Sürdürülebilir Kalkınma Amaçları
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- MakaleAçık Erişim2024Evaluation of the Effect of Different Extraction Temperatures on the Synthesis of Silver Nanoparticles from Ocimum basilicum (Basil) Plant
Aydogdu, Burcu; Unal, Ilkay
The eco-friendly green synthesis of silver nanoparticles (AgNPs) using Ocimum basilicum (basil) extract at varying extraction temperatures (40°C, 60°C, 80°C, 100°C) was investigated to determine the optimal conditions for nanoparticle formation. Analysis methods such UV-Vis spectrophotometry, Fourier Transform Infrared Spectroscopy (FTIR), X-ray Diffraction (XRD), and TransmissionElectron Microscopy (TEM) confirmed the crystalline, spherical nature of AgNPs and identified phytochemicals acting as capping and reducing agents. Notably, the extraction temperature was found to influence both the DPPH radical scavenging activity and the structural properties of AgNPs. According to TEM analysis results, it was observed that high extraction temperatures increased the nanoparticle formation efficiency but created a wide size distribution. The crystallite sizes, calculated using the Scherrer equation, for AgNPs synthesized at different extraction temperatures, were determined to be 12.45 nm, 18.77 nm, 17.76 nm, and 16.03 nm, respectively. The hydrodynamic sizes of the AgNPs were found to range between 158.1 and 333.7 nm. The study highlights the critical role of extraction temperature in the synthesis process, suggesting 40°C as the optimal temperature for achieving efficient and environmentally friendly synthesis of AgNPs with enhanced biological activities.
AtıfOpenAlex4TR Dizin1
İncele - MakaleAçık Erişim2024Biogenic Synthesis, Characterization and Degradation of Methylene Blue Dye of Zinc Oxide Nanoparticles from Different Zinc Salts Using Cinnamomum verum Extract
Aydogdu, Burcu; Unal, Ilkay
In this study, zinc oxide nanoparticles (ZnONPs) were synthesized using Cinnamomum verum extract from different zinc salts such as zinc acetate, zinc nitrate, zinc sulfate, and zinc chloride. The synthesized ZnONPs were characterized using X-ray diffraction (XRD), dynamic light scattering (DLS), Fourier transform infrared spectroscopy (FT-IR), and transmission electron microscopy (TEM). Additionally, The photocatalytic activities of ZnO nanoparticles were tested in the presence and absence of sunlight. Cinnamomum verum extract was analyzed for DPPH radical scavenging activity and total phenolic content (TPC). The study results showed that the type of zinc salt used significantly affects the morphology, size, and crystal structure of the ZnO nanoparticles. ZnONPs synthesized from zinc acetate (ZnONPsA) showed superior photocatalytic activity in the presence and absence of sunlight.
AtıfOpenAlex1İncele - MakaleAçık Erişim2024Comparison of Characteristics and Antimicrobial Activity of Synthesized Zinc Oxide And Magnetite Iron Oxide Nanoparticles Using Four Different Plant Extracts
Aytar, Mehmet; Aydogdu, Burcu; Unal, Ilkay
The aim of this study was to synthesize Zinc oxide (ZnO) and magnetite ıron oxide (Fe3O4) nanoparticles utilizing a precipitation method, employing plant extracts from Ocimum basilica(1), Cinnamomum zeylanicum(2), Lactarius salmonicolor(3) and Paeonia kesrouanensis(4) as reduction and stabilizing agents. Additionally, the antimicrobial activity of these nanoparticles against both gram-positive (S. aureus, ATCC 25923) and gram-negative (E. coli, ATCC 25922; P. aeroginosa, PAO1), bacteria as well as fungus (C. albicans 90028) was evaluated. The nanoparticles (NPs) were characterised by various analyses, including TEM, SEM, XRD, FTIR, DSL, and zeta potential. Based on the TEM image, the ZnONPs exhibited a cluster of flower-like structures, whereas the Fe3O4NPs displayed a spherical shape with a varying size distribution. The zeta potential values for ZnO NPs ranged from -5.35 to -16.9, while for Fe3O4NPs ranged from -7.43 to -20.7. All ZnO nanoparticles exhibited antimicrobial activity exclusively against the S. aureus strain, whereas Fe3O4NPs did not demonstrate any antibacterial effect.
