Sürdürülebilir Kalkınma Amaçları
Yayınlar
- MakaleAçık Erişim2024Scopus SJRQ3Synthesis and characterization of chitosan-based pH-sensitive biofilm: An experimental design approach for release of vitamin B12
Onal, Ali; Demir, Hulya; Ince, Olcay Kaplan; Ince, Muharrem
Hydrogels because of their unique potentials, such as highwater content and hydrophilicity, are of interest for the controlled release of drug molecules. In the present study, a biofilm was produced using chitosan, a natural polymer. Characterization analyses of the synthesized biofilm were performed using Fourier-transform infrared spectroscopy (FT-IR), thermogravimetric analysis-differential thermal analysis, x-ray diffraction, and scanning electron microscopy-energy dispersive x-ray spectrometry. The analysis results of the film before loading were compared to the analysis made after adding vitamin B12 to the film. The release percentage of vitamin B12 was investigated by using ultraviolet-visible spectrophotometery. The FT-IR bands of vitamin B12 were in the range of 2,800-3,500 cm-1 and 1,000 cm-1-1,750 cm-1. When pH increased from 3 to 7, release of vitamin B12 from the biopolymer increased up to pH 5. While examining the release potential, parameters, such as pH, initial concentration of vitamin B12, release time, and solution volume, were optimized by using response surface methodology. It was determined that with the increase in release time and the initial concentration, pH contributed positively to the release of vitamin B12. The following conditions were determined for release of vitamin B12 from the biofilm: pH: 4.2, initial concentration of vitamin B12: 54.0 mg L-1, the release time: 117 min, and volume of solution: 2.1 mL. The analysis of variance results showed that the determination coefficients for the use of synthesized biofilm in vitamin B12 release were high and R2 =0.9704.
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İncele - MakaleAçık Erişim2023Scopus SJRQ4Physicochemical and Phytochemical Properties of Different Extracts of Sumac Plant (Rhus coriaria L.) Grown in Tunceli, Türkiye; [Tunceli'de Yetişen Sumak Bitkisinin (Rhus coriaria L.) Farklı Ekstraktlarının Fizikokimyasal ve Fitokimyasal Özellikleri]
Yuksel, Esra; Ince, Olcay Kaplan
In this study, sumac plant (Rhus coriaria L.) naturally grown in Tunceli (Türkiye) was collected from 5 different provinces and investigated for their phytochemical properties. In sumac samples, pH, color, ash amount, total phenolic content (TPC), 2, 2-diphenyl-1-picrylhydrazyl (DPPH) free radical scavenging capacity, metal chelating capacity, copper (II) ion reducing antioxidant capacity (CUPRAC), reducing power, mineral matter content, organic acids as tartaric acid, malic acid and citric acid, phenolic compounds as gallic acid, vanillic acid, caffeic acid, routine, resveratrol, (-)- epicatechin, and (+)- catechin hydrate contents were determined. The highest TPC was found in the acidified methanol extract (AME) and the DPPH free radical scavenging capacity was found in the acidified acetonitrile extract (AAE). It was determined that all extracts of sumac sample 5 (S5) collected from Pertek district, metal chelating capacity was higher than the other samples but lower than the metal chelating capacity of ethylene diamine tetra acetic acid (EDTA). CUPRAC was detected at the highest concentration of 118.0±3.0 mg caffeic acid equivalent kg-1 (mg CAE kg-1) in the AME, at the lowest 10.2±0.6 mg CAE kg-1 in the AAE for S5 sample. It was found that the reducing powers of all samples were found to be lower than the reducing power of synthetic antioxidants (butylhydroxytoluene (BHT), α tocopherol, and vitamin C), and the samples were rich in mineral substances, the predominant organic acid was malic acid, and phenolic compound was gallic acid.
