Sürdürülebilir Kalkınma Amaçları

• 24
• 10
• 10
• 4
• 1

• MakaleAçık Erişim2026Scopus SJRQ3/r/munzur/1356
  Changes in common carp (Cyprinus carpio) sperm quality and membrane lipid composition related to cryopreservation by Tribulus terrestris; [Alterações na qualidade do esperma e na composição lipídica da membrana da carpa comum (Cyprinus carpio) relacionadas à criopreservação por Tribulus terrestris]

  Ölçülü, Abdullatif; Özçiçek, Esin; Kocabaş, Filiz Kutluyer; Kocabaş, Mehmet; Kızak, Volkan; Yilmaz, Ökkeş

  Docosahexaenoic acid (DHA) was the major polyunsaturated fatty acid (PUFA) in the sperm plasma membrane, and the levels of PUFAs decreased in treated groups during the cryopreservation process. The supplementation of TT (Tribulus terrestris) to Cyprinus carpio sperm at 200 µg∙L-1 positively affected the maintenance of motility parameters and FAs levels after the freeze-thaw cycle, and TT can be used as an herbal medicine instead of synthetic pharmaceuticals to improve the productive performance in aquaculture.

  • 
  İncele
• MakaleYayıncıda açık erişim2026Scopus SJRQ2/r/munzur/1236
  Sperm cryopreservation of sex-reversed rainbow trout (Oncorhynchus mykiss): incorporation of amino acids and anti-oxidants to the extender media to improve the anti-oxidant system

  Kocabaş, Filiz Kutluyer; Kocabaş, Mehmet; Benzer, Fulya; Erişir, Mine

  Anti-oxidants are vital for protecting sperm and can mitigate the negative effects associated with cryopreservation. This study was conducted to evaluate the protective effects of adding anti-oxidants (vitamins and amino acids) to extender in sex-reversed rainbow trout (Oncorhynchus mykiss) sperm. The collected sperm was diluted at the ratio of 1: 5 by the extenders supplemented with different anti-oxidants including 1.00 mM of ascorbic acid and L-tryptophan, 2.00 mM of cysteine and α-tocopherol, and their combination. After dilution, the semen was aspirated into 0.50 mL straws, and the straws were placed on the tray, frozen for 10 min, and plunged into liquid nitrogen. Straws were thawed in a 30.00 ˚C water bath for 15 sec. The Sperm Class Analyzer System was used to evaluate sperm kinematics. The activity of anti-oxidant enzymes and lipid peroxidation were determined as oxidative stress indices. Our data indicated that the incorporation of anti-oxidants and amino acids increased sperm motility duration. The elevated activity of glutathione peroxidase, superoxide dismutase, and catalase in post-thaw samples indicates that the anti-oxidant system in sex-reversed rainbow trout sperm likely plays a crucial role in protecting membrane compounds from oxidation. In conclusion, the combination of 1.00 mM L-tryptophan and ascorbic acid to the extender media caused a prolonging effect in sperm motility after thawing and they have the potential to serve as effective agents for improving sperm cryosurvival.

  İncele
• MakaleYalnız künye2026Scopus SJRQ3/r/munzur/1203
  Characteristics of the growth rate, fatty acid profile, and shell content of the freshwater snail Viviparus contectus (Millet, 1813)

  Kocabaş, Filiz Kutluyer; Kizak, Volkan; Özçiçek, Esin; Kocabaş, Mehmet; Yilmaz, Ökkeş

  Freshwater snails are a nutritious food source, providing vital amino acids, minerals, and protein with low lipid levels. Hence, the adaptation ability, growth rate, fatty acid (FA) profile, and shell content of Viviparus contectus were determined. Biometric measurements were recorded for 6 months. Over the 6-month cultivation period, specific growth rates were recorded as 0.32% · day-1 for shell length and 0.92% · day-1 for body weight. The FA profile of the freshwater snail included a total of 32 compounds with saturated, monounsaturated, and polyunsaturated FAs represented by 13, 10, and 9 types, respectively. Monounsaturated fatty acids (MUFAs) exhibited the highest concentration, followed by saturated fatty acids (SFAs) s at 35.14% and polyunsaturated fatty acids (PUFAs) at 14.57%. Stearic acid (C18:0), erucic acid (C22:1 n-9), and arachidonic acid (C20:4 n-6) were determined as the most abundant FAs within the saturated, monounsaturated, and polyunsaturated FAs classes, respectively. The energy-dispersive X-ray spectroscopy spectra analysis revealed that the shell powder composition consisted of the elements, such as carbon, oxygen, calcium, iron, manganese, and silicium. Overall, V. contectus showed a clear increase in growth over the cultivation period, and future studies are needed to obtain the best culture conditions and intensive production of the species.

  • 
  • 
  İncele
• MakaleAçık Erişim2026Scopus SJRQ2/r/munzur/1176
  The Positive Effect of Antioxidants in Maintaining the Post-Cryopreserved Sperm Quality of Rainbow Trout Oncorhynchus mykiss (Walbaum, 1792)

  Handayani, Luvi S.; Rahayu, Sri R.; Razi, Nanda M.; Pane, Elya P.; Kocabas, Mehmet; Kocabas, Filiz Kutluyer; Hafizuddin, Hafizuddin; Fadli, Nur; Sulistiono, Sulistiono; Muchlisin, Zainal A.

