Sürdürülebilir Kalkınma Amaçları
Yayınlar
- MakaleAçık Erişim2025Scopus SJRQ3l-Tryptophan improves the sperm quality of threatened Abant trout (Salmo abanticus) against glyphosate toxicity: impact on oxidative stress and DNA damage
Kocabaş, Mehmet; Kocabaş, Filiz Kutluyer; Erişir, Mine; Benzer, Fulya; Arslan, Halil Ozancan; Keleş, Esin; Aksu, Önder
The current study focused on the ameliorating impact of TRP (5 mM) on Salmo abanticus spermatozoa exposed to sub‑lethal concentrations of GLY (2.5, 5, and 10 mg/L). Sperm quality parameters, oxidative stress indices, and DNA damage were analyzed in the post‑exposure sperm. Our data reveal that GLY has an adverse effect on motility rate and duration, sperm kinetics parameters, antioxidant status and DNA damage, and triggers antioxidant defense mechanisms at the highest dose (10 mg/L) and it has insufficient therapeutic effect. Overall, GLY poses potential hazards to male reproductive cells and may adversely affect subsequent generations of natural populations.
- MakaleAçık Erişim2024Effect of Dietary Zinc on the Antioxidant Parameters of Juvenile Common Carp (Cyprinus carpio)
Aksu, Önder; Harlıoğ, Muzaffer Mustafa; Mustafa, Shokri; Batool, Zahra
Zinc (Zn) is an essential micro mineral needed for the proper growth and immune function of fish. This investigation was designed to examine the antioxidant role of a fortified diet with different Zn levels in the muscle and liver tissues of carp fry. A four-iso-nitrogen (35% crude protein) practical diet was produced that included graded levels of dietary zinc sulphate as a nutritional zinc resource in the fundamental diet supplemented with increased zinc levels (T1, control, 85 mg Zn kg-1, T2 105 mg Zn kg-1, T3, 125 mg Zn kg-1 and T4, 145 mg Zn kg-1). Even though the SOD and CAT analysis results did not show a linear increase in the increasing Zn ratio in the diets, higher values were obtained compared to the control groups. SOD highest values in T3 for the liver (0.713 ± 0.220 U/ml) and T1 for muscle (0.751 ± 0.144 U/ml), CAT values were highest in T2 for the liver (0.849 ± 0.115 nmol/dk/m) and T2 for muscle (1.059 ± 0.148 nmol/dk/m) was obtained. MDA values were completely higher for the muscle than for the control group, and for the liver, a lower value was obtained in the T2 trial group than in the control group (1.671 ± 0.230 µM). The results of the study showed that Zn contributed significantly to the nutrition of carp fish. It can be concluded that the findings of SOD and CAT analysis endorse the positive contributions of using 105 mg Zn in the diets to promote the antioxidant defense of juvenile carp fish.
- MakaleAçık Erişim2024Scopus SJRQ1World of Crayfish™: a web platform towards real-time global mapping of freshwater crayfish and their pathogens
Ion, Mihaela C.; Bloomer, Caitlin C.; Bărăscu, Tudor I.; Oficialdegui, Francisco J.; Shoobs, Nathaniel F.; Williams, Bronwyn W.; Scheers, Kevin; Clavero, Miguel; Grandjean, Frédéric; Collas, Marc
Freshwater crayfish are amongst the largest macroinvertebrates and play a keystone role in the ecosystems they occupy. Understanding the global distribution of these animals is often hindered due to a paucity of distributional data. Additionally, nonnative crayfish introductions are becoming more frequent, which can cause severe environmental and economic impacts. Management decisions related to crayfish and their habitats require accurate, up-to-date distribution data and mapping tools. Such data are currently patchily distributed with limited accessibility and are rarely up-to-date. To address these challenges, we developed a versatile e-portal to host distributional data of freshwater crayfish and their pathogens (using Aphanomyces astaci, the causative agent of the crayfish plague, as the most prominent example). Populated with expert data and operating in near real-time, World of Crayfish™ is a living, publicly available database providing worldwide distributional data sourced by experts in the field. The database offers open access to the data through specialized standard geospatial services (Web Map Service, Web Feature Service) enabling users to view, embed, and download customizable outputs for various applications. The platform is designed to support technical enhancements in the future, with the potential to eventually incorporate various additional features. This tool serves as a step forward towards a modern era of conservation planning and management of freshwater biodiversity. Copyright 2024 Ion et al.
