Yazarlar
Yayınlar
- MakaleAçık Erişim2025Scopus SJRQ4Biochemical Responses of Zebra Mussel Dreissena polymorpha (Pallas, 1771) (Bivalvia: Dreissenidae) against Cadmium
Alişer, Ahmet Bedrettin; Aydin, Rahmi; Serdar, Osman
The biochemical reactions of zebra mussels Dreissena polymorpha (Pallas, 1771) exposed to different concentrations of cadmium (Cd) in the acute period (24 and 96 hours after treatment) were studied. Four variants of experimental treatment were examined: 0 μg/L Cd (control), 10 μg/L Cd, 20 μg/L Cd and 40 μg/L Cd. Changes in the enzyme activities of superoxide dismutase (SOD), catalase (CAT), glutathione peroxidase (GPx), glutathione (GSH) and thiobarbituric acid reactive substances (TBARS) levels were determined using ELISA kits. In D. polymorpha exposed to the three experimental concentrations of cadmium, there was a statistically significant decrease (p<0.05) in SOD enzyme activity with increasing concentration or exposure times. No change was observed in CAT enzyme activity with increasing concentrations. The decrease in the activity depending on the exposure times was not statistically significant (p>0.05). As for GPx enzyme activity, a significant increase was detected after Cd application compared to the control (p<0.05). TBARS and GSH levels increased significantly in all Cd application groups compared to the control at 24 and 96 hours. The effects of different concentrations of Cd on D. polymorpha in the acute period were associated with changes in SOD, CAT, GPx enzyme activities. TBARS and GSH levels were effective parameters in determining the oxidative damage caused by Cd.
- MakaleYalnız künye2023Scopus SJRQ4Individual and Combined Toxicity of Polycyclic Aromatic Hydrocarbons Phenanthrene and Fluoranthene in Freshwater Amphipod Gammarus pulex (L., 1758) (Amphipoda: Gammaridae)
Yildirim, Nuran Cikcikoglu; Serdar, Osman; Derman, Taner
The combined toxicity of polycyclic aromatic hydrocarbons on freshwater amphipod Gammarus pulex was investigated. The 14-day LC50 values of fluoranthene, phenanthrene and their combination for G. pulex were determined as 5.88±0.23, 8.31±0.57 and 7.00±0.33 mg/L, respectively. To evaluate the single and combined toxicity, sublethal concentrations of fluoranthene, phenanthrene and their combination were exposed to G. pulex for 14 days. Acetylcholinesterase, glutathione-s-transferase and cytochrome P450 1A1 enzymes were measured as biomarkers. Acetylcholinesterase activities showed significant inhibition at the end of 7 and 14 days. No statistically significant change was observed in cytochrome P450 1A1 levels at the end of the 7th day, but a significant increase was detected at the end of the 14th day. A significant inhibition was observed in glutathione-s-transferase activities at the end of the 7th and 14th days. Statistically significant changes were observed in all parameters when the application times are compared. It was determined that G. pulex exposed to different concentrations of phenanthrene and fluoranthene individually and in combination caused significant changes in activites of the all measured enzymes. It was concluded that the cytochrome P450 1A1, glutathione-s-transferase and acetylcholinesterase enzymes in G. pulex are useful biochemical markers for the determination of polycyclic aromatic hydrocarbon toxicity.
AtıfScopus3
İncele - MakaleYalnız künye2023Scopus SJRQ4Neurotoxic Effect of New Generation Insecticide with Flubendiamide Active Ingredient in Gammarus pulex (Linnaeus, 1758) (Amphipoda: Gammaridae) at Different Temperatures
Söylemez, Hilal; Serdar, Osman
This study aimed to determine the relationship between temperature and neurotoxicity of a new generation insecticide, the active ingredient of which is flubendiamide, on non-target aquatic organisms (Gammarus pulex (L., 1758)). Firstly, LC50 values of flubendiamide on G. pulex were determined at 16, 18 and 20 °C as 256.83 ± 19.59 µgL-1, 169.38 ± 34.17 µgL-1 and 155.84 ± 17.22 µgL-1, respectively. We found that the acute toxicity sensitivity of the test organism to the pesticide containing flubendiamide increased with increasing temperature. Secondly, G. pulex was exposed to non-lethal concentrations of flubendiamide pesticide for 96 hours at the above temperatures. Finally, the changes in acetylcholinesterase (AChE) enzyme activity were determined for each temperature using ELISA kits. The exposure time between the 24th and 96th h at the same temperature was found to be statistically insignificant (p>0.05). It was determined that the inhibition value of AChE enzyme activity on the test organism of the pesticide with active ingredient flubendiamide increased depending on the temperature increase. Considering the changes in AChE enzyme activities in G. pulex at different temperatures against flubendiamide insecticide, it was concluded that it can be used as a useful biomarker.
AtıfScopus1İncele - DergiActa Zoologica Bulgarica
