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- MakaleAçık Erişim2024Scopus SJRQ2Modeling the growth behavior of Salmonella spp. in grated carrots inoculated with different inoculum levels stored at various temperatures
Omac, Basri
The consumption of fresh and fresh-cut fruits and vegetables, such as carrots, has increased for the last decades for a healthy life and an adequate diet, but concerns regarding the microbial safety of them have been raised. The present study was conducted to develop predictive models for Salmonella spp. in grated carrots. The results showed that Salmonella spp. did not display growth at 5°C, but it grew in grated carrots at other temperatures (10, 15, 20, 25, and 37°C) for both inoculum levels. Also, the inoculum levels affected the growth of this pathogen in grated carrots when the storage temperatures ranged from 15 to 25°C. The theoretical minimum temperatures calculated using the Huang model were 3.48 and 5.79°C for inoculum levels of 101 and 102 CFU/g, respectively. The primary and secondary models performed well in terms of agreement between experimental and estimated values. Furthermore, compared to the Ratkowsky model, the theoretical minimum temperature was given a more reasonable value using the Huang model. The models developed in the present study will be a useful input for future quantitative microbial risk assessment to appraise the proliferation of Salmonella spp. in grated carrots throughout the production process, storage, and distribution.
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İncele - MakaleAçık Erişim2022Scopus SJRQ2Synthesis, characterization, and evaluation of antimicrobial performance of N-[3-(dimethylamino)propyl]methacrylamide based copolymer on pathogens
Pıhtılı, Güzin; Erecevit Sönmez, Pınar; Biryan, Fatih; Torğut, Gülben
In this study, poly{N-[3-(dimethylamino) propylmethacrylamide-co-acrylonitrile}; [P(DMAPMA-co-AcN)] copolymer was polymerized by free radical polymerization (FRP) in the presence of azobisisobutyronitrile (AIBN) initiator at 60°C. The structure of the synthesized polymer was characterized by the Fourier transform infrared (FT-IR), differential scanning calorimetry (DSC), and thermogravimetric analysis (TGA) methods. The bactericidal and fungicidal effects of the solutions prepared by adding methanol to this copolymer to prevent the growth of pathogenic bacteria, yeast, and fungi were evaluated by disk diffusion and broth microdilution method. Since the resistance of bacteria, yeast and fungi, which adversely affect human health, to antibiotics is an important issue, and there has not been any study on the antimicrobial properties of this copolymer, it has directed us to investigate its antimicrobial properties. According to antimicrobial susceptibility tests: The polymer solution showed a broad spectrum of antimicrobial action with 12.3–14.3 mm/inhibition zone diameter on Pseudomonas aeruginosa, Klepsiella pneumoniae, Enterococcus faecalis bacteria, Candida tropicalis, Epidermophyton floccosum yeast and fungi and showed a narrow spectrum of effect on inhibition of the growth of Candida albicans with 10.3 mm/inhibition zone diameter. No antimicrobial effects were observed on Staphylococcus aureus. The minimum inhibition concentration (MIC) value on the test microorganisms was determined as 125–31.25 μl. The data obtained prove that the polymer is in the same group as the interaction of bactericidal and fungicidal drugs. It is also important as a polymer with antimicrobial properties. This study aimed to produce a new polymer with antimicrobial properties. This polymer can be a new and yielding antimicrobial representative in different areas.
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