  Rainbow trout aquaculture is challenging due to the restricted spawning seasons. Sperm cryopreservation technology can help ensure a successful spawning season, but its effectiveness is restricted by oxidative stress during freezing and thawing, which reduces the availability of male gametes. This study aimed to evaluate the effect of several types and concentrations of antioxidants on post-cryopreservation rainbow trout sperm quality. Subsequently, a factorial completely randomized design with three replications was applied, testing four types of antioxidants Ascorbic acid, Myo-inositol, Butylated hydroxytoluene (BHT), and Alpha-tocopherol at concentrations of 0, 20, 40, and 60 mM, respectively. The evaluation parameters included motility rate, sperm lifespan, and sperm motion characteristics using the Computer Assisted Sperm Analysis system. The results showed that the type and concentration of antioxidants significantly affected sperm motility and sperm lifespan (P<0.05). The treatment method carried out using 40 mM Ascorbic acid and 20 mM BHT led to the highest sperm motility of 46.67% and sperm lifespan of 34 seconds. Although both antioxidants showed similar effectiveness, BHT at a concentration of 20 mM is more recommended due to its lower toxicity and technical as well as economic efficiency considerations.

  • 
  İncele
• MakaleAçık Erişim2026Scopus SJRQ4/r/munzur/774
  INTEGRATED BIOLOGICAL TREATMENT AND ELECTROCOAGULATION FOR SLAUGHTERHOUSE WASTEWATER: A NOVEL APPROACH USING FRESHWATER SNAILS (Viviparus contactus, Planorbis rubrum, Melanoides tuberculata)

  Öğedey, Ayşenur; Kocabaş, Filiz Kutluyer; Kizak, Volkan; Kocabaş, Mehmet; Benzer, Fulya; Erişir, Mine; Öğedey, Hayrullah

  As a potential pollution source, slaughterhouse wastewater (SWW) with development of the meat production and processing sector is a threat in the world. Therefore, the determination of economical, favourable, cost-effective and proper treatment method is crucial for sustainable environmental protection. This study focused on the performance and efficiency of a two-stage process combining biological treatment (BT) with freshwater snails and electrocoagulation (EC) of SWW. Viviparus contectus, Planorbis rubrum, Melanoides tuberculata were used in primary treatment while secondary treatment was realized with EC process. The EC of SWW was performed for reducing both turbidity and chemical oxygen demand (COD) concentration to levels compliant with direct-discharge regulations. In addition, the stirring speed, current density, initial pH and reaction time were assessed for evaluation the optimum EC condition. Oxidative stress biomarkers and some antioxidants were assessed in freshwater snails exposed to SWW after 24 and 96 h. The best removal efficiencies of COD (91.43%) and turbidity (92.42%) were obtained at pH 5 and 20 min of operational time by P. rubrum. Notably, MDA (Malondialdehyde) was highest level in M. tuberculata compared to V. contectus and P. rubrum. Overall, removing organic and inorganic pollutants from SWW could be provided with the hybrid process BT/EC as a combining approach.

  İncele
• MakaleAçık Erişim2025/r/munzur/6216
  New Record of the Melanopsis buccinoidea (Olivier, 1801) from two streams of Mersin (Türkiye) with Evaluation Some Morphometric Parameters

  Kocabaş, Mehmet; Kutluyer Kocabaş, Filiz

  In this study, some biometric characteristics of the freshwater snail Melanopsis buccinoidea (Olivier, 1801) were determined in Koca Stream and Bayat Stream (Mersin, Türkiye). Weight (W) (g), shell length (SL) (mm), shell width (SW) (mm), aperture length (AL) and width (AW) (mm) measurements were made on the samples taken from different stations. Morphological relationships were determined by multivariate statistical evaluation. The highest values in terms of shell length and weight were determined to be 16.53±3.67 mm and 0.47±0.23 g, living in Koca Stream. The largest shell length measured in the samples was 21.93 mm and the aperture width was 10.62 mm. The results were analyzed using Principal Component Analysis (PCA), a versatile statistical technique employed to explore the relationships between variables and account for a significant portion of the dataset. Samples taken from different stations showed a strong relationship between aperture width and spir height.

  • 
  AtıfOpenAlex1

  İncele
• MakaleYalnız künye2025Scopus SJRQ4/r/munzur/4200
  The profile of fatty acids in the gametes of brook trout (Salvelinus fontinalis) ofduring the spawning period under culture conditions

  Kocabas, Mehmet; Kutluyer-Kocabas, Filiz; Basçinar, Nadir; Özçiçek, Esin; Kirmizikaya-Özmen, Gorkem; Yilmaz, Okkes

  The fertilization and subsequently embryo and larvae development are linked to gamete quality and FA (fatty acid) content is one of the specific factors affecting egg or sperm quality. Hence, FA profile of gametes (egg and sperm) was documented in brook trout (Salvelinus fontinalis) under cultivation. FAs were detected and measured using gas chromatography. These results indicated that a total of 26 and 22 FAs were determined in egg and sperm, respectively. The levels of PUFAs were determined in nearly identical quantities in both egg (47.57%) and sperm (47.53%). DHA (Docosahexaenoic acid), LA (Linoleic acid), EPA (Eicosapentaenoic acid) and ARA (Arachidonic acid) were predominant PUFAs for egg and sperm. Interestingly, vaccenic acid (C18:1 n-11) (24.07%) was major FAs in egg. In conclusion, the analysis of fatty acids in broodstock gametes contributes valuable insights into the dietary requirements necessary for effective aquaculture breeding programs.

