Sürdürülebilir Kalkınma Amaçları
Yazarlar
- Zengin, Gokhan(2)
- Acar, Mikail(1)
- Aglar, Erdal(1)
- Ak, Gunes(1)
- Akgul, Bengusu H.(1)
- Cakilcioglu, Ugur(1)
- Cvetanović Kljakić, Aleksandra(1)
- Dogan, Nurettin(1)
- Erharman, Ahmet(1)
- Erturk, Omer(1)
- Gašić, Uroš(1)
- Gulcin, Ilhami(1)
- Gun, Sefa(1)
- Koyuncu, Ismail(1)
- Li, Meng-Yao(1)
- Lončar, Biljana(1)
- Nikolić, Filip(1)
- Ozturk, Ahmet(1)
- Ozturk, Burhan(1)
- Uba, Abdullahi Ibrahim(1)
- Uysal, Sengul(1)
- Yagi, Sakina(1)
- Yapıcı, Ismail(1)
- Yıldız, Kenan(1)
Yayınlar
- MakaleAçık Erişim2026Scopus SJRQ1A multi-evidence computational and experimental strategy for Silene nerimaniae: combining phytochemical characterization, bioactivity assessment, and ADEFRank-HCT-116-based metabolite prioritization
Erharman, Ahmet; Yagi, Sakina; Uba, Abdullahi Ibrahim; Nikolić, Filip; Gašić, Uroš; Acar, Mikail; Dogan, Nurettin; Koyuncu, Ismail; Yapıcı, Ismail; Gulcin, Ilhami
This study presents the first comprehensive evaluation of the phytochemical composition, antioxidant properties, enzyme inhibitory effects, and cytotoxic potential of Silene nerimaniae G.E.Genç, Kandemir & İ.Genç (family Caryophyllaceae). Ethyl acetate, acetone, 70% acetone, and aqueous extracts were obtained from the aerial parts of the plant through maceration. Among the tested extracts, the acetone extract contained the highest total phenolic (24.37 mg GAE per g) and flavonoid (24.64 mg RE per g) contents. LC–MS analysis identified 52 bioactive constituents, including sugars, organic acids, phenolic acids, flavonoids, and fatty acids. The aqueous extract demonstrated the strongest radical scavenging activity against DPPH (23.02 mg TE per g) and ABTS (55.76 mg TE per g), as well as the highest Fe3+ reducing power (37.53 mg TE per g). In combination with the acetone extract, it also exhibited notable Cu2+ reducing activity (47.78 mg TE per g). Regarding enzyme inhibition, the ethyl acetate, acetone, and hydroacetone extracts displayed the strongest inhibitory activities against acetylcholinesterase (1.87 mg GALA per g), butyrylcholinesterase (2.92 mg GALA per g), and tyrosinase (60.44 mg KAE per g), respectively. All three extracts also inhibited α-amylase activity within the range of 0.73–0.77 mmol ACAE per g (p ≥ 0.05). In addition, the hydroacetone extract showed considerable inhibition of human carbonic anhydrase isoenzymes I (IC50 = 38.78 μg mL−1) and II (IC50 = 19.59 μg mL−1). The ethyl acetate extract exhibited the strongest cytotoxic effect against the colorectal cancer cell line HCT-116, with an IC50 value of 0.639 μg mL−1 and a selectivity index of 1.7. To contextualize this HCT-116-associated activity at the metabolite level, an ADEFRank-HCT116 (abundance–domain–evidence fusion ranking) workflow was applied by integrating EtOAc abundance, HCT-116-oriented QSAR evidence, applicability-domain confidence, and structure-assignment reliability. This analysis prioritized diosmetin, luteolin, eriodyctiol, acacetin, and isorhamnetin as core candidates for structure-based follow-up. Molecular docking screening predicted favorable docking profiles for the prioritized candidates, while 100 ns molecular dynamics simulations were used to assess the dynamic stability of the representative luteolin–PI3Kα, diosmetin–AKT1, and isorhamnetin–GSK-3β complexes. Taken together, these results indicate that S. nerimaniae may represent a valuable source of active compounds for various functional nutraceutical, cosmetic and pharmaceutical uses. This journal is
- MakaleAçık Erişim2026Scopus SJRQ1Optimization of ultrasound-assisted extraction and biological activities of Crataegus monogyna Jacq. flowering branches using experimental design and artificial neural networks
Uysal, Sengul; Cvetanović Kljakić, Aleksandra; Lončar, Biljana; Zengin, Gokhan; Cakilcioglu, Ugur
