Sürdürülebilir Kalkınma Amaçları

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• MakaleYalnız künye2026Scopus SJRQ3/r/munzur/1370
  A new lectin from Amanita vaginata mushroom with potent anti-inflammatory activity

  Seliman, Thoiba Mogahid Hussein; Ozsoy, Nurten; Altiparmak-Ulbegi, Gulsum; Alp-Kavlo, Hevidar; Arcan, Gulsah Gamze; Akev, Nuriye; Can, Ayse; Aksoy-Sagirli, Pinar

  This study used 80% ammonium sulphate fractional precipitation, followed by affinity chromatography to purify a new lectin (AVL) from the crude extract of the Amanita vaginata mushroom. The purification procedure achieved a 20% yield, a specific activity of 5120 HU/mg protein, and a 16-fold increase in purity. SDS-PAGE analysis showed a single band of 45 kDa. We assessed AVL's anti-inflammatory effect by measuring levels of inducible nitric oxide synthase (iNOS) and cyclooxygenase-2 (COX-2), induced by lipopolysaccharide (LPS), in RAW 264.7 macrophages. Western blot analysis showed that iNOS and COX-2 protein levels were barely detectable in the control groups, but markedly elevated in the LPS-treated groups. However, AVL treatment significantly inhibited the expression of both iNOS and COX-2 in LPS-stimulated cells. This effect was dose-dependent. These findings suggest that AVL's anti-inflammatory effects are mediated through the suppression of iNOS and COX-2 expression.

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  AtıfScopus2·WoS1·OpenAlex2

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• MakaleAçık Erişim2024/r/munzur/4062
  Anti-inflammatory activity of a novel lectin isolated from Pleurotus eryngii var. ferulae mushroom

  Seliman, Thoiba Mogahid Hussein; Ülbegi, Gulsum Altiparmak; Ozsoy, Nurten; Kavlo, Hevidar Alp; Sagirli, Pinar Aksoy

  Background and Aims: Pleurotus species are edible mushrooms with important economic and medicinal value. Many pharmacological properties of these species, such as anticancer, immunomodulating, and anti-inflammatory activities, have been attributed to lectin. The aim of this study was to purify a novel lectin from Pleurotus eryngii var. ferulae (PEFL) and investigate its anti-inflammatory activity. Methods: PEFL was purified by 80% ammonium sulphate fractionation, diethylaminoethyl (DEAE)-Sepharose-4B anion exchange, and Sephadex G-100 gel filtration chromatography. The molar mass of the purified lectin was determined by sodium dodecyl sulphate-polyacrylamide gel electrophoresis (SDS-PAGE) under denaturing conditions. Inhibition of lectin-induced haemagglutination by several carbohydrates and one glycoprotein (ovalbumin) was also performed using the haemagglutination inhibition activity test. The anti-inflammatory effect of PEFL was tested in a lipopolysaccharide (LPS)-induced inflammation model in mouse macrophage cells (RAW 264.7). The levels of prostaglandin (PG)-E2, tumour necrosis factor-alpha (TNF-alpha), interleukin1-beta (IL-1 beta), interleukin-6 (IL-6), interleukin-10 (IL-10), and interferon-gamma (IFN-gamma) were determined using enzyme-linked immunosorbent assay (ELISA) kits. Furthermore, the expression of nitric oxide synthase (iNOS) and cyclooxygenase-2 (COX-2) was estimated by western blot analysis. Results: PEFL was obtained in 16% yield. The specific activity of PEFL was calculated as 1280 haemagglutinating units (HU)/mg protein and was inhibited only by D-galactose. The molecular weight was determined to be 46 kDa. PEFL showed an anti-inflammatory effect by reducing the production of pro-inflammatory PGs through COX-2 inhibition, as well as reducing iNOS expression. Furthermore, our findings reported that PEFL has a protective effect on inflammation by decreasing the production of pro-inflammatory cytokines and increasing the production of the anti-inflammatory cytokine (IL-10). Conclusion: The results suggest that PEFL can be considered as a potential therapeutic agent in the development of new therapeutic strategies for inflammatory diseases.

