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- MakaleAçık Erişim2026Scopus SJRQ1Ellagic Acid Mitigates Lead (Pb)-Induced Toxicity in Cyprinus carpio: A Multi-Biomarker Assessment of Hematological, Immunological, and Oxidative Stress Responses Supported by Molecular Docking
Eroglu, Mucahit; Cakmak, Mehmet Nuri; Pala, Aysegul; Uslu, Harun; Yonar, Serpil Mise; Ispir, Unal; Orhan, Cemal; Yonar, Muhammet Enis
Lead (Pb) is a widespread environmental pollutant that induces systemic toxicity in aquatic organisms primarily through oxidative stress, hematological disruption, and immune dysfunction. This study investigated the protective effects of ellagic acid (EA) against Pb-induced toxicity in common carp (Cyprinus carpio) using a multi-biomarker approach and molecular docking. Fish were assigned to six experimental groups: control, EA-treated, Pb-I, Pb-I + EA, Pb-II, and Pb-II + EA. Fish in the Pb-I and Pb-II groups were exposed to 2.5 and 5 mg/L Pb, respectively, while EA was administered via diet at 100 mg/kg for 14 days. At the end of the exposure period, hematological indices, innate immune parameters, and oxidative stress biomarkers were assessed in blood, liver, kidney, and gill tissues. Pb exposure caused marked hematological impairment, suppressed immune responses, increased malondialdehyde levels, and disrupted antioxidant defense by reducing SOD, CAT, GSH-Px, and GSH levels while increasing GST activity. In contrast, dietary EA supplementation significantly mitigated Pb-induced alterations, improved hematological and immunological responses, reduced lipid peroxidation, restored antioxidant capacity, and normalized GST activity to control levels. Molecular docking analyses further showed that EA interacts with hemoglobin and immunoglobulin M, supporting its potential role in preserving oxygen transport and immune functions under Pb-induced stress. Overall, the findings demonstrate that Pb-induced toxicity involves coordinated disruption of redox homeostasis and immune function, whereas EA exerts a multi-target protective effect through biochemical and molecular mechanisms. This study provides mechanistic insight into chemical-biological interactions and supports the potential application of natural bioactive compounds in mitigating heavy metal-induced toxicity.
- MakaleYalnız künye2025Scopus SJRQ4Bioaccumulation of copper by Gammarus pulex (L., 1758): monitoring through a statistical modelling approach
Pala, Aysegul; Ince, Muharrem; Serdar, Osman
The aim of this study is to test freshwater gammarids as ecotoxicological biomarkers. Gammarids were collected from fresh water and adapted to the laboratory environment. Then, the influence of various variables was investigated. For monitoring and detecting the bioaccumulation level in Gammarus pulex exposed to controlled copper ions and to evaluate potential human and ecological risks, the use of G. pulex as sentinel indicators of water quality is one rational approach. Copper bioaccumulation rate and fate are currently not sufficiently studied. For optimization, the experimental conditions to ensure bioaccumulation level and to find the effects of various variables on proses, a Box–Behnken experimental design was performed. Used mentioned model with statistical underpinnings for Cu2+ ions bioaccumulation have been analyzed, tested and verified and the viability of G. pulex as a biomonitor. The variables’ optimum levels (temperature; 18.1, pH; 4.3, initial concentration of Cu2+ ions, mg L−1; 11.2 and exposure time, h; 42.2) were achieved by employing the quadratic regression model. Critical role of G. pulex in freshwater biomonitoring and their potential to be evaluated as biomarkers for Cu2+ ions was confirmed. It was also explored to see if G. pulex may serve as a potential tool for estimating aquatic quality.
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İncele - MakaleYalnız künye2019Scopus SJRQ2Modelling cadmium bioaccumulation in Gammarus pulex by using experimental design approach
Serdar, Osman; Pala, Aysegul; Ince, Muharrem; Onal, Ali
Recently, although dynamic approaches have been used increasingly to describe metal bioaccumulation in aquatic organisms, the validation of such laboratory-derived modelling is rarely assessed under environmental conditions. The present study aims to evaluate and verify statistical and mathematical models for the bioaccumulation of cadmium (Cd) and to evaluate the suitability of Gammarus pulex as a biomonitor for Cd. To optimise Cd2+ ion bioaccumulation conditions a Box–Behnken design was used and whether G. pulex can be used as an estimated tool to assess aquatic quality was investigated. A Box–Behnken experimental design combined with response surface modelling (RSM) was employed for Cd2+ bioaccumulation in G. pulex. Independent variables including temperature, contact time and Cd2+ ion concentration significance and their interactions were tested using analysis of variance. The selected variables’ optimum values were obtained by solving the quadratic regression model along with by analysing the response surface contour plots. Based on Box–Behnken design, the applied model is significant because model F-value and R2 were obtained as 5121.49, 0.9998, respectively. Bioaccumulation of Cd amount was analysed using electrothermal atomic absorption spectrophotometer. The obtained data suggest that G. pulex can be evaluated as Cd biomonitor.
