Sürdürülebilir Kalkınma Amaçları
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- MakaleAçık Erişim2019Comparative Micromorphological and Anatomical Investigations on the Subsection Distantes R. Bhattacharjee (Stachys L./Lamiaceae)
Açar, Mikail; Satil, Fatih
In this study; comparative anatomical and micromorphological studies were performed on the subsection Distantes of the Stachys L. genus (Lamiaceae). Also, it was determined distinctive anatomical characters for the taxa of subsection in the study. In anatomical studies, free-hand cross-sections and the paraffin embedding method was applied with the microtome. Micromorphological studies were performed in LM (Light Microscope) and SEM (Scanning Electron Microscope). In anatomical studies, besides of similarities in stem and leaf structures there were also some differences in taxa. In micromorphological studies, the trichome and nutlet characteristics were found to be useful characters on taxa and at species and subspecies level. As a result of the study, the presence of sclerenchyma like a bundle in the stem structure and the presence and types of glandular trichomes and ornamentation on the surface of nutlet, the presence of the wing and ventral structure in the nutlet were important characters that can be used to distinguish taxa. According to the numerical findings, trichome and nutlet micromorphological characters were more important than anatomical characters.
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İncele - MakaleAçık Erişim2016Scopus SJRQ1Chronic exposure of tilapia (Oreochromis niloticus) to iron oxide nanoparticles: Effects of particle morphology on accumulation, elimination, hematology and immune responses
Ates, Mehmet; Demir, Veysel; Arslan, Zikri; Kaya, Hasan; Yilmaz, Sevdan; Camas, Mustafa
Effects of chronic exposure to alpha and gamma iron oxide nanoparticles (alpha-Fe2O3 and gamma-Fe2O3 NPs) were investigated through exposure of tilapia (Oreochromis niloticus) to 0.1, 0.5 and 1.0 mg/L (9.2 x 10(-4), 4.6 x 10(-3) and 9.2 x 10(-3) mM) aqueous suspensions for 60 days. Fish were then transferred to NP-free freshwater and allowed to eliminate ingested NPs for 30 days. The organs, including gills, liver, kidney, intestine, brain, spleen, and muscle tissue of the fish were analyzed to determine the accumulation, physiological distribution and elimination of the Fe2O3 NPs. Largest accumulation occurred in spleen followed by intestine, kidney, liver, gills, brain and muscle tissue. Fish exposed to gamma-Fe2O3 NPs possessed significantly higher Fe in all organs. Accumulation in spleen was fast and independent of NP concentration reaching to maximum levels by the end of the first sampling period (30th day). Dissolved Fe levels in water were very negligible ranging at 4-6 mu g/L for alpha-Fe2O3 and 17-21 mu g/L for gamma-Fe2O3 NPs (for 1 mg/L suspensions). Despite that, Fe levels in gills and brain reflect more dissolved Fe accumulation from metastable gamma-Fe2O3 polymorph. Ingested NPs cleared from the organs completely within 30-day elimination period, except the liver and spleen. Liver contained about 31% of alpha- and 46% of gamma-Fe2O3, while spleen retained about 62% of alpha- and 35% of the gamma-polymorph. No significant disturbances were observed in hematological parameters, including hemoglobin, hematocrit, red blood cell and white blood cell counts (p > 0.05). Serum glucose (GLU) levels decreased in treatments exposed to 1.0 mg/L of gamma-Fe2O3 NPs at day 30 (p < 0.05). In contrast, GLU levels increased during the elimination period for 1.0 mg/L alpha-Fe2O3 NPs treatments (p < 0.05). Transient increases occurred in glutamic oxaloacetic transaminase (GOT), glutamic pyruvic transaminase (GPT), and lactate dehydrogenase (LDH). Serum Fe levels did not change during exposure (p > 0.05), but increased significantly within elimination period due to mobilization of ingested NPs from liver and spleen to blood. Though respiratory burst activity was not affected (p > 0.05), lysozyme activity (LA) was suppressed suggesting an immunosuppressive effects from both Fe2O3 NPs (p < 0.05). In contrast, myeloperoxidase (MPO) levels increased significantly in treatments exposed to alpha-Fe2O3 NPs (p < 0.05), and the effect from gamma-polymorph was marginal (p > 0.05). The results indicate that morphological differences of Fe2O3 NPs could induce differential uptake, assimilation and immunotoxic effects on O. niloticus under chronic exposure. (C) 2016 Elsevier B.V. All rights reserved.
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İncele - MakaleAçık Erişim2014Scopus SJRQ2Morphological, karyological features and pollen morphology of endemic Ebenus haussknechtii Bornm. ex Hub.-Mor. from Turkey: A traditional medicinal herb
Hayta, Sukru; Tasar, Neslihan; Cakilcioglu, Ugur; Gedik, Osman
In this study, morphological, morphometrical, karyological and detailed pollen morphology of Ebenus haussknechtii endemic to Turkey was studied for the first time. In the morphologic study, some morphological and morphometrical features of the plant were observed and compared with the published findings in the Flora of Turkey. Karyogram and idiograms of the plant were also prepared. The chromosome number of E. haussknechtii was determined as 2n = 14 and haploid karyotype formula 5 m + 1 sm + 1M. Metaphase chromosome length ranged from 2.16 to 1.93 mu m and the total haploid chromosome length was 17.90 mu m. The results of the light microscope investigation of the pollen revealed that the pollen grains were radially symmetrical, isopolar, tricolpate and prolate. Measurement of the reticulum heterobrachate indicated their ornamentation to be reticulate. (C) 2014 Elsevier GmbH. All rights reserved.
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