AtıfOpenAlex4TR Dizin1İncele - MakaleYalnız künye2024Scopus SJRQ3Biosynthesis of silver nanoparticles using the aqueous extract of Rheum ribes, characterization and the evaluation of its toxicity on HUVECs and Artemia salina
Unal, Ilkay; Egri, Sinan
This study describes an eco-friendly procedure to synthesize silver nanoparticles (AgNPs) using fresh and dried stem extract of Rheum ribes. AgNPs formation was confirmed using UV-vis spectrometry. The effects of parameters such as temperature, reaction time, pH, AgNO3 concentration, and plant extract/AgNO3 ratio on NP formation were evaluated. Morphology and structural features of the particles were characterized using SEM, TEM, XRD, and FTIR. The average size, polydispersity index, and zeta potential were determined using DLS. After characterization, among the two AgNP samples, selected one; AgNP sample synthesized by dried Rheum ribes stem extracts (d-AgNPs), which exhibited narrow size distribution and high surface plasmon resonance in the range of 435-445 nm for toxicity assessment. The cytotoxic effect of d-AgNPs on HUVECs cells was evaluated with MTT analysis. Accumulation measurements were made in three periods as 24, 48, and 72 h and elimination values were examined at +24 h using ICP-MS analysis
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İncele - MakaleYalnız künye2024Scopus SJRQ2Plant extract-based biosynthesis of silver nanoparticle and silver–zinc nanocomposites-doped hydroxyapatite and its antimicrobial activity
Unal, Ilkay; Aydoğdu, Burcu; Aytar, Mehmet
Hydroxyapatite (HAp) is an essential material in the biomedical field because of its chemical composition, similar to the apatites found in bones. The lack of bactericidal properties of HAp-based materials cannot prevent the adhesion and growth of bacteria. However, it can develop composite biomaterials using nanoparticles (NPs) such as silver (Ag) and zinc (Zn), which exhibit biocidal behaviours to control resistant microorganisms. This study aimed to prepareHAp–AgNPs and HAp–Ag@ZnNPs nanocomposite and evaluate their antimicrobial properties against Gram-positive and Gram-negative bacteria. HAp powders were produced from sheep bone. An aqueous extract of Marrubium astracanicum was used as a reducing and stabilising agent for the synthesis of NPs. The composites were characterized by XRD, SEM–EDX, SEM and FTIR. In vitro biocompatibility assessment was determined by agar well diffusion tests. The HAp–Ag@ZnNPs nanocomposites showed stronger antimicrobial activity than HAp–AgNPs against bacteria and fungi, while pure HAp did not show any effect.
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İncele - MakaleYalnız künye2024Scopus SJRQ3Functionalized and Ag-Loaded Nano-Delivery System for Doxorubicin: Loading, Controlled Release and Kinetic Studies
Aydogdu, Burcu; Kaplan Ince, Olcay; Ince, Muharrem; Unal, Ilkay
Multi-walled carbon nanotubes (fMWCNTs) that functionalized and loaded Ag nanoparticles (AgNPs) were investigated to adsorp doxorubicin hydrochloride (DXR). DXR release behavior efficiency was determined using kinetic studies. DXR agent was treated into fMWCNTs@AgNPs material with different drug loading rates. The material was successfully established and characterized using transmission electron microscopy, and fourier transform–infrared spectroscopy. Thermogravimetric analysis was performed to elucidate the effect of temperature on mass loss and X-ray diffraction analysis was systematically assessed the crystalline state of DXR. Various kinetic models including Higuchi, Baker Hixson-Crowell, and power-law were applied. It was revealed that DXR has more binding affinity to the fMWCNTs@AgNPs material system surface as a nanocarrier at pH 7.4 and showed more release at pH 5.0. Functionalized material confirmed its efficiency as pH-based drug delivery system. Used material can be utilized as promising nanocarriers, and findings will contribute to the rational design of novel delivery tools for drugs.
