Sürdürülebilir Kalkınma Amaçları
Yayınlar
- MakaleYalnız künye2018Scopus SJRQ2Phenotypic differentiation analysis: A case study in hybridizing Çoruh trout (Salmo coruhensis), Rize trout (Salmo rizeensis) and brown trout (Salmo trutta fario)
Kocabaş, Mehmet; Kutluyer, Filiz; Başçinar, Nadir
The aim of this study was to determine and compare the phenotypic variation in Çoruh trout (Salmo coruhensis), Rize trout (Salmo rizeensis), brown trout (Salmo trutta fario) and their native hybrids (S. rizeensis × S. t. fario; S. t. fario × S. coruhensis; S. rizeensis × S. coruhensis). The numbers and diameters of red and black spots were determined on operculum, fins and above and below the lateral line. The results from this study indicate that there were differences in spotting pattern, colour pattern or fin pigmentation traits between S. rizeensis, S. t. fario, S. coruhensis and their hybrids. Consequently, native S. rizeensis, S. t. fario and S. coruhensis were affected by interaction and phenotypic plastic aspects of traits in S. rizeensis were dominant in their hybrids (S. rizeensis × S. t. fario; S. rizeensis × S. coruhensis).
AtıfScopus2WoS1OpenAlex3
İncele - MakaleAçık Erişim2018Scopus SJRQ3Cypermethrin-induced in vitro alterations on oxidative stress and quality of salmo coruhensis spermatozoa
Kutluyer, Filiz; Kocabaş, Mehmet; Erişir, Mine; Benzer, Fulya
The use of insecticides has been increasing along with increasing agriculture activities and has caused deleterious environmental impacts. Non-target organisms in particular, including fish, are affected by pesticides. In this work, the impacts of cypermethrin (CYP) on sperm oxidative stress markers and sperm motility were investigated in vitro. The CYP concentrations were 0 μg L-1 control, ethanol), 1.025 μg L-1, 2.05 μg L-1 and 4.1 μg L-1. Lipid peroxidation [Malondialdehyde (MDA)], non-enzymatic antioxidants [glutathione (GSH)] and enzymatic [superoxide dismutase (SOD), glutathione peroxidase (GSH-Px) and catalase (CAT)] activities in sperm cells were examined for determination of oxidative stress status. Our findings showed that motility and survival of sperm cells significantly decreased with exposure to CYP. Biochemical assays revealed that CAT activity and levels of MDA and, GSH increased in spermatozoa based on CYP concentration while activity of GSH-Px and SOD decreased. Consequently, spermatozoa were highly sensitive to CYP exposure. It can be deduced that CYP has the potential to disrupt sperm quality and to cause to oxidative stress in sperm cells of S. coruhensis.
AtıfScopus3WoS3OpenAlex3İncele - MakaleAçık Erişim2018Scopus SJRQ1Malathion-induced spermatozoal oxidative damage and alterations in sperm quality of endangered trout Salmo coruhensis
Kocabaş, Mehmet; Kutluyer, Filiz; Benzer, Fulya; Erişir, Mine
The use of pesticides has been increased along with increasing the farming activities and has caused environmental impacts deleteriously. In particular, non-target organisms including fish can be affected by toxic effects of pesticides. Therefore, the impacts of malathion (MTN) on oxidative stress and sperm quality were investigated in vitro. The MTN concentrations used on this study were 0 (control), 75, 100, and 125 μg/L. Lipid peroxidation (MDA), non-enzymatic (GSH), and enzymatic (SOD, GSH-Px, and CAT) activities in spermatozoa were examined for determination of oxidative stress status. Our findings showed that motility rate and period of sperm cells significantly decreased with exposure to MTN. Biochemical assays revealed that CAT activity and levels of MDA, GSH increased in spermatozoa based on concentration while activity of GSH-Px and SOD decreased. Consequently, spermatozoa were highly sensitive to MTN exposure. MTN has disruptive effects on sperm quality and caused to oxidative stress in spermatozoa.
AtıfScopus14WoS14OpenAlex13
İncele - MakaleAçık Erişim2018Scopus SJRQ4Comparison of oxidant and antioxidant status of Çoruh trout (Salmo coruhensis), anatolian trout (Salmo rizeensis) and rainbow trout (Oncorynchus mykiss) spermatozoa
Kutluyer, Filiz; Kocabaş, Mehmet; Eri̇Şi̇r, Mine; Benzer, Fulya
The aim of present study was to compare oxidant and antioxidant status of Çoruh trout (Salmo coruhensis), Anatolian trout (Salmo rizeensis) and rainbow trout (Oncorynchus mykiss) spermatozoa. Fish were obtained from Uzungöl. Enzymatic antioxidant activities (superoxide dismutase, catalase, glutathione peroxidase), glutathione and lipid peroxidation (malondialdehyde) were determined in spermatozoa of three trout species. Results indicated that catalase (23.36±0.36 K/g.protein), glutathione peroxidase (74.00±1.5 U/g.protein), glutathione (0.57±1.24 µmol/g.cell) and malondialdehyde levels (6.55±2.01 nmol/g cell) were highest levels in Anatolian trout (S. rizeensis) spermatozoa. In conclusion, differences among species caused alterations in the antioxidant and malondialdehyde levels.
AtıfScopus1OpenAlex2TR Dizin1
İncele - MakaleYalnız künye2017Scopus SJRQ2Effect of Cobalt on Sperm Motility in an Endangered Trout Species, Salmo coruhensis
Kocabaş, Mehmet; Kutluyer, Filiz
Experiments were designed to examine the in vitro effect of cobalt on sperm motility of the endangered trout species (Salmo coruhensis). Sperm samples were diluted in an immobilizing solution, and activated in a motility-activation solution that was supplemented with cobalt at concentrations of 1, 10, 100 and 1000 mg/L. The percentage of motile sperm and duration of motility were determined. Cobalt concentrations of 1–100 mg/L had a positive effect on the percentage of motile sperm and duration of motility compared to the control group, while a concentration of 1000 mg/L resulted in decreases in these parameters (p < 0.05). The percentages of motile sperm at cobalt concentrations of 0, 1, 10, 100 and 1000 mg/L were 83.33% ± 0.25%, 88.33% ± 0.34%, 89.00% ± 0.57%, 90.00% ± 0.87% and 42.50% ± 0.45%, respectively; and the time durations over which the sperm remained motile were 72.00 ± 0.63, 74.83 ± 0.28, 77.40 ± 0.47, 81.14 ± 0.78, and 50.25 ± 0.67 s, respectively. This study has shown that sperm motility and duration were significantly enhanced (p < 0.05) at cobalt concentrations of 1, 10 and 100 mg/L, relative to controls, and significantly decreased at 1000 mg/L.
AtıfScopus11WoS9OpenAlex14İncele