Sürdürülebilir Kalkınma Amaçları

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• MakaleAçık Erişim2025Scopus SJRQ3/r/munzur/1777
  l-Tryptophan improves the sperm quality of threatened Abant trout (Salmo abanticus) against glyphosate toxicity: impact on oxidative stress and DNA damage

  Kocabaş, Mehmet; Kocabaş, Filiz Kutluyer; Erişir, Mine; Benzer, Fulya; Arslan, Halil Ozancan; Keleş, Esin; Aksu, Önder

  The current study focused on the ameliorating impact of TRP (5 mM) on Salmo abanticus spermatozoa exposed to sub‑lethal concentrations of GLY (2.5, 5, and 10 mg/L). Sperm quality parameters, oxidative stress indices, and DNA damage were analyzed in the post‑exposure sperm. Our data reveal that GLY has an adverse effect on motility rate and duration, sperm kinetics parameters, antioxidant status and DNA damage, and triggers antioxidant defense mechanisms at the highest dose (10 mg/L) and it has insufficient therapeutic effect. Overall, GLY poses potential hazards to male reproductive cells and may adversely affect subsequent generations of natural populations.

  İncele
• MakaleAçık Erişim2024/r/munzur/6303
  Effect of Dietary Zinc on the Antioxidant Parameters of Juvenile Common Carp (Cyprinus carpio)

  Aksu, Önder; Harlıoğ, Muzaffer Mustafa; Mustafa, Shokri; Batool, Zahra

  Zinc (Zn) is an essential micro mineral needed for the proper growth and immune function of fish. This investigation was designed to examine the antioxidant role of a fortified diet with different Zn levels in the muscle and liver tissues of carp fry. A four-iso-nitrogen (35% crude protein) practical diet was produced that included graded levels of dietary zinc sulphate as a nutritional zinc resource in the fundamental diet supplemented with increased zinc levels (T1, control, 85 mg Zn kg-1, T2 105 mg Zn kg-1, T3, 125 mg Zn kg-1 and T4, 145 mg Zn kg-1). Even though the SOD and CAT analysis results did not show a linear increase in the increasing Zn ratio in the diets, higher values were obtained compared to the control groups. SOD highest values in T3 for the liver (0.713 ± 0.220 U/ml) and T1 for muscle (0.751 ± 0.144 U/ml), CAT values were highest in T2 for the liver (0.849 ± 0.115 nmol/dk/m) and T2 for muscle (1.059 ± 0.148 nmol/dk/m) was obtained. MDA values were completely higher for the muscle than for the control group, and for the liver, a lower value was obtained in the T2 trial group than in the control group (1.671 ± 0.230 µM). The results of the study showed that Zn contributed significantly to the nutrition of carp fish. It can be concluded that the findings of SOD and CAT analysis endorse the positive contributions of using 105 mg Zn in the diets to promote the antioxidant defense of juvenile carp fish.

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• MakaleAçık Erişim2024Scopus SJRQ1/r/munzur/1876
  World of Crayfish™: a web platform towards real-time global mapping of freshwater crayfish and their pathogens

  Ion, Mihaela C.; Bloomer, Caitlin C.; Bărăscu, Tudor I.; Oficialdegui, Francisco J.; Shoobs, Nathaniel F.; Williams, Bronwyn W.; Scheers, Kevin; Clavero, Miguel; Grandjean, Frédéric; Collas, Marc

  Freshwater crayfish are amongst the largest macroinvertebrates and play a keystone role in the ecosystems they occupy. Understanding the global distribution of these animals is often hindered due to a paucity of distributional data. Additionally, nonnative crayfish introductions are becoming more frequent, which can cause severe environmental and economic impacts. Management decisions related to crayfish and their habitats require accurate, up-to-date distribution data and mapping tools. Such data are currently patchily distributed with limited accessibility and are rarely up-to-date. To address these challenges, we developed a versatile e-portal to host distributional data of freshwater crayfish and their pathogens (using Aphanomyces astaci, the causative agent of the crayfish plague, as the most prominent example). Populated with expert data and operating in near real-time, World of Crayfish™ is a living, publicly available database providing worldwide distributional data sourced by experts in the field. The database offers open access to the data through specialized standard geospatial services (Web Map Service, Web Feature Service) enabling users to view, embed, and download customizable outputs for various applications. The platform is designed to support technical enhancements in the future, with the potential to eventually incorporate various additional features. This tool serves as a step forward towards a modern era of conservation planning and management of freshwater biodiversity. Copyright 2024 Ion et al.