AtıfScopus2OpenAlex2İncele - MakaleYalnız künye2022Enhancing motility of rainbow trout (Oncorhynchus mykiss) sperm by Tribulus terrestris extract supplementation
Kocabaş, Mehmet; Kutluyer Kocabaş, Filiz; Aksu, Önder; Karakavuk, Emrah; Ölçülü, Abdullatif; Ince, Olcay Kaplan
Tribulus terrestris (TT) is a famous traditional plant of family Zygophyllaceae and widely distributes around the world. TT has potential to elevate the testosterone, testosterone precursor and luteinizing hormone levels. In this study, trials were conducted to assessment the impact of Tribulus terrestris addition on sperm motility of rainbow trout (Oncorhynchus mykiss) for the first time. In the trial, we used to different concentrations [0 µg L-1 (Control), 200 µg L-1, 400 µg L-1, 600 µg L-1, 800 µg L-1 and 1000 µg L-1] of T. terrestris extract. Sperm motility characteristics and longevity were determined. In addition, protodioscin content of T. terrestris extract was assessed. The present study revealed that the presence of T. terrestris caused to increase in sperm motility. The increases in duration (49.00±3.61 s) and motility rate (91.67±2.89%) at 400 µg L-1 were statistically significant (p<0.05). Overall, addition of T. terrestris to activation solution can increase the sperm motility of rainbow trout.
- MakaleYalnız künye2022Comparison of Some Physical and Chemical Properties of Kefir Obtained from Different Kefir Cultures and Brands of Milk
Ince, Muharrem; Ince, Olcay Kaplan; Eryilmaz, Haydar
In this study, three different kefir cultures were used to ferment five different brands of milk samples. pH, the dry matter, ash amount, carbon dioxide (CO2) amount, titratable acidity, 1,1-diphenyl-2-picrylhydrazyl (DPPH) free radical scavenging capacity, the reducing power, the Fe(II) ions chelating capacity, the total phenolic content (TPC), copper(II) ion reductive antioxidant capacity (CUPRAC) and mineral matter contents were investigated in kefir samples and results were evaluated statistically. When kefirs’ CUPRAC, TPC, DPPH free radical scavenging capacity, Mg, Zn, and Na concentrations are evaluated in terms of kefir culture, KC1, KC2, KC3, KC1, KC1, and KC1, had the highest values (p<0.05), respectively. If it is evaluated in terms of different brands of milk, kefirs’ CUPRAC, TPC, DPPH free radical scavenging capacity and Na concentrations were found highest (p<0.05) which were produced by M5, M2, M1, and M4, respectively. The data obtained from the experimental studies it was determined that the kefir cultures and milk used had an effect on the quality of the kefir.
AtıfOpenAlex3TR Dizin2
İncele - MakaleAçık Erişim2022Scopus SJRQ3Adsorptive removal of nickel ions from wastewater by synthesized a novel biocomposite material and process optimization by response surface methodology
Ince, Muharrem; Ince, Olcay Kaplan; Alp, Hevidar
In the present study, a fixed-bed column study was performed using a novel biocomposite mate-rial, Amanita vaginata immobilized on Amberlite XAD-4 resin, to find out the adsorption behavior of nickel (Ni2+) ions from industrial wastewater. The influence of some important operating conditions including pH (3–7), biocomposite material amount (150–250 mg (XAD-4:Amanita vaginata, 5:1)) influent flow rate (4–8 mL min–1) besides Ni2+ ions initial concentration (20–40 mg L–1) on the uptake of Ni2+ solution were also optimized using a synthetic water solution. A central composite design combined with response surface methodology was utilized to maximize Ni2+ ions removal using A. vaginata immobilized on Amberlite XAD-4 resin as a biocomposite material in a column biosorption process. A mathematical model was derived from the central composite design approach for predicting responses and analysis of variance. Also, for checking model adequacy a lack of fit test was carried out. It was determined that the checked and proposed quadratic model was derived from the experimental design data. The biocomposite material adsorption capacity was calculated as 6.28 mg g–1. A high coefficient of determination (R2 = 0.9887) was presented by analysis of variance. Results confirmed that the amount of adsorbed Ni2+ amount did not change with the increase of the flow rate ratio and dose when the initial concentration of Ni2+ ions increased. The maximum adsorp-tion capacity of biocomposite material was achieved at 6.9 pH, 150.1 mg of biocomposite material amount, 7.8 mL min–1 of solution flow rate, and 39.7 mg L–1 of Ni2+ ions initial concentration. Finally, the developed method was applied to industrial wastewater to determine Ni2+ ions concentrations.
