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- MakaleYalnız künye2016Scopus SJRQ3Combined effects of physicochemical variables (pH and salinity) on sperm motility: characterization of sperm motility in European sea bass Dicentrarchus labrax
Ögretmen, F.; Inanan, B. E.; Kutluyer, F.
Gamete activation in fish is an important step in terms of artificial fertilization of oocytes, cryopreservation studies and other experimental manipulations. Salinity and pH differences in activation media affect to sperm motility and fertilizing ability. These experiments were therefore designed to investigate the combined effects of pH (range 5.0-9.0) and salinity (20, 30, 37, and 45 parts per thousand) of activation media on sperm motility of European sea bass Dicentrarchus labrax. The best results were obtained at salinity 37 parts per thousand and a pH of 9.0. Our results also demonstrated that non-progressive motility at salinity 45 parts per thousand was observed in the range of 5.0-9.0 pH. In conclusion, spermatozoa can be motile at a wide range of pH and salinity values although the percent of motile spermatozoa and motility duration are negatively affected by low pH values.
AtıfWoS13OpenAlex17
İncele - MakaleYalnız künye2016Scopus SJRQ3EFFECTS OF SALINITY, LIGHT AND NITROGEN CONCENTRATION ON GROWTH AND LIPID ACCUMULATION OF THE GREEN ALGAE DUNALIELLA BARDAWIL
Can, Safak Seyhaneyildiz; Cirik, Semra; Koru, Edis; Turan, Gamze; Tekogul, Hatice; Subakan, Tugba
Efficient biofuel production from algae depends on the microalgal biomass production rate and lipid content. Both biomass production and lipid accumulation are limited by several factors, of which nutrients play a key role. In this study, the marine microalgae Dunaliella bardawil was used as a model organism. D. bardawil was cultured at two different sodium nitrate concentrations as 0 and 0.7 M, at six different amounts of salinity as 0.65, 1.0, 1.5, 2.0, 2.75 and 3.5 M, and at two different light intensity levels as 65 and 800 mu mol foton m(-2) s(-1) The study was done under pH 7. After 15-day growth period, the most efficient culture of D. bardawil for both biomass increase and lipid production was determined for the samples with 39.67 +/- 4.51 lipid rates cultured under 0.7 M nitrate concentration, 2.75 M salinity and 800 mu mol photon m(-2) sn(-1) light intensity. This high level of lipid production is sufficient to use D. bardawil for biodiesel production. Also after the evaluation of fatty acid results of GC-FID analysis, it was determined that D. bardawil contains high rate of medium length (C16-C18) fatty acid chains suitable for the production of biodiesel. These results are satisfactory to use D. bardawil in biodiesel production.
AtıfWoS2İncele