AtıfScopus3WoS3OpenAlex6İncele - MakaleAçık Erişim2019Scopus SJRQ4Modeling approach with Box-Behnken design for optimization of Pb bioaccumulation parameters in Gammarus pulex (L., 1758)
Pala, Aysegul; Serdar, Osman; Ince, Muharrem; Onal, Ali
The aim of this research was to evaluate the suitability of Gammarus pulex (L., 1758), an amphipod crustacean, as a bio-monitor for lead (Pb). A Box– Behnken experimental approach combined with response surface modeling (RSM) and quadratic programming was employed to increase the knowledge about Pb2+ bioaccumulation in Gammarus pulex (L., 1758). Independent variables, such as temperature ranging from 18 to 22 ºC, contact time from 8 to 48 hours, and Pb2+ ion solution concentrations from 50 to 100 µg L-1, were studied and tested using analysis of variance (ANOVA). The standardized effects of the independent variables and their interactions on the dependent variable were also investigated according to a Pareto chart. The selected variables' optimum values were obtained by solving the quadratic regression model along with analyzing the response surface contour plots. Optimum experimental conditions were obtained at 22.0 ºC, contact time of 8 hours, and Pb concentration at 69.5 µg L-1. Based on the Box-Behnken design, the applied model is significant because the model F-value and R2 were obtained at 77.80 and 0.9901, respectively. Bioaccumulation of Pb was determined using electrothermal atomic absorption spectrometry (ETAAS). The obtained data suggest that G. pulex can be used as a Pb biomonitor.
AtıfScopus4WoS6OpenAlex6İncele - MakaleYalnız künye2018Scopus SJRQ4Effect of Water Quality on Monthly Density Variation of the Endangered Southern Medicinal Leech Hirudo verbana Carena, 1820 (Hirudinea: Arhynchobdellida: Hirudinidae)
Saglam, Naim; Özbay, Ozkan; Demir, Timur; Balci, Mustafa; Pala, Aysegul; Kiliç, Ali
The species of the genus Hirudo are threatened but still used worldwide in human treatment. We studied the density changes of southern medicinal leech Hirudo verbana and some water quality parameters monthly during a year in the Akpinar Marsh, Eastern Anatolia, Turkey. Samples of H. verbana were collected using a modified unit square design and unit time collecting method as an indicator for population density. Some water parameters such as water temperature, dissolved oxygen, pH, some cationic elements (lithium, sodium, ammonium, potassium, magnesium, calcium) and anionic elements (fluoride, chloride, sulphate, nitrate, phosphate, bromide) were determined. These parameters were analysed to detect their impact on population density of this medicinal leech. The populations of H. verbena were most dense in May-June; the species was found in smallest numbers in November and hibernating in December-March. A significant correlation (p<0.05) between the number of medicinal leeches in the unit area and monthly water temperature, amount of ammonium and fluoride was determined using the Pearson correlation coefficient. In the Akpinar Marsh, the largest adult medicinal leeches (>5 g) in the fourth size group formed 12.50-27.50% of the population and the smallest (<1.0 g) in the first size group formed 7.50-38.24%.
AtıfWoS3İncele - MakaleAçık Erişim2015Scopus SJRQ1Trichlorfon-induced haematological and biochemical changes in Cyprinus carpio: ameliorative effect of propolis
Yonar, M. Enis; Yonar, Serpil Mise; Pala, Aysegul; Silici, Sibel; Saglam, Naim
Trichlorfon is among the most commonly used products to treat fish parasites in aquaculture. We investigated the effectiveness of propolis in alleviating the toxicity of trichlorfon on haematological and oxidant/antioxidant parameters in carp Cyprinus carpio. Fish were exposed to sublethal concentrations (11 and 22 mg l(-1)) of trichlorfon, and propolis (10 mg kg(-1) of fish weight) was simultaneously administered. At the end of 14 d administration, blood and tissue (liver, kidney, gill) samples were collected. Haematological changes (red and white blood cell count, haemoglobin concentration, haematocrit level and erythrocyte indices: mean corpuscular volume, mean corpuscular haemoglobin and mean corpuscular haemoglobin concentration) were determined in the blood samples, while antioxidant parameters (malondialdehyde and reduced glutathione levels and superoxide dismutase, catalase and glutathione peroxidase activities) were evaluated in the liver, kidney and gill samples. Trichlorfon led to negative alterations in the haematological and antioxidant parameters investigated. The administration of propolis alleviated this effect and suggests that fish treated with trichlorfon improve their physiological status when fed a propolis-supplemented diet.
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