- MakaleAçık Erişim2024Scopus SJRQ1Assessment of toxicity and oxidative stress induced by rare earth oxide nanoparticles in brine shrimp (Artemia salina)
Unal, Ilkay; Erturk Gurkan, Selin; Aydogdu, Burcu
This study meticulously explored the oxidative stress effects induced by lanthanum (III) oxide (La2O3), erbium (III) oxide (Er2O3), and yttrium (III) oxide (Y2O3) nanoparticles on Artemia salina, with the objective of evaluating the environmental toxicity of rare earth oxide nanoparticles. The characterization of the nanoparticles was conducted using a suite of advanced techniques, including X-ray diffraction (XRD), Fourier transform infrared spectroscopy (FTIR), transmission electron microscopy (TEM), dynamic light scattering (DLS), and zeta potential analysis. Artemia salina, a widely recognized model organism in ecotoxicological research, was exposed to these nanoparticles under meticulously controlled laboratory conditions. The investigation focused on quantifying oxidative stress markers, such as reduced glutathione (GSH), malondialdehyde (MDA), and antioxidant enzymes including catalase (CAT), superoxide dismutase (SOD), and glutathione-S-transferase (GST). The findings revealed significant alterations in these biomarkers, indicating nanoparticle-induced oxidative stress, which varied according to the type of nanoparticle and the duration of exposure.
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İncele - MakaleYalnız künye2020Scopus SJRQ2Assessment of impact of α-Fe2O3 and γ-Fe2O3 nanoparticles on phytoplankton species Selenastrum capricornutum and Nannochloropsis oculata
Ates, Mehmet; Cimen, Isil C. C.; Unal, Ilkay; Kutlu, Banu; Ertit Tastan, Burcu; Danabas, Durali; Aksu, Onder; Arslan, Zikri
In this study, the impact of alpha-iron oxide (α-Fe2O3, 20-40 nm) and gamma iron oxide (γ-Fe2O3, 20-40 nm) nanoparticles (NPs) on phytoplankton species Selenastrum capricornutum and Nannochloropsis oculata was investigated Characterizations of the NPs were systematically carried out by TEM, dynamic light scattering, zeta potential, X-ray diffraction, SEM, and Fourier transformation infrared spectroscopy. Acute toxicity was tested between 0.2 and 50 mg/L for each NP for a period of 72 hours exposure. γ-Fe2O3 NP inhibited development of N oculata at the rate of 54% in 0.2 mg/L group with a high mortality rate of up to 82%. α-Fe2O3 NPs were less toxic that induced 97% mortality on N oculata at 10 mg/L suspensions. In contrast, α-Fe2O3 NP inhibited growth of S capricornutum strongly (73%) in 0.2 mg/L group. γ-Fe2O3 NPs showed similar growth inhibition (72%) on S capricornutum in 10 mg/L suspensions. Despite the differential effects, the results indicated acute toxicity of α-Fe2O3 and γ-Fe2O3 NPs on N oculata and S capricornutum.
AtıfScopus27WoS20OpenAlex25İncele - MakaleYalnız künye2020Scopus SJRQ2Assessment of Oxidative Stress on Artemia salina and Daphnia magna After Exposure to Zn and ZnO Nanoparticles
Ates, Mehmet; Danabas, Durali; Ertit Tastan, Burcu; Unal, Ilkay; Cicek Cimen, Isil Canan; Aksu, Onder; Kutlu, Banu; Arslan, Zikri
In this study, the effect of zinc nanoparticles (Zn NPs) and zinc oxide nanoparticles (ZnO NPs) on Artemia salina and Daphnia magna, the primary consumer organisms were investigated. In this sense, investigation of trophic transfer and ecological sustainability potentials among living things, such as fish and crustaceans that are at the top of the food chain were also aimed. Zn NPs in the size of 40–60 nm and 80–100 nm and ZnO NPs (10–30 nm) were administered to A. salina and D. manga (respectively in total 105000 and 14000 individuals) in seven groups (Control, 0.2, 1, 5, 10, 25 ve 50 ppm) with three repetitions for a period of 72 h. Intensive and possible misuse of nanoscale materials is one of the biggest threats to the environment and all living things worldwide. Therefore, the control mechanisms for the use of NPs need to be established.
