Sürdürülebilir Kalkınma Amaçları
Yayınlar
- MakaleYalnız künye2026Evaluation of the Effects of Chrysin in Rats Developed with Lead Acetate-Induced Neurotoxicity Using Biochemical, Histopathological, and Immunohistochemical Methods
Kandemir, Fatih Mehmet; Benzer, Fulya; Küçükler, Sefa; Yildirim, Serkan; Kandemir, Özge; Dalkılınç, Elif; Uçar, Aybüke Gözde Gültekin
Lead (Pb) is a heavy metal with high toxicity that can cross the blood-brain barrier and accumulate in brain tissue, causing serious neurological disorders. The aim of this study is to evaluate the potential protective effect of chrysin (CHR) against Pb-induced neurotoxicity. Thirty-five male Sprague Dawley rats were divided into five groups: Control, CHR (50 mg/kg), Pb, Pb+CHR (25 mg/kg), and Pb+CHR (50 mg/kg). Rats were administered CHR (25 or 50 mg/kg) and Pb (30 mg/kg) orally for 7 days. Twenty-four hours after the last treatment, the rats were sacrificed under mild sevoflurane anesthesia by decapitation, and brain tissue samples were collected. Biochemical and histopathological methods were used to measure cholinergic enzyme activity, DNA damage, and oxidative stress parameters in brain tissue. CHR reduced levels of glial fibrillary acidic protein (GFAP), acetylcholinesterase (AChE), and 8-hydroxy-2'-deoxyguanosine (8-OHdG) caused by Pb. Due to its antioxidant properties, CHR decreased Pb-induced lipid peroxidation while increasing glutathione (GSH) levels and the activities of catalase (CAT), superoxide dismutase (SOD), and glutathione peroxidase (GPx). Based on biochemical and immunohistochemical analyses, CHR reduced cyclooxygenase-2 (COX-2), myeloperoxidase (MPO), nuclear factor kappa-B (NF-κB), neuronal nitric oxide synthase (nNOS), prostaglandin E2 (PGE-2), and p53 levels. These findings suggest that CHR may exert a therapeutic effect by alleviating oxidative stress and inflammation induced by Pb-induced neurotoxicity.
- MakaleAçık Erişim2021Investigation of Chrysin Effects on Lead Asetat-Induced Liver Damage in Rats
Benzer, Fulya; Kandemir, Fatih Mehmet; Yildirim, Serkan; Coşkun, Pınar; Küçükler, Sefa
This study was conducted to investigate the effects of chrysin (CH) on lead acetate (PbA) inducing liver injury. In this study, 35 male Sprague Dawley rats were divided into 5 groups as 7 rats in each group. Group 1 (Control): Saline was given orally. Group 2 (CH group): CH (50 mg kg-1 weight day-1 ) were orally given rats for 7 days. Group 3 (PbA group): PbA (30 mg kg-1 ) was orally given to rats at 30 mg kg-1 PbA for 7 days. Group 4 (PbA +CH 25 group): 25 mg/kg CH was orally given to rats for 7 days. In addition, 30 mg kg-1 PbA was given orally to rats for 7 days after 30 minutes of the application of the CH. Group 5 (PbA+CH 50 group): 50 mg kg-1 CH was orally given to rats for 7 days. Furthermore, 30 mg kg-1 PbA was given orally to rats for 7 days after 30 minutes of the application of the CH. The rats were euthanized under mild sevoflurane anesthesia and tissue and blood samples were taken 24 hours after the last application (8th day). AST, ALT and ALP in serum, MDA, GSH and NO levels and SOD, CAT, GPx and arginase enzyme activities in liver tissue were measured. Serum ALT, AST and ALP levels increased in the PbA group when compared with the control, and this increase was partially corrected with CH. Liver MDA and NO levels increased in the PbA administered groups while antioxidant enzyme activities and GSH levels decreased. Although, CH administration decreased the increased MDA and NO levels, it increased the decreased antioxidant enzyme activities and GSH levels. Histologically, it was determined that CH decreased the necrosis, hydropic degenerations and mononuclear cell infiltrations seen in the livers of rats treated with PbA. As a result, CH is effective in the reducing PbA damages in the liver.
AtıfOpenAlex3TR Dizin3
İncele - MakaleYalnız künye2019Scopus SJRQ3Palliative effect of curcumin on doxorubicin-induced testicular damage in male rats
Aksu, Emrah Hicazi; Kandemir, Fatih Mehmet; Yıldırım, Serkan; Küçükler, Sefa; Dörtbudak, Muhammed Baheeddin; Çağlayan, Cüneyt; Benzer, Fulya
This study aimed to investigate the effect of curcumin (CUR) on doxorubicin (DOX)-induced testicular damage in male rats. Thirty-five adult male Wistar rats were used. Control group was received saline for 7 days. CUR group received CUR for 7 days. DOX group received single dose DOX on the 5th day. DOX+ CUR-100 group received 100 mg/kg/day CUR for 7 days and DOX injection on the 5th day. DOX + CUR-200 group received 200 mg/kg/day CUR for 7 days and DOX injection on the 5th day. DOX treatment decreased in sperm motility rate, live sperm percentages, cellular antioxidants, and increased malondialdehyde (MDA) levels, necrosis, degenerations, and slimming in seminiferous tubules, and DNA damages in testes by inducing oxidative stress. CUR treatment mitigated significantly these side effects when compared with DOX group in a dose-dependent manner. In conclusion, CUR treatment can be used in the mitigation of DOX-induced testicular toxicity.
AtıfScopus56WoS51OpenAlex68
İncele