Crataegus monogyna is widely used in traditional medicine due to its rich content of bioactive compounds. The present study aimed to evaluate the influence of extraction parameters, including solvent concentration, extraction time, temperature, and solid-to-liquid ratio, on total phenolics, total flavonoids, antioxidant DPPH (2,2-diphenyl-1-picrylhydrazyl), ABTS (2,2′-azino-bis(3-ethylbenzothiazoline-6-sulphonic acid), FRAP (ferric reducing antioxidant power), and CUPRAC (cupric reducing antioxidant capacity)), and enzyme inhibitory properties (α-amylase and tyrosinase). Furthermore, an Artificial Neural Network (ANN) model was applied to optimize the extraction conditions. The results demonstrated that extraction conditions significantly affect both chemical composition and biological activity. The optimal conditions (70% ethanol, 30 min, 45 °C, (1 : 30)) yielded extracts with high total phenolic (109.15 mg GAE per g), and total flavonoid content (70.74 mg RE per g), strong antioxidant activity (DPPH: 473.57 mg TE per g, ABTS: 537.58 mg TE per g, CUPRAC: 407.11 mg TE per g, FRAP: 393.91 mg TE per g) and notable enzyme inhibitory potential (0.53 mmol ACAE per g against α-amylase and 75.00 mg KAE per g against tyrosinase). The ANN model showed excellent predictive performance, confirming its suitability for modeling and optimization of complex extraction systems. The present study provides comprehensive insights into optimized ultrasound-assisted extraction methods for the recovery of maximum bioactive compounds and biological activities (antioxidant and enzyme inhibition effect) from C. monogyna flowering branches using experimental design and ANN model. These findings provide a reliable basis for the development of functional and pharmaceutical products. This journal is
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İncele - MakaleAçık Erişim2026Scopus SJRQ1Natural propolis-based coatings as an effective strategy to control microbial growth and quality losses of kiwifruit during cold storage
Ozturk, Burhan; Gun, Sefa; Erturk, Omer; Aglar, Erdal; Yıldız, Kenan; Ozturk, Ahmet
Background: this study investigated the effects of ethanol-extracted propolis applications on the quality characteristics, microbial growth, and microstructural changes of kiwifruit (Actinidia deliciosa) during cold storage. Four different application groups were created: control, 70% ethanol application (ETH), 3% propolis coating (PRO1), and 6% propolis coating (PRO2). After dip coating, the fruits were stored for 150 days at 0 ± 0.5 °C and 90 ± 5% relative humidity, and analyses were performed on days 0, 30, 60, 90, 120 and 150. During storage, weight loss, respiration rate, fruit firmness, color, soluble solids content (SSC), titratable acidity (TA), vitamin C, total phenolics, total flavonoids, antioxidant activity (DPPH and FRAP assays), individual phenolic compounds, and microbial growth were determined in fruit samples. In addition, microstructural changes on the fruit surface were examined using scanning electron microscopy (SEM), and the elemental surface composition was determined using energy dispersive X-ray spectroscopy (EDX). Results: propolis coating applications maintained the quality characteristics of kiwifruit during storage. In particular, PRO1 and PRO2 reduced weight loss and respiration rate, better preserved fruit firmness, and maintained more stable color parameters. Propolis coatings also contributed to the preservation of ascorbic acid, total phenolic compounds, and total flavonoids, ensuring that antioxidant activity remained at higher levels throughout the storage period. Microbiological analyses showed that propolis coatings markedly reduced total bacterial counts on the kiwifruit surface, indicating their potential to limit microbial contamination during cold storage. SEM images showed that propolis coatings formed a homogeneous film layer on the fruit surface and limited microbial colonization by sealing surface pores. EDX analyses showed treatment-dependent changes in the surface elemental composition of kiwifruit peel, whereas SEM observations confirmed the formation of a continuous propolis-derived surface layer. Conclusions: overall, ethanol-extracted propolis coatings reduced total bacterial counts and quality losses in kiwifruit during cold storage and may serve as an effective natural preservative for extending cold-storage life. This journal is
AtıfScopus1OpenAlex1İncele - DergiRSC Medicinal Chemistry
- DergiRSC Advances
- DergiSustainable Food Technology