AtıfScopus41OpenAlex36
İncele - MakaleYalnız künye2022Enhancing motility of rainbow trout (Oncorhynchus mykiss) sperm by Tribulus terrestris extract supplementation
Kocabaş, Mehmet; Kutluyer Kocabaş, Filiz; Aksu, Önder; Karakavuk, Emrah; Ölçülü, Abdullatif; Ince, Olcay Kaplan
Tribulus terrestris (TT) is a famous traditional plant of family Zygophyllaceae and widely distributes around the world. TT has potential to elevate the testosterone, testosterone precursor and luteinizing hormone levels. In this study, trials were conducted to assessment the impact of Tribulus terrestris addition on sperm motility of rainbow trout (Oncorhynchus mykiss) for the first time. In the trial, we used to different concentrations [0 µg L-1 (Control), 200 µg L-1, 400 µg L-1, 600 µg L-1, 800 µg L-1 and 1000 µg L-1] of T. terrestris extract. Sperm motility characteristics and longevity were determined. In addition, protodioscin content of T. terrestris extract was assessed. The present study revealed that the presence of T. terrestris caused to increase in sperm motility. The increases in duration (49.00±3.61 s) and motility rate (91.67±2.89%) at 400 µg L-1 were statistically significant (p<0.05). Overall, addition of T. terrestris to activation solution can increase the sperm motility of rainbow trout.
- MakaleAçık Erişim2022Gölova Baraj Gölü (Sivas)’ndeki Siraz Balığı (Capoeta sieboldii Steindachner, 1864)’nın Yaş ve Bazı Büyüme Özellikleri
Aksu, Önder; Yüngül, Mücahit; Altınterim, Başar
Bu çalışmada, Temmuz 2020- Haziran 2021 tarihleri arasında Gölova Baraj Gölü’nden avlanan 200 adet (105 dişi, 95 erkek) siraz balığı (Capoeta sieboldii Steindachner, 1864)’nın yaş ve eşey kompozisyonu, boy ve ağırlık dağılımları, yaş-boy, yaş-ağırlık, boy-ağırlık ve boy-boy ilişkileri ile kondisyon faktörü incelenmiştir. Örneklerin %52,5’i dişi, %47,5’i erkek bireylerden oluşmaktadır. Dişi ve erkek bireylerin I-VIII yaş grupları arasında dağılım gösterdiği ve erkek/dişi oranının 1:0,91 olduğu belirlenmiştir. Örneklerin total boy ve ağırlık değerlerinin dişilerde 22,04-51,7 cm ve 96,0-1697,3 g; erkeklerde 21,9-49,9 cm ve 94,7-1387,3; tüm bireylerde 21,9-51,7 cm ve 94,7-1697,3 g arasında olduğu tespit edilmiştir. Aynı yaş grubundaki dişi ve erkek bireylerin istatistiksel olarak total boy ortalamaları arasındaki farklılığın VI ve VII. yaşlarda; ağırlık ortalamaları arasındaki farklılığın ise III, VI, VII ve VIII. yaşlarda önemli olduğu saptanmıştır (p<0,05). Örneklerin von Bertalanffy eşitliğine göre boyca ve ağırlıkça büyüme denklemleri dişilerde Lt=75,42[1-e-0,10(t+2,93)] ve Wt=5705,89[1-e-0,10(t+2,93)]3,3689; erkeklerde Lt=82,05[1-e-0,08(t+3,35)] ve Wt=8059,06[1-e-0,08(t+3,35)]3,4003; tüm bireylerde Lt=81,74[1-e-0,08(t+3,24)] ve Wt=7758,71[1-e-0,08(t+3,24)]3,3771 olarak hesaplanmıştır. Büyüme performans indeks (Ø') değerleri de dişilerde 2,757, erkeklerde 2,727 ve tüm bireylerde 2,746 olarak belirlendi. Boy-ağırlık ilişkisi dişilerde W=0,0027TL3,3689 (R2=0,9928), erkeklerde W=0,0025TL3,4003 (R2=0,9901) ve tüm bireylerde W=0,0027TL3,3771 (R2=0,9916) olarak tespit edilmiştir. Ortalama kondisyon faktörü değerlerinin tüm bireylerde 0,887-1,201 arasında değişim gösterdiği saptanmıştır. Eşey farkına bağlı olarak farklı boy tipleri arasında da kuvvetli bir ilişkinin olduğu gözlemlenmiştir
- MakaleYalnız künye2022Scopus SJRQ3Ascorbic acid ameliorated the sperm quality of rainbow trout (Oncorhynchus mykiss) against arsenic toxicity: Impact on oxidative stress, fertility ability and embryo development
Kocabaş, Filiz Kutluyer; Kocabaş, Mehmet; Aksu, Önder; Çakir Sahilli, Yeliz
Arsenic (As) is a heavy metal and aquatic pollutant and adversely impacts the reproduction of male fish. As a chain-breaking antioxidant, ascorbic acid (AA) has high water solubility and low toxicity. In this context, the current study was performed to assess the protective role of AA (1 mM) on the sperm cells of the rainbow trout (Oncorhynchus mykiss) exposed to sublethal concentrations of As (8, 16 and 32 mg/L). Sperm quality parameters were analyzed using a sperm class analyzer system. Lipid peroxidation and antioxidant enzyme levels were used as indicators of oxidative stress. The fertilization, eyeing and hatching rates were determined as gamete markers. Reduced sperm quality parameters and fertility capacity resulted from in vitro exposure to As (P < 0.05). The oxidative stress in sperm cells increased after As exposure (P < 0.05). The presence of AA improved sperm movement parameters and fertility potential (P < 0.05). Overall, AA had a positive effect on oxidative stress and fertility ability against As toxicity and AA supplementation ameliorated detrimental effects of As in sperm cells.
