Sürdürülebilir Kalkınma Amaçları
Yazarlar
- Akin, S.(1)
- Aksu, O.(1)
- Altinterim, B.(1)
- Banu, Kutlu(1)
- Danabas, S.(1)
- Gulec, Kucukgul A.(1)
- Gulsafak, I(1)
- Kayim, M.(1)
- Kucukgul, A.(1)
- Yildirim, N. C.(1)
- Yildiz, M.(1)
Yayınlar
- MakaleYalnız künye2019Scopus SJRQ3Bio-functions of carvacrol-supplemented feeds on lipopolysaccharide-induced rainbow trout (Oncorhynchus mykiss Walbaum, 1792)
Kucukgul, A.; Gulsafak, I
To investigate the effects of carvacrol supplementation in rainbow trout induced by lipopolysaccharide (LPS), relative gene expressions levels of some pro-inflammatory cytokines and apoptosis markers in kidney and liver were measured. Fish with a mean weight of 44.71 +/- 1.33 g were studied using four different treatments with three replicates each. Inflammation and apoptosis were performed using LPS of Escherichia coli (25 mu g ml(-1)) except in the control group and only carvacrol (100 mu g ml(-1) diet) containing diets were fed to this group (CAR). The last group was the infected fish fed carvacrol supplemented diet (+CAR). Kidney and liver tissues were removed 3 days after to determine the levels of interleukin-1 beta (IL-1 beta), interferon gamma (INF-gamma), caspase 3 (Cas 3), caspase 8 (Cas 8) using Real-Time PCR analyses. IL-1 beta expressions of both kidney and liver was significantly decreased (12.9 and 2.14 fold, respectively) in LPS treated cells (p<0.05). While IFN-gamma expression was up regulated in kidney, it had down regulation in liver. LPS decreased both Cas 3 and 8 expressions in kidney but increased in liver. +CAR increased expressions of IL-1 beta and IFN-gamma compared with the control (up to 2-3 fold) in all tissues except caspase gene expressions that were similar in the control. In all tissues, IL-1 beta and IFN-gamma expressions increased in +CAR group, expect IFN-gamma in the kidney. However, Cas 3 and 8 expressions including apoptosis was induced by up-down regulations in all tissues compared to LPS-injected fish. The results showed that carvacrol had pro-inflammatory and apoptotic effects especially on liver tissue of LPS-induced inflammation model of Oncorhynchus mykiss.
AtıfWoS4OpenAlex3
İncele - MakaleYalnız künye2014Scopus SJRQ3Assessment of immunomodulator biomarkers (Tnf-α, Il-1β and Il-6) in liver of Capoeta umbla for biomonitoring of pollution in Uzuncayir Dam Lake (Tunceli, Turkey)
Yildirim, N. C.; Danabas, S.
This study was aimed to monitor water pullution of Uzuncayir Dam Lake using the changes of IL-6, IL1 beta and TNF-alpha levels in Capoeta umbla (Heckel, 1843) liver tissue at ten stations in March and September 2011. In this study C. umbla (Heckel, 1843) was used as the indicator organism. Tumor necrosis factor-alpha (TNF-alpha), interlekuin1 beta (IL-1 beta) and interleukin-6 (IL-6) levels were determined in samples of the liver tissue by ELISA kit. The lowest mean IL-1 beta levels were found at station 6. The mean IL-1 beta was reached its maximum level at station 2. The difference between the mean levels of IL-6 was found to be significant (p<0.05) amoung stations. The IL-6 levels were significantly increased in September at stations 1, 2, 7 and 8 (p<0.01) compared to the values in March. The mean levels of TNF-alpha were found to be significant (p<0.05) amoung stations. The TNF-alpha levels significantly decreased in September at stations 1 and 9 (p<0.01). TNF-alpha, IL-1 beta and IL-6 levels in C. umbla can be used as early diagnostic indicators against adverse environmental events and useful and reliable bioindicators in determining the pollution of the aquatic ecosystem.
AtıfWoS16İncele - MakaleYalnız künye2013Scopus SJRQ3Determination of lethal concentration (LC50) values of Cinnamomum zeylanicum hydrosol on carp fish
Gulec, Kucukgul A.; Altinterim, B.; Aksu, O.
