Sürdürülebilir Kalkınma Amaçları

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Araştırmacılar(8)

• Açar, Mikail
• AKBULUT, Gülçin BEKER
• Alataş, Mevlüt
• Aydın, Rahmi
• Kar, Bülent
• Taşar, Neslihan
• Torcan, Berat
• Yıldırım, Numan

• MakaleAçık Erişim2026/r/munzur/6088
  Pohlia wahlenbergii (Bryophyta)’nin Biyoaktif Potansiyeli: Antioksidan, Antidiyabetik ve Antimikrobiyal Aktiviteleri

  Alataş, Mevlüt; Erdem, Belgin; Çöteli, Ebru

  Briyofitler, biyolojik aktiviteleri yeterince incelenmemiş önemli bir bitki grubunu oluşturmaktadır. Yapılarındaki çok sayıda biyoaktif bileşik, onları önemli kılmaktadır. Bu çalışmada, Pohlia wahlenbergii karayosununun aseton ve metanol özütlerinin in vitro antioksidan, antidiyabetik ve antimikrobiyal aktiviteleri değerlendirilmiştir. Antioksidan aktivitenin belirlenmesinde, bu özütlerin iki farklı radikale, DPPH. ve ABTS.+'ya karşı inhibitör güçleri ölçülmüştür. Sonuçlar, standart askorbik asit sonuçlarıyla karşılaştırıldı. Ayrıca bu ekstraktların antidiyabetik aktivitesini belirlemek için, α-amilaz enzim inhibisyonu ölçüldü. Sonuçları karşılaştırmak için akarboz ilacı kullanıldı. En yüksek DPPH. ve ABTS.+ radikal temizleme aktivitesi sırasıyla 1.509 ve 1.301 mg/mL IC₅₀ değerleriyle aseton ekstraktında gözlendi. ABTS.⁺ radikal temizleme aktivitesinin daha güçlü olduğu bulundu. Antidiyabetik aktivitenin belirlenmesinde, α-amilaz enzim inhibisyonu kullanıldı ve metanol ekstraktında en yüksek seviyede olduğu görüldü (1.792 mg/mL). Ekstraktların antioksidan ve antidiyabetik aktivitelere sahip olduğu belirlendi. Ayrıca, Pohlia wahlenbergii'den elde edilen özütlerin çeşitli patojen mikroorganizmalara karşı in vitro antimikrobiyal potansiyeli değerlendirilmiştir. Sonuçlar, güçlü antifungal aktivite göstermiştir; aseton özütü, standart ilaç Flukonazol'den daha iyi performans göstererek Candida albicans'a karşı 25 mm'lik bir inhibisyon bölgesi ve 16 µg/mL'lik bir MIC sergilemiştir. Metanol özütü de geniş spektrumlu antibakteriyel etkiler göstermiş, özellikle Bacillus cereus ve Salmonella typhimurium'a karşı 20 mm'lik bir inhibisyon bölgesi göstermiştir. Pohlia wahlenbergii'nin seçici antimikrobiyal özellikleri, yeni doğal antibiyotikler ve antifungal ajanlar geliştirmek için potansiyel bir kaynak olduğunu göstermektedir.

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• MakaleAçık Erişim2026/r/munzur/5949
  Phylogenetic Analysis of Native Salmo Species in Türkiye Using Mitochondrial 12S rDNA, 16S rDNA, Cytochrome b Sequences, and Cytogenetics