AtıfScopus11WoS9OpenAlex5İncele - MakaleAçık Erişim2022Scopus SJRQ3Effects of kefir supplementation on body antioxidant-systems, haemolymph heat-shock protein 70 and trypsin, in the crayfish Astacus leptodactylus Eschscholtz (Decapoda, Astacidea)
Aksu, Önder; Türkoǧlu, Semra; Can, Erkan; Erişir, Mine; Kocabaş, Mehmet; Kayim, Murathan; Benzer, Fulya
This study was designed to investigate the effects of dietary kefir on trypsin, heat shock protein 70 (HSP70) as well as the oxidant and antioxidant status of the freshwater crayfish, Astacus leptodactylus. The experiment was performed with the four following treatments: Control group, D1, D2 and D3 (kefir supplemented diet 2, 4, 8 g/kg of crayfish body mass, respectively). Crayfish were stocked into 12 fibreglass tanks (three replicates per treatment) and fed for 45 days. Reduced glutathione (GSH), glutathione peroxidase (GSH-Px), malondialdehyde (MDA) and catalase (CAT) in hepatopancreas and muscle were analysed to determine the status of the oxidant/antioxidant balance. In addition, HSP70 and trypsin were measured in haemolymph. Our results indicated that the activity of GSH-Px, MDA and GSH levels decreased in hepatopancreas ( p > 0.05) and muscle tissues ( p < 0.05) with increasing kefir concentration, while CAT activity increased ( p < 0.05). Trypsin and HSP70 insignificantly decreased in haemolymph.
AtıfScopus1WoS1OpenAlex1İncele - MakaleYalnız künye2022Scopus SJRQ2Sperm quality and oxidative stress in chub Squalius orientalis and Padanian barbel Barbus plebejus (Teleostei: Cyprinidae) after in vitro exposure to low doses of bisphenol A
Kutluyer, Filiz; Çakir Sahilli, Yeliz; Kocabaş, Mehmet; Aksu, Önder
In an aquatic environment, the presence of Bisphenol A (BPA) adversely affects reproduction, biology, behavior, gonads, and early larval development of fish due to being endocrine-disrupting compound. In addition, the detected concentration of BPA in water bodies is reported to be higher than 0.41 μg/L. As an alternative tool, sperm cells are used in toxicological assays for the reliable and practical assessment. For these reasons, we examined the effects of in vitro exposure of BPA on sperm quality of chub Squalius oriantalis and Padanian barbel Barbus plebejus. Spermatozoa were exposed to lower concentrations (0, 0.5, 1.25, 2.5, and 5 µg/L) of BPA for 2 h. The enzymatic activities [glutathione peroxidase (GSH-Px), catalase (CAT), and superoxide dismutase (SOD)] and lipid peroxidation (MDA) were evaluated in spermatozoa. The results demonstrated that BPA exposure significantly decreased activities of SOD and GSH-Px but increased CAT activity and lipid peroxidation (MDA). Compared to control, the percentage and duration of sperm motility significantly decreased. Overall, spermatozoa clearly showed the sensitivity to lower concentrations of BPA.
