Sürdürülebilir Kalkınma Amaçları
Yayınlar
- MakaleAçık Erişim2026Scopus SJRQ1Ellagic Acid Mitigates Lead (Pb)-Induced Toxicity in Cyprinus carpio: A Multi-Biomarker Assessment of Hematological, Immunological, and Oxidative Stress Responses Supported by Molecular Docking
Eroglu, Mucahit; Cakmak, Mehmet Nuri; Pala, Aysegul; Uslu, Harun; Yonar, Serpil Mise; Ispir, Unal; Orhan, Cemal; Yonar, Muhammet Enis
Lead (Pb) is a widespread environmental pollutant that induces systemic toxicity in aquatic organisms primarily through oxidative stress, hematological disruption, and immune dysfunction. This study investigated the protective effects of ellagic acid (EA) against Pb-induced toxicity in common carp (Cyprinus carpio) using a multi-biomarker approach and molecular docking. Fish were assigned to six experimental groups: control, EA-treated, Pb-I, Pb-I + EA, Pb-II, and Pb-II + EA. Fish in the Pb-I and Pb-II groups were exposed to 2.5 and 5 mg/L Pb, respectively, while EA was administered via diet at 100 mg/kg for 14 days. At the end of the exposure period, hematological indices, innate immune parameters, and oxidative stress biomarkers were assessed in blood, liver, kidney, and gill tissues. Pb exposure caused marked hematological impairment, suppressed immune responses, increased malondialdehyde levels, and disrupted antioxidant defense by reducing SOD, CAT, GSH-Px, and GSH levels while increasing GST activity. In contrast, dietary EA supplementation significantly mitigated Pb-induced alterations, improved hematological and immunological responses, reduced lipid peroxidation, restored antioxidant capacity, and normalized GST activity to control levels. Molecular docking analyses further showed that EA interacts with hemoglobin and immunoglobulin M, supporting its potential role in preserving oxygen transport and immune functions under Pb-induced stress. Overall, the findings demonstrate that Pb-induced toxicity involves coordinated disruption of redox homeostasis and immune function, whereas EA exerts a multi-target protective effect through biochemical and molecular mechanisms. This study provides mechanistic insight into chemical-biological interactions and supports the potential application of natural bioactive compounds in mitigating heavy metal-induced toxicity.
- MakaleYalnız künye2015Scopus SJRQ3ANTIOXIDANT RESPONSES OF RAINBOW TROUT (Oncorhynchus mykiss) GILLS AFTER EXPOSURE TO HYDROGEN PEROXIDE
Seker, Engin; Ispir, Unal; Yonar, Serpil Mise; Yonar, M. Enis; Turk, Cebrahil
We evaluated antioxidant responses of Oncorhynchus mykiss, experimentally exposed to different concentrations of hidrogen peroxide (H2O2). Different concentrations of H2O2 (50 and 100 ppm) were administrated to the fish for 30, 60 and 90 min. Samples of the gill were collected at the end of the experiment, and analysed for their oxidant antioxidant status, including the malondialdehyde (MDA) level, the catalase (CAT) and glutathione peroxidase (GSH-Px) activity as well as the reduced glutathione (GSH) concentration. Results obtained showed that H2O2 significantly increased MDA level. Compared to controls, GSH level and GSH-Px and CAT activities were significantly reduced in the fish that were exposed to different concentrations of H2O2. The current data suggest that H2O2 has an oxidative-stress-inducing potential in fish. The various parameters studied in this investigation can also be used as biomarkers of exposure to H2O2.
AtıfWoS18
İncele - MakaleAçık Erişim2012Scopus SJRQ2Investigation of Yersinia ruckeri Infection in Rainbow Trout (Oncorhynchus mykiss Walbaum 1792) Farms by Polymerase Chain Reaction (PCR) and Bacteriological Culture
Seker, Engin; Karahan, Murat; Ispir, Unal; Çetinkaya, Burhan; Saglam, Naim; Sarieyyüpoglu, Mustafa
This study has been carried out to investigate the presence of Yersinia ruckeri, the causative agent of Enteric Red Mouth (ERM) disease, in rainbow trout fish breeding farms located in Elazig and Malatya provinces in Eastern Turkey. For this purpose, bacteriological culture and a specific polymerase chain reaction (PCR) have been compare with examine blood and intestine samples collected from adult and young rainbow trout fish as well as water samples collected from all the farms. Y. ruckeri was isolated and identified from eight of the 17 fish farms giving a proportion of 52.9% at fish farm. The disease was noted in five of the rainbow trout farms located in Elazig and three of the 11 rainbow trout farms located in Malatya. The agent was detected in five of the six young fish breeding farms. The isolation percentages of the causative agent at fish level were calculated as 25.7% (131/510) in adult and 31.7% (19/60) in young fish, giving an overall proportion of 26.3% (150/570). All the isolates have been successfully identified as Y ruckeri by species-specific PCR. No isolation could be made from water samples. It is therefore concluded that Y. ruckeri infection poses a significant threat to the fish farms in the study area and necessary precautions should urgently be taken in order to minimize economical losses due to ERM disease. A specific PCR can be utilized successfully in aquaculture for rapid identification of bacterial agents which will help to prevent spread of infectious diseases and will therefore contribute to the productivity of fishery sector.
AtıfWoS6OpenAlex7İncele - MakaleYalnız künye2011Scopus SJRQ2Immunostimulating Effect of Levamisole on Spleen and Head-Kidney Leucocytes of Rainbow trout (Oncorhynchus mykiss, Walbaum 1792)
Seker, Engin; Ispir, Unal; Dörücü, Mustafa
An in vitro cultivation method for spleen and head kidney organ section were used to investigate the effects of levamisole on the immune response. After 10 days of culture with 10 mu g/ml, 50 mu g/ml and 100 mu g/ml or no levamisole in the culture media, the non specific defense reaction were measured by glass-adherent NBT (nitroblue tetrazolium) positive cells activation and phagocytic activity. Elevation in phagocytic activity was found for 10 mu g/ml and 50 mu g/ml levels of levamisole (P < 0.001), but no differences were found at levels of phagocytic activity in between 100 mu g/ml levamisole groups and control groups (P > 0.05). In the spleen and head kidney leucocytes, increased response to levamisole was shawn for all doses in the glass-adherent NBT positive cells activation.
AtıfWoS7İncele
