Sürdürülebilir Kalkınma Amaçları
Yayınlar
- MakaleAçık Erişim2026Scopus SJRQ2Change of micronucleus frequencies, antioxidant parameters and in silico in carp (Cyprinus carpio) fry exposed to ibuprofen
Kirici, Muammer; Tüzün, Burak; Yonar, Muhammet Enis; Poustforoosh, Alireza; Mişe Yonar, Serpil; Şeker, Engin; İspir, Ünal
Ibuprofen, a non-steroidal anti-inflammatory drug, has been detected in the aquatic environment, but little is known about its effects on fish and other aquatic organisms. This study aimed to investigate the effect of ibuprofen on micronucleus levels in erythrocytes and whole-body antioxidant parameters of carp (Cyprinus carpio) fry. Fish were exposed to immersion in two different doses of ibuprofen concentrations (50 and 250 μg/L) for 24, 48, 72 and 96 h and 14 days. No mortalities occurred in any group during the test. Abnormal behaviours such as irregular swimming, loss of balance and hanging vertically in the water were observed in some fish exposed to acute toxicity. In this study, a significant increase in the micronucleus number of red blood cells was observed throughout the study period (p < 0.05). Antioxidant parameters were measured spectrophotometrically. Results obtained showed that ibuprofen significantly increased (p < 0.05) malondialdehyde (MDA) levels, glutathione S-transferase (GST) and catalase (CAT) activities, but decreased (p < 0.05) the glutathione (GSH) levels of whole-body carp fry in a dose-dependent manner. Furthermore, ibuprofen significantly increased MDA, GST and CAT levels, but increased the GSH levels of whole-body carp fry in a time-dependent manner. These results indicate that carp fry is sensible to human pharmaceutical agents in aquatic environments. The manner and the constructed interactions between ibuprofen and two protein targets (immunoglobin and haemoglobin) were investigated using molecular docking and molecular dynamics simulation. The results indicated that ibuprofen could affect the haemoglobin activity. Finally, absorption, distribution, metabolism, excretion and toxicity analysis was performed to examine the drug potential of the studied molecules.
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İncele - MakaleAçık Erişim2025The Relationship between Copper Tolerance and Antibiotic Susceptibility of Vagococcus salmoninarum Isolated from Kılavuzlu Dam Lake
Gokhan, Haci Bayram; Ispır, Ünal; Özcan, Mikail; Şeker, Engin
The contamination of aquatic ecosystems by heavy metals has been identified as a significant environmental hazard for aquatic animal species, including fish. The objective of this study was to investigate the tolerance of Vagococcus salmoninarum bacteria to copper and the impact of copper exposure on antibiotic susceptibility. The bacterial isolates, comprising six strains, were obtained from rainbow trout reared in cage culture at Kılavuzlu Dam Lake. The initial assessment of antibiotic susceptibility was conducted using the Kirby-Bauer disc diffusion method, which was subsequently validated through the determination of minimum inhibitory concentration (MIC). The broth microdilution method was employed, with the bacterial isolates exposed to concentrations of copper sulphate ranging from 0.1 to 1 mM. The resistance to multiple antibiotics was altered to be significantly altered following exposure to copper sulphate. The minimum inhibitory concentration (MIC) value of copper was observed to be 0.4 mM against V. salmoninarum. It was noted that V. salmoninarum, which has been exposed to copper in its natural environment, has developed the capacity to withstand stress and has undergone a change in its physiological status.