  • 
  İncele
• MakaleYayıncıda açık erişim2025Scopus SJRQ2/r/munzur/2061
  Effect of pre-freezing on motility, viability and abnormality of Nile tilapia fish Oreochromis niloticus sperm post cryopreservation

  Muchlisin, Z.A.; Hasibuan, R.R.; Maulida, S.; Eriani, K.; Fadli, N.; Muhammadar, A.A.; Handayani, L.S.; Suharman, I.; Kocabas, M.; Kocabas, F.K.

  Nile tilapia Oreochromis niloticus is a popular freshwater fish that has been extensively and intensively cultured worldwide. However, cryopreservation of its sperm, especially pre-freezing procedure, has not been properly developed. Therefore, the study aimed to determine the best pre-freezing procedure for cryopreservation of Nile tilapia Oreochromis niloticus sperm. The completely randomized design with five treatments and four replications was employed in this study. The tested treatments were T1=4°C → 0°C →-4°C →-10°C →-79°C →-196°C, T2=4°C→ 0°C →-4°C →-10°C →-196°C, T3=4°C → 0°C →-4°C →-196°C, T4=4°C → 0°C →-196°C), and T5=4°C →-196°C, with a 10 min equilibration at each respective temperature. Furthermore, sperm was cryopreserved for two weeks in liquid nitrogen (-179oC). The results of the ANOVA test showed that pre-freezing had a significant effect on sperm motility, and viability (P<0.05), but had no considerable impact on sperm abnormality (P>0.05). Treatment T4 exhibited higher motility and viability, but these values were not significantly different from T3 and T5. Based on practical consideration, it was recommended to utilize the T5 pre-freezing procedures (4°C →-196°C) for cryopreservation of Nile tilapia sperm. Considering these results, Nile tilapia sperm can be directly cryopreserved into liquid nitrogen after equilibration at 4°C for 10 min.

  • 
  AtıfScopus1

  İncele
• MakaleAçık Erişim2025Scopus SJRQ3/r/munzur/1777
  l-Tryptophan improves the sperm quality of threatened Abant trout (Salmo abanticus) against glyphosate toxicity: impact on oxidative stress and DNA damage

  Kocabaş, Mehmet; Kocabaş, Filiz Kutluyer; Erişir, Mine; Benzer, Fulya; Arslan, Halil Ozancan; Keleş, Esin; Aksu, Önder

  The current study focused on the ameliorating impact of TRP (5 mM) on Salmo abanticus spermatozoa exposed to sub‑lethal concentrations of GLY (2.5, 5, and 10 mg/L). Sperm quality parameters, oxidative stress indices, and DNA damage were analyzed in the post‑exposure sperm. Our data reveal that GLY has an adverse effect on motility rate and duration, sperm kinetics parameters, antioxidant status and DNA damage, and triggers antioxidant defense mechanisms at the highest dose (10 mg/L) and it has insufficient therapeutic effect. Overall, GLY poses potential hazards to male reproductive cells and may adversely affect subsequent generations of natural populations.

  İncele
• MakaleAçık Erişim2025Scopus SJRQ4/r/munzur/1673
  The profile of fatty acids in the gametes of brook trout (Salvelinus fontinalis) during the spawning period under culture conditions; [Perfil de ácidos grasos en gametos de trucha de arro yo (Salvelinus fontinalis) cultivada en época de desove]

  Kocabaş, Mehmet; Kutluyer-Kocabaş, Filiz; Başçinar, Nadir; Özçiçek, Esin; Kirmizikaya-Özmen, Görkem; Yilmaz, Ökkeş

  The fertilization and subsequently embryo and larvae development are linked to gamete quality and FA (fatty acid) content is one of the specific factors affecting egg or sperm quality. Hence, FA profile of gametes (egg and sperm) was documented in brook trout (Sa lve linus fontinalis) under cultivation. FAs were detected and measured using gas chromatography. These results indicated that a total of 26 and 22 FAs were determined in egg and sperm, respectively. The levels of PUFAs were determined in nearly identical quantities in both egg (47.57%) and sperm (47.53%). DHA (Docosahexaenoic acid), LA (Linoleic acid), EPA (Eicosapentaenoic acid) and ARA (Arachidon ic acid) were predominant PUFAs for egg and sperm. Interestingly, vaccenic acid (C18:1 n-11) (24.07%) was major FAs in egg. In conclusion, the analysis of fatty acids in broodstock gametes contributes valuable insights into the dietary requirements necessary for effective aquaculture breeding programs.

  • 
  İncele