  Yazdıç, Ferit Can; Kar, Bülent; Karaman, Altuğ

  In this study, phylogenetic relationships and genetic diversity of Salmo spp. populations in different water bodies in Türkiye were investigated, focusing on morphologically similar specimens to Salmo macrostigma. Significant genetic variability and evolutionary differentiation within these populations have been identified using mitochondrial regions sequences (12S rDNA, 16S rDNA, and Cyt-b), morphological observations, and cytogenetic analysis. Samples collected from eight river basins revealed three main phylogenetic clades and local adaptations influenced by ecological pressures. Cytogenetic findings showed that they have chromosome numbers ranging from 78 to 80 (2n), consistent with known Salmo species. For this purpose, three mitochondrial DNA fragments encoding 12S rDNA, 16S rDNA, and Cyt-b regions were amplified by PCR, sequenced, and then used for phylogenetic analysis of the studied species. The study revealed the need for comprehensive integrative taxonomic approaches to clarify the classification of Salmo spp. with critical implications for conservation strategies in Türkiye biodiversity hotspots. In conclusion, this study not only advances our knowledge of Salmo macrostigma or similar Salmo spp., but also suggests innovative approaches for future Salmonid phylogenetics and cytogenetics studies. The findings have important implications for understanding the evolution, taxonomy, and conservation of these economically and ecologically important fish.

  İncele
• MakaleAçık Erişim2026/r/munzur/5944
  Tunceli tarım arazilerinden elde edilen bacillus licheniformis'in antimikrobiyal özellikleri

  Yazdıç, Ferit Can; Kar, Bülent; Karaman, Altuğ; Yücel, Halit; Araka, Özlem

  Bu çalışmada, Türkiye Tunceli'deki tarım topraklarından izole edilen Bacillus licheniformis suşlarının antimikrobiyal potansiyelleri araştırılmıştır. Toprak örnekleri minimal olarak bozulmuş alanlardan toplanmış ve bakteri suşları morfolojik, biyokimyasal ve moleküler tanımlama yöntemleri kullanılarak izole edilmiştir. İzolatlar, 16S rDNA dizilemesi ve filogenetik analiz yoluyla Bacillus licheniformis oldukları başarılı bir şekilde doğrulanmıştır. Antimikrobiyal sekonder metabolitlerin üretimi, izolatların farklı karbon kaynaklarıyla (sükroz, glikoz ve fruktoz) kültürlenmesi ve disk difüzyon yöntemi kullanılarak Escherichia coli (ATCC 25922) ve Staphylococcus aureus'a (ATCC 25923) karşı aktivitelerinin test edilmesiyle değerlendirilmiştir. Sonuçlar, özellikle Escherichia coli'ye karşı antimikrobiyal aktiviteyi artırmak için en etkili karbon kaynağının sükroz olduğu belirlenmiştir. Antibakteriyel aktivite genellikle daha uzun inkübasyon süreleriyle artmıştır. Bu araştırma, Bacillus licheniformis'in antibakteriyel madde kaynağı olarak potansiyelini vurgulamaktadır. Patojenlerin biyolojik kontrolü için tarımdaki uygulamalarda kullanılması için çevre dostu bir alternatif sunmaktadır. Sonuç olarak, bu çalışmanın bulguları Bacillus licheniformis'in antimikrobiyal özellikleri hakkında bilgilere katkıda bulunmaktadır. Karbon kaynağındaki kullanılabilirliği ve metabolit üretimini etkileyen faktörleri anlamak, Bacillus licheniformis'in antimikrobiyal potansiyelinden yararlanmak için önemlidir.

  İncele
• MakaleAçık Erişim2026Scopus SJRQ1/r/munzur/1541
  A multi-evidence computational and experimental strategy for Silene nerimaniae: combining phytochemical characterization, bioactivity assessment, and ADEFRank-HCT-116-based metabolite prioritization

  Erharman, Ahmet; Yagi, Sakina; Uba, Abdullahi Ibrahim; Nikolić, Filip; Gašić, Uroš; Acar, Mikail; Dogan, Nurettin; Koyuncu, Ismail; Yapıcı, Ismail; Gulcin, Ilhami