  AtıfScopus41·OpenAlex36

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• MakaleYalnız künye2022/r/munzur/5844
  Enhancing motility of rainbow trout (Oncorhynchus mykiss) sperm by Tribulus terrestris extract supplementation

  Kocabaş, Mehmet; Kutluyer Kocabaş, Filiz; Aksu, Önder; Karakavuk, Emrah; Ölçülü, Abdullatif; Ince, Olcay Kaplan

  Tribulus terrestris (TT) is a famous traditional plant of family Zygophyllaceae and widely distributes around the world. TT has potential to elevate the testosterone, testosterone precursor and luteinizing hormone levels. In this study, trials were conducted to assessment the impact of Tribulus terrestris addition on sperm motility of rainbow trout (Oncorhynchus mykiss) for the first time. In the trial, we used to different concentrations [0 µg L-1 (Control), 200 µg L-1, 400 µg L-1, 600 µg L-1, 800 µg L-1 and 1000 µg L-1] of T. terrestris extract. Sperm motility characteristics and longevity were determined. In addition, protodioscin content of T. terrestris extract was assessed. The present study revealed that the presence of T. terrestris caused to increase in sperm motility. The increases in duration (49.00±3.61 s) and motility rate (91.67±2.89%) at 400 µg L-1 were statistically significant (p<0.05). Overall, addition of T. terrestris to activation solution can increase the sperm motility of rainbow trout.

  İncele
• MakaleAçık Erişim2022/r/munzur/5842
  Gölova Baraj Gölü (Sivas)’ndeki Siraz Balığı (Capoeta sieboldii Steindachner, 1864)’nın Yaş ve Bazı Büyüme Özellikleri

  Aksu, Önder; Yüngül, Mücahit; Altınterim, Başar

  Bu çalışmada, Temmuz 2020- Haziran 2021 tarihleri arasında Gölova Baraj Gölü’nden avlanan 200 adet (105 dişi, 95 erkek) siraz balığı (Capoeta sieboldii Steindachner, 1864)’nın yaş ve eşey kompozisyonu, boy ve ağırlık dağılımları, yaş-boy, yaş-ağırlık, boy-ağırlık ve boy-boy ilişkileri ile kondisyon faktörü incelenmiştir. Örneklerin %52,5’i dişi, %47,5’i erkek bireylerden oluşmaktadır. Dişi ve erkek bireylerin I-VIII yaş grupları arasında dağılım gösterdiği ve erkek/dişi oranının 1:0,91 olduğu belirlenmiştir. Örneklerin total boy ve ağırlık değerlerinin dişilerde 22,04-51,7 cm ve 96,0-1697,3 g; erkeklerde 21,9-49,9 cm ve 94,7-1387,3; tüm bireylerde 21,9-51,7 cm ve 94,7-1697,3 g arasında olduğu tespit edilmiştir. Aynı yaş grubundaki dişi ve erkek bireylerin istatistiksel olarak total boy ortalamaları arasındaki farklılığın VI ve VII. yaşlarda; ağırlık ortalamaları arasındaki farklılığın ise III, VI, VII ve VIII. yaşlarda önemli olduğu saptanmıştır (p<0,05). Örneklerin von Bertalanffy eşitliğine göre boyca ve ağırlıkça büyüme denklemleri dişilerde Lt=75,42[1-e-0,10(t+2,93)] ve Wt=5705,89[1-e-0,10(t+2,93)]3,3689; erkeklerde Lt=82,05[1-e-0,08(t+3,35)] ve Wt=8059,06[1-e-0,08(t+3,35)]3,4003; tüm bireylerde Lt=81,74[1-e-0,08(t+3,24)] ve Wt=7758,71[1-e-0,08(t+3,24)]3,3771 olarak hesaplanmıştır. Büyüme performans indeks (Ø') değerleri de dişilerde 2,757, erkeklerde 2,727 ve tüm bireylerde 2,746 olarak belirlendi. Boy-ağırlık ilişkisi dişilerde W=0,0027TL3,3689 (R2=0,9928), erkeklerde W=0,0025TL3,4003 (R2=0,9901) ve tüm bireylerde W=0,0027TL3,3771 (R2=0,9916) olarak tespit edilmiştir. Ortalama kondisyon faktörü değerlerinin tüm bireylerde 0,887-1,201 arasında değişim gösterdiği saptanmıştır. Eşey farkına bağlı olarak farklı boy tipleri arasında da kuvvetli bir ilişkinin olduğu gözlemlenmiştir