In this study, lethal concentration (LC50) values of cinnamon hydrosol (Cinnamomum zeylanicum) on carp (Cyprinus carpio) were investigated. In practice, experimental setup was constituted 30 fish (a total of 180 fish with 30 control fish) to be placed in three replicates. Hydrosol was added into aquariums at the doses of 0.5, 1, 2.5, 5 and 10% and determined mortality times of carp exposed to these concentrations. Percentage death of fish calculated in these concentrations. Mortality was observed at all treatments exception of dose of 0.5 %. The results indicate that the hydrosol had swimming changes, lethargy, lack of breath and leaning to the depth of the aquariums at all of the concentrations. The results of regression analysis indicated that the mortality rate (Y) is positively correlated the concentration (X) having a regression coefficient (R) close to one in each case. While the 1 h LC50 value (with 95% confidence limits) of the safe dose of C. zeylanicum hydrosol was estimated at LC50=4.39%, 2 h LC50 value=2.629%, and 12 h LC50 value= 1.027%.
AtıfWoS10İncele - MakaleYalnız künye2013Scopus SJRQ3The Anesthetic Effects of Clove Oil and 2-Phenoxyethanol on Rainbow Trout (Oncorhynchus mykiss) at Different Concentrations and Temperatures
Yildiz, M.; Kayim, M.; Akin, S.
In this study, anesthetic effects of five different concentrations of 2-phenoxyethanol (0.2, 0.3, 0.4, 0.5 and 0.6 ml/L) and clove oil (0.50, 0.75, 1.00, 1.25 and 1.50 ml/L) on rainbow trout (Oncorhynchus mykiss) were studied at temperatures 7, 13 and 18 degrees C. For this purpose, 900 fish (39.08 +/- 1.13 g and 15.48 +/- 0.21 cm) were used in the experiment. Induction time of 2-phenoxyethanol and clove oil varied between 1.05 and 3.36 min at all concentrations, except for 0.2 ml/L (for 2-phenoxyethanol only) and at every temperature application. Full recovery time occurred between 2.44 and 7.14 min for 2-phenoxyethanol and 3.23 - 6.11 min for clove oil. It was found that full recovery times significantly increased with increase in 2-phenoxyethanol concentrations (r(2)=0.81). The same increasing trend was observed in clove oil, but the increase was not strong compared to 2-phenoxyethanol (r(2)=0.21). On the other hand, full induction times of 2-Phenoxyethanol and clove oil significantly declined with the increase in concentrations (r(2)=0.74; r(2)=0.84 for 2-phenoxyethanol and clove oil, respectively). Based on the ideal induction (less than 3 min) and recovery (less than 5 min) time criteria, it can be suggested that the most appropriate concentrations for rainbow trout were 0.3, 0.4 and 0.5 ml/L for 2-phenoxyethanol and 0.50, 0.75 and 1.00 ml/L for clove oil.
AtıfWoS16İncele - MakaleYalnız künye2012Scopus SJRQ4Phytoplankton growth and microzooplankton grazing in the Homa Lagoon (Izmir Bay, Turkey)
Banu, Kutlu
Phytoplankton growth and microzooplankton grazing were investigated at one station in the Homa Lagoon from February to January in 2006-2007. Our results showed significant seasonal variations in phytoplankton dynamics. Microzooplankton was mainly composed of dinoflagellates and tintinnid ciliates and nauplii. Microzooplankton grazing increased with increasing of temperature. Grazing rate was maximum levels in spring and summer. Microphytoplankton, which dominated the total algal biomass and production, were characterized by the proliferation of several chain-forming diatoms. Small heterotrophic flagellates and aloricate ciliates were the main controllers of phytoplankton. Phytoplankton represented a significant for micrograzers, which grazing represented 20-120% of diatom and cynabacteria algal production during 2006-2007. Microzooplankton has, however, a relatively high impact on microphytoplankton, as > 45% of microalgal production was consumed throughout the year. These results suggest that the low grazing was one of the factors contributing to the development of the spring bloom. All of seasonal bases, the phytoplankton production were grazed by microzooplankton in summer, autumn and winter-spring seasons have been changing between 20%-120%. The seasonal variation in the microzooplankton grazing pressure seems to result from the dominant size class of the phytoplankton community of this lagoon diatom and cynabacteria.
AtıfWoS1İncele