- MakaleAçık Erişim2025Scopus SJRQ4Effects of chlorpyrifos on the freshwater mussel Unio elongatulus eucirrus (Bourguignat, 1860), with a focus on neurotoxicity and oxidative stress responses; [Efectos del clorpirifos en el mejillón de agua dulce Unio elongatulus eucirrus (Bourguignat, 1860), con especial atención a la neurotoxicidad y las respuestas al estrés oxidativo]
Zorlar, Faruk; Şeker, Engin; Pala, Ayşegül
This study set out to investigate the effects of the organophosphate insecticide chlorpyrifos (CPF) on certain biochemical parameters of the freshwater mussel Unio elongatulus eucirrus. Mussels were exposed to varying concentrations of CPF (10, 20, and 40 μg·L-1) for duration of 96 hours. Samples were collected from the mussels at the 24th and 96th hours of the experiment. The results indicated that exposure to CPF concentrations led to a decline in the activities of acetylcholinesterase (AChE), superoxide dismutase (SOD), catalase (CAT), and glutathione peroxidase (GPx) activities and, accompanied by an increase in malondialdehyde (MDA), and decreased reduced glutathione (GSH) levels in mussels. In conclusion, the present study demonstrates that CPF caused AChE inhibition, formation of oxidative stress, and negative effects on certain antioxidant parameters in the freshwater mussel U. elongatulus eucirrus.
- MakaleAçık Erişim2024Farklı Esansiyel Yağlarla Hazırlanmış Balık (Luciobarbus esocinus (Heckel, 1843)) Köftelerinin Mikrobiyolojik ve Duyusal Kalitesi
Kuzgun, Nermin Karaton; Şeker, Engin; Polat, Yunus
Bu çalışmada, nane (Spearmint sp.) ve kekik (Origanum spp.) esansiyel yağları ilave edilmiş Luciobarbus esocinus’dan elde edilen kıyma ile üretimi yapılan balık köftelerinin soğuk muhafaza esnasında mikrobiyolojik ve duyusal kalitesi araştırıldı. Nane, kekik ve kontrol grubu olmak üzere üç farklı deneysel köfte örneği 4±1°C’de muhafaza altına alınmış ve muhafazanın belirli günlerinde analizleri yapılmıştır. Deneysel köfte örneklerinin mikrobiyolojik olarak Toplam mezofilik aerob bakteri sayısı (TMAB), toplam psikrofil aerob bakteri sayısı (TPAB), toplam maya-küf sayısı, laktik asit bakteri sayısı ve koliform bakteri sayıları belirlenmiştir. Ayrıca çalışmada hazırlanan ürünlerin duyusal olarak değerlendirmeleri yapılırken görünüş, renk, koku ve lezzet bakımından incelenmiştir. Deneysel örneklerin muhafazasının son aşamasında toplam mezofilik aerobik bakteri sayısı 7,33±0,94 log kob/g (10. günde) ile kontrol grubunda, 7,03±1,43 log kob/g (15. günde) ile kekik esansiyel yağı ekli grupta, 7,81±1,29 log kob/g (20. günde) ile nane esansiyel yağı ekli grupta belirlenmiştir. Psikrofil bakteri sayısı balık köftesi örneklerinin depolama başlangıcında 4,04±1,52-4,72±0,54 log kob/g arasında ve toplam laktik asit bakterisi sayısı depolama başında 4,04±1,52- 4,72±0,54 log kob/g arasında olduğu belirlenmiştir. Depolama başlangıcındaki maya- küf sayısı 4,60±1,22, 4,25±1,22 ve 4,73±0,99 log kob/g (Sırasıyla Kontrol, nane ve kekik esansiyel yağı eklenmiş gruplarda) olarak belirlenmiştir. Duyusal değerlendirme bakımından muhafazanın ilk gününde en çok lezzet değeri 9,00±0,00 ile hem Ke hem de Na grubunda olduğu belirlenmiştir. Fakat duyusal genel olarak değerlendirildiğinde en yüksek puanların nane ekli grupta olduğu belirlenmiştir.