  This study presents the first comprehensive evaluation of the phytochemical composition, antioxidant properties, enzyme inhibitory effects, and cytotoxic potential of Silene nerimaniae G.E.Genç, Kandemir & İ.Genç (family Caryophyllaceae). Ethyl acetate, acetone, 70% acetone, and aqueous extracts were obtained from the aerial parts of the plant through maceration. Among the tested extracts, the acetone extract contained the highest total phenolic (24.37 mg GAE per g) and flavonoid (24.64 mg RE per g) contents. LC–MS analysis identified 52 bioactive constituents, including sugars, organic acids, phenolic acids, flavonoids, and fatty acids. The aqueous extract demonstrated the strongest radical scavenging activity against DPPH (23.02 mg TE per g) and ABTS (55.76 mg TE per g), as well as the highest Fe3+ reducing power (37.53 mg TE per g). In combination with the acetone extract, it also exhibited notable Cu2+ reducing activity (47.78 mg TE per g). Regarding enzyme inhibition, the ethyl acetate, acetone, and hydroacetone extracts displayed the strongest inhibitory activities against acetylcholinesterase (1.87 mg GALA per g), butyrylcholinesterase (2.92 mg GALA per g), and tyrosinase (60.44 mg KAE per g), respectively. All three extracts also inhibited α-amylase activity within the range of 0.73–0.77 mmol ACAE per g (p ≥ 0.05). In addition, the hydroacetone extract showed considerable inhibition of human carbonic anhydrase isoenzymes I (IC50 = 38.78 μg mL−1) and II (IC50 = 19.59 μg mL−1). The ethyl acetate extract exhibited the strongest cytotoxic effect against the colorectal cancer cell line HCT-116, with an IC50 value of 0.639 μg mL−1 and a selectivity index of 1.7. To contextualize this HCT-116-associated activity at the metabolite level, an ADEFRank-HCT116 (abundance–domain–evidence fusion ranking) workflow was applied by integrating EtOAc abundance, HCT-116-oriented QSAR evidence, applicability-domain confidence, and structure-assignment reliability. This analysis prioritized diosmetin, luteolin, eriodyctiol, acacetin, and isorhamnetin as core candidates for structure-based follow-up. Molecular docking screening predicted favorable docking profiles for the prioritized candidates, while 100 ns molecular dynamics simulations were used to assess the dynamic stability of the representative luteolin–PI3Kα, diosmetin–AKT1, and isorhamnetin–GSK-3β complexes. Taken together, these results indicate that S. nerimaniae may represent a valuable source of active compounds for various functional nutraceutical, cosmetic and pharmaceutical uses. This journal is

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• MakaleYalnız künye2026Scopus SJRQ3/r/munzur/1511
  New national and regional bryophyte records, 86

  Wilbraham, J.; Abdirasulov, F.; Aguirre, J.; Alataş, M.; Álvaro Alba, W.R.; Aslan Ergenekon, Z.; Aswathy Raj, K.; Ayhan, E.; Batan, N.; Bednarek-Ochyra, H.

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• MakaleAçık Erişim2026Scopus SJRQ2/r/munzur/1461
  Antioxidant capacity and α-glucosidase inhibitor activity of Bryonia aspera extracts and its major constituent p-coumaric acid: Phytochemical analysis, biological evaluation and computational approach

  Tuncay, Salih; Karasakal, Ömer Faruk; Açar, Mikail; Toptancı, İsra; Şenol, Halil; Çırak, Olgun; Işık, Mesut; Beydemir, Şükrü

  This study comprehensively investigated the phenolic composition, antioxidant potential, and α-glucosidase inhibitory properties of B. aspera Steven ex Ledeb. extracts using integrated in vitro and in silico approaches. LC-MS/MS analysis identified seven major phenolic compounds, caffeic acid, chlorogenic acid, galangin, gallic acid, naringenin, p-coumaric acid, and rutin, with p-coumaric acid being the most abundant (306.71 mg/kg in aerial and 155.56 mg/kg in root extracts). Both extracts exhibited strong antioxidant and α-glucosidase inhibitory activities, particularly the aerial extract (IC50 = 0.678 and 0.173 for DPPH, ABTS, respectively and 0.022 mg/mL for α-glucosidase inhibition). Molecular docking and MM-GBSA analyses supported these findings, showing that p-coumaric acid had the strongest binding to α-glucosidase (–7.783 kcal/mol, ΔG = –47.76 kcal/mol), stabilized by key interactions with Asp-62, Arg-200, His-332, and Arg-400. A 250 ns molecular dynamics simulation confirmed the stability of the complex, and in silico ADME analysis revealed good oral absorption (67%) and compliance with Lipinski’s rule. Overall, results indicate that p-coumaric acid is the primary bioactive compound contributing to the antioxidant and α-glucosidase inhibitory activity of B. aspera, with promising potential as a natural lead for α-glucosidase inhibition.

