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- MakaleYalnız künye2026Evaluation of the Effects of Chrysin in Rats Developed with Lead Acetate-Induced Neurotoxicity Using Biochemical, Histopathological, and Immunohistochemical Methods
Kandemir, Fatih Mehmet; Benzer, Fulya; Küçükler, Sefa; Yildirim, Serkan; Kandemir, Özge; Dalkılınç, Elif; Uçar, Aybüke Gözde Gültekin
Lead (Pb) is a heavy metal with high toxicity that can cross the blood-brain barrier and accumulate in brain tissue, causing serious neurological disorders. The aim of this study is to evaluate the potential protective effect of chrysin (CHR) against Pb-induced neurotoxicity. Thirty-five male Sprague Dawley rats were divided into five groups: Control, CHR (50 mg/kg), Pb, Pb+CHR (25 mg/kg), and Pb+CHR (50 mg/kg). Rats were administered CHR (25 or 50 mg/kg) and Pb (30 mg/kg) orally for 7 days. Twenty-four hours after the last treatment, the rats were sacrificed under mild sevoflurane anesthesia by decapitation, and brain tissue samples were collected. Biochemical and histopathological methods were used to measure cholinergic enzyme activity, DNA damage, and oxidative stress parameters in brain tissue. CHR reduced levels of glial fibrillary acidic protein (GFAP), acetylcholinesterase (AChE), and 8-hydroxy-2'-deoxyguanosine (8-OHdG) caused by Pb. Due to its antioxidant properties, CHR decreased Pb-induced lipid peroxidation while increasing glutathione (GSH) levels and the activities of catalase (CAT), superoxide dismutase (SOD), and glutathione peroxidase (GPx). Based on biochemical and immunohistochemical analyses, CHR reduced cyclooxygenase-2 (COX-2), myeloperoxidase (MPO), nuclear factor kappa-B (NF-κB), neuronal nitric oxide synthase (nNOS), prostaglandin E2 (PGE-2), and p53 levels. These findings suggest that CHR may exert a therapeutic effect by alleviating oxidative stress and inflammation induced by Pb-induced neurotoxicity.
- MakaleAçık Erişim2021Investigation of Chrysin Effects on Lead Asetat-Induced Liver Damage in Rats
Benzer, Fulya; Kandemir, Fatih Mehmet; Yildirim, Serkan; Coşkun, Pınar; Küçükler, Sefa
This study was conducted to investigate the effects of chrysin (CH) on lead acetate (PbA) inducing liver injury. In this study, 35 male Sprague Dawley rats were divided into 5 groups as 7 rats in each group. Group 1 (Control): Saline was given orally. Group 2 (CH group): CH (50 mg kg-1 weight day-1 ) were orally given rats for 7 days. Group 3 (PbA group): PbA (30 mg kg-1 ) was orally given to rats at 30 mg kg-1 PbA for 7 days. Group 4 (PbA +CH 25 group): 25 mg/kg CH was orally given to rats for 7 days. In addition, 30 mg kg-1 PbA was given orally to rats for 7 days after 30 minutes of the application of the CH. Group 5 (PbA+CH 50 group): 50 mg kg-1 CH was orally given to rats for 7 days. Furthermore, 30 mg kg-1 PbA was given orally to rats for 7 days after 30 minutes of the application of the CH. The rats were euthanized under mild sevoflurane anesthesia and tissue and blood samples were taken 24 hours after the last application (8th day). AST, ALT and ALP in serum, MDA, GSH and NO levels and SOD, CAT, GPx and arginase enzyme activities in liver tissue were measured. Serum ALT, AST and ALP levels increased in the PbA group when compared with the control, and this increase was partially corrected with CH. Liver MDA and NO levels increased in the PbA administered groups while antioxidant enzyme activities and GSH levels decreased. Although, CH administration decreased the increased MDA and NO levels, it increased the decreased antioxidant enzyme activities and GSH levels. Histologically, it was determined that CH decreased the necrosis, hydropic degenerations and mononuclear cell infiltrations seen in the livers of rats treated with PbA. As a result, CH is effective in the reducing PbA damages in the liver.
