Sürdürülebilir Kalkınma Amaçları

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• MakaleYalnız künye2020Scopus SJRQ4/r/munzur/3625
  Biochemical responses of some (Helianthus annuus L.) cultivars to the interaction effects of biodegradable polymer and salt stress

  Akbulut, Gulcin Beker; Torgut, Gulben

  Salt stress is one of the most important environmental factor limiting plant growth and causing serious agricultural problems in the world. Biodegradable poly(ethylene oxide)-co-(ε-caprolactone) poly(EO-co-ε-CL) copolymer synthesized by ring-opening polymerization (ROP) have been used to reduce this negative effect. For the characterization of this copolymer, the Fourier Transform Infrared (FT-IR), Proton Nuclear Magnetic Resonance (1H-NMR), Differential Scanning Calorimetry (DSC) and Thermogravimetric Analysis (TGA) techniques were used. To investigate the impact of copolymer and salt (200 mM NaCl) singly and in combination, on two different sunflowers (Helianthus annuus L. cv. 'Düet' and Helianthus annuus L. cv. 'Meteor') a series of experiments were established. In the seedlings grown for 21 days, differences in some metabolic parameters such as chlorophyll-a (Chl-a), chlorophyll-b (Chl-b), carotenoids (Car), total chlorophyll (Chl), total carbohydrate content, and malondialdehyde (MDA) content were examined. According to the result of our study, after the application of NaCl and poly(EO-co-ε-CL), significant changes were observed in the studied biochemical parameters. It was observed that the copolymer in both species had a healing effect against salt stress. Application of 200 mM NaCl caused an increase in MDA of both genotype, particularly in 'Meteor'. Higher Chl-a, Chl-b, total Chl, and Car contents were observed in 'Meteor' as compared to that in 'Düet' under saline conditions. Total carbohydrate content decreased with increasing salt stress in both cultivars.

  AtıfScopus1

  İncele
• MakaleAçık Erişim2019/r/munzur/5213
  Pendimethalin stresine maruz kalan aspir (Carthamus tinctorius L.)’de oluşan bazı biyokimyasal değişiklikler

  Beker Akbulut, Gülçin

  Pendimethalin herbisiti tarımsal uygulamalarda yabancı otların kontrolü için yaygın olarak kullanılmaktadır. Bununla birlikte, pendimethalinin kültür bitkileri üzerindeki yan etkisi tam olarak bilinmemektedir. Bu çalışmada, aspir (Carthamus tinctorius L.) bitkisine ait Dinçer ve Remzibey çeşitlerinde pendimethalin kaynaklı oksidatif stres araştırıldı. Herbisit uygun büyüklüğe (21 günlük) gelen bitkilerin yapraklarına toksisite denemeleri sonrasında belirlenen dozlarda (0.004-0.01 M) çimlenme sonrası olarak uygulandı. Uygulama gruplarında karotenoid, toplam klorofil, toplam karbohidrat, toplam fenolik ve lipid peroksidasyonunu gösteren malondialdehid (MDA) içeriğindeki değişimler saptandı. Dinçer (% 42.7) ve Remzibey (% 59) çeşitlerinde karotenoid içeriği kontrole kıyasla azalış gösterdi. Toplam klorofil içeriği Remzibey çeşidinde (% 56.8) Dinçer’e (% 41.4) göre daha yüksek bulundu. Toplam karbohidrat içeriği Dinçer çeşidinde (% 50) Remzibey çeşidine (% 46.1) kıyasla daha yüksek saptandı. Toplam fenolik içeriği Remzibey (% 48.3) ve Dinçer (% 46.7) çeşitlerinde artan saatlere bağlı olarak azalış gösterdi. MDA içeriği Remzibey çeşidinde (% 72) Dinçer çeşidine (% 70) göre daha yüksek saptandı. Bu çalışma, pendimethalinin kültür bitkisi olan aspire toksik etki oluşturduğunu göstermiştir. 

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  AtıfOpenAlex3·TR Dizin3

  İncele
• MakaleYalnız künye2019Scopus SJRQ4/r/munzur/4869
  THE EFFECT OF PENDIMETHALIN HERBICIDE ON ANTIOXIDANT SYSTEM IN SAFFLOWER

  Beker-Akbulut, Gulcin

  Pendimethaline is a dinitroaniline herbicide compound which selectively controls weeds. It is a cell division and growth inhibitor. In this study, pendimethalin herbicide was applied to Dincer and Remzibey cultivars of Carthamus tinctorius L. in 0.004-0.01 M concentration range after germination. The effects of pendimethalin upon peroxidase (POD), ascorbate peroxidase (APX), superoxide dismutase (SOD), catalase (CAT) reduced glutathione (GSH), glutathione reductase (GR), glutathione-Stransferase (GST) and total phenolic content were investigated on the 24, 48 and 72 hours following the treatment. POD, APX, SOD, CAT and GST activities were increased on the 24, 48 and 72 hours in Dincer while decreased at 72 hour in Remzibey. GSH and GR activity were decreased at 72 hour in Dincer and Remzibey. Total phenolic content decreased at 48 and 72 hours in Dincer and Remzibey. In this study, we determined that pendimethalin herbicide affected the antioxidant enzyme activities in Dincer and Remzibey.

