It is possible that the development of fish could be affected by miRNA and/or other non-coding RNAs being abundant in Teleost fish and having important functions. Studies about the presence of miRNA in some fish species (e.g. Danio rerio, Salmo salar, Carassius auratus, Cyprinus carpio) showed that sperm motility is regulated by miRNA. Nevertheless, limited knowledge and several hypotheses have been presented about classes and content of RNAs in fish spermatozoa. More research about the effects of miRNA on fish reproduction and sperm quality are needed for population, aquatic ecosystems, and broodstock management, and the evaluation of molecular sperm quality parameters with miRNA could be used together with traditional techniques.
AtıfScopus4
İncele - MakaleAçık Erişim2022Scopus SJRQ3The Effects of Probiotic-Prebiotic on the Biomass and Protein Content of Spirulina platensis in Different Temperatures and Illuminations
Seyhaneyıldız Can, Şafak; Seyhaneyıldız, Elif; Dörücü, Mustafa
The blue-green algae Spirulina platensis Geitler 1925 has been used by people since ancient times due to its high protein content. This study was conducted to determine the effect of a Probiotic-Prebiotic (P-P) product, which contains Bifidobacterium infantis, Bifidobacterium thermophilum, Leuconostoc mesenteroides as probiotic bacteria, and beta-glucan and oligosaccharide as a prebiotic mixture on S. platensis. In the study, four groups were one of which was a control. In this study, we investigated whether the P-P product contributed to the biomass development and protein production of S. platensis under different temperatures and lighting conditions. In terms of biomass, the P-P product gave different responses at different temperatures and light intensities, but its results were positive in all groups in terms of protein ratio. The most positive effect on biomass and protein content was in the group where 0.1 ml/L P-P product was added, at 40°C and 3200 lux with 68.71% protein content and an average OD of 0.727.
AtıfScopus5WoS5OpenAlex5
İncele - MakaleAçık Erişim2019Scopus SJRQ3Study of the bioaccumulation of UO2 2+ onto the green microalgae botryococcus braunii using response surface methodology
Celik, Fatih; Aslani, Mahmoud Ali Asker; Can, Safak Seyhaneyildiz
The bioaccumulation of UO2 2+ (uranyl ion) was investigated by using dry biomass of Botryococcus braunii. Experiments were conducted based on a rotatable central composite design (CCD) and analyzed using response surface methodology (RSM), which was used to optimize the bioaccumulation process. In this goal, the effects of parameters such as initial uranium (VI) concentration, pH, temperature and contact time were studied. The optimum conditions for maximum removal (87%) of UO2 2+ from an aqueous solution were as follows: initial uranium (VI) concentration (55 mg/l), pH (3.6), temperature (46°C) and contact time (74 min). Langmuir and Freundlich isotherms and also thermodynamic parameters like Enthalpy (∆H°), Entropy (∆S°), standard Gibbs free energy (∆G°) were investigated, as well.
AtıfScopus18WoS17OpenAlex8İncele