Sürdürülebilir Kalkınma Amaçları

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• Yüksek Lisans TeziAçık Erişim2018/r/munzur/7056
  Farklı oranlardaki demirin Astacus leptodactylus (Esch., 1823)'lardaki yaşama oranı, oksidatif stres, Fe, Cu, Cr ve Zn seviyeleri üzerine etkileri

  Hülya Şahin

  Bu çalışma 15 Nisan 2016 – 15 Temmuz 2016 tarihleri arasında yapıldı. Çalışmada, erkek tatlı su istakozu (Astacus leptodactylus) rasyonlarına farklı oranlarda demir (Fe) katılmasının; Astacus leptodactylus 'un yaşama oranı, hepatopankreas ve karapakstaki demir (Fe), bakır (Cu), çinko (Zn), krom (Cr) ve lipid peroksidasyon (Malondialdehit (MDA)) düzeyleri üzerine etkisinin ortaya konması amaçlandı. Bu amaçla, bir kontrol (K) ve dört adet deneme (F1, F2, F3, F4) rasyonu hazırlandı. Rasyon öğeleri kontrol rasyonuna 28,60 ± 2,21 mg kg-1 demir katkısı sağlamaktadır. Kontrol rasyonuna 50 mg kg-1, 100 mg kg-1, 200 mg kg-1 ve 300 mg kg-1 demir sülfat katılarak sırasıyla F1, F2, F3 ve F4 rasyonları oluşturuldu. Çalışma üç tekrar halinde gerçekleştirildi. Sonuç olarak, araştırmada Astacus leptodactylus kontrol rasyonuna farklı oranlarda demir katılmasının; Astacus leptodactylus 'un yaşama oranını etkilemediği görüldü. Dokulardaki Cr değeri standartların altında olduğundan dolayı tespit edilemedi. Karapaks da MDA değeri belirlenemedi. Hepatopankreasta MDA olarak belirlenen lipid peroksidasyon düzeyini önlemede kontrol rasyonuna 300 mg kg-1 demir ilavesinin yeterli olduğu bulundu. Ayrıca bu çalışmada yemdeki demir miktarının dokulardaki Fe, Cu ve Zn birikimi üzerinde etkili olduğu görüldü.

  İncele
• MakaleYalnız künye2017Scopus SJRQ3/r/munzur/4320
  MONTHLY VARIABILITY OF OXIDANT AND ANTIOXIDANT BIOMARKERS OF FRESHWATER CRAYFISH (ASTACUS LEPTODACTYLUS ESCH., 1823)

  Barim-Oz, Ozden; Yilmaz, Seval; Sahin, Hulya; Benzer, Fulya

  In the present study was determined the effects of monthly variability on enzymatic antioxidant (superoxide dismutase (SOD), catalase (CAT), glutathione peroxidase (GSH-Px)), antioxidant (reduced glutathione (GSH)) and the oxidant status (nitric oxide (NO), lipid peroxidation (MDA)) in hepatopancreas, muscle, gills and gonad tissues of freshwater crayfish (A. leptodactylus) the monthly collected throughout the year from Keban Dam Lake of Turkey. Results indicate that variations in oxidants and antioxidants with relation to month were not only tissue specific but also were gender specific. The levels of oxidant parameters were generally higher in hepatopancreas in comparison to muscle, gills and gonad of crayfish. Moreover, in tissues were also determined the different oxidants and antioxidants responses in moulting, mating, breeding and egg laying periods.

  AtıfWoS5

  İncele
• MakaleYayıncıda açık erişim2016Scopus SJRQ3/r/munzur/4789
  Oxidative stress and some biochemical parameters during starvation and refeeding in Astacus leptodactylus (Esch., 1823)

  Barim-Oz, O.; Sahin, H.

  This study was conducted to determine the effect of starvation (78 days) and refeeding (33 days) on oxidative stress (malondialdehyde (MDA)), antioxidants (superoxide dismutase (SOD), catalase (CAT), glutathione reductase (GR), glutathione peroxidase (GSH-Px)), arginase (AR) and nitric oxide (NO) in the hepatopancreas, muscle and gills tissues of freshwater crayfish (Astacus leptodactylus). Crayfish were maintained at three experimental groups: control (fed), starved (not fed) crayfish for 78 days and refeeding crayfish for 33 days after 78 days starvation. The biochemical analysis in the tissues were measured at 3, 18, 33, 48, 63 and 78 days of starvation and feeding and at 3, 18 and 33 days of refeeding. In the all periods, the MDA levels were significantly higher in tissues of starved crayfish than the control ones. Significant changes in the MDA levels were also observed amongst starvation times. In the tissues of starved crayfish was significantly higher SOD, CAT, GR and AR activities when compared to control crayfish. The GSH-Px and NO activity showed increases and decreases in different starvation periods according to control group. The parameters measured returned to control values after 33 days of the refeeding. Collectively, the present study indicated that starvation induces MDA, SOD, CAT, GSH-Px, GR, AR and NO activity in the tissues, and A. leptodactylus exposed to starvation can be withstand 78 days.

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  AtıfWoS8·OpenAlex14

  İncele
• MakaleAçık Erişim2016Scopus SJRQ3/r/munzur/4642
  The influence of dietary antioxidant on ovarian eggs and levels of vitamin E, C, A, astaxanthin, β-carotene and oxidative stres in tissues of Astacus leptodactylus (Eschscholtz)

  Barim-Oz, O.; Sahin, H.

  The experiment was conducted to determine the most effective antioxidant (among the vitamin E (VE), vitamin C (VC), vitamin A (VA), astaxanthine (AX), beta-carotene (beta C)) on the ovarian egg number and size, level of VE, VC, VA, AX, beta C and oxidative stress (as malondialdehyde (MDA)) in the hepatopancreas, ovarian, gills and muscle tissue during ovarian development of Astacus leptodactylus. One control (C) and five experimental diets (EE, EC, EA, EAX and E beta C) were prepared. The EE, EC, EA, EAX and E beta C groups were formed by added 150 mg kg-1 VE, 200 mg kg-1 VC, 240 mg kg-1 VA, 200 mg kg-1 AX and 200 mg kg-1 beta C to diet C, respectively. At the end of the experiment found that the dietary antioxidants increased ovarian egg number and size and reduced the level of MDA in the tissues. Ovarian egg number and size were highest in the EE and EAX diet groups in the comparison to control (p< 0.001). The level of MDA in the tissues was lowest in the EAX diet group in the comparison to control (p< 0.001). The highest levels of VE, VC, VA, AX and beta C were found in the hepatopancreas and ovarian compared with muscle and gills. The highest level of MDA also was determined in the ovarian according to other tissues. In conclusion, the VE and AX in broodstock diets were the most effective antioxidants on the ovarian egg number and size of A. leptodactylus.

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  AtıfWoS16·OpenAlex53

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