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İncele - MakaleYalnız künye2021Scopus SJRQ2Chrysin protects against testicular toxicity caused by lead acetate in rats with its antioxidant, anti-inflammatory, and antiapoptotic properties
Ileriturk, Mustafa; Benzer, Fulya; Aksu, Emrah Hicazi; Yildirim, Serkan; Kandemir, Fatih Mehmet; Dogan, Tuba; Dortbudak, Muhammet Bahaeddin; Genc, Aydin
In the present study, the protective effects of chrysin (CHR) against testicular damage caused by lead acetate (PbAc) were examined. In this way, 30 min after rats were given 25 and 50 mg/kg/b.w CHR orally for seven consecutive days, 30 mg/kg/b.w PbAc was administered orally. In biochemical analysis of testicular tissue, it was found that PbAc-reduced antioxidant parameters [glutathione peroxidase (GPx), glutathione (GSH), superoxide dismutase (SOD), and catalase (CAT)], while it increased lipid peroxidation, inflammatory markers [nuclear factor kappa-B (NF-κB), cyclooxygenase-2 (COX-2), prostaglandin E2 (PGE-2), and inducible nitric oxide synthase (iNOS)], and 8-hydroxy-2′-deoxyguanosine (8-OHdG). In the immunohistochemical examination, it was determined that PbAc increased the expression of tumor necrosis factor-α (TNF-α) and caspase-3. Accordingly, PbAc was found to cause a decrease in sperm motility and an increase in the percentage of dead sperm. However, it has been observed that CHR relieves oxidative stress due to its antioxidant properties, thus protecting against inflammation and apoptosis. It also allowed the CHR sperm parameters to return to control group levels. The results revealed that CHR could be a natural substance to be used in Pb-induced testicular toxicity. Practical applications: Lead (Pb) is an important environmental contaminant heavy metal. Pb is believed to reduce fertility in men. Oxidative stress plays a significant role in the damage caused by Pb to testicular tissue. CHR is an antioxidant substance that occurs naturally in various plants and has various pharmacological properties. In the present study, it was investigated whether CHR has a protective effect against testicular toxicity induced by PbAc. The results revealed that in rats, CHR protects the testicular tissue from PbAc toxicity by showing antioxidant, anti-inflammatory and anti-apoptotic effects, thus bringing sperm parameters closer to normal.
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İncele - MakaleYalnız künye2021Scopus SJRQ1Protective Effects of Chrysin Against Oxidative Stress and Inflammation Induced by Lead Acetate in Rat Kidneys: a Biochemical and Histopathological Approach
Kucukler, Sefa; Benzer, Fulya; Yildirim, Serkan; Gur, Cihan; Kandemir, Fatih Mehmet; Bengu, Aydin Sukru; Ayna, Adnan; Caglayan, Cuneyt; Dortbudak, Muhammet Bahaeddin
In this study, the protective effects of chrysin (CR) on lead acetate (PbAc)-induced renal toxicity in Sprague-Dawley rats were investigated with biochemical, histopathological, and immunohistochemical methods. In the study, rats were given orally at 30 mg/kg/body weight (BW) PbAc after CR of 25 and 50 mg/kg/BW was administered to them orally (a total of 7 administrations for 7 days). The results showed that CR reduced urea and creatinine levels by alleviating PbAc-induced kidney damage. It was determined that CR decreases PbAc-induced lipid peroxidation due to its antioxidant properties and increases catalase (CAT), superoxide dismutase (SOD), glutathione peroxidase (GPx) activities, and glutathione (GSH) levels. It was also detected that CR protects DNA from the toxic effects of PbAc and reduces 8-hydroxy-2′-deoxyguanosine (8-OHdG) levels. Biochemical and immunohistochemical findings demonstrated that CR had anti-inflammatory and antiapoptotic effects and reduced nuclear factor kappa-B (NF-κB), interleukin-33 (IL-33), prostaglandin-E2 (PGE-2), tumor necrosis factor-α (TNF-α), p53 levels, and the activities of cyclooxygenase-2 (COX-2) and inducible nitric oxide synthase (iNOS), which were increased with PbAc administration. Moreover, CR was found to increase the levels of aquaporin-1 (AQP-1) and nephrine in PbAc-induced kidney tissue. CR decreased the contents of lead (Pb), zinc (Zn), iron (Fe), sodium (Na), and copper (Cu) and increased those of potassium (K) calcium (Ca) in renal tissue. These results indicated that CR considerably alleviates kidney toxicity caused by PbAc.
