Sürdürülebilir Kalkınma Amaçları

• 3

• MakaleAçık Erişim2020Scopus SJRQ3/r/munzur/3712
  Antioxidant effects of oleuropein on hydrogen peroxide-induced neuronal stress-an in vitro study

  Kucukgul, Altug; Isgor, Mehmet M.; Duzguner, Vesile; Atabay, Meryem N.; Kucukgul, Azime

  Background: Persistent oxidative stress can lead to chronic inflammation and mediate most chronic diseases including neurological disorders. Oleuropein has been shown to be a potent antioxidant molecule in olive oil leaf having antioxidative properties. Objective: The aim of this study was to investigate the protective effects of oleuropein against oxidative stress in human glioblastoma cells. Methods: Human glioblastoma cells (U87) were pretreated with oleuropein (OP) essential oil 10 µM. After 30 minutes, 100 µM H2 O2 was added to the cells for three hours. Cell survival was quantified by colorimetric MTT assay. Glutathione level, total oxidant capacity, total antioxidant capacity and nitric oxide levels were determined by using specific spectrophotometric methods. The relative gene expression level of iNOS was performed by qRT-PCR method. Results: According to viability results, the effective concentration of H2 O2 (100µM) significantly decreased cell viability and oleuropein pretreatment significantly prevented the cell losses. Oleuropein regenerated total antioxidant capacity and glutathione levels decreased by H2 O2 exposure. In addition, nitric oxide and total oxidant capacity levels were also decreased after administration of oleuropein in treated cells. Conclusion: Oleuropein was found to have potent antioxidative properties in human glioblastoma cells. However, further studies and validations are needed in order to understand the exact neuroprotective mechanism of oleuropein.

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  AtıfScopus23·OpenAlex24

  İncele
• MakaleYalnız künye2019Scopus SJRQ3/r/munzur/4646
  Bio-functions of carvacrol-supplemented feeds on lipopolysaccharide-induced rainbow trout (Oncorhynchus mykiss Walbaum, 1792)

  Kucukgul, A.; Gulsafak, I

  To investigate the effects of carvacrol supplementation in rainbow trout induced by lipopolysaccharide (LPS), relative gene expressions levels of some pro-inflammatory cytokines and apoptosis markers in kidney and liver were measured. Fish with a mean weight of 44.71 +/- 1.33 g were studied using four different treatments with three replicates each. Inflammation and apoptosis were performed using LPS of Escherichia coli (25 mu g ml(-1)) except in the control group and only carvacrol (100 mu g ml(-1) diet) containing diets were fed to this group (CAR). The last group was the infected fish fed carvacrol supplemented diet (+CAR). Kidney and liver tissues were removed 3 days after to determine the levels of interleukin-1 beta (IL-1 beta), interferon gamma (INF-gamma), caspase 3 (Cas 3), caspase 8 (Cas 8) using Real-Time PCR analyses. IL-1 beta expressions of both kidney and liver was significantly decreased (12.9 and 2.14 fold, respectively) in LPS treated cells (p<0.05). While IFN-gamma expression was up regulated in kidney, it had down regulation in liver. LPS decreased both Cas 3 and 8 expressions in kidney but increased in liver. +CAR increased expressions of IL-1 beta and IFN-gamma compared with the control (up to 2-3 fold) in all tissues except caspase gene expressions that were similar in the control. In all tissues, IL-1 beta and IFN-gamma expressions increased in +CAR group, expect IFN-gamma in the kidney. However, Cas 3 and 8 expressions including apoptosis was induced by up-down regulations in all tissues compared to LPS-injected fish. The results showed that carvacrol had pro-inflammatory and apoptotic effects especially on liver tissue of LPS-induced inflammation model of Oncorhynchus mykiss.

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  AtıfWoS4·OpenAlex3

  İncele
• MakaleYalnız künye2019Scopus SJRQ2/r/munzur/3527
  Investigation of the anti-apoptotic activity of ozone therapy in rainbow trout macrophages infected with Yersinia ruckeri

  Küçükgül, Azime; Küçükgül, Altuğ; Gönenci, Ramazan; Özsoy, Şule Yurdagül; Kutlu, Banu; Işgör, Mehmet Mustafa

  Yersinia ruckeri is the causal agent of enteric redmouth (ERM) in trout. This study aimed to investigate the effectiveness of nontoxic ozone on Y. ruckeri-infected trout cells in in vitro conditions. Rainbow trout macrophages (RTS-11) were exposed to Y. ruckeri for 24 h after being incubated with the ozonized medium at nontoxic concentration. Effective concentrations of nontoxic ozone (3 gamma) and bacteria (1.5 x 108 cfu/mL) were determined by viability tests. The expression of caspases 1, 3, 8, and 12 was determined by real-time PCR and protein levels investigated by ELISA method. Apoptotic-necrotic cell ratios were determined by acridine orange and ethidium bromide staining. Bacterial stimulations of caspases (1, 3, 8, and 12) were suppressed by ozone mostly at the translational level. While bacterial infection increased the count of apoptotic and necrotic cells at different stages, ozone significantly reduced this condition. Obtained data indicate possible use of nontoxic ozone as a safe and effective treatment alternative for increasing host defenses and eliminating the infection in the case of yersiniosis in fish.

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  AtıfWoS1·OpenAlex3

  İncele
• MakaleYalnız künye2013Scopus SJRQ2/r/munzur/4672
  THERAPEUTIC EFFECTS OF THYME (THYMUS VULGARIS LINNEAUS) AND FENNEL (FOENICULUM VULGARE MILLER) ESSENTIAL OILS IN INFECTED RAINBOW TROUT, ONCORHYNCHUS MYKISS (WALBAUM)

  Gulec, A. Kucukgul; Kucukgul, A.; Danabas, D.; Ural, M.; Seker, E.; Arslan, A.; Serdar, O.

  The fish (84 +/- 1.02 g mean +/- SEM) were divided into 3 groups (N=15 in each group) as control (CNT), infected with Yersinia ruckeri (INF), and infected and essential oils (+EO). The CNT and INF groups were fed only with commercial fish feed. The +EO group was fed with feeds prepared with the rate of 10 ml herbal essential oils (mixed thyme and fennel) / 100 g feed during 21 days. The liver tissue (0.4 g) of fish was dissected in third day of the experimental period and mRNA transcription levels in liver tissue were determined by Real Time PCR analysis. INF and +EO groups exhibited decreased immunity against the challenging with Y. ruckeri infection (53.57 and 42.85 % mortality, respectively) when compared with CNT group (93.33 %). Gene mRNA transcription levels of tumour necrosis factor alpha (TNF-alpha), interleukin 1 beta (IL-1 beta), inducible nitric oxide synthesis (iNOS), serum amyloid A (SAA), that are responsible during the initiation and continuation of the inflammation increased in 1.86, 2.46, 1.62 and 55.7 fold change, respectively in INF group, whereas mRNA expressions of haptoglobin (Hp) decreased (1.2-fold change) in 3th day of experimental period. The +EO group eliminated the increase in all of the gene expressions.

  AtıfWoS7

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