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İncele - MakaleAçık Erişim2023Karakaya Baraj Gölü’nde Yaşayan Arabibarbus grypus (Heckel, 1843) (Şabut) Populasyonunun Bazı Büyüme Özelliklerinin Belirlenmesi
Ozcan, Ebru Ifakat; Şeker, Engin; Cengiz, Burhan
Bu çalışmada; Karakaya Baraj Gölü’ndeki Arabibarbus grypus’un (Heckel, 1843) eşey dağılımı, boy ve ağırlık ilişkisi, yaş tayini, von Bertalanffy büyüme parametreleri ve kondisyon faktörü gibi bazı büyüme özellikleri araştırılmıştır. İncelenen 74 bireyin %37,84’ünün (28 adet) dişi, %62,16’sının (46 adet) erkek olduğu ve dişi/erkek oranının 1/0,6087 olduğu tespit edilmiştir. Örneklerin total boy ve ağırlıkları dişilerde 32,0-62,2 cm ve 354,0-3046 g; erkeklerde 25,0-63,0 cm ve 200,0-3350 g arasında değişmektedir. İncelenen örneklerin yaş grupları II ile VI arasında değişmektedir. Boy-ağırlık ilişkisi dişilerde W = 0,0067L3,079, erkeklerde W = 0,011L2,962 ve tüm bireylerde W = 0,0096L2,993 olarak hesaplanmıştır. Karakaya Baraj Gölü’ndeki A. grypus’un izometrik bir büyüme gösterdiği saptanmıştır (p>0,05). von Bertalanffy büyüme parametreleri dişiler için Lt = 76,53 [1 – e–0,348 (t + 1,22)], erkekler için Lt = 89,83 [1 – e–0,212 (t + 2,08)] ve tüm bireyler için Lt = 92,40 [1 – e–0,200 (t + 2,21)] olarak tahmin edilmiştir. Büyüme performans endeksi (Ф') değeri dişi, erkek ve tüm bireyler için sırasıyla 3,309; 3,233 ve 3,232 olarak hesaplanmıştır. Kondisyon faktörü dişi bireylerde 0,707-1,120 ve erkek bireylerde ise 0,702-1,356 arasında değişmektedir.
AtıfOpenAlex2TR Dizin2İncele - MakaleAçık Erişim2023Tunceli (Pertek ve Çemişgezek) ile Elazığ (Keban ve Sivrice) İlçelerinde Balık Tüketim Alışkanlıklarının Araştırılması ve Karşılaştırılması
Kuzgun, Nermin Karaton; Şeker, Engin; Kürşat, Muhammed
Bu araştırma, Elazığ ili Keban ve Sivrice ilçeleri ile Tunceli ilinin Pertek ve Çemişgezek ilçelerinin balık tüketim alışkınlıklarını tespit etmek için yapılmıştır. Bu araştırma, anket uygulaması ile yapılmıştır. Anketler, 2021 yılının Nisan-Haziran aylarında yüz yüze görüşme ve online anket metoduyla yapılmıştır. Araştırma sonuçlarına göre, bu çalışmanın katılımcıları en çok Pertek ilçesinden, en az Çemişgezek, ilçesinde bulunan katılımcılardan oluşmuştur. Ankete katılan katılımcıların en çok kesimi kırmızı eti, en az kesimi balıketini tercih ettikleri tespit edilmiştir. En fazla balık tüketimi, Pertek ve Keban ilinde olduğu gözlemlenmiştir.
AtıfOpenAlex2TR Dizin2İncele - MakaleAçık Erişim2022Immunomodulation function of Tunceli garlic (Allium tuncelianum) oil in Rainbow Trout (Oncorhynchus mykiss)
Ispır, Ünal; Özcan, Mikail; Şeker, Engin
The effects of injectable Tunceli garlic oil (Allium tuncelianum) on serum response of rainbow trout were examined. Influence of intraperitoneal (i.p.) injection administration of Tunceli garlic oil (TGO) was evaluated on some immunological factors of rainbow trout. Fish weight 40 g were i.p. injected TGO at doses of 1% and 10%. Serum lysozyme activity, bactericidal activity, myeloperoxidase, protein and immunoglobulin levels were measured on days 3, 7, 14 and 21 after the TGO administration. All the immune parameters assayed remained affected when TGO was i.p. administered. Serum bactericidal activity was consistently achieved by immunizing fish with TGO. Levels of serum myeloperoxidase activity were increased in the groups of fish injected with TGO. Myeloperoxidase and bactericidal activities peaked at 14 and 21 days post-injection, respectively. Lysozyme activity was significantly increased throughout experiment in the all groups. Total protein and immunoglobulin levels increased in the 21 days post-injection although no statistically significant differences. In conclusion, these results suggest that there are functionally effects of TGO on the immune mechanisms of rainbow trout.