AtıfScopus4WoS4OpenAlex5İncele - MakaleYalnız künye2021Determination of Antioxidant Capacity, Phenolic and Elemental Composition in Syriac (Mardin) Wines by using Chromatographic and Spectrophotometric Methods
Sanyurek, Nesrin Karaca; Ince, Olcay Kaplan; Aydogdu, Burcu; Ince, Muharrem
Free radicals are responsible for many health problems, especially oxidative stress-related diseases. With conscious nutrition and the use of natural antioxidants, it is now possible to cope with these diseases or minimize their effects. The aim of this study was to determine pH, total acidity, total SO2, free SO2, relative SO2, dry matter, density, alcohol content, reducing sugar, ash, CUPRAC, DPPH free radical scavenging capacity, total phenolic content, elemental composition, and some phenolic compounds of Syriac (Mardin) wines. Estimated daily intake (EDI), target hazard quotients (THQ), total target hazard quotients (TTHQ), and relative risk (RR) were calculated, to assess a health risk resulting from regular consumption of Syriac wines. The elements EDI values through consumption of wines were less than the recommended dietary allowance values. Obtained THQ values were less than 1. When the obtained results are compared with the levels set by regulatory authorities, the investigated wine consumption does not pose a toxicological risk based on EDI, THQ, TTHQ, and RR values. In addition, this study showed that the Syriac wine’s antioxidant capacities are high and rich in phenolic compounds and elements.
AtıfScopus1WoS1OpenAlex3İncele - MakaleAçık Erişim2021Scopus SJRQ3A novel macrofungus for the biosorption of hazardous dye and its reuse potential: Experimental design approach using response surface methodology
Ince, Olcay Kaplan; Alp, Hevidar; Ince, Muharrem; Onal, Ali
In the present study, a novel biosorbent, namely Agaricus campestris, was used for the removal of Methylene Blue (MB) from an aqueous medium. The biosorbent was characterized using fourier-transform infrared spectrometer spectra, scanning electron microscopy-energy dispersive X-ray, and thermogravimetric analysis. The influence of important process parameters including pH (4–7), temperature (25°C–35°C), contact time (15–25 min), and biosorbent amount (40–80 mg) on the uptake of MB solution were also optimized, which significantly affected the removal efficiency of MB. A central composite design (CCD) combined with response surface modeling (RSM) was carried out to maximize MB removal by A. campestris for the adsorption process. A regression model was derived using RSM to predict the responses and analysis of variance (ANOVA), and to check model adequacy lack of fit test was used. The A. campestris adsorption capacity was determined as 24.2 mg g–1. The ANOVA showed a high coefficient of determination (R2 = 0.9987). This study clearly revealed that RSM was one of the most effective and appropriate method for the optimization of operating conditions. The possibility of reusing A. campestris was also investigated and, as a result, it was found quite possible and showed high performance in the recovery of MB.