AtıfScopus136WoS118OpenAlex145İncele - MakaleAçık Erişim2020Scopus SJRQ2Influence of vitamin E and vitamin E-selenium combination on arginase activity, nitric oxide level and some spermatological properties in ram semen
Ozer Kaya, Seyma; Gur, Seyfettin; Erisir, Mine; Kandemir, Fatih Mehmet; Benzer, Fulya; Kaya, Emre; Turk, Gaffari; Sonmez, Mustafa
The effects of vitamin E and vitamin E-selenium combination on seminal plasma arginase activity and nitric oxide level and some spermatological properties in rams were investigated in this study. For control group, animals were injected intramuscularly with physiological saline. For vitamin E group, rams were injected intramuscularly with 300 mg/ram vitamin E. For vitamin E + selenium group, animals were injected intramuscularly with 5 ml/ram vitamin E + selenium. The semen was collected by artificial vagina at 1st, 4th, 24th, 48th and 72nd hr after administration in each group. Significant decreases in seminal plasma arginase activity (at 1st, 24th and 48th hr), nitric oxide level (at 72nd hr) and abnormal sperm rate (at 1st, 24th and 72nd hr), and significant increases in semen volume (at 24th hr), semen mass activity (at 24th and 48th hr), sperm motility (at 24th, 48th and 72nd hr) and concentration (at 1st hr) were observed in vitamin E group compared with control group. Similarly, significant increase in semen volume (at 1st, 24th and 48th hr), mass activity, (at 48th hr), motility (at 48th and 72nd hr) and concentration (at 4th, 24th and 48th hr), and significant decrements in abnormal sperm rate (at 1st, 24th, 48th and 72nd hr), seminal plasma nitric oxide level (at 1st, 4th, 24th and 48th hr) and semen pH (at 24th and 48th hr) were detected in vitamin E + selenium group in comparison to the control group. As a result, it is suggested that vitamin E and/or vitamin E + selenium applications may improve reproductive performance.
AtıfScopus19WoS16OpenAlex19İncele - MakaleAçık Erişim2020Scopus SJRQ4Investigation of curcumin role on doxorubicin-induced tissue damage in terms of trace metal levels using etaas
Ince, Olcay Kaplan; Kandemir, Fatih Mehmet; Ince, Muharrem; Benzer, Fulya; Onal, Ali; Kucukler, Sefa
Doxorubicin (DXR) is one of the commonly used chemotherapeutic agents, which is highly toxic and causes gastrointestinal and cardiac toxicity. Curcumin is used as a spice and coloring agent in food, but also possesses anti-proliferating, potent antioxidant, antitumor-promoting and anti-carcinogenic properties in vitro and in vivo. In this study, the change in element concentrations in the tissues of healthy animals was examined by administering doxorubicin and curcumin separately, or both at the same time. For this purpose, 35 wistar albino male rats were selected and randomly divided into five groups of seven each. The objective of this study was to assess and compare the levels of copper, iron, zinc and selenium in the liver, kidney, heart, and testis of the control group and in the doxorubicin-induced tissues. According to the results, the Cu, Fe, Zn, Se amounts and the Cu/Fe, Cu/Zn, and Cu/Se ratios were present at different levels in each of the five groups. In terms of element concentration, the most affected tissue by doxorubicin application is the heart, and the least affected is the kidney. The ratios of Cu/Fe and Cu/Se in the liver and the heart were highest in Group II and Group III, respectively.
AtıfScopus2WoS2OpenAlex2İncele - MakaleAçık Erişim2020Scopus SJRQ2The changes in semen quality, arginase activity and nitric oxide level in dexamethasone-treated rams
Ozer Kaya, Seyma; Erisir, Mine; Gur, Seyfettin; Kandemir, Fatih Mehmet; Benzer, Fulya; Kaya, Emre; Sonmez, Mustafa; Turk, Gaffari
This study was made to investigate the effects of intramuscular administrations of dexamethasone on seminal plasma nitric oxide levels and arginase activity, and some spermatological parameters in rams. Ten Akkaraman rams weighing 50–60 kg and 2 years old were used as material in this study. The study was performed during the breeding season (September–November) for rams. The semen was collected by artificial vagina at 1st, 4th, 24th, 48th, 72nd and 96th hours for control group before dexamethasone administration. For treatment group, 0.25 mg/kg dexamethasone was administered and semen was collected at the time points described for control group. Spermatological characteristics of semen samples (semen volume, pH, sperm motility, density and abnormal sperm rate), seminal plasma arginase enzyme activities and nitric oxide levels were determined. It was determined that the administration of dexamethasone was detected to decrease seminal plasma arginase activity (p <.05 and.01) and nitric oxide level (p <.05), semen volume (p <.05 and.01), mass activity (p <.05 and.01), sperm density (p <.05) and sperm motility (p <.05 and.01), and to increase abnormal sperm rate (p <.05 and.01). In conclusion, dexamethasone is not recommended to be used during the breeding season as it damages the sperm quality of the rams.