  AtıfWoS1

  İncele
• MakaleAçık Erişim2018Scopus SJRQ4/r/munzur/3885
  Effect of glyphosate on some protective systems in Zea mays L; [Glifosatın mısır (Zea mays L.)’da bazı koruyucu sistemlere etkisi]

  Beker Akbulut, Gulcin; Yigit, Emel; Bayram, Dilek

  Glyphosate is an herbicide that is applied after non-selective germination and affects plant growth. In this study, glyphosate was applied to Zea mays L. after germination, at a concentration range from 0.017 to 0.145 M in a growth chamber. The effects of this herbicide on some antioxidant enzymes, lipid peroxidation, total chlorophyll and total carbohydrate content were investigated on days 1, 5 and 10 following the application. Results showed that peroxidase (POD), ascorbate peroxidase (APX), superoxide dismutase (SOD), catalase (CAT) and glutathione-S-transferase (GST) were increased in the groups treated with glyphosate on days 5 and 10, but the activities of reduced glutathione (GSH) and glutathione reductase (GR) were decreased on day 10. Malondialdehyde (MDA) content indicating lipid peroxidation was increased on days 1, 5 and 10. Changes in total chlorophyll and total carbohydrate contents were found to be time-dependent. These increases and decreases in the antioxidant activities, MDA content, total chlorophyll and total carbohydrate content in Zea mays L. were determined as a symptom of cytotoxicity caused due to glyphosate.

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  AtıfScopus3·OpenAlex1

  İncele
• MakaleYalnız künye2018Scopus SJRQ1/r/munzur/3375
  Effects of salicylic acid and organic selenium on wheat (Triticum aestivum L.) exposed to fenoxaprop-p-ethyl

  Akbulut, Gulcin Beker; Yigit, Emel; Kaya, Armagan; Aktas, Aydın

  In this study, we evaluated effects of fenoxaprop-p-ethyl herbicide on wheat (Triticum aestivum L.). The wheat plants were grown in pots containing perlite. Fenoxaprop-p-ethyl was applied to adequately grown T. aestivum plants at determined doses (1 mM, 1.5 mM, 2.2 mM, 3.4 mM and 5.1 mM) via spraying. The effects of this herbicide on some antioxidant enzymes, lipid peroxidation, carotenoid, total chlorophyll and total phenolic were investigated on days 1, 5 and 10 following the application. Environmental pollutants cause oxidative stress, production of free radicals and changes in antioxidants. Synthesis of signal molecules such as salicylic acid (SA) is important for the stress response of plants. In addition to this, although selenium is known not to be an obligatory element for plants, low dose selenium has positive effects on the plant growth. We also investigated the effects that are elicited on parameters above mentioned by the treatment of exogenous 0.5 mM SA and 0.004 mM organic selenium (SeI) separately. Our findings reveal that SA and SeI, which are externally applied before the germination of seeds that are exposed to herbicide stress, becomes effective in reduction of the negative effects of fenoxaprop-p-ethyl, with regard to antioxidant system, lipid peroxidation, pigment system and total phenolic. When we evaluate the study results, our findings suggest that especially SA plays a protective role on wheat against herbicide stress.

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  AtıfScopus21·WoS19·OpenAlex24

  İncele
• MakaleYalnız künye2018Scopus SJRQ2/r/munzur/3098
  Effect of the novel biodegradable copolymer and soil salinity on the growth of corn plant

  Torğut, Gülben; Beker Akbulut, Gülcin

  Biodegradable and biocompatible poly (1,2-epoxy-3-phenoxy propane)-co-(ε-caprolactone) poly(EPP-co-ε-CL) copolymers were synthesized by ring-opening polymerization method at four different ε-CL ratios. Copolymers were characterized using Fourier-transform infrared spectroscopy, proton nuclear magnetic resonance (1H-NMR), thermogravimetric analysis, and differential scanning calorimetry. To examine their possible applications in agriculture, germination test and growth analyses of corn plants were carried out. For this purpose, corn (Zea mays L. cv. “72 May 80”) was allowed to germinate under suitable conditions. Copolymers, 200 mM salt (NaCl) solution, and both copolymers and salt solution were applied to the plants and compared with the control groups. On the 21st day, all the plants were harvested and were stored at −80°C until analysis. Differences in growth parameters, lipid peroxidation degrees, pigment analysis, and total carbohydrate analysis were tested in order to put forward copolymer and NaCl sensitivity of the corn. Statistical analysis was carried out using the SPSS 17.0 software program and the results were compared with each other. These results showed that the copolymers both have a positive effect on the carbohydrate and malondialdehyde (MDA), a product of lipid peroxidation content as well as the chlorophyll content. Moreover, poly(EPP-co-ε-CL0.88) was more efficient than was the other copolymers in the improvement of the detrimental effects of salinity in increasing chlorophyll, carbohydrate, and MDA content, and growth of corn plants. As a result of the study, it was demonstrated that all of the investigated parameters were affected by the applications of salt and copolymers.

