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- MakaleAçık Erişim2024Scopus SJRQ3The effects of biosynthesized silver nanoparticle on oxidative stress parameters in model organism Artemia salina (Linnaeus, 1758)
Ünal, İlkay; Ertürk Gürkan, Selin
Evaluation of the effects of nanomaterials in various habitats and living groups is increasing with in vitro and in vivo studies. This study aims to describe a biological procedure to synthesize silver nanoparticles using an extract of Marrubium astracanicum and to determine the aquatic environment’s oxidative stress on Artemia salina. Silver nanoparticle formation was visually observed with color change. Its structural properties, such as size, shape, morphology, and stability, were characterized by UV-Vis spectrophotometer, TEM, SEM, XRD, and DLS analyses. TEM image showed that the synthesized AgNPs have a size of between 20 and 50 nm. The effects of biosynthesized silver nanoparticles at different concentrations (control,0.2, 1, 5, 10, 25, and 50 mg/mL) and exposure times (24, 48 and 72 h) on total glutathione (GSH) and lipid peroxidation levels (MDA) were investigated on the Artemia salina organism, which is one of the most common saltwater organisms frequently used in ecotoxicity tests.
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İncele - MakaleAçık Erişim2022Anatomical, Micromorphological, Karyological and Biochemical study of Scutellaria orientalis subsp. virens and Scutellaria salviifolia
Acar, Mikail; Tasar, Neslihan; Akbulut, Guelcin Beker
In this study, the anatomical and micromorphological structure, karyological characteristics and biochemical content of Scutellaria orientalis subsp. virens and endemic Scutellaria salviifolia, whose distributions areas overlap, were compared. Some anatomical and micromorphological differences were observed on the taxa; scleranchymatic pericycle layer on the stem, stomata density, distribution of trichomes, as well as the main vascular bundle and general shape of the petiole. The chromosome numbers of both taxa were determined as 2n = 22. However, there was a difference between chromosome length range and total chromosome length. The chromosome numbers and chromosome morphologies of these species have been defined for the first time in this paper. Differences in biochemical content were observed between species. Chlorophyll a (Chl a), total chlorophyll (Total Chl), total carbohydrate and malondialdehyde (MDA) contents were determined higher in leaf and stem samples of S. orientalis subsp. virens than S. salviifolia. There was no significant difference between the two taxa in terms of chlorophyll b (Chl b) content. Carotenoid (Car) content was detected higher in leaves samples of S. orientalis subsp. virens, but no significant difference was found between stems samples. Also, the effect of taxa on biochemical contents in relation to the habitat they live in is given in this study.
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İncele - MakaleAçık Erişim2018Scopus SJRQ4Effect of glyphosate on some protective systems in Zea mays L; [Glifosatın mısır (Zea mays L.)’da bazı koruyucu sistemlere etkisi]
Beker Akbulut, Gulcin; Yigit, Emel; Bayram, Dilek
Glyphosate is an herbicide that is applied after non-selective germination and affects plant growth. In this study, glyphosate was applied to Zea mays L. after germination, at a concentration range from 0.017 to 0.145 M in a growth chamber. The effects of this herbicide on some antioxidant enzymes, lipid peroxidation, total chlorophyll and total carbohydrate content were investigated on days 1, 5 and 10 following the application. Results showed that peroxidase (POD), ascorbate peroxidase (APX), superoxide dismutase (SOD), catalase (CAT) and glutathione-S-transferase (GST) were increased in the groups treated with glyphosate on days 5 and 10, but the activities of reduced glutathione (GSH) and glutathione reductase (GR) were decreased on day 10. Malondialdehyde (MDA) content indicating lipid peroxidation was increased on days 1, 5 and 10. Changes in total chlorophyll and total carbohydrate contents were found to be time-dependent. These increases and decreases in the antioxidant activities, MDA content, total chlorophyll and total carbohydrate content in Zea mays L. were determined as a symptom of cytotoxicity caused due to glyphosate.
AtıfScopus3OpenAlex1İncele - MakaleAçık Erişim2018Scopus SJRQ3Effects of conventional and organic rearing systems and hen age on oxidative stress parameters of blood and ovarian tissues in laying hens; [Konvansiyonel ve organik yetiştirme sistemleri ile tavuk yaşının yumurta tavuklarının kan ve yumurtalık dokusundaki oksidatif stres parametreleri üzerine etkisi]
Şimşek, Ülkü Gülcihan; Baykalir, Yasin; Erişir, Mine; Benzer, Fulya
In this study, effects of hen age and rearing systems on some oxidative stress parameters in serum and ovarian tissues of hens are investigated. For this purpose, total of 48 Bovans White commercial laying hybrids from two different rearing systems (conventional cage and organic) at 30 and 60 weeks of age were used. Serum and ovarian tissues of the hens were collected following slaughter process and malondialdehyde (MDA), glutathione (GSH), glutathione peroxidase (GSH-Px) and catalase (CAT) levels of serum and ovarian tissues were determined. MDA levels of serum was found significantly higher in conventional system, both in 30 and 60 weeks old (P<0.05). GSH-Px activity was lower, conversely CAT activity was significantly higher in conventional system (P<0.05). Hen age didn’t affect MDA, GSH, GSH-Px and CAT levels of serum (P>0.05). In addition, there weren’t any significant interactions between rearing system and hen age in serum (P>0.05). MDA (P=0.010) and GSH (P<0.01) levels of ovarian tissue were found significantly higher in conventional system while GSH levels of ovarian tissue had increased in 60 weeks old (P<0.01). GSH-Px and CAT activity was similar between groups (P>0.05). Interaction between rearing system and hen age affected activity of CAT in ovarian tissue (P<0.05). As a result, conventional battery cages caused oxidative stress interrelated with increasing lipid peroxidation and MDA levels in laying hens. Antioxidant enzymes that play role in reduction of MDA were inadequate in this system. Oxidant/antioxidant balance can be established at 30 or 60 weeks of age by hen organism and therefore MDA levels of tissues would not be affected by age.
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