AtıfScopus10WoS7OpenAlex8İncele - MakaleAçık Erişim2020Scopus SJRQ2Evaluation of the role of L-arginine on spermatological parameters, seminal plasma nitric oxide levels and arginase enzyme activities in rams
Ozer Kaya, Seyma; Kandemir, Fatih Mehmet; Gur, Seyfettin; Erisir, Mine; Benzer, Fulya; Kaya, Emre; Turk, Gaffari; Sonmez, Mustafa
The purpose of this study is to investigate the effects of L-arginine on spermatological parameters, seminal plasma nitric oxide levels and arginase enzyme activities. Fertile rams that are 2–3 years old and weighing 50–60 kg were used as material. The semen was collected by artificial vagina at 1st, 4th, 24th, 48th, 72nd, 96th and 120th hours for the control group before L-arginine administration. For treatment groups, L-arginine was administered intraperitoneally at a dose of 5 mg kg−1 bw−1 and semen was collected at the time point described for the control group. Spermatological characteristics of semen samples (semen volume, pH, sperm motility, concentration and abnormal sperm rate), seminal plasma nitric oxide levels and arginase enzyme activities were determined. Increased seminal plasma nitric oxide level (p <.01), seminal plasma arginase activity (p <.01), semen volume (p <.05), semen mass activity (p <.05), sperm motility (p <.05) and concentration (p <.01) and decreased abnormal sperm rate (p <.05 and p <.01) were observed by L-arginine administration. In conclusion, it may be concluded that L-arginine application in rams during the breeding season may have positive effects on rams' reproductive performance.
AtıfScopus14WoS10OpenAlex11İncele - MakaleYalnız künye2019Scopus SJRQ3Palliative effect of curcumin on doxorubicin-induced testicular damage in male rats
Aksu, Emrah Hicazi; Kandemir, Fatih Mehmet; Yıldırım, Serkan; Küçükler, Sefa; Dörtbudak, Muhammed Baheeddin; Çağlayan, Cüneyt; Benzer, Fulya
This study aimed to investigate the effect of curcumin (CUR) on doxorubicin (DOX)-induced testicular damage in male rats. Thirty-five adult male Wistar rats were used. Control group was received saline for 7 days. CUR group received CUR for 7 days. DOX group received single dose DOX on the 5th day. DOX+ CUR-100 group received 100 mg/kg/day CUR for 7 days and DOX injection on the 5th day. DOX + CUR-200 group received 200 mg/kg/day CUR for 7 days and DOX injection on the 5th day. DOX treatment decreased in sperm motility rate, live sperm percentages, cellular antioxidants, and increased malondialdehyde (MDA) levels, necrosis, degenerations, and slimming in seminiferous tubules, and DNA damages in testes by inducing oxidative stress. CUR treatment mitigated significantly these side effects when compared with DOX group in a dose-dependent manner. In conclusion, CUR treatment can be used in the mitigation of DOX-induced testicular toxicity.
AtıfScopus56WoS51OpenAlex68İncele - MakaleYalnız künye2018Scopus SJRQ2Chemoprotective effects of curcumin on doxorubicin-induced nephrotoxicity in wistar rats: by modulating inflammatory cytokines, apoptosis, oxidative stress and oxidative DNA damage
Benzer, Fulya; Kandemir, Fatih Mehmet; Kucukler, Sefa; Comaklı, Selim; Caglayan, Cuneyt
Doxorubicin (DXR) is one of the most important chemotherapeutic agent. However, nephrotoxicity reduces its clinical utility in humans. The aim of the study was to investigate protective effects of curcumin (CMN) against DXR-induced nephrotoxicity. Rats were subjected to oral treatment of CMN (100 and 200 mg/kg body weight) for 7 days. Nephrotoxicity was induced by single intra peritoneal injection of DXR (40 mg/kg body weight) on the fifth day and then the experiment was terminated on the eighth day. Nephroprotective effects of CMN were associated with decrease in serum toxicity markers and increase in antioxidant enzyme activities. CMN was able to reduced the levels of inflammatory markers such as TNF-α, NF-κB, IL-1β, iNOS and COX-2 in the rats. It also reduced the expressions of apoptotic marker including caspase-3, and oxidative DNA damage marker including 8-OHdG. Collectively, these findings indicated that CMN protect against DXR-induced nephrotoxicity.