AtıfScopus2WoS1OpenAlex2İncele - MakaleYalnız künye2020Scopus SJRQ2Evaluation the Weekly Intake of Some Wild Edible Indigenous Mushrooms Collected in Different Regions in Tunceli, Turkey
Alp, Hevidar; Ince, Muharrem; Ince, Olcay Kaplan; Onal, Ali
The quantity of some essential and non-essential elements of wild edible mushroom samples collected from Tunceli Province of Turkey was determined by using flame and electrothermal atomic absorption spectrometer after microwave digestion. The method accuracy was corrected using standard reference material (NIST SRM 1547-Peach Leaves). The essential element concentrations of analyzed mushroom samples were determined in the range of 0.036–0.563 mg kg−1 for calcium, 1.28–2.55 mg kg−1 for magnesium, 0.054–0.188 mg kg−1 for sodium, 1.00–4.57 mg kg−1 for copper, 212–480 mg kg−1 for iron, and 75–151 mg kg−1 for zinc but cobalt and chromium were not detected. Based on results, there were statistically significant differences between the element contents of analyzed mushroom species. Consequently, according to this study results, the weekly intake and target hazard quotient values of the elements show that the consumption of these mushrooms does not threaten human health. [Figure not available: see fulltext.].
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İncele - MakaleAçık Erişim2020Scopus SJRQ3Biosorptive removal of eriochrome black-T using agaricus campestris: Parameters optimization with response surface methodology
Alp, Hevidar; Ince, Muharrem; Ince, Olcay Kaplan; Onal, Ali
In this study, Eriochrome Black T (EBT) removal from aqueous media by Agaricus campestris (AC) which is an eco-friendly biosorbent was investigated using a central composite design (CCD) combined with response surface methodology. The CCD was chosen as the statistical prediction method to reduce the number of experiments which, in turn, will directly save time and chemicals. To opti-mize the process, various experimental independent variables such as the pH of the solution, contact time, adsorbent dosage and temperature were selected. The optimal experimental conditions were obtained as 6.5, 30°C, 20 min and 20 mg, for solution pH, temperature, contact time and adsorbent dosage, respectively. Under these optimized conditions, maximum EBT (100 mg L–1 initial concentra-tion) removal efficiency was calculated as 64.2 mg g–1. The independent variables and their interaction significance were checked using analysis of variance and it was found that the EBT biosorption on AC was highly significant (p < 0.001). The results were justified by the relatively high correlation coefficients (R2 = 0.9967) of the statistical prediction. The EBT biosorption was confirmed by fourier transform infrared spectroscopy (FTIR) analysis monitoring the functional groups during EBT biosorption on AC. This was the first report on the parameter optimization of EBT biosorp-tion using AC in an experimental design. The used biosorbent was characterized by FTIR, scanning electron microscope coupled with energy dispersive spectrometer and thermogravimetric analysis. The proposed biosorption process efficiently removed EBT, present in various aqueous mediums and can be used in industrial processes.
AtıfScopus17WoS12İncele - MakaleAçık Erişim2020Scopus SJRQ4Investigation of curcumin role on doxorubicin-induced tissue damage in terms of trace metal levels using etaas
Ince, Olcay Kaplan; Kandemir, Fatih Mehmet; Ince, Muharrem; Benzer, Fulya; Onal, Ali; Kucukler, Sefa
Doxorubicin (DXR) is one of the commonly used chemotherapeutic agents, which is highly toxic and causes gastrointestinal and cardiac toxicity. Curcumin is used as a spice and coloring agent in food, but also possesses anti-proliferating, potent antioxidant, antitumor-promoting and anti-carcinogenic properties in vitro and in vivo. In this study, the change in element concentrations in the tissues of healthy animals was examined by administering doxorubicin and curcumin separately, or both at the same time. For this purpose, 35 wistar albino male rats were selected and randomly divided into five groups of seven each. The objective of this study was to assess and compare the levels of copper, iron, zinc and selenium in the liver, kidney, heart, and testis of the control group and in the doxorubicin-induced tissues. According to the results, the Cu, Fe, Zn, Se amounts and the Cu/Fe, Cu/Zn, and Cu/Se ratios were present at different levels in each of the five groups. In terms of element concentration, the most affected tissue by doxorubicin application is the heart, and the least affected is the kidney. The ratios of Cu/Fe and Cu/Se in the liver and the heart were highest in Group II and Group III, respectively.