  İncele
• MakaleYalnız künye2022Scopus SJRQ3/r/munzur/2929
  Ascorbic acid ameliorated the sperm quality of rainbow trout (Oncorhynchus mykiss) against arsenic toxicity: Impact on oxidative stress, fertility ability and embryo development

  Kocabaş, Filiz Kutluyer; Kocabaş, Mehmet; Aksu, Önder; Çakir Sahilli, Yeliz

  Arsenic (As) is a heavy metal and aquatic pollutant and adversely impacts the reproduction of male fish. As a chain-breaking antioxidant, ascorbic acid (AA) has high water solubility and low toxicity. In this context, the current study was performed to assess the protective role of AA (1 mM) on the sperm cells of the rainbow trout (Oncorhynchus mykiss) exposed to sublethal concentrations of As (8, 16 and 32 mg/L). Sperm quality parameters were analyzed using a sperm class analyzer system. Lipid peroxidation and antioxidant enzyme levels were used as indicators of oxidative stress. The fertilization, eyeing and hatching rates were determined as gamete markers. Reduced sperm quality parameters and fertility capacity resulted from in vitro exposure to As (P < 0.05). The oxidative stress in sperm cells increased after As exposure (P < 0.05). The presence of AA improved sperm movement parameters and fertility potential (P < 0.05). Overall, AA had a positive effect on oxidative stress and fertility ability against As toxicity and AA supplementation ameliorated detrimental effects of As in sperm cells.

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  AtıfScopus11·WoS9·OpenAlex5

  İncele
• MakaleAçık Erişim2022Scopus SJRQ3/r/munzur/2829
  Effects of kefir supplementation on body antioxidant-systems, haemolymph heat-shock protein 70 and trypsin, in the crayfish Astacus leptodactylus Eschscholtz (Decapoda, Astacidea)

  Aksu, Önder; Türkoǧlu, Semra; Can, Erkan; Erişir, Mine; Kocabaş, Mehmet; Kayim, Murathan; Benzer, Fulya

  This study was designed to investigate the effects of dietary kefir on trypsin, heat shock protein 70 (HSP70) as well as the oxidant and antioxidant status of the freshwater crayfish, Astacus leptodactylus. The experiment was performed with the four following treatments: Control group, D1, D2 and D3 (kefir supplemented diet 2, 4, 8 g/kg of crayfish body mass, respectively). Crayfish were stocked into 12 fibreglass tanks (three replicates per treatment) and fed for 45 days. Reduced glutathione (GSH), glutathione peroxidase (GSH-Px), malondialdehyde (MDA) and catalase (CAT) in hepatopancreas and muscle were analysed to determine the status of the oxidant/antioxidant balance. In addition, HSP70 and trypsin were measured in haemolymph. Our results indicated that the activity of GSH-Px, MDA and GSH levels decreased in hepatopancreas ( p > 0.05) and muscle tissues ( p < 0.05) with increasing kefir concentration, while CAT activity increased ( p < 0.05). Trypsin and HSP70 insignificantly decreased in haemolymph.

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  AtıfScopus1·WoS1·OpenAlex1

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• MakaleYalnız künye2022Scopus SJRQ2/r/munzur/2606
  Sperm quality and oxidative stress in chub Squalius orientalis and Padanian barbel Barbus plebejus (Teleostei: Cyprinidae) after in vitro exposure to low doses of bisphenol A

  Kutluyer, Filiz; Çakir Sahilli, Yeliz; Kocabaş, Mehmet; Aksu, Önder

  In an aquatic environment, the presence of Bisphenol A (BPA) adversely affects reproduction, biology, behavior, gonads, and early larval development of fish due to being endocrine-disrupting compound. In addition, the detected concentration of BPA in water bodies is reported to be higher than 0.41 μg/L. As an alternative tool, sperm cells are used in toxicological assays for the reliable and practical assessment. For these reasons, we examined the effects of in vitro exposure of BPA on sperm quality of chub Squalius oriantalis and Padanian barbel Barbus plebejus. Spermatozoa were exposed to lower concentrations (0, 0.5, 1.25, 2.5, and 5 µg/L) of BPA for 2 h. The enzymatic activities [glutathione peroxidase (GSH-Px), catalase (CAT), and superoxide dismutase (SOD)] and lipid peroxidation (MDA) were evaluated in spermatozoa. The results demonstrated that BPA exposure significantly decreased activities of SOD and GSH-Px but increased CAT activity and lipid peroxidation (MDA). Compared to control, the percentage and duration of sperm motility significantly decreased. Overall, spermatozoa clearly showed the sensitivity to lower concentrations of BPA.