AtıfScopus116WoS111OpenAlex122İncele - MakaleYalnız künye2018Scopus SJRQ3Curcumin ameliorates doxorubicin-induced cardiotoxicity by abrogation of inflammation, apoptosis, oxidative DNA damage, and protein oxidation in rats
Benzer, Fulya; Kandemir, Fatih Mehmet; Ozkaraca, Mustafa; Kucukler, Sefa; Caglayan, Cuneyt
Doxorubicin (DXR) is a highly effective drug for chemotherapy. However, cardiotoxicity reduces its clinical utility in humans. The present study aimed to assess the ameliorative effect of curcumin against DXR-induced cardiotoxicity in rats. Rats were subjected to oral treatment of curcumin (100 and 200 mg/kg body weight) for 7 days. Cardiotoxicity was induced by single intraperitoneal injection of DXR (40 mg/kg body weight) on the 5th day and the rats sacrificed on 8th day. Curcumin ameliorated DXR-induced lipid peroxidation, glutathione depletion, decrease in antioxidant (superoxide dismutase, catalase, and glutathione peroxidase) enzyme activities, and cardiac toxicity markers (CK-MB, LDH, and cTn-I). Curcumin also attenuated activities of Caspase-3, cyclooxygenase-2, inducible nitric oxide synthase, and levels of nuclear factor kappa-B, tumor necrosis factor-α, and interleukin-1β, and cardiac tissue damages that were induced by DXR. Moreover, curcumin decreased the expression of 8-OHdG and 3,3′-dityrosine. This study demonstrated that curcumin has a multi-cardioprotective effect due to its antioxidant, anti-inflammatory, and antiapoptotic properties.
AtıfScopus154WoS143OpenAlex163İncele - MakaleAçık Erişim2015Scopus SJRQ2Rutin attenuates gentamicin-induced renal damage by reducing oxidative stress, inflammation, apoptosis, and autophagy in rats
Kandemir, Fatih Mehmet; Ozkaraca, Mustafa; Yildirim, Betul Apaydin; Hanedan, Basak; Kirbas, Akin; Kilic, Kubra; Aktas, Esra; Benzer, Fulya
Gentamicin is commonly used against gram-negative microorganisms. Its therapeutic use is mainly limited by nephrotoxicity. This study was aimed at evaluating the effect of rutin on oxidative stress, inflammation, apoptosis, and autophagy in gentamicin-induced nephrotoxicity in rats. The rats were treated with saline intraperitoneally (group I), 150 mg/kg of rutin orally (group II), 80 mg/kg of gentamicin intraperitoneally for 8 d (group III), or 150 mg/kg of rutin plus 80 mg/kg of gentamicin (group IV). The serum urea, creatinine, kidney malondialdehyde (MDA), and reduced glutathione (GSH) levels and superoxide dismutase (SOD), catalase (CAT), and glutathione peroxidase (GPx) activity and protein concentration were measured, and renal histopathology analysis and immunohistochemical staining were performed. Rutin pretreatment attenuated nephrotoxicity induced by gentamicin by reducing the urea, creatinine, and MDA levels and increasing the SOD, CAT, and GPx activity, and the GSH levels. The rutin also inhibited inducible nitric oxide synthase (iNOS), cleaved caspase-3 and light chain 3B (LC3B), as evidenced by immunohistochemical staining. The present study demonstrates that rutin exhibits antioxidant, anti-inflammatory, anti-apoptotic, and anti-autophagic effects and that it attenuates gentamicin-induced nephrotoxicity in rats.