AtıfScopus29WoS19OpenAlex32İncele - MakaleYalnız künye2020Scopus SJRQ2An Evaluation Research About Effects of Characterized Cadmium Selenide (CdSe) and Lead Selenide (PbSe) Quantum Dots on Brine Shrimp (Artemia salina)
Ates, Mehmet; Tastan, Burcu Ertit; Danabas, Durali; Cicek-Cimen, Isil Canan; Aksu, Onder; Kutlu, Banu; Unal, Ilkay; Arslan, Zikri
Quantum dots (QDs), such as cadmium selenide (CdSe) and lead selenide (PbSe) exhibit excellent optical, magnetic and chemical properties due to their extremely size (ca. 1–10 nm) and are attractive semiconductor nanomaterials for optical studies and energy storage. In this study, aqueous synthesis of CdSe and PbSe QDs in a size range of 2–10 nm was described. Synthesized QDs were characterized using SEM and TEM, DLS, zeta potential, FTIR, EDX and XRD. Highest accumulation (72.5 ± 5.8 mg L−1) of PbSe QDs occurred at 10 ppm suspensions. In general accumulation increased up to 48 h exposure then fluctuate tended to decline. For CdSe QDs, accumulation tended to decrease for 72 h exposure except that for 5 ppm groups. For the elimination period, in general, the elimination levels of PbSe and CdSe QDs from exposed individuals decreased (p < 0.05) even it has some fluctuate.
AtıfScopus6WoS4OpenAlex5İncele - MakaleYalnız künye2020Scopus SJRQ1Effects of Zn and ZnO Nanoparticles on Artemia salina and Daphnia magna Organisms: Toxicity, Accumulation and Elimination
Danabas, Durali; Ates, Mehmet; Ertit Tastan, Burcu; Cicek Cimen, Isil Canan; Unal, Ilkay; Aksu, Onder; Kutlu, Banu
In the study, Zn in the size of 40–60 nm and 80–100 nm and ZnO in the size of 10–30 nm were applied to A. salina and D. magna individuals in 7 groups with 3 repetitions. Measurements were made at 24th, 48th and 72nd hours and elimination values were examined at +24 h. LC50 values of NPs were determined and chemical analysis (metal accumulation and elimination), ion quantities which were given to the environment and the survival rates of organisms were determined after the exposure. According to the results of phase contrast microscopy, it was found that both experimental organisms absorbed the NPs in the medium level. In the toxicity results of D. magna, it can be said that Zn NP (40–60 nm) has a highly toxic effect only at 50 ppm concentration for 48 h and lethal dose can be accepted as of 5 ppm at the end of 72 h. In A. salina individuals, it is clearly seen that there is an increase in mortality in organisms parallel to the dose increase. Although all NPs were applied to organisms in low doses corresponding to environmental values, it was observed that toxic effect was in parallel with the increase in time. It is clearly known that there is the inverse proportion between the size of NPs and the toxic effect. The smaller the size of NPs is, the higher the toxic effect becomes When the results of Zn accumulation and elimination of A. salina and D. magna individuals exposed to the Zn and ZnO NPs were examined; it was found that accumulation and elimination occurred in parallel with the increase in concentration at each application hour and elimination. Intensive and possible misuse of nanoscale materials is one of the biggest threats to the environment and all living things worldwide.
AtıfScopus63WoS59OpenAlex71İncele - MakaleYalnız künye2017Scopus SJRQ3Characterization of antioxidant activity, vitamins, and elemental composition of ciris (Asphodelus aestivus L.) from Tunceli, Turkey
Unal, Ilkay; Kaplan Ince, Olcay
Ciris (Asphodelus aestivus L.), which has been used for a wide range of medicinal purposes and is indigenous in Tunceli, Turkey, was collected from the eastern and western parts of the city. The goal of this study was to determine the vitamins, essential elements, and antioxidant activity of this natural product. The 2,2-diphenyl-1-picrylhydrazyl-hydrate radical scavenging activity, metal chelating activity, reducing power, total phenolics, and Vitamin A, Vitamin C, sodium, potassium, phosphorus, magnesium, calcium, copper, manganese, and zinc concentrations were determined in ciris extracts. The concentrations of Vitamin A, Vitamin C, and Mn of ciris from west Tunceli were higher than samples from the east.
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