AtıfScopus2WoS2OpenAlex2İncele - MakaleYalnız künye2019Scopus SJRQ4Application of response surface methodological approach to optimize removal of Cr ions from industrial wastewater
Ince, Muharrem; Ince, Olcay Kaplan
In the present study, the adsorption of chromium (Cr) onto clay using response surface methodology (RSM) was investigated as an efficient approach for examining predictive model building and optimization. To optimize experimental variables and predictive regression models, statistical design models were carried out. A five-level four-fac-tor central composite design (CCD) combined with RSM was performed with synthetic aqueous solutions on natural clay. The experimental variables included pH and agitation speed and were selected for optimization. The Cr ions ideal removal conditions were obtained using pH 5.0, contact time 23.0 minutes, adsorbent dosage of 69.4 mg, and agitation speed of 135 rpm. Chromium removal efficiency was found at 23.16 mg g-1. The independent variables significance and their interactions were verified by means of the analysis of variance (ANOVA). These results were justified by the relatively high correlation coefficients (R2 = 0.9902) of the statistical prediction. Clay and Cr loaded clay were characterized by X-ray dif-fractometer (XRD) and energy dispersive spectroscopy (EDS). The proposed adsorption process efficiently removed the Cr ions present in various aqueous media and was also applied to various industrial wastewaters.
AtıfScopus5İncele - MakaleYalnız künye2018Scopus SJRQ3Response surface modeling for Pb(II) removal from alcoholic beverages using natural clay: Process optimization with box–behnken experimental design and determination by electrothermal AAS
Ince, Olcay Kaplan; Ince, Muharrem; Onal, Ali
A three-level four-factor Box–Behnken experimental design (BBD) combined with response surface modeling (RSM) was used for the optimization and modeling of Pb(II) adsorption from alcoholic beverages onto natural clay. The BBD method was chosen as the statistical prediction method to reduce the experimental numbers which saves time and chemicals and reduce analysis cost. Various independent process variables including solution pH (X1: 3.0– 7.0), contact time (X2: 2–60 min), adsorbent dosage (X3: 0.01-0.1 g), and agitation speed (X4: 50–150 rpm) were optimized. The optimal conditions used for Pb(II) removal were pH of 5.3, contact time 38.9 minutes, 0.071 g adsorbent dosage, and 98.7 rpm agitation speed, resulting in a maximum removal efficiency of 120 mg g-1. Pb was determined by electrothermal AAS. The significance of the independent variables and their interactions were tested by means of the analysis of variance (ANOVA) and are based on the ANOVA statistical value. The adsorption of Pb(II) onto clay was found to be highly significant with very low probability (p) values (<0.001). These results were justified by the relatively high correlation coefficients (R2 = 0.9965, R2 adj = 0.9930, and R2 Pred= 0.9819) of the statistical prediction. FTIR analysis confirmed major participation of amide, amines, ketones, including the primary alcohol groups, during Pb(II) biosorption. Clay was characterized by Fourier transform infrared spectroscopy (FTIR) and scanning electron microscope (SEM), coupled with energy dispersive X-Ray (EDX). The proposed adsorption process efficiently removes Pb(II) present in various aqueous media and can be used for industrial wastewater or drinks.