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  AtıfScopus16·WoS17·OpenAlex14

  İncele
• MakaleAçık Erişim2022Scopus SJRQ1/r/munzur/2441
  Aroclor 1254 impairs sperm quality, fertilization ability, and embryo development of rainbow trout (Oncorhynchus mykiss)

  Kocabaş, Mehmet; Kocabaş, Filiz Kutluyer; Aksu, Önder; Benzer, Fulya; Erişir, Mine; Sahilli, Yeliz Çakir

  The polychlorinated biphenyls (PCBs) in aquatic environment adversely affect non-target organisms, including fish. Especially, the male reproduction and next generation can be damaged through high exposure to these pollutants. Hence, the sperm cells were exposed to sublethal concentrations of Aroclor 1254 (0, 1, 5, 10, or 25 mg/l) for 4 h. The sperm quality parameters were analyzed by SCA (Sperm Class Analyzer). The fertility, eyeing, and hatching rates were determined as gamete markers. Lipid peroxidation (malondialdehyde-MDA), glutathione (GSH), and antioxidant enzymes [superoxide dismutase (SOD), glutathione peroxidase (GPx), and catalase (CAT)] were measured for determination of oxidative stress. Our results showed that Aroclor 1254 negatively affected the motility rate and duration, fertilization rate, embryogenesis, and hatching and also triggered antioxidant defense mechanisms at the highest concentration (25 mg L−1). Furthermore, linear speed (VSL), linearity index (LIN), and amplitude lateral head (ALH) were significantly changed after exposure to 25 mg L−1, and the lowest concentrations (1 and 10 mg L−1) did not significantly affect the motility and fertilizing capacity. The embryogenesis and hatching were significantly affected by sperm exposure to 1, 10, and 25 mg L−1 of Aroclor 1254. Consequently, Aroclor 1254 causes potential hazards in male germ cells, and the exposure of sperm cells to pollutants can adversely affect next generation of wild populations.

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  AtıfScopus6·WoS5·OpenAlex4

  İncele
• MakaleYalnız künye2021Scopus SJRQ2/r/munzur/3038
  Cellular responses of spirlin Alburnoides bipunctatus spermatozoa exposed to Bisphenol A: Biochemical response and sperm quality alterations evaluation

  Kocabaş, Mehmet; Kutluyer, Filiz; Çakir Sahilli, Yeliz; Aksu, Önder

  Plastics are one of the global environmental problems owing to their highest production. Bisphenol A (BPA) is used in the manufacturing of plastics including polycarbonate, polyvinyl chloride and resins. In particular, aquatic ecosystems are adversely affected by the occurrence of micropollutants. In addition, BPA as a typical endocrine-disrupting chemical (EDC) seriously influences androgenic activity and reproduction of aquatic organisms, particularly fish as non-target organism. In toxicological assays, sperm cells can be used as a potential tool for reliable and practical assessment. For these reasons, sperm cells were exposed to lower concentrations (0 µg/L, 0.5 µg/L, 1.25 µg/L, 2.5 µg/L and 5 µg/L) of BPA and the study focused on BPA-induced oxidative stress. The enzymatic [glutathione peroxidase (GSH-Px), catalase (CAT) and superoxide dismutase (SOD)] activities and lipid peroxidation (MDA) were analyzed in spermatozoa. The study findings showed that the activities of SOD and GSH-Px were significantly decreased by BPA but lipid peroxidation (MDA) and CAT acticity increased. Notably, the percentage and duration of motile sperm cells decreased. Collectively, spermatozoa clearly showed the sensitivity even at extremely low concentrations. This is first report to assess the impact of BPA on sperm cells of spirlin Alburnoides bipunctatus.

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  AtıfScopus6·WoS6·OpenAlex5

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