Sürdürülebilir Kalkınma Amaçları
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- MakaleYalnız künye2019Scopus SJRQ3Bio-functions of carvacrol-supplemented feeds on lipopolysaccharide-induced rainbow trout (Oncorhynchus mykiss Walbaum, 1792)
Kucukgul, A.; Gulsafak, I
To investigate the effects of carvacrol supplementation in rainbow trout induced by lipopolysaccharide (LPS), relative gene expressions levels of some pro-inflammatory cytokines and apoptosis markers in kidney and liver were measured. Fish with a mean weight of 44.71 +/- 1.33 g were studied using four different treatments with three replicates each. Inflammation and apoptosis were performed using LPS of Escherichia coli (25 mu g ml(-1)) except in the control group and only carvacrol (100 mu g ml(-1) diet) containing diets were fed to this group (CAR). The last group was the infected fish fed carvacrol supplemented diet (+CAR). Kidney and liver tissues were removed 3 days after to determine the levels of interleukin-1 beta (IL-1 beta), interferon gamma (INF-gamma), caspase 3 (Cas 3), caspase 8 (Cas 8) using Real-Time PCR analyses. IL-1 beta expressions of both kidney and liver was significantly decreased (12.9 and 2.14 fold, respectively) in LPS treated cells (p<0.05). While IFN-gamma expression was up regulated in kidney, it had down regulation in liver. LPS decreased both Cas 3 and 8 expressions in kidney but increased in liver. +CAR increased expressions of IL-1 beta and IFN-gamma compared with the control (up to 2-3 fold) in all tissues except caspase gene expressions that were similar in the control. In all tissues, IL-1 beta and IFN-gamma expressions increased in +CAR group, expect IFN-gamma in the kidney. However, Cas 3 and 8 expressions including apoptosis was induced by up-down regulations in all tissues compared to LPS-injected fish. The results showed that carvacrol had pro-inflammatory and apoptotic effects especially on liver tissue of LPS-induced inflammation model of Oncorhynchus mykiss.
AtıfWoS4OpenAlex3
İncele - MakaleYalnız künye2019Scopus SJRQ2Investigation of the anti-apoptotic activity of ozone therapy in rainbow trout macrophages infected with Yersinia ruckeri
Küçükgül, Azime; Küçükgül, Altuğ; Gönenci, Ramazan; Özsoy, Şule Yurdagül; Kutlu, Banu; Işgör, Mehmet Mustafa
Yersinia ruckeri is the causal agent of enteric redmouth (ERM) in trout. This study aimed to investigate the effectiveness of nontoxic ozone on Y. ruckeri-infected trout cells in in vitro conditions. Rainbow trout macrophages (RTS-11) were exposed to Y. ruckeri for 24 h after being incubated with the ozonized medium at nontoxic concentration. Effective concentrations of nontoxic ozone (3 gamma) and bacteria (1.5 x 108 cfu/mL) were determined by viability tests. The expression of caspases 1, 3, 8, and 12 was determined by real-time PCR and protein levels investigated by ELISA method. Apoptotic-necrotic cell ratios were determined by acridine orange and ethidium bromide staining. Bacterial stimulations of caspases (1, 3, 8, and 12) were suppressed by ozone mostly at the translational level. While bacterial infection increased the count of apoptotic and necrotic cells at different stages, ozone significantly reduced this condition. Obtained data indicate possible use of nontoxic ozone as a safe and effective treatment alternative for increasing host defenses and eliminating the infection in the case of yersiniosis in fish.
AtıfWoS1OpenAlex3
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