AtıfScopus6İncele - MakaleYalnız künye2017Scopus SJRQ1A food waste utilization study for removing lead(II) from drinks
Ince, Olcay Kaplan; Ince, Muharrem; Yonten, Vahap; Goksu, Ali
This is the first study about removal of lead (Pb(II)) from energy drinks. In this paper, food waste, namely eggshell (hydroxyapatite) utilization, was used to remove Pb(II) from mineral water and energy drinks. Mineral water and energy drinks were chosen for removal of lead since the latter is severely hazardous to human health even in small amounts. Response Surface Methodology (RSM) was performed to optimize the application process by practice of the quadratic model united with the Central Composite Design (CCD) and quadratic combined program was utilized to study the most effective parameters on aforementioned liquids. Through the application of variance analysis (ANOVA) factors critical to removing of lead were identified for each experimental design response. Maximum adsorption capacity of eggshell was achieved as 923 mg g(-1) for Pb(II). The obtained optimum conditions were applied to drinks. Results showed that used adsorbent was quite effective in removing Pb(II) from drinks. (C) 2016 Elsevier Ltd. All rights reserved.
AtıfWoS27OpenAlex28İncele - DüzeltmeYalnız künye2017Scopus SJRQ1A food waste utilization study for removing lead(II) from drinks (vol 214, pg 637, 2017)
Ince, Olcay Kaplan; Ince, Muharrem; Yonten, Vahap; Goksu, Ali
- MakaleYalnız künye2017Scopus SJRQ3Box-Behnken design approach for optimizing removal of copper from wastewater using a novel and green adsorbent
Ince, Muharrem; Ince, Olcay Kaplan
The aim of the present research was to apply the Box-Behnken experimental design and response surface methodology for modeling of copper (Cu2+) ions from an industrial wastewater and leachate pretreated astragalus herbal plant. A three factor, three level Box-Behnken experimental design combined with response surface modeling (RSM) and quadratic programming (QP) was employed for maximizing Cu(ID removal from aqueous solution based on 17 different experimental data obtained in a batch experimental study. Three independent variables (pH ranging from 2.0 to 8.0, contact time from 10 to 180 minutes, and adsorbent amount from 0.1 to 1 g) were studied. The significance of the independent variables and their interactions were tested by means of the analysis of variance (ANOVA) with 95% confidence limits. The standardized effects of the independent variables and their interactions on the dependent variable were also investigated by preparing a Pareto chart. The optimum values of the selected variables were obtained by solving the quadratic regression model as well as by analyzing the response surface contour plots. Using the experimental conditions of pH = 5.0, contact time = 92.5 minutes, adsorbent dosage = 0.25 g, the recovery efficiency of Cu(II) was found to be 100%. Copper-free and copperloaded astragalus was analyzed using fourier transform infrared spectrometry (FTIR) which evidenced the functional groups involved in copper uptake. Adsorption equilibrium for copper was described by using the Freundlich and Langmiur adsorption isotherms. This adsorbent was applied to different industrial wastewater and leachate samples for copper removal.
AtıfScopus17İncele - MakaleYalnız künye2017Scopus SJRQ3Using food waste biomass as effective adsorbents in water and wastewater treatment for Cu(II) removal
Ince, Muharrem; Ince, Olcay Kaplan; Asam, Eren; Önal, Ali
The present study investigated Cu(II) ion adsorption onto banana peel, chestnut shell, and tea leaf waste in aqueous medium under different experimental conditions. Batch adsorption studies were carried out with various Cu(II) concentrations as well as the influence of process parameters such as pH, contact time, and adsorbent amount on the removal of copper, and the optimum conditions for maximum removal of copper from an aqueous solution. These food waste biomass adsorbents were applied to mineral water and industrial wastewater samples and the copper removal potentials were compared. The nature of the adsorbents and metal ion interactions was examined by the fourier transform infrared spectroscopy (FTIR) technique. The adsorption data were obtained by using the Langmuir model showing maximum adsorption capacity of banana peel, chestnut shell, and tea leaf waste at 1.94 mg g-1 2.25 mg g-1, and 3.36 mg g-1, respectively. Copper was determined by flame atomic absorption spectrometry.