AtıfOpenAlex2TR Dizin1İncele - MakaleYalnız künye2021Changes in the Some Biochemical Constituents in Fry of Common Carp (Cyprinus carpio) Exposed to Imidacloprid
Yonar, M. Enis; Ispır, Ünal; Özcan, Mikail; Şeker, Engin
This study was conducted to investigate the possible toxic effects of imidacloprid biochemical parameters of carp. The fish (weight 0,34±0,03 g, total length 2,97±0,21 cm) were subjected to 2,8 and 5,6 mg L-1 of Imidacloprid concentration for 4 and 7 days. Alterations were observed in glycogene, total protein, and total fat activities in the whole body of carp. No mortalities occurred in any group during the test. The glycogene and total protein levels in imidacloprid treated fishes were significantly (p<0,05) lower than the control in all the concentrations. Total fat levels were higher in imidacloprid treatment groups, which were not significantly different. This study was conducted to investigate the possible toxic effects of imidacloprid biochemical parameters of carp.
AtıfOpenAlex2İncele - MakaleYalnız künye2020Investigation of Endohelmints in The Fishes Hunted in Keban Dam Lake Çemişgezek Region (4th Region)
Şeker, Engin; Aktürk, Burcu; Pala, Ayşegül
This study was carried out on 210 fish, which were 52 Cyprinus carpio (Linnaeus, 1758), 49 Capoeta trutta (Heckel, 1843), 53 Lucio barbus mystaceus (Pallas, 1814) and 56 Lucio barbus esocinus (Heckel, 1843), which were hunted in the Keban Dam Lake Çemişgezek Region (4th Region) between July 2016 and December 2016. Three parasite species were encountered in 173 infested fish. After autopsy, two parasite types as Diplostomum sp. in the eyes fluid, Khawia sinensis in the intestine were identified in C. carpio. Two parasite species were identified in C. trutta, L. barbus esocinus and L. barbus mystaceus: Diplostomum sp. in eye fluids and Neoechinorhynchus rutili in their intestines. According to the results, it was determined that Diplostomum sp. was the most dominant parasite species with 73.33% prevalence and 8.97 density in 4 fish species examined. The prevalence rates of other identified parasite species N.rutili and K.sinensis were 40.95% and 15.38%, respectively, and densities were 7.11 and 2.25 respectively.
AtıfTR Dizin1İncele - MakaleAçık Erişim2018Scopus SJRQ3Effect of Tunceli garlic on some immunological parameters in Cyprinus carpio exposed to chlorpyrifos
Pala, Ayşegül; Şeker, Engin; Yonar, M. Enis
In this study, the effect of Tunceli garlic (Allium tuncelianium) on hemoglobin (Hb) level, oxidative radical production of neutrophils (Nitoblue tetrazolium assay-NBT activity) and total immunoglobulin (TI) content in carp (Cyprinus carpio) exposed to chlorpyrifos (CPF) was investigated. The 96 hour LC50 value of CPF on C. carpio was calculated to as 0.230 mg/L. The fishes were exposed to sublethal concentration of chlorpyrifos (1/8 of LC50 value: 0.029 mg/L), and Tunceli garlic (20 and 40 g/kg diet) was simultaneously administered for 21 days. Blood samples were taken from the fishes at 7, 14 and 21 days and analysed to determine the Hb levels, the NBT activity and the TI content. There was a significant decrease in the Hb level, the NBT activity and the TI content of CPF-treated fish. However, Tunceli garlic reversed the Hb level, the NBT activity and the TI content. In conclusion, this study demonstrated that CPF had a negative effect on the immunological values of the fish. The simultaneous administration of Tunceli garlic was neutralised CPF-induced toxicity.
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