AtıfScopus16WoS17OpenAlex14İncele - MakaleAçık Erişim2022Scopus SJRQ1Aroclor 1254 impairs sperm quality, fertilization ability, and embryo development of rainbow trout (Oncorhynchus mykiss)
Kocabaş, Mehmet; Kocabaş, Filiz Kutluyer; Aksu, Önder; Benzer, Fulya; Erişir, Mine; Sahilli, Yeliz Çakir
The polychlorinated biphenyls (PCBs) in aquatic environment adversely affect non-target organisms, including fish. Especially, the male reproduction and next generation can be damaged through high exposure to these pollutants. Hence, the sperm cells were exposed to sublethal concentrations of Aroclor 1254 (0, 1, 5, 10, or 25 mg/l) for 4 h. The sperm quality parameters were analyzed by SCA (Sperm Class Analyzer). The fertility, eyeing, and hatching rates were determined as gamete markers. Lipid peroxidation (malondialdehyde-MDA), glutathione (GSH), and antioxidant enzymes [superoxide dismutase (SOD), glutathione peroxidase (GPx), and catalase (CAT)] were measured for determination of oxidative stress. Our results showed that Aroclor 1254 negatively affected the motility rate and duration, fertilization rate, embryogenesis, and hatching and also triggered antioxidant defense mechanisms at the highest concentration (25 mg L−1). Furthermore, linear speed (VSL), linearity index (LIN), and amplitude lateral head (ALH) were significantly changed after exposure to 25 mg L−1, and the lowest concentrations (1 and 10 mg L−1) did not significantly affect the motility and fertilizing capacity. The embryogenesis and hatching were significantly affected by sperm exposure to 1, 10, and 25 mg L−1 of Aroclor 1254. Consequently, Aroclor 1254 causes potential hazards in male germ cells, and the exposure of sperm cells to pollutants can adversely affect next generation of wild populations.
AtıfScopus6WoS5OpenAlex4İncele - MakaleYalnız künye2021Scopus SJRQ2Cellular responses of spirlin Alburnoides bipunctatus spermatozoa exposed to Bisphenol A: Biochemical response and sperm quality alterations evaluation
Kocabaş, Mehmet; Kutluyer, Filiz; Çakir Sahilli, Yeliz; Aksu, Önder
Plastics are one of the global environmental problems owing to their highest production. Bisphenol A (BPA) is used in the manufacturing of plastics including polycarbonate, polyvinyl chloride and resins. In particular, aquatic ecosystems are adversely affected by the occurrence of micropollutants. In addition, BPA as a typical endocrine-disrupting chemical (EDC) seriously influences androgenic activity and reproduction of aquatic organisms, particularly fish as non-target organism. In toxicological assays, sperm cells can be used as a potential tool for reliable and practical assessment. For these reasons, sperm cells were exposed to lower concentrations (0 µg/L, 0.5 µg/L, 1.25 µg/L, 2.5 µg/L and 5 µg/L) of BPA and the study focused on BPA-induced oxidative stress. The enzymatic [glutathione peroxidase (GSH-Px), catalase (CAT) and superoxide dismutase (SOD)] activities and lipid peroxidation (MDA) were analyzed in spermatozoa. The study findings showed that the activities of SOD and GSH-Px were significantly decreased by BPA but lipid peroxidation (MDA) and CAT acticity increased. Notably, the percentage and duration of motile sperm cells decreased. Collectively, spermatozoa clearly showed the sensitivity even at extremely low concentrations. This is first report to assess the impact of BPA on sperm cells of spirlin Alburnoides bipunctatus.
AtıfScopus6WoS6OpenAlex5İncele - MakaleAçık Erişim2019Influence of Macerated and Cold Press Wheat Germ (Triticum vulgare) Oils Added at Different Rates to Trout Feed on The Feed Rate (FCR), Hematological Parameters and NBT (Nitroblue tetrazolium) Values of Rainbow Trout (Oncorhynchus mykiss)
Aksu, Önder; Altınterim, Başar
In this study, 2% of macerated and cold press wheat germ oils were added to the feeds of rainbow trout (average weight of 39.8 ± 4 g). At the end of the study, growth rate, blood parameters and NBT (Nitro blue tetrazolium) level of rainbow trout were determined. Feed Conversion Ratios (FCR) of groups were determined as macerated: 1.12, cold press: 1.53, control: 1.11. Statistically significant differences were found in red blood cell (RBC), hemoglobin (HGB), hematocrit (HCT), white blood cell (WBC), platelet (PLT) values (p<0.05). There was no statistically significant difference between Nitroblue Tetrazolium (NBT) levels (p>0.05).
AtıfTR Dizin1
İncele - MakaleAçık Erişim2019Effect of Macerated Tomato (Lycopersicon esculentum) and Carrot (Daucus carota) Oils on Hematological Parameters of Rainbow Trout (Oncorhynchus mykiss) at High Stocking Density
Aksu, Önder; Altınterim, Başar
In this study, 2% of macerated tomato (Lycopersicon esculentum) and carrot (Daucus carota) oils were added to the feeds of rainbow trout with an average weight of 35.88 ± 1.97 g. The experiment was continued for 21 days and the samples were also measured for changes in the hematological parameters. Statistically, significant differences were found in LYