Sürdürülebilir Kalkınma Amaçları
Yayınlar
- MakaleYalnız künye2025Scopus SJRQ2Phylogenetic analysis of the nrDNA ITS and cpDNA rbcL sequences of some endemic Dianthus (Caryophyllaceae) species from Türkiye
Sevindik, Emre; Güner, Özal; Yolcu, Seher; Başköse, İsa; Paksoy, Mehmet Yavuz; Khadivi, Ali; Tunç, Yazgan; Yaman, Mehmet
In this study, the phylogenetic relationship of four species of Dianthus genus which are endemic and distributed in Türkiye, including D. zederbaueri Vierch, D. varankii Hamzaoğlu & Koç, D. halisdemirii Hamzaoğlu & Koç, and D. goekayi Kaynak, was determined using nuclear DNA (nrDNA) internal transcribed spacer (ITS) and chloroplast DNA (cpDNA) rbcL regions. After sampling from the plant, DNA was extracted and the ITS (ITS4-ITS5A) and rbcL (rbcLaF-rbcLaR) primers were used to perform PCR amplification. Sequencer 5.4.6. was used to edit the sequences. MEGA 11 software was used to determine the nucleotide ratios, genetic distance matrix, and π = nucleotide diversity. Some species of Dianthus available in the National Center for Biotechnology Information (NCBI) were used to construct maximum likelihood (ML) phylogenetic trees. As a result, based on the ITS analysis π = nucleotide diversity was determined as: 0.001543, and the result of the rbcL analysis π = nucleotide diversity was determined as: 0.000000. In the ITS results, D. halisdemirii, D. superbus, and D. varankii appeared together, while D. zederbaueri and D. aculeatus appeared together. The rbcL results showed that D. zederbaueri and D. varankii were closely related, but the relationship between D. halisdemirii and D. goekayi could not be determined. Furthermore, in the phylogenetic tree constructed by taking the ITS and rbcL sequences of Dianthus, Gypsophila, Silene, Arenaria, Saponaria, Cerastium, Stellaria, Petrorhagia, Minuartia, Agrostemma, and Polycarpon species from NCBI using ITS and rbcL sequences, our endemic Dianthus species appeared in the same group with Petrorhagia, Gypsophila, and Saponaria species. To conclude, our ITS and rbcL results were consistent in determining the phylogenetic relationships of our Dianthus species with other species.
AtıfScopus1
İncele - MakaleAçık Erişim2022Scopus SJRQ1Evaluation of Iron and Zinc Contents of Some Fish Species
Karaaslan Ayhan, Nagihan; Yaman, Mehmet
In this study, Fe and Zn contents in the muscle of different fish species (Engraulis encrasicolus, Trachurus trachurus, Mullus barbatus, Mugil cephalus, Pomatomus saltatrix, Dicentrarchus labrax, Scomber scombrus, Sparus aurata) were determined using flame atomic absorption spectrometry (FAAS). The highest Fe and Zn contents among studied fish species were found in Engraulis encrasicolus (7.13 ± 0.19 mg/kg) and Sparus aurata (11.00 ± 0.28 mg/kg). The lowest Fe and Zn contents were determined in Dicentrarchus labrax (3.12 ± 0.09 mg/kg) and Pomatomus saltatrix (5.50 ± 0.05 mg/kg). Moreover, the results were evaluated considering the estimated daily intakes (EDI) of these elements for adults. The estimated daily intakes were varied from 0.0101 to 0.0231 mg/kg bw/day for Fe and 0.0178 to 0.0357 mg/kg bw/day for Zn in fish species. In addition, the hazard quotient (HQ) and the hazard index (HI) values were calculated for risk assessment, and it was determined that these values were below 1 for fish species.
AtıfScopus10WoS8OpenAlex9
İncele - MakaleAçık Erişim2021Scopus SJRQ3Determination of fatty acids in Allium tuncelianum (Tunceli garlic) by gas chromatography with flame ionization detection (GC–FID)
Ugur, Yilmaz; Karaaslan-Ayhan, Nagihan; Icen, M. Sina; Bicim, Tulin; Erdogan, Selim; Yaman, Mehmet
Garlic has an important place in daily nutrition. It is consumed by people because of its antimicrobial, antibacterial, and antioxidant properties, and it is also known to be good for many diseases. Along with these properties of garlic, its fatty acid content is also important. The goal of this study is to determine the fatty acids in Allium tuncelianum (Tunceli garlic), which grows naturally in Tunceli, Turkey, by gas chromatography with flame ionization detection (GC–FID). Fifteen of 37 fatty acids studied were detected in Allium tuncelianum, and the primary fatty acids in the sample extracts were determined to be the polyunsaturated fatty acid (PUFA) linoleic acid (C18:2n6c, 41.595%) and the monounsaturated fatty acid (MUFA) oleic acid (C18:1n9c, 33.302%). Small quantities of saturated fatty acids (SAFAs) were also present, including palmitic acid (C16:0, 11.739%), henoicosanoic acid (C21:0, 6.946%), and stearic acid (C18:0, 2.362%).
AtıfScopus12WoS8OpenAlex11İncele - MakaleAçık Erişim2021Scopus SJRQ3Determination of anthocyanins and anthocyanidins in the wild grape (Vitis sylvestris Gmelin) by high-performance liquid chromatography-diode array detection (HPLC-DAD)
Karaaslan-Ayhan, Nagihan; Yaman, Mehmet
The determination of anthocyanins and anthocyanidins in wild grapes (Vitis sylvestris Gmelin) from Tunceli-Turkey are reported together with the influence of the extraction conditions on the results. Representative sets of anthocyanins (delphinidin-3-o-glucoside, cyanidin-3-o-glucoside, pelargonidin-3-o-glucoside, malvidin-3-o-glucoside) and anthocyanidins (delphinidin, cyanidin, pelargonidin, malvidin) were simultaneously determined by high performance liquid chromatography with diode array detection (HPLC-DAD). The extraction conditions including the solvent type, extraction time and liquid:solid ratio (v:w) were optimized. The anthocyanin and anthocyanidin contents changed significantly depending on the extraction conditions. Malvidin-3-o-glucoside was identified to be the primary compound.
AtıfScopus10WoS8OpenAlex11İncele - MakaleYalnız künye2018Scopus SJRQ3SEASONALLY VARIATIONS OF ARSENIC SPECIES IN CHICKEN MEATS CONSUMED BY TURKISH PEOPLE WITH HG-AAS
Kaya, Gokce; Karaaslan, Nagihan M.; Yaman, Mehmet
The aim in this study is speciation and determination of arsenic in chicken meat matrix. For this purpose, chicken meat samples consumed commonly by Turkish people belonging to five different companies in Turkey were obtained from markets once per 15 days during 14 months. The samples were digested in microwave oven by using both nitric and hydrochloric acid. It was found that microwave digestion method can be used successfully in the speciation analysis of arsenic. The determination and speciation of As in solutions were carried out by hydride generation-atomic absorption spectrophotometry (HG-AAS). From the observed results, concentrations of As (V) were ranged between 3.0-14.0 ng g(-1) (mean 7.3 ng g(-1)) for breast tissues and 3.0-9.0 ng g(-1) (mean 5.1 ng g(-1)) for leg tissues. The concentrations of As (III) in all studied chicken tissues were found lower than the limit of detection (2 ng g(-1)).
AtıfWoS2İncele - MakaleAçık Erişim2018Scopus SJRQ2Anthocyanin profile of strawberry fruit as affected by extraction conditions
Karaaslan, Nagihan M.; Yaman, Mehmet
The purpose of the study was to investigate the effect of extraction conditions to determine anthocyanin compounds in strawberry fruit. Anthocyanin profile of this fruit were determined via high-performance liquid chromatography-electrospray ionization-mass spectrometry. Flow rate of mobile phase, fragmentor potential, injection volume, and column temperature were applied to be optimum values as 0.5 mL min−1, 110 V, 5 µL, and 30°C, respectively. The extraction process was the important step in the isolation and identification of anthocyanins. For this purpose, anthocyanin contents in strawberry fruit were examined in terms of solvent type, extraction time, and liquid:solid ratio (v:w). The best extraction conditions in this fruit were found to be methanol as solvent, 30 min as extraction time and 1:1 as liquid:solid ratio (v:w). The results showed that the anthocyanin contents of strawberry fruit change by altering extraction conditions.
AtıfScopus50WoS39OpenAlex48İncele - MakaleYalnız künye2018Scopus SJRQ3Assessment and ICP-MS determination of toxic metal content (Cd, Cr, Ni, and Pb) in Ibrkish chicken meat for use as bioindicator for human health
Karaaslan, Nagihan M.; Yaman, Mehmet
The aim of this study was to determine the toxic metal concentrations in chicken meat consumed by the Turkish population and to establish health risk assessment. The concentrations of cadmium (Cd), chromium (Cr), nickel (Ni), and lead (Pb) in chicken meat produced by seven different suppliers were determined using inductively coupled plasma mass spectrometry (ICP- MS). The Cd concentrations in all samples were found to be lower than the maximum allowable concentration (MAC) of 50 Hg kg-1. The Cr and Ni concentrations in all samples were lower than 700 and 450 μg kg-1, respectively, with the exception of Cr in the samples from one producer and of Ni from two other producers. The Pb concentrations (max. 413 μg kg-1) in the samples from three producers were found to be higher than the MAC of 100 μg kg-1. Although the concentrations of lead in some samples exceed the limits set by the authorities, risk of developing cancer over a human lifetime was found to be not significant (between 3 to 16 per 1,000,000 persons).
AtıfScopus8İncele - MakaleAçık Erişim2017Scopus SJRQ2Optimization of an Analytical Method for Determination of Pyrene in Smoked Meat Products
Ince, Muharrem; Kaplan Ince, Olcay; Yaman, Mehmet
In the present study, an analytical method was optimized for determination of pyrene by solid-phase extraction in Turkish foodstuffs including chicken and grilled meat samples. In accordance with this purpose, all of the parameters that may affect the signal of pyrene during analysis processes were optimized using high-performance liquid chromatography–mass spectrometry system. The optimized parameters for column temperature, injection volume, mobile-phase flow rate, and fragmentor potential were found to be 45 °C, 60 μL, 0.6 mL min−1, and 130 V, respectively. The optimized high-performance liquid chromatography–mass spectrometry method was applied for the determination and evaluation of pyrene in food samples that include chicken and grilled meat. The pyrene amount in meat samples was obtained in the range from 2.3 to 4.4 μg kg−1 for the normal grilled meat and chicken samples and from 12 to 135 μg kg−1 for exceedingly grilled samples. The results show that the concentration of pyrene is significantly dependent on the type of meat sample and cooking source. The limit of detection and quantitation was found to be 0.5 μg L−1 and 1.53 μg L−1, respectively.
AtıfScopus5WoS5OpenAlex7İncele - MakaleÖn baskısı açık2016Scopus SJRQ1Determination of anthocyanins in cherry and cranberry by high-performance liquid chromatography-electrospray ionization-mass spectrometry
Karaaslan, Nagihan M.; Yaman, Mehmet
Anthocyanins are a group of widespread natural phenolic compounds in vegetables and fruits. The anthocyanins have a wide range of applications due to the antioxidant, anticancer and anti-inflammatory properties. In this study, anthocyanins (delphinidin-3-o-glucoside, cyanidin-3-o-glucoside, pelargonidin-3-o-glucoside and malvidin-3-o-glucoside) in cherry and cranberry were determined using high-performance liquid chromatography-electrospray ionization-mass spectrometry (HPLC-ESI-MS). The anthocyanins were separated using gradient elution and a reserved-phase analytical column before identification by high-performance liquid chromatography-electrospray ionization-mass spectrometry. A high-performance liquid chromatography-electrospray ionization-mass spectrometry method was optimized for the determination of anthocyanins in cherry and cranberry. Furthermore, in this study, we investigated extraction conditions of fruit samples as well as determination of optimum HPLC-ESI-MS conditions. This study is novel in terms of simultaneously examining both optimization of HPLC parameters and extraction conditions. Obtained optimum conditions were used for the determination as the quantitative and qualitative analysis of anthocyanins in cherry and cranberry. The content of anthocyanins on the basis of wet weight in cherry and cranberry samples was determined for delphinidin-3-o-glucoside < d.l. (detection limit) and < d.l., for cyanidin-3-o-glucoside varied from 3.5 +/- A 0.4 to 8.3 +/- A 1.1 mg kg(-1) (average 5.8 +/- A 0.8 mg kg(-1)) and 9.8 +/- A 1.4 to 18 +/- A 3.0 mg kg(-1) (average 13.2 +/- A 1.8 mg kg(-1)), for pelargonidin-3-o-glucoside < d.l. and varied from 136 +/- A 19 to 233 +/- A 35 mg kg(-1) (average 185.3 +/- A 28 mg kg(-1)), for malvidin-3-o-glucoside < d.l. and < d.l., respectively.
AtıfWoS25OpenAlex29İncele - Proje2013Küresel optimizasyon için yapay kimyasal reaksiyon algoritması geliştirme
Özer, A. Bedri; Yaman, Mehmet; Yasyerli, Sena
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- MakaleYalnız künye2013Scopus SJRQ3FLAME ATOMIC ABSORPTION SPECTROMETRIC DETERMINATION OF NICKEL AND CHROMIUM IN VARIOUS PLANT LEAVES USED AS BIOMONITORS IN ENVIRONMENTAL POLLUTION
Avci, Halim; Kaya, Gokce; Akdeniz, Ismail; Ince, Muharrem; Yaman, Mehmet
Levels of nickel and chromium in leaves of 14 plant species grown in industrial sites of Gaziantep and surrounding were examined for biomonitoring of atmospheric pollution using flame atomic absorption spectrophotometry (FAAS). The plant leaf samples were collected from different locations with different degrees of metal pollution. The metal levels in the plants were in the ranges of (with mean values) 0.4-28.0 (3.1) mg kg(-1) for Ni and 0.1-6.6 (1.5) mg kg(-1) for Cr. On the other hand, the Ni and Cr levels in the plant leaf samples collected from control) areas were in the ranges of 0.4-2.1 (1.1) and 0.1-1.4 (0.58) mg kg(-1), respectively. It was concluded that Pinus nigra L., Acacia robinia L., Platanus orientalis L., Nerium oleander L., Pyracantha coccinea M.Roem. and Populus nigra L. for Ni, Pinus nigra L., Acacia robinia L., Olea europaea L. and Nerium oleander L. for Cr could be biomonitors. Therefore, it was demonstrated that the plant leaves could be used for heavy metal air pollution monitoring in urban and industrial regions.
AtıfWoS10İncele - MakaleAçık Erişim2013Scopus SJRQ1Fractionation of Ni, Cr and Cu from Soil by Sequential Extraction Procedure and Determination by Inductively Coupled Plasma Optical Emission Spectrometry
Akkus, Sukran; Bal, Tulin; Karaaslan, Nagihan M.; Yaman, Emre; Kilinc, Ersin; Yaman, Mehmet
Five-step sequential extractions were employed to fractionation of Ni, Cr and Cu in soil polluted by anthropogenic activities and determine the mobility of the metals. Twelve samples were collected on an agricultural area that was located near an airport and intercity roads in Elazig-Turkey. Exchangeable, organically bounded, carbonate bounded, adsorbed species on Fe and Mn oxides and residual species (except silicates) of Ni, Cr and Cu were extracted into solution by using CaCl2, Na4P2O7, Na(2)EDTA, NH2OH-HCl and HNO3-H2O2, respectively. Mobile metal concentrations in fractions and total recoverable in soils were determined by using inductively coupled plasma-optical emission spectrometry (ICP-OES). Total recoverable Ni, Cr and Cu concentrations were in the range of 40-119, 45-126 and 23-72mgkg(-1), respectively. It was observed that total concentrations of metals in some of the samples were higher than the permitted values. The sum of the mobile percentages of metals was found to be lower than 50%. The Ni, Cr and Cu percentages for exchangeable species are in the ranges of 0.18-1.64, 0.03-0.59 and 0.42-2.53%, respectively.
AtıfWoS6OpenAlex5İncele - MakaleYalnız künye2012Scopus SJRQ4Use of Atomic Absorption Spectrometry in Assessment of Biomonitor Plants for Lead, Cadmium and Copper Pollution
Kaya, Gokce; Yaman, Mehmet
Eleven plant species were collected from the vicinity of lead-battery plant in the city of Gaziantep, Turkey. Lead, cadmium and copper concentrations in the soil and leaves of plants were determined by atomic absorption spectrometry. Lead, Cd and Cu concentrations in the soil samples taken from battery area were found to be in the ranges of 304 similar to 602, 0. 4 similar to 0. 44 and 31 similar to 37 mg . kg(-1), respectively. Significantly increased lead concentration up to 2 750 mg . kg(-1) was found in the leaves of Eleagnus angustifolia L. plant. The lead concentrations in the other plant leaves taken from 50 m around battery factory followed the order Ailanthus altissima > Morus sp. > Juglans regia L. > Ficus carica L. > Cydonia oblonga Miller > Prunus x domestica L. The plants, Populus nigra L., Eleagnus angustifolia L. and Salix sp. were found useful for Cd, and the plant, Eleagnus angustifolia L. for Pb, to be considered as potential biomonitor. Especially, leaves of trees and plants taken from the distance of 50 m from battery plant have relatively higher Pb concentrations. Therefore, people who and animals which live in this area and benefit from these soil and plants have vital risks.
AtıfWoS12İncele - MakaleYalnız künye2012Scopus SJRQ4High Performance Liquid Chromatography-Mass Spectrometry for Determination of Benzo[a]pyrene in Grilled Meat Foods
Ince, Muharrem; Yaman, Mehmet
The concentrations and varieties of benzo[a]pyrene were determined in Turkish foodstuffs including grilled meat and chicken samples by high performance liquid chromatography-mass spectrometry (HPLC-MS). The parameters that are thought to affect the response in the HPLC-MS analysis were optimized. The optimized conditions were found to be 0.75 mL min(-1) for flow rate of mobile phase, 45 mu L for injection volume, 55 degrees C for column temperature and 140 V for fragmentor potential. The optimized method was applied for the determination of benzo[a]pyrene concentrations in grilled meat and chicken foods. The obtained concentrations were found in the range of 2.7-8.3 mu g kg(-1) for the normal grilled meat and chicken samples and 72-138 mu g kg(-1) for over-grilled samples. The results showed that the concentrations of benzo[a]pyrene were significantly dependent on the kind of meat and the cooking time and source. The limits of detection and quantitation were found to be 0.7 and 2.33 mu g L-1, respectively.
AtıfWoS1İncele - MakaleAçık Erişim2012Scopus SJRQ3DETERMINATION OF TRACE METALS IN PLANT LEAVES AS BIOMONITOR OF POLLUTION EXTENT BY A SENSITIVE STAT-AAS METHOD
Kaya, Gokce; Yaman, Mehmet
The aim of this study is to assess the potential of bioindicator/biomonitor plants to determine pollution extent of toxic metals. Lead, cadmium, and copper concentrations in the plants including Platanus sp., Olea europaea L., Vitis vinifera L., and Armeniaca sp. taken from the industrial area of the city of Gaziantep, Turkey, and control location were determined by using STAT-atomic absorption spectrometry. Lead concentration in leaves of Platanus sp. taken from 50m around the battery plant was found to be higher than 3,000 mg kg(-1). The observed Pb concentration in this plant was 40 times higher than that in Armeniaca sp. grown in the same location. The limits of detection for plants were found to be 0.050 mg kg(-1) for Pb and Cu and 0.005 mg kg(-1) for Cd. It was concluded that the Platanus sp. plant can be considered as both biomonitor and hyperaccumulator.
AtıfWoS22OpenAlex17İncele - MakaleAçık Erişim2011Scopus SJRQ4Determination of Elements in Thermal Springs for Monitoring Pre-Earthquake Activites by ICP-MS
Yaman, Mehmet; Sasmaz, Ahmet; Kaya, Gokce; Ince, Muharrem; Karaaslan, Nagihan M.; Ozcan, Cemile; Akkus, Sukran
AtıfOpenAlex3İncele - MakaleAçık Erişim2011Scopus SJRQ2Sequential extraction of cadmium in different soil phases and plant parts from a former industrialized area
Kaplan, Olcay; Ince, Muharrem; Yaman, Mehmet
Cd concentrations in mobile phases of soil are more representative than total Cd concentration for estimating Cd bioavailability, physicochemical reactivity and mobility. In this study, selective sequential extraction procedures were used to determine Cd in different soil phases. Soil samples and plants grown in these soils were collected from a serpentine and copper-mining area in Maden-Elazig-Turkey. The extracted fractions were exchangeable/carbonate, reducible-iron/manganese oxides, oxidizable-organic matter and sulfides, and residual phases except silicates. Concentrations of Cd in soils and plant samples were determined by flame atomic absorption spectrometry and inductively coupled plasma-mass spectrometry. We found that Cd concentrations in the EDTA and NH(2)OH center dot HCl extracts are higher in most soil samples compared to the other extracts. We conclude that Cd levels in mobile phases are unexpectedly high. The observed Cd concentrations are in ranges of 0.03-3.4 mg kg(-1) for soil and 0.02-2.5 mg kg(-1) for plant parts. The percentages of cadmium up to 56% in exchangeable and carbonates fractions were observed to be significantly higher than in those values less than 2% reported in literature. This study has shown that the modified extraction method can be usefully applied to determine Cd concentrations in potentially mobile phase of soil. Furthermore, it was concluded that Brassicasea and Rumex leaves can be used as hyperaccumulator plants because their translocation factor and/or enrichment coefficient values were found to be higher than 1.0.
AtıfWoS29OpenAlex29İncele - MakaleAçık Erişim2011Scopus SJRQ2Sequential and Selective Extraction of Copper in Different Soil Phases and Plant Parts from Former Industrialized Area
Kaplan, Olcay; Kaya, Gokce; Yaman, Mehmet
Copper (Cu) is an essential trace element for all living organisms; however, excess amounts in soil, plants, and food have negative impacts on the environment and human health. The aim of this study was to determine Cu levels in different chemical phases of soils and the relationship between Cu levels in soil phases and concentrations in plants. Soils and plants grown in these soils from an industrialized area in Turkey were analyzed using a selective and four-stage sequential extraction procedure. Copper levels in exchangeable fractions were found up to 658 mg kg(-1) while total levels were in the range of 133-5609 mg kg(-1). Copper concentrations in plant parts (roots and stem) were in the range of 2.6-240 mg kg(-1). The exchangeable forms of Cu were in the range of 3-22% of total Cu concentrations. The relationships were observed between soil Cu and Rumex plant Cu, and soil Cu and root of Brassicasea plant Cu.
AtıfWoS11OpenAlex10İncele - MakaleYalnız künye2010Scopus SJRQ3Use of Flame Atomic Absorption Spectrometry in Diagnosis of Cancerous and Non-Cancerous Breast Tissues: Comparison of Metal Concentrations
Kaya, Gokce; Akdeniz, Ismail; Ozcan, Cemile; Kilinc, Ersin; Yaman, Mehmet
- MakaleAçık Erişim2010Scopus SJRQ2Flame Atomic Absorption Spectrometric Determination of Pb, Cd, and Cu in Pinus nigra L. and Eriobotrya japonica Leaves Used as Biomonitors in Environmental Pollution
Kaya, Gokce; Ozcan, Cemile; Yaman, Mehmet
The assessment of trace metal pollution in Gaziantep city-Turkey has been studied using plant leaves of Pinus nigra L. and Eriobotrya japonica as biomonitor. The concentrations up to 3,056 mg Pb kg(-1) in the needles of Pinus nigra L., and 367 ng Cd g(-1) in the leaves of Eriobotrya japonica were determined. The observed Cu concentrations were in range of 1.6-7.1 mg kg(-1). The Pb, Cd, and Cu levels in soils were determined to be in the range of 17-602, 0.142-0.656, and 12-38 mg kg(-1), respectively. It was concluded that Pinus nigra L. can be considered as both biomonitor of atmospheric Pb pollution and hyperaccumulator plant.
AtıfWoS41OpenAlex37İncele
