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- MakaleAçık Erişim2026Scopus SJRQ1Ellagic Acid Mitigates Lead (Pb)-Induced Toxicity in Cyprinus carpio: A Multi-Biomarker Assessment of Hematological, Immunological, and Oxidative Stress Responses Supported by Molecular Docking
Eroglu, Mucahit; Cakmak, Mehmet Nuri; Pala, Aysegul; Uslu, Harun; Yonar, Serpil Mise; Ispir, Unal; Orhan, Cemal; Yonar, Muhammet Enis
Lead (Pb) is a widespread environmental pollutant that induces systemic toxicity in aquatic organisms primarily through oxidative stress, hematological disruption, and immune dysfunction. This study investigated the protective effects of ellagic acid (EA) against Pb-induced toxicity in common carp (Cyprinus carpio) using a multi-biomarker approach and molecular docking. Fish were assigned to six experimental groups: control, EA-treated, Pb-I, Pb-I + EA, Pb-II, and Pb-II + EA. Fish in the Pb-I and Pb-II groups were exposed to 2.5 and 5 mg/L Pb, respectively, while EA was administered via diet at 100 mg/kg for 14 days. At the end of the exposure period, hematological indices, innate immune parameters, and oxidative stress biomarkers were assessed in blood, liver, kidney, and gill tissues. Pb exposure caused marked hematological impairment, suppressed immune responses, increased malondialdehyde levels, and disrupted antioxidant defense by reducing SOD, CAT, GSH-Px, and GSH levels while increasing GST activity. In contrast, dietary EA supplementation significantly mitigated Pb-induced alterations, improved hematological and immunological responses, reduced lipid peroxidation, restored antioxidant capacity, and normalized GST activity to control levels. Molecular docking analyses further showed that EA interacts with hemoglobin and immunoglobulin M, supporting its potential role in preserving oxygen transport and immune functions under Pb-induced stress. Overall, the findings demonstrate that Pb-induced toxicity involves coordinated disruption of redox homeostasis and immune function, whereas EA exerts a multi-target protective effect through biochemical and molecular mechanisms. This study provides mechanistic insight into chemical-biological interactions and supports the potential application of natural bioactive compounds in mitigating heavy metal-induced toxicity.
- MakaleYalnız künye2015Scopus SJRQ3ANTIOXIDANT RESPONSES OF RAINBOW TROUT (Oncorhynchus mykiss) GILLS AFTER EXPOSURE TO HYDROGEN PEROXIDE
Seker, Engin; Ispir, Unal; Yonar, Serpil Mise; Yonar, M. Enis; Turk, Cebrahil
We evaluated antioxidant responses of Oncorhynchus mykiss, experimentally exposed to different concentrations of hidrogen peroxide (H2O2). Different concentrations of H2O2 (50 and 100 ppm) were administrated to the fish for 30, 60 and 90 min. Samples of the gill were collected at the end of the experiment, and analysed for their oxidant antioxidant status, including the malondialdehyde (MDA) level, the catalase (CAT) and glutathione peroxidase (GSH-Px) activity as well as the reduced glutathione (GSH) concentration. Results obtained showed that H2O2 significantly increased MDA level. Compared to controls, GSH level and GSH-Px and CAT activities were significantly reduced in the fish that were exposed to different concentrations of H2O2. The current data suggest that H2O2 has an oxidative-stress-inducing potential in fish. The various parameters studied in this investigation can also be used as biomarkers of exposure to H2O2.
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İncele - MakaleAçık Erişim2015Scopus SJRQ1Trichlorfon-induced haematological and biochemical changes in Cyprinus carpio: ameliorative effect of propolis
Yonar, M. Enis; Yonar, Serpil Mise; Pala, Aysegul; Silici, Sibel; Saglam, Naim
Trichlorfon is among the most commonly used products to treat fish parasites in aquaculture. We investigated the effectiveness of propolis in alleviating the toxicity of trichlorfon on haematological and oxidant/antioxidant parameters in carp Cyprinus carpio. Fish were exposed to sublethal concentrations (11 and 22 mg l(-1)) of trichlorfon, and propolis (10 mg kg(-1) of fish weight) was simultaneously administered. At the end of 14 d administration, blood and tissue (liver, kidney, gill) samples were collected. Haematological changes (red and white blood cell count, haemoglobin concentration, haematocrit level and erythrocyte indices: mean corpuscular volume, mean corpuscular haemoglobin and mean corpuscular haemoglobin concentration) were determined in the blood samples, while antioxidant parameters (malondialdehyde and reduced glutathione levels and superoxide dismutase, catalase and glutathione peroxidase activities) were evaluated in the liver, kidney and gill samples. Trichlorfon led to negative alterations in the haematological and antioxidant parameters investigated. The administration of propolis alleviated this effect and suggests that fish treated with trichlorfon improve their physiological status when fed a propolis-supplemented diet.
