Sürdürülebilir Kalkınma Amaçları

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Yazarlar

• Acquaviva, Alessandra(1)
• Akev, Nuriye(1)
• Aksoy-Sagirli, Pinar(1)
• Alp-Kavlo, Hevidar(1)
• Altiparmak-Ulbegi, Gulsum(1)
• Arcan, Gulsah Gamze(1)
• Brunetti, Luigi(1)
• Cakilcioğlu, Ugur(1)
• Can, Ayse(1)
• Chiavaroli, Annalisa(1)
• Cicia, Donatella(1)
• Di Simone, Simonetta Cristina(1)
• Elkiran, Omer(1)
• Ferrante, Claudio(1)
• Izzo, Angelo Antonio(1)
• Leone, Sheila(1)
• Libero, Maria Loreta(1)
• Menghini, Luigi(1)
• Mukemre, Muzaffer(1)
• Nilofar(1)
• Orlando, Giustino(1)
• Ozsoy, Nurten(1)
• Recinella, Lucia(1)
• Seliman, Thoiba Mogahid Hussein(1)
• Uba, Abdullahi Ibrahim(1)
• Zengin, Gokhan(1)

• MakaleYalnız künye2026Scopus SJRQ3/r/munzur/1370
  A new lectin from Amanita vaginata mushroom with potent anti-inflammatory activity

  Seliman, Thoiba Mogahid Hussein; Ozsoy, Nurten; Altiparmak-Ulbegi, Gulsum; Alp-Kavlo, Hevidar; Arcan, Gulsah Gamze; Akev, Nuriye; Can, Ayse; Aksoy-Sagirli, Pinar

  This study used 80% ammonium sulphate fractional precipitation, followed by affinity chromatography to purify a new lectin (AVL) from the crude extract of the Amanita vaginata mushroom. The purification procedure achieved a 20% yield, a specific activity of 5120 HU/mg protein, and a 16-fold increase in purity. SDS-PAGE analysis showed a single band of 45 kDa. We assessed AVL's anti-inflammatory effect by measuring levels of inducible nitric oxide synthase (iNOS) and cyclooxygenase-2 (COX-2), induced by lipopolysaccharide (LPS), in RAW 264.7 macrophages. Western blot analysis showed that iNOS and COX-2 protein levels were barely detectable in the control groups, but markedly elevated in the LPS-treated groups. However, AVL treatment significantly inhibited the expression of both iNOS and COX-2 in LPS-stimulated cells. This effect was dose-dependent. These findings suggest that AVL's anti-inflammatory effects are mediated through the suppression of iNOS and COX-2 expression.

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• MakaleAçık Erişim2023Scopus SJRQ1/r/munzur/2063
  Adding New Scientific Evidences on the Pharmaceutical Properties of Pelargonium quercetorum Agnew Extracts by Using In Vitro and In Silico Approaches

  Chiavaroli, Annalisa; Libero, Maria Loreta; Di Simone, Simonetta Cristina; Acquaviva, Alessandra; Nilofar; Recinella, Lucia; Leone, Sheila; Brunetti, Luigi; Cicia, Donatella; Izzo, Angelo Antonio

  Pelargonium quercetorum is a medicinal plant traditionally used for treating intestinal worms. In the present study, the chemical composition and bio-pharmacological properties of P. quercetorum extracts were investigated. Enzyme inhibition and scavenging/reducing properties of water, methanol, and ethyl acetate extracts were assayed. The extracts were also studied in an ex vivo experimental model of colon inflammation, and in this context the gene expression of cyclooxygenase-2 (COX-2) and tumor necrosis factor α (TNFα) were assayed. Additionally, in colon cancer HCT116 cells, the gene expression of transient receptor potential cation channel subfamily M (melastatin) member 8 (TRPM8), possibly involved in colon carcinogenesis, was conducted as well. The extracts showed a different qualitative and quantitative content of phytochemicals, with water and methanol extracts being richer in total phenols and flavonoids, among which are flavonol glycosides and hydroxycinnamic acids. This could explain, at least in part, the higher antioxidant effects shown by methanol and water extracts, compared with ethyl acetate extract. By contrast, the ethyl acetate was more effective as cytotoxic agent against colon cancer cells, and this could be related, albeit partially, to the content of thymol and to its putative ability to downregulate TRPM8 gene expression. Additionally, the ethyl acetate extract was effective in inhibiting the gene expression of COX-2 and TNFα in isolated colon tissue exposed to LPS. Overall, the present results support future studies for investigating protective effects against gut inflammatory diseases.

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  AtıfScopus35·OpenAlex35

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