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  AtıfScopus2·WoS2·OpenAlex3

  İncele
• MakaleYalnız künye2016Scopus SJRQ3/r/munzur/4211
  The effects of sebum configuration on Demodex spp. density

  Demirdag, Hatice Gamze; Özcan, Hamdi; Gürsoy, Sule; Beker Akbulut, Gulcin

  Background/aim: Demodex spp. are ectoparasites living in the pilosebaceous units, which feed on the host's sebum and cellular proteins. The protective barrier of the skin consists of sebum secretion, moisture, and the acid mantle. In this study, we aimed to determine the effects of skin sebum, moisture, pH levels, and sebum configuration on Demodex spp. density Materials and methods: Forty-five patients who had demodicosis were enrolled in the study group, while the control group consisted of 40 subjects without demodicosis. Body fat percentage, serum triglyceride and cholesterol levels, skin sebum, moisture, and pH levels were measured. Demodex spp. density was determined with a standardized skin surface biopsy. Sebum samples were taken from the forehead and a high-performance thin-layer chromatography (HPTLC) method was performed on these samples. Subsequently, densitometric analyses were applied to the HPTLC plates. Results: Demodex spp. were found on the cheeks and lived in an alkali environment. Skin sebum and moisture levels were low in all groups. The skin pH levels and cholesterol ester in the sebum configuration were determined to be significantly higher in the group with demodicosis. Conclusion: We suggest that Demodex spp. may use cholesterol ester in the sebum as nutriment. In other words, cholesterol ester may be a suitable growth medium for the proliferation of Demodex spp.

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  AtıfWoS23·OpenAlex28

  İncele
• MakaleYalnız künye2016Scopus SJRQ3/r/munzur/4107
  EFFECT OF SALICYLIC ACID AND SELENIUM ON ANTIOXIDANT SYSTEM OF AVENA SATIVA L. UNDER FENOXAPROP-P-ETHYL STRESS

  Yigit, Emel; Akbulut, Gulcin Beker; Bayram, Dilek; Kaya, Armagan; Gok, Yetkin

  The present study focuses on understanding the role of different antioxidants in plant defense against oxidative stress caused by fenoxaprop-p-ethyl herbicide. Besides ameliorative effect of exogenously applied salicylic acid (SA) and selenium (Se) on fenoxaprop-p-ethyl-induced toxicity was examined in Avena sativa L. It was determined that the MDA (malondialdehyde) content in the groups to which only herbicide was applied was higher than that of the groups to which SA and Se applied together with the herbicide. In plants exposed to fenoxaprop-p-ethyl, the activities of peroxidase (POD), ascorbate peroxidase (APX), superoxide dismutase (SOD), glutathione S-transferase (GST), glutathione reductase (GR) and content of glutathione (GSH) increased in the treatment groups compared to the control groups. The antioxidant activity increased in the control and treatment groups that were pre-treated with SA and Se compared to the groups that were not pre-treated with SA and Se. While catalase activity (CAT) decreased in SA pre-treated plants, it increased in Se pre-treated plants. These results indicate that the fenoxaprop-p-ethyl induces various plant defense responses in target plants and that treatment with exogenous SA and Se can increase stress resistance by altering these responses.

  AtıfWoS6

  İncele
• MakaleYalnız künye2014Scopus SJRQ3/r/munzur/4150
  THE EFFECTS OF REACTIVE PHTHALOCYANINE BLUE TEXTILE DYE ON PEROXIDASE ACTIVITY, LIPID PEROXIDATION AND TOTAL CHLOROPHYLL CONTENT OF PHASEOLUS VULGARIS L. CV. GINA

  Kaya, Armagan; Yigit, Emel; Akbulut, Gulcin Beker; Ates, Burhan

  In this study, the effects of Reactive Phthalocyanine Blue (RPB) changes on peroxidase, malondialdehyde (MDA) content and total chlorophyll content of Phaseolus vulgaris L. (bean) were examined. The beans were exposed to different concentrations of RPB between 200-1255 mg/L. In the analyzed plants, different effects were observed on peroxidase activity. When compared to the control group, a significant decrease was identified in the plants which were treated with RPB except for the 1255 mg/L application group. Besides the effects of RPB on MDA content were analyzed. MDA content was found to be lower in all application groups except for 439 mg/L. In addition, total chlorophyll content increased in all application groups except for 338 mg/L when compared to the control group. These findings indicated that RPB caused significant changes in peroxidase activity, MDA content and total chlorophyll. It was shown that the textile dye had a toxic effect on the plants.

  AtıfWoS2

  İncele