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  AtıfWoS1·OpenAlex2

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• MakaleYalnız künye2024Scopus SJRQ2/r/munzur/2205
  Process optimization and modeling of Pb(II) ions adsorption on a new and reusable biocomposite material using experimental design approach

  Ince, Muharrem; Kaplan Ince, Olcay; Alp Kavlo, Hevidar

  This research paper aimed to establish the optimal conditions for lead (Pb(II)) ions from industrial wastewater (IW) using response surface methodology and central composite design (CCD) approach. A fixed-bed column study was designed by using a novel and reusable biocomposite material, Amanita vaginata (A. vaginata) immobilized on Amberlite XAD-4 resin (A&XAD-4@AV), to find out biosorption behavior of Pb(II) ions. Various experimental variables such as optimum pH value, biocomposite material amount, flow rate, and Pb(II) ions initial concentration were investigated, which significantly affected the Pb(II) ions removal efficiency. The maximum Pb(II) removal efficiency of A&XAD-4@AV biocomposite material was determined as 11.2 mg g−1 under optimum conditions. The reusability of A&XAD-4@AV biocomposite material was found to be suitable. For checking model adequacy, a regression model was derived using CCD to predict the responses and analysis of variance (ANOVA) and lack of fit test was performed. It was observed that the quadratic model, which was controlled and proposed, originated from experimental design data. The ANOVA showed a high coefficient of determination (R2 = 0.9896, R2Adj = 0.9800, R2Pred = 0.9633). Finally, developed and optimized method was applied effectively to wastewater for determining Pb(II) ions levels. The current study indicated that the prepared A&XAD-4@AV is an efficient material to remove of Pb(II) ions from IW.

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  AtıfScopus1·WoS1·OpenAlex1

  İncele
• MakaleYalnız künye2023Scopus SJRQ3/r/munzur/2702
  Green Synthesis and Encapsulation of Superparamagnetic Magnetite for Mercury (II) Removal: Adsorption Isotherms, Adsorption Kinetics, and Thermodynamic Studies

  Alp Kavlo, Hevidar; Ince, Muharrem; Kaplan Ince, Olcay; Aydogdu, Burcu; Ozcan, Cemile

  Mercury (II) ions have significant ecological impacts. In the current study, magnetized iron oxide (Fe3O4) nanoparticles were green synthesized and encapsulated with alginate (ALG). The nanoparticles were converted into a composite (Fe3O4/ALG). The Fe3O4/ALG beads were used to remove Hg (II) from industrial wastewater. Characterization was performed by X-ray diffraction (XRD), scanning electron microscopy—energy dispersive spectroscopy (SEM-EDS), Fourier transform infrared spectroscopy (FT-IR), and thermogravimetric analysis-differential thermal analysis (TGA-DTA). A central composite design (CCD) approach was performed to define the experimental conditions providing the highest global adsorption efficiency and to optimize the pH, temperature and adsorbent mass. Hg (II) concentrations were determined by flame and electrothermal atomic absorption spectrometry (FAAS and ETAAS). The limits of detection (LOD) and quantification (LOQ) were 1.2 mg L−1 and 3.5 mg L−1 for FAAS and 0.2 µg L−1 and 0.5 µg L−1 for ETAAS, respectively. Additionally, Langmuir, Freundlich, and Temkin isotherm models were employed to determine the adsorption mechanism. The adsorption capacity of Fe3O4/ALG was determined to be 49.18 mg g−1 by the Langmuir model. Kinetic studies revealed that the adsorption of Hg (II) best fitted the pseudo-second order model. The Gibb’s free energy (ΔG°) values showed that the adsorption of Hg (II) on Fe3O4/ALG occurred spontaneously.

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  AtıfScopus8·WoS8·OpenAlex10

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