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• MakaleAçık Erişim2026Scopus SJRQ2/r/munzur/1264
  Sustainable epoxy based biocomposites reinforced with Melilotus officinalis: Structural, mechanical, thermal, and dielectric performance evaluation

  Solmaz, Adnan; Açar, Mikail; Demirel, Maruf Hurşit; Deniz, Şermin; Aydoğmuş, Ercan

  The aim of this study is to investigate the manufacturability of Melilotus officinalis (L.) reinforced epoxy biocomposites as a renewable biofiller and to characterize the mechanical, thermal, and dielectric properties of these materials. For this purpose, composites containing biomass at 0, 3, 6, and 9 wt.% were prepared; the samples were examined by tensile testing, FTIR, SEM, EDX, XRD, Shore D hardness, thermal conductivity, and dielectric analysis. The findings showed that with increasing M. officinalis content, tensile strength, hardness, and density decreased, while ductility and elongation at break improved. The significant decrease in thermal conductivity revealed an increase in heat insulation efficiency. Dielectric analyses showed that strong interfacial polarization occurred at low frequencies, and the dielectric constant and loss values ​​decreased as the frequency increased. SEM examinations confirmed that the plant-derived filler was homogeneously distributed and formed acceptable interfacial adhesion with the matrix. In conclusion, although the use of synthetic epoxy matrix and the decrease in mechanical strength limit load-bearing structural applications, M. officinalis is a promising biofiller for lightweight and partially bio-based epoxy composites thanks to its increased ductility and improved thermal insulation properties. © The Author(s) 2026. This article is distributed under the terms of the Creative Commons Attribution-NonCommercial 4.0 License (https://creativecommons.org/licenses/by-nc/4.0/) which permits non-commercial use, reproduction and distribution of the work without further permission provided the original work is attributed as specified on the SAGE and Open Access pages (https://us.sagepub.com/en-us/nam/open-access-at-sage).

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  İncele
• MakaleAçık Erişim2026Scopus SJRQ2/r/munzur/1229
  Occurrence of Antimicrobial Resistance in Kocuria rhizophila Bacteria Isolated From Salmo munzuricus (Teleostei: Salmonidae) Samples in Natural Resources

  Kar, Bülent

  Global warming and environmental pollution trigger the emergence of antimicrobial-resistant (AMR) opportunistic pathogens in the aquatic microflora. This study aimed to determine the characterization and AMR profile of the opportunistic pathogen Kocuria rhizophila isolated from the tissues of Salmo munzuricus (Munzur trout), an endemic species. Samples collected from the Munzur River were identified using morphological and molecular (16S rDNA) methods; bacterial isolates were characterized by MALDI-TOF and sequencing. The resistance status against 11 different antibiotics was analyzed with the Kirby-Bauer method. The findings revealed that the isolates (with 99.51% accuracy as K. rhizophila) showed full resistance to Penicillin G (p < 0.01) and were moderately sensitive to gentamicin and tetracycline. High sensitivity to amoxicillin and ampicillin was detected. These findings demonstrate that even in natural environments, the fish microbiome in wild endemic populations can be susceptible to the development of AMR in response to environmental changes. Therefore, determining the resistance of opportunistic pathogens in wild fish species such as Salmo munzuricus to different classes of antibiotics and studying their spread is extremely important for natural ecosystems.

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• MakaleAçık Erişim2025/r/munzur/6163
  Molecular Insight on Phylogeny and Systematics of the Narrowly Endemic Centaurea bingoelensis (Asteraceae)

  Yazdıç, Ferit Can; Açar, Mıkaıl; Cakilcioglu, Ugur; Tasar, Neslihan; Çelik, İbrahim Seyfettin

