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- MakaleAçık Erişim2024Anti-inflammatory activity of a novel lectin isolated from Pleurotus eryngii var. ferulae mushroom
Seliman, Thoiba Mogahid Hussein; Ülbegi, Gulsum Altiparmak; Ozsoy, Nurten; Kavlo, Hevidar Alp; Sagirli, Pinar Aksoy
Background and Aims: Pleurotus species are edible mushrooms with important economic and medicinal value. Many pharmacological properties of these species, such as anticancer, immunomodulating, and anti-inflammatory activities, have been attributed to lectin. The aim of this study was to purify a novel lectin from Pleurotus eryngii var. ferulae (PEFL) and investigate its anti-inflammatory activity. Methods: PEFL was purified by 80% ammonium sulphate fractionation, diethylaminoethyl (DEAE)-Sepharose-4B anion exchange, and Sephadex G-100 gel filtration chromatography. The molar mass of the purified lectin was determined by sodium dodecyl sulphate-polyacrylamide gel electrophoresis (SDS-PAGE) under denaturing conditions. Inhibition of lectin-induced haemagglutination by several carbohydrates and one glycoprotein (ovalbumin) was also performed using the haemagglutination inhibition activity test. The anti-inflammatory effect of PEFL was tested in a lipopolysaccharide (LPS)-induced inflammation model in mouse macrophage cells (RAW 264.7). The levels of prostaglandin (PG)-E2, tumour necrosis factor-alpha (TNF-alpha), interleukin1-beta (IL-1 beta), interleukin-6 (IL-6), interleukin-10 (IL-10), and interferon-gamma (IFN-gamma) were determined using enzyme-linked immunosorbent assay (ELISA) kits. Furthermore, the expression of nitric oxide synthase (iNOS) and cyclooxygenase-2 (COX-2) was estimated by western blot analysis. Results: PEFL was obtained in 16% yield. The specific activity of PEFL was calculated as 1280 haemagglutinating units (HU)/mg protein and was inhibited only by D-galactose. The molecular weight was determined to be 46 kDa. PEFL showed an anti-inflammatory effect by reducing the production of pro-inflammatory PGs through COX-2 inhibition, as well as reducing iNOS expression. Furthermore, our findings reported that PEFL has a protective effect on inflammation by decreasing the production of pro-inflammatory cytokines and increasing the production of the anti-inflammatory cytokine (IL-10). Conclusion: The results suggest that PEFL can be considered as a potential therapeutic agent in the development of new therapeutic strategies for inflammatory diseases.
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İncele - MakaleAçık Erişim2018The antiiflammatory effect of Ginkgo biloba in lipopolysaccharide-induced rat sepsis model
Ilhan, Nevin; Susam, Solmaz; Ak, Tuba Parlak; Baykalir, Burcu Gul
Background: Sepsis is characterized dysregulated inflammatory response and is the leading cause of organ failure or injury and death in critical care units. Ginkgo biloba extract 761 (EGb 761) is characterized by antioxidant activity. There is no clear data in the functional role of EGb 761 that inhibiting of proinflammatory cytokines exist in sepsis. The purpose of this study was to investigate the antiinflammatory effect of EGb 761 on the levels of vascular endothelial growth factor (VEGF), matrix metalloproteinase-9 (MMP-9) and inducible nitric oxide synthase (iNOS) in lipopolysaccharide (LPS) induced rat sepsis model. Methods: The animals were randomly divided into the following six groups: the control, LPS, flunixin meglumine (FM), EGb 761, LPS + FM and LPS + EGb 761 groups. Enzyme-linked immunosorbent assay (ELISA) analysis was applied to measure serum levels of VEGF, MMP-9, and iNOS. Results: Serum iNOs (p < 0.05), VEGF (p < 0.01) and MMP-9 (p < 0.05) levels were significantly higher in the sepsis group. Treatment with EGb 761 decreased serum iNOs levels (p < 0.01), VEGF and MMP-9 levels decreased significantly (p < 0.05). Also, treatment with FM decreased iNOs (p < 0.01), VEGF and MMP-9 levels significantly (p < 0.05). Conclusions: It is suggested that sepsis may lead to increase serum iNOs, MMP-9, and VEGF levels and treatment of EGb 761 may have a potential antiinflammatory effect against LPS-induced sepsis.
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