AtıfWoS107OpenAlex123İncele - MakaleYalnız künye2014Sığırların normal doğum ve güç doğumunda eritrosit arginaz aktivitesi ve plazma nitrik oksit düzeylerinin karşılaştırılması
Kandemir, Fatih Mehmet; Yüksel, Murat; Benzer, Fulya; Erişir, Mine; Hanedan, Başak
This study was aimed to investigate erythrocyte arginase activity and plasma nitric oxide (NO)levels in dystocia and normal calving of cows and compare these parameters. The study materialconsisted of 16 cows, 3 8 years old, brought for parturition at term to the Clinic of Obstetrics andGynecology at F ı rat University. Blood samples of cows normally calving and dystocia were takeninto blood collecting tubes with heparin from the jugular vein in the first half hour after delivery andcentrifuged at 3000 rpm for 10 minutes. Plasma NO levels and erythrocyte arginase activities weremeasured. The erythrocyte arginase activity in cows normally calving was significantly increasedcompared to that in cows with dystocia (P<0.01), and the plasma NO level in cows normally calvingwas significantly decreased compared to that in cows with dystocia (P<0.05). It was concluded thatthe lower erythrocyte arginase activity and higher plasma NO level in cows might play a role indystocia.
- MakaleAçık Erişim2012Scopus SJRQ2Chemotherapeutic Agent-induced Nephrotoxicity in Rabbits: Protective Role of Grape Seed Extract
Benzer, Fulya; Kandemir, Fatih Mehmet; Ceribasi, Songul; Ozkaraca, Mustafa; Yildirim, Nuran Cikcikoglu; Ozan, Sema Temizer
Cisplatin (CP) is one of the most effective cancer chemotherapeutic agent used against various solid tumors. This study was designed to investigate the antioxidant effects of Grape Seed Extract (GSE) on CP induced oxidative damage in rabbit kidney. The material of study was consist of 18 male New Zealand rabbit. Animals were divided into 3 groups. The first group was administered a single intraperitoneal (i.p.) close of 0.9% saline. The second group of rabbits was treated with CP (a single i.p. dose of 5 mg kg(-1) body weight); The third group of rabbits was treated with GSE by gavage at the dose of 250 mg kg(-1) body weight for 6 consecutive days before and 6 consecutive days after a single i.p. CP injection. As a result of CP administration, kidney function tests; urea, BUN (Blood Urea Nitrogen) and creatinine levels were increased (p<0.001). It was observed that CP increase malondialdehide (MDA) levels, the most important indicator of oxidative damage and decreased the activity of catalasc (CAT). Administration of CP did not caused statically a change in glutathione (GSH) levels and glutathione peroxidase (GSH-P-x) activity (p>0.05). GSE treatment has high levels of MDA to compared to normal levels (p<0.001) but not an increase in CAT (p<0.05). In CP treatment group, severe acute tubular necrosis was seen. It was determined that in CP+GSE group mild degenerative changes were received instead of acute tubular necrosis. GSE has been found to be partially protective effect against CP induced biochemical and histopathological changes in rabbit kidney.
AtıfWoS7OpenAlex7İncele - MakaleYalnız künye2011Scopus SJRQ2BENEFICIAL EFFECTS OF GRAPE SEED EXTRACT AGAINST CISPLATIN-INDUCED TESTICULER DAMAGE IN RABBITS
Yildirim, Nuran Cikcikoglu; Kandemir, Fatih Mehmet; Benzer, Fulya
Biological compounds with antioxidant properties may contribute to the protection of cells and tissues against deleterious effects of reactive oxygen species (ROS) and other free radicals induced by cisplatin. This study was performed to investigate the possible protective role of antioxidant treatment with grape seed extract (GSE) on cisplatin-induced testes oxidant injury using biochemical approaches. The degree of protection produced by GSE was evaluated by determining the level of malondialdehyde (MDA) and glutathione (GSH), the activity of catalase (CAT), glutathione peroxidase (GSH-Px), were estimated from testes of rabbits. Male New Zealand white rabbits were divided into 3 groups (n=6): 1-Control group(1 ml saline. i.p) 2-Cisplatin group (a single dose of cisplatin (5 mg/kg, i.p) 3-A single dose of cisplatin (5 mg/kg, i.p) + GSE (250 mg/kg/day, i.p) for 6 days. MDA level were significantly higher when compared to Control (p < 0.05). In Cisplatin+grapes groups MDA levels were found significantly lower than cisplatin group (p < 0.05). GSH levels were decreased with cisplatin compared to control but in case of cisplatin+ GSE it increased (p < 0.05). In treated rabbits, the activity of CAT and GSH-Px was decreased in cisplatin and cisplatin+ GSE groups compared to control (p < 0.001). These results indicate that the antioxidant GSE might have a protective effect against cisplatin-induced testiculer damage and oxidative stress in rabbit.
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