  Centaurea L. is a genus that is systematically discussed and whose taxonomic issues need to be clarified in the light of molecular techniques developed in recent years. This study aims to contribute taxonomically in molecular terms by studying the morphological and phylogenetic relationships of Centaurea bingoelensis. This limited, endemic species grows naturally in the Bingöl province of Türkiye. A phylogenetic analysis based on the molecular marker ITS, which is the most preferred in evolutionary studies and found in nuclear DNA, has been proposed as a molecular marker of C. bingoelensis , an endemic plant in Türkiye, and has not yet been molecularly identified. For this purpose, PCR amplification of the fragment mentioned above was performed, followed by direct sequencing, analysis of nucleotide variation, and phylogenetic analysis with different species. The Centaurea species identified so far have been shown to have three main distinct major clades and the specific phylogenetic methods used (NJ, ML, MP) and the ITS nucleotide sequences analyzed revealed a close phylogenetic relationship between C. bingoelensis and C. spectabilis. For a more accurate phylogenetic classification of this species within the Asteraceae family, their close relationships were probed using similar sequences from the GenBank Database. Phylogenetic analysis of C. bingoelensis specimens using ITS sequences reveals their position in the Asteraceae family and molecular support for these specimens being new species as proposed. Although morphologically similar to C. fenzlii and C. obtusifolia, the remarkable point of our study is that C. bingoelensis is phylogenetically closer to C. spectabilis. This work has exciting implications for understanding the evolution and diversification of the genus Centaurea. In addition, the results of this study show that the samples identified as Centaurea species in Türkiye are still not entirely determined genetically.

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• MakaleAçık Erişim2025/r/munzur/6144
  The new locality records of Scorpidium cossonii and Cyrtomnium hymenophylloides (Bryophyta) for Türkiye and Southwest Asia from the Bolkar Mountains

  Alataş, Mevlüt; Batan, Nevzat; Uygur, Ahmet; Ezer, Tülay; Aslan, Züleyha

  The new locality records of Scorpidium cossonii and Cyrtomnium hymenophylloides (Bryophyta) for Türkiye and Southwest Asia from the Bolkar Mountains Tülay EZER 1, Ahmet UYGUR2, Züleyha ASLAN ERGENEKON3, Mevlüt ALATAŞ4, Nevzat BATAN5 ORCID: 0000-0002-6485-5505; 0000-0002-3934-8851; 0000-0002-5078-2317; 0000-0003-0862-0258; 0000-0001-8456-5719 1Niğde Ömer Halisdemir University, Faculty of Architecture, Department of Landscape Architecture, 51240, Niğde, Türkiye 2Aksaray University, Sabire Yazıcı Faculty of Science and Letters, Department of Biology, 68100, Aksaray, Türkiye 3Atatürk University, Faculty of Science, Department of Biology, 25240, Erzurum, Türkiye 4Munzur University, Vocational School of Tunceli, Department of Plant and Animal Production, 62000, Tunceli, Türkiye 5Karadeniz Technical University, Faculty of Science, Molecular Biology and Genetics, 61080, Trabzon, Türkiye Abstract During bryological land study to the Bolkar Mountains, wetland moss Scorpidium cossonii (Schimp.) Hedenäs, previously known only from Çanakkale, and the arctic-alpine moss Cyrtomnium hymenophylloides (Hübener) T.J.Kop., previously known only from Ordu province, were rediscovered in Türkiye. Scorpidium cossonii was collected on wet rocky bank near the Ciğer Lake (Çan-Çanakkale) in 2014. Cyrtomnium hymenophylloides was collected on wet soil in Kalıcak and Kadıncık Villages (Ulubey-Ordu) in 2014. Ten years later, both species were found the vicinity of Karagöl and Çiniligöl (Ulukışla-Niğde), glacial lakes located in the Central Anatolian part of the Bolkar Mountains. Scorpidium cossonii and Cyrtomnium hymenophylloides were recorded for the second time from Türkiye and Southwest Asia with the present study. While, Scorpidium cossonii is characterised by strongly curved non-plicate leaves and well developed hyalodermis, Cyrtomnium hymenophylloides is characterised erect tuft life form, broadly ovate leaves, acute to apiculate leaf apices. These new locality records will provide important information about the geographical distribution of the both species in Anatolia and will contribute to the bryophyte flora of Türkiye. Keywords: Bolkar Mountains, Central Anatolia, moss, new locality, Türkiye

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