Sürdürülebilir Kalkınma Amaçları

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• MakaleAçık Erişim2025/r/munzur/6210
  The Relationship between Copper Tolerance and Antibiotic Susceptibility of Vagococcus salmoninarum Isolated from Kılavuzlu Dam Lake

  Gokhan, Haci Bayram; Ispır, Ünal; Özcan, Mikail; Şeker, Engin

  The contamination of aquatic ecosystems by heavy metals has been identified as a significant environmental hazard for aquatic animal species, including fish. The objective of this study was to investigate the tolerance of Vagococcus salmoninarum bacteria to copper and the impact of copper exposure on antibiotic susceptibility. The bacterial isolates, comprising six strains, were obtained from rainbow trout reared in cage culture at Kılavuzlu Dam Lake. The initial assessment of antibiotic susceptibility was conducted using the Kirby-Bauer disc diffusion method, which was subsequently validated through the determination of minimum inhibitory concentration (MIC). The broth microdilution method was employed, with the bacterial isolates exposed to concentrations of copper sulphate ranging from 0.1 to 1 mM. The resistance to multiple antibiotics was altered to be significantly altered following exposure to copper sulphate. The minimum inhibitory concentration (MIC) value of copper was observed to be 0.4 mM against V. salmoninarum. It was noted that V. salmoninarum, which has been exposed to copper in its natural environment, has developed the capacity to withstand stress and has undergone a change in its physiological status.

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  İncele
• MakaleAçık Erişim2022/r/munzur/5820
  Immunomodulation function of Tunceli garlic (Allium tuncelianum) oil in Rainbow Trout (Oncorhynchus mykiss)

  Ispır, Ünal; Özcan, Mikail; Şeker, Engin

  The effects of injectable Tunceli garlic oil (Allium tuncelianum) on serum response of rainbow trout were examined. Influence of intraperitoneal (i.p.) injection administration of Tunceli garlic oil (TGO) was evaluated on some immunological factors of rainbow trout. Fish weight 40 g were i.p. injected TGO at doses of 1% and 10%. Serum lysozyme activity, bactericidal activity, myeloperoxidase, protein and immunoglobulin levels were measured on days 3, 7, 14 and 21 after the TGO administration. All the immune parameters assayed remained affected when TGO was i.p. administered. Serum bactericidal activity was consistently achieved by immunizing fish with TGO. Levels of serum myeloperoxidase activity were increased in the groups of fish injected with TGO. Myeloperoxidase and bactericidal activities peaked at 14 and 21 days post-injection, respectively. Lysozyme activity was significantly increased throughout experiment in the all groups. Total protein and immunoglobulin levels increased in the 21 days post-injection although no statistically significant differences. In conclusion, these results suggest that there are functionally effects of TGO on the immune mechanisms of rainbow trout.

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  AtıfOpenAlex2·TR Dizin1

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• MakaleYalnız künye2021/r/munzur/6669
  Changes in the Some Biochemical Constituents in Fry of Common Carp (Cyprinus carpio) Exposed to Imidacloprid

  Yonar, M. Enis; Ispır, Ünal; Özcan, Mikail; Şeker, Engin

  This study was conducted to investigate the possible toxic effects of imidacloprid biochemical parameters of carp. The fish (weight 0,34±0,03 g, total length 2,97±0,21 cm) were subjected to 2,8 and 5,6 mg L-1 of Imidacloprid concentration for 4 and 7 days. Alterations were observed in glycogene, total protein, and total fat activities in the whole body of carp. No mortalities occurred in any group during the test. The glycogene and total protein levels in imidacloprid treated fishes were significantly (p<0,05) lower than the control in all the concentrations. Total fat levels were higher in imidacloprid treatment groups, which were not significantly different. This study was conducted to investigate the possible toxic effects of imidacloprid biochemical parameters of carp.

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  AtıfOpenAlex2

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• MakaleAçık Erişim2012Scopus SJRQ2/r/munzur/4197
  Investigation of Yersinia ruckeri Infection in Rainbow Trout (Oncorhynchus mykiss Walbaum 1792) Farms by Polymerase Chain Reaction (PCR) and Bacteriological Culture

  Seker, Engin; Karahan, Murat; Ispir, Unal; Çetinkaya, Burhan; Saglam, Naim; Sarieyyüpoglu, Mustafa

  This study has been carried out to investigate the presence of Yersinia ruckeri, the causative agent of Enteric Red Mouth (ERM) disease, in rainbow trout fish breeding farms located in Elazig and Malatya provinces in Eastern Turkey. For this purpose, bacteriological culture and a specific polymerase chain reaction (PCR) have been compare with examine blood and intestine samples collected from adult and young rainbow trout fish as well as water samples collected from all the farms. Y. ruckeri was isolated and identified from eight of the 17 fish farms giving a proportion of 52.9% at fish farm. The disease was noted in five of the rainbow trout farms located in Elazig and three of the 11 rainbow trout farms located in Malatya. The agent was detected in five of the six young fish breeding farms. The isolation percentages of the causative agent at fish level were calculated as 25.7% (131/510) in adult and 31.7% (19/60) in young fish, giving an overall proportion of 26.3% (150/570). All the isolates have been successfully identified as Y ruckeri by species-specific PCR. No isolation could be made from water samples. It is therefore concluded that Y. ruckeri infection poses a significant threat to the fish farms in the study area and necessary precautions should urgently be taken in order to minimize economical losses due to ERM disease. A specific PCR can be utilized successfully in aquaculture for rapid identification of bacterial agents which will help to prevent spread of infectious diseases and will therefore contribute to the productivity of fishery sector.

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  AtıfWoS6·OpenAlex7

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