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- MakaleAçık Erişim2023Scopus SJRQ3Bioremediation of herbicide diquat dibromide from aqueous solution with white rot fungus Phanerochaete chrysosporium
Yildirim, Numan; Ciftci, Emine; Erguven, Gokhan Onder; Durmus, Barbaros
In this study, the fungal bioremediation of herbicide diquat dibromide (DD) by white rot fungus Phanerochaete chrysosporium was studied. After bioremediation, the reduction in toxicity of the remediated media was evaluated by Daphnia magna as a model organism. For bioremediation studies, flask-level batch experiments were performed at different DD concentrations (10, 20 and 50 ppm) under static and agitated conditions. The highest reduction rates of chemical oxygen demand and total organic carbon were determined as 85.92 (for 20 ppm DD) and 87.46% (for 10 ppm DD), respectively under agitated conditions. After bioremediation, D. magna bioassay was used for toxicity assessment during 24, 48 and 72-h periods. In current study, it was observed that P. chrysosporium has very effective bioremediation capacity for diquat dibromide. The D. magna bioassay revealed that P. chrysosporium provides effective reduction in toxicity of diquat dibromide.
- Yüksek Lisans TeziAçık Erişim2022Beyaz çürükçül mantar Phanerochaete chrysosporium ile sulu çözeltiden Herbisit Dikuat Dibromür'ün biyoıslahı
Emine Çiftçi
Pestisitler toksik ve biyosidal maddeler olup çevre açısından önemli bir kirleticidir. Pestisitlerin kontrolsüz ve aşırı bir şekilde kullanımı ile depolanmaları, bertaraf edilmeleri esnasında yapılan yanlışlıklar çevre kirliliğine yol açmaktadır. Düşük konsantrasyonlarda bile uygulama alanlarından taşınabilir ve toprak hava, su ortamlarına geçerek ciddi çevre sorunlarına neden olabilmektedirler. Çalışmada sulu çözeltiden yabancı ve istenmeyen otlarla mücadelede kullanılan bir pestisit türü olan dikuat dibromür (DD) herbisitinin biyoıslahı (biyoiyileştirme) ve iyileştirme sonrası ortamın toksisitesi araştırılmıştır. Dikuat dibromürün biyoıslahı için bir beyaz çürükçül mantar türü olan Phanerochaete chrysosporium fungusu kullanılmıştır. 10, 20 ve 50 ppm konsantrasyonlarda hazırlanan ortamlardan 1, 3, 5 ve 7 günlük sürelerde örnekler alınmış, bu ortamlarda kimyasal oksijen ihtiyacı, toplam organik karbon ve mortalite değerleri incelenmiştir. Mortalite deneyinde su ortamında her türlü kimyasal değişime duyarlı olan Daphnia magna indikatör canlısı kullanılmıştır. Mortalite deneyinde 24, 48 ve 72 saatlik sürelerde D. magna bireylerinin hazırlanan deney ortamlarındaki ölüm oranları incelenmiştir. Yapılan çalışma sonucunda P. chrysosporium fungusunun sulu çözeltilerden dikuat dibromürün biyoiyileştirmesinde etkili olduğu ve giderim sonrası uygulanan toksisite deneyinde P. chrysosporium'un herbisit dikuat dibromürü daha toksik ürünlere çevirmeden verimli bir şekilde iyileştirilebildiği ortaya konmuştur.
- MakaleYalnız künye2013Scopus SJRQ3Determination of lethal concentration (LC50) values of Cinnamomum zeylanicum hydrosol on carp fish
Gulec, Kucukgul A.; Altinterim, B.; Aksu, O.
In this study, lethal concentration (LC50) values of cinnamon hydrosol (Cinnamomum zeylanicum) on carp (Cyprinus carpio) were investigated. In practice, experimental setup was constituted 30 fish (a total of 180 fish with 30 control fish) to be placed in three replicates. Hydrosol was added into aquariums at the doses of 0.5, 1, 2.5, 5 and 10% and determined mortality times of carp exposed to these concentrations. Percentage death of fish calculated in these concentrations. Mortality was observed at all treatments exception of dose of 0.5 %. The results indicate that the hydrosol had swimming changes, lethargy, lack of breath and leaning to the depth of the aquariums at all of the concentrations. The results of regression analysis indicated that the mortality rate (Y) is positively correlated the concentration (X) having a regression coefficient (R) close to one in each case. While the 1 h LC50 value (with 95% confidence limits) of the safe dose of C. zeylanicum hydrosol was estimated at LC50=4.39%, 2 h LC50 value=2.629%, and 12 h LC50 value= 1.027%.
AtıfWoS10İncele
- MakaleAçık Erişim2010Scopus SJRQ3Effects of cypermethrin on antioxidant status, oxidative stress biomarkers, behavior, and mortality in the freshwater mussel Unio elongatulus eucirrus
Köprücü, Kenan; Yonar, Serpil Mise; Seker, Engin
In this study, the effects of cypermethrin on antioxidant status, oxidative stress biomarkers, behavior, and mortality in the freshwater mussel Unto elongatulus eucirrus were examined. Cypermethrin was applied at concentrations of 5, 10, 20, 40, 80, and 160 mu g/L, for 1, 24, 48, 72, and 96 h. With increasing cypermethrin concentrations, the mussels showed significantly (P < 0.05) increased lipid peroxidation (MDA), which might be associated with decreased levels of reduced glutathione (GSH) and catalase (CAT) activity in the digestive glands and gills of the mussels. Negative correlations were observed between the MDA and the GSH and CAT levels after cypermethrin exposure, indicating a protective role of GSH and CAT against lipid peroxidation, and suggesting the use of these antioxidants as a potential biomarker of toxicity associated with contaminant exposure in freshwater mussels. In addition, at 5 mu g/L, cypermethrin had no effect on the duration of the active and rest periods. All higher concentrations caused inhibition of the filtration activity by reducing the active periods and lengthening the rest periods. The active periods shortened as the cypermethrin concentrations increased, the reduction being 90% of the control at 160 mu g/L. Rest periods were longest (237% longer than the control) at 160 mu g/L. The 48, 72, and 96 h LC50 values of cypermethrin for mussels were determined as 96.50, 77.96, and 59.20 mu g/L, respectively (P < 0.05). The results indicate that the insecticide cypermethrin has a harmful effect not only on nontarget aquatic arthropods and fish, but also on mollusks, although the sensitivity of mussels is less than that of fish. Being a general toxicant for aquatic life, cypermethrin should be used with great caution in agriculture to protect natural waters from contamination.
AtıfWoS34İncele