AtıfWoS46OpenAlex56
İncele - MakaleAçık Erişim2013Scopus SJRQ2The effect of dietary n-3 series fatty acids on the number of pleopadal egg and stage 1 juvenile in freshwater crayfish, Astacus leptodactylus Eschscholtz
Harlioglu, Muzaffer Mustafa; Çakmak, Mehmet Nuri; Köprücü, Kenan; Aksu, Onder; Harlioglu, Ayse Gul; Yonar, Serpil Mise; Duran, Tuba Cakmak; Özcan, Sinan; Gündogdu, Hande
The effect of dietary n-3 series fatty acids on the number of pleopadal egg and stage 1 juvenile in freshwater crayfish, Astacus leptodactylus was investigated. Crayfish were fed with n-3 series fatty acids supplemented diets and a control diet for 251days. Control diet did not comprise additional n-3 series fatty acids. However, D2, D3 and D4 groups were supplemented with n-3 series fatty acids at 1%, 2% and 3% level respectively. Results showed the beneficial effects of dietary n-3 series fatty acids on the production of pleopodal egg and stage 1 juvenile number. At the end of the experiment, pleopodal egg (from 177 to 234) and stage 1 juvenile number (from 167 to 225) increased significantly with increased dietary n-3 series fatty acid level. In addition, an increase in dietary n-3 series fatty acids led to an increase in number of pleopodal egg and stage 1 juvenile produced per gram of female crayfish weight. It can be concluded that the reproductive efficiency of A. leptodactylus (i.e., pleopodal egg and stage 1 juvenile number) can be improved in controlled hatchery conditions by adding n-3 series fatty acid into the diet of this species.
AtıfWoS22OpenAlex26İncele - MakaleÖn baskısı açık2013Scopus SJRQ2Effect of dietary n-3 series fatty acids on sperm production in the freshwater crayfish, Astacus leptodactylus (Eschscholtz) (Astacidae)
Harlioglu, Muzaffer Mustafa; Köprücü, Kenan; Harlioglu, Ayse Gul; Yonar, Serpil Mise; Duran, Tuba Cakmak; Çakmak, Mehmet Nuri; Aksu, Onder; Özcan, Sinan; Kutluyer, Filiz; Gündogdu, Hande
The objective of the present work was to evaluate the effects of three different rates of long chain n-3 polyunsaturated fatty acid (LC n-3 PUFA) on the sperm number of Astacus leptodactylus. The experiment was carried out with the four following treatments: control group (not supplemented LC n-3 PUFA), D1 (1 %), D2 (2 %) and D3 (3 %). The sperm number of A. leptodactylus was counted; the reproductive system was weighted; and the macroscopical analysis of the testes and vasa deferentia (DVD) were conducted. In addition, gonado-somatic index (GI) and testes index (TI) were also calculated. The results showed that there was a significant difference between the crayfish fed LC n-3 PUFA supplemented diets (D1, D2, D3) and control in mean sperm number (P < 0.05). The highest sperm number was found for the crayfish fed D3 (6.27 +/- A 0.14 x 10(8) sperm/distal vas deferens section). The results also showed that the reproductive system of the crayfish fed D3 and D2 was significantly heavier than that of the crayfish fed the control and D1 (P < 0.05). On the other hand, there was not a significant difference in the testes weight and vasa deferentia weight between the crayfish fed the control and D1, and between the crayfish fed D2 and D3 (P < 0.05). GI of the crayfish fed D3 and D2 was significantly higher than that of the crayfish fed control and D1 (P > 0.05), but there was not a significant difference in the TI of the crayfish fed control, D1, D2 and D3 (P > 0.05). In conclusion, diets containing different levels of LC n-3 PUFA significantly increase the sperm number of A. leptodactylus, and they are crucial for the production of higher number of sperm in this species.
AtıfWoS30OpenAlex34İncele - MakaleAçık Erişim2010Scopus SJRQ3Effects of cypermethrin on antioxidant status, oxidative stress biomarkers, behavior, and mortality in the freshwater mussel Unio elongatulus eucirrus
Köprücü, Kenan; Yonar, Serpil Mise; Seker, Engin
In this study, the effects of cypermethrin on antioxidant status, oxidative stress biomarkers, behavior, and mortality in the freshwater mussel Unto elongatulus eucirrus were examined. Cypermethrin was applied at concentrations of 5, 10, 20, 40, 80, and 160 mu g/L, for 1, 24, 48, 72, and 96 h. With increasing cypermethrin concentrations, the mussels showed significantly (P < 0.05) increased lipid peroxidation (MDA), which might be associated with decreased levels of reduced glutathione (GSH) and catalase (CAT) activity in the digestive glands and gills of the mussels. Negative correlations were observed between the MDA and the GSH and CAT levels after cypermethrin exposure, indicating a protective role of GSH and CAT against lipid peroxidation, and suggesting the use of these antioxidants as a potential biomarker of toxicity associated with contaminant exposure in freshwater mussels. In addition, at 5 mu g/L, cypermethrin had no effect on the duration of the active and rest periods. All higher concentrations caused inhibition of the filtration activity by reducing the active periods and lengthening the rest periods. The active periods shortened as the cypermethrin concentrations increased, the reduction being 90% of the control at 160 mu g/L. Rest periods were longest (237% longer than the control) at 160 mu g/L. The 48, 72, and 96 h LC50 values of cypermethrin for mussels were determined as 96.50, 77.96, and 59.20 mu g/L, respectively (P < 0.05). The results indicate that the insecticide cypermethrin has a harmful effect not only on nontarget aquatic arthropods and fish, but also on mollusks, although the sensitivity of mussels is less than that of fish. Being a general toxicant for aquatic life, cypermethrin should be used with great caution in agriculture to protect natural waters from contamination.
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