AtıfScopus19İncele - MakaleYalnız künye2016Scopus SJRQ3Optimization of Cadmium Removal from Water by Hydroxyapatite Using Experimental Design Methodology
Ince, Olcay Kaplan; Ince, Muharrem; Karaaslan, Nagihan M.; Yonten, Vahap
The presence of cadmium in water is a serious threat to the environment. The removal of cadmium using hydroxyapatite as an adsorbent was studied in an aqueous system. Hydroxyapatite was prepared to process the adsorption of cadmium from aqueous solution. Experimental design methodology and response surface methodology (RSM) with a quadratic model associated with central composite design was used to optimize the pH, initial heavy metal concentration, final volume, and adsorbent dosage for the removal of cadmium. Analysis of variance identified significant factors on each parameter. RSM indicated that a pH of 8.5, a sample volume of 166 mL, an adsorbent of dosage 0.57 g, and a contact time of 82 min were optimal for the adsorption of Cd(II). Under these conditions, the removal of Cd(II) was 895 mg g(-1).
AtıfWoS17OpenAlex15İncele - MakaleAçık Erişim2016Scopus SJRQ2Nickel, Lead, and Cadmium Removal Using a Low-Cost Adsorbent - Banana Peel
Ince, Muharrem; Ince, Olcay Kaplan; Yonten, Vahap; Karaaslan, Nagihan M.
Water pollution because of inorganic contaminants is a serious problem due to the acute toxicities and carcinogenic nature of pollutants. Recently, much attention has been focused on using natural and low-cost adsorbents, especially food residues, for removing toxic metal ions. Batch removal of nickel (Ni(II)), lead (Pb(II)), and cadmium (Cd(II)) from aqueous solution using a natural adsorbent is described in this study. Effects of various parameters on adsorption capacities such as pH, adsorbent dose, and stirring time were investigated by batch experiments. The maximum adsorption capacities for Ni(II), Pb(II), and Cd(II) ions were 1.6 mg g(-1), 125 mg g(-1), and 84 mg g(-1), respectively. Characterization of the surface modification was performed with FTIR measurements. FTIR spectra showed that chemisorption takes place at the surface of the adsorbent. The Freundlich and Langmuir adsorption isotherms were used to evaluate the equilibrium data. The characteristic parameters for each isotherm were determined. The Langmuir and Freundlich isotherm provided the best correlation for Ni(II), Cd(II), and Pb(II) using banana peel.
AtıfWoS10OpenAlex13İncele - MakaleYalnız künye2012Scopus SJRQ2ANTIOXIDANT PROPERTIES OF WILD EDIBLE MUSHROOM PLEUROTUS ERYNGII COLLECTED FROM TUNCELI PROVINCE OF TURKEY
Yildirim, Nuran Cikcikoglu; Turkoglu, Semra; Yildirim, Numan; Ince, Olcay Kaplan
Antioxidants are our first line of defense against free radical damage, and are critical for maintaining health. The need for antioxidants becomes even more critical with increased exposure to free radicals. In the past few years there has been an increasing interest in determining relevant dietary sources of antioxidant phenolics. Mushrooms have been not only used as food materials with their unique flavor and texture, but also recognized as an important source of biologically active compound of medicinal value. In our study, to determine the antioxidant properties of the methanolic extracts of Pleurotus eryngii strains collected from different region of Tunceli, radical scavenging activity, total phenolic contents, reducing power, metal chelating activity and total antioxidant status (TAS) were analyzed. The analysis of phenolic compounds was performed by HPLC. In particular, the methanolic extract of P. eryngii collected from city center revealed the highest DPPH radical scavenging activity and reducing power, while the highest total phenolics and total antioxidant status was determined in P. eryngii collected from Pulumur. P. eryngii collected from Ovacik showed the highest chelating effects. Concentration of phenolic compounds found in three wild edible mushrooms. Kaempferol and catechin were not determined in musrooms species. The highest rutin levels were determined in ovacik. Resveratrol was determined only in Pulumur. The studied wild mushrooms might be beneficial to protect human body against oxidative damage.
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