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- MakaleAçık Erişim2022Scopus SJRQ4The role of A268V exon-7 polymorphism of PPARA in development of axial spondyloarthritis; [Aksiyal spondiloartrit gelişiminde PPARA'nın A268V ekson-7 polimorfizminin rolü]
Akbulut, Ekrem; Yolbas, Servet; Ozgen, Metin
Objectives: Axial spondyloarthritis (axSpA) is a chronic inflammatory disease that mainly affects the axial skeleton. Peroxisome proliferator activated receptor alpha (PPARA) is an intracellular transcription factor, which play a role in inflammation and osteoblasting activity. This study is designed to investigate the relationship of NG_012204.2:p.Ala268Val polymorphism of PPARA with axSpA risk and its role in disease development. Methods: This study was conducted with 168 patients and 181 controls. Genotyping was done with MALDITOF. Gene expression level was analyzed by quantitative real time PCR (RT-qPCR). The protein homology models of PPARA were created with ProMod3. Ligand binding dynamics were tested using the AutoDock4 docking program. Statistical evaluations were made with SPSS (ver24) and GeneGlobe. Results: Our results showed that C>T polymorphism causing NG_012204.2:p.Ala268Val change was associated with disease risk (p=0.024) and T allele increased disease risk 1.7 times (95% CI=1.070-2.594). PPARA expression decreased (p<0.05) in individuals carrying the T allele. We determined that the ligand entry pocket was opened 1.1 Å in the polymorphic PPARA. Polymorphic change caused a decrease in the ligand binding affinity. Conclusions: Our results provide an important contribution to elucidating the development of axSpA and demonstrate the potential of PPARA as a marker for the diagnosis of axSpA.
- MakaleYalnız künye2017Scopus SJRQ2Investigation of blood antioxidant enzyme levels and glutathione peroxidase, catalase, and superoxide dismutase gene polymorphism in sheep with fluorosis
Ozbey, Ulku; Deger, Yeter; Yur, Fatmagul; Çambay, Zafer; Ozbeye, Gokben
Reactive oxygen species have been considered to play a significant part in the development of fluorosis, a critical public health issue in many parts of the world. OBJECTIVES: The aims of this study, conducted on two groups of sheep, with and without fluorosis, was (i) to determine the effect of fluorosis on antioxidant enzymes, such as superoxide dismutase (SOD), glutathione peroxidase (GSH-Px), catalase (CAT), and on malondialdehyde (MDA); and (ii) to investigate the relationship between the SOD, CAT, and GSH-Px genotypes and the antioxidant enzyme levels. METHODS: The two groups of sheep were: (i) 24 Akkaraman sheep with fluorosis living in Dogubeyazit province of Agri and (ii) 20 Akkaraman sheep with no signs of fluorosis living in Van. The MDA level and the antioxidant enzyme activities were identified spectrophometrically. The genotype characteristics were determined by the PCR-RFLP method using DNA extracted from blood. RESULTS: Compared to the control sheep without fluorosis, we found in the fluorotic sheep: (i) an increase in plasma MDA levels and GSH-Px-1 activity (p<0.05), and (ii) a decrease in SOD and CAT activities (p<0.01). There were significant differences found in the genotype and allele frequencies in both groups (p<0.01). CONCLUSIONS: Fluoride-induced oxidative stress affected the membrane structure resulting in elevated erythrocyte MDA levels and reduced enzyme activity of the enzymes SOD and CAT. According to our data, the gene polymorphisms show a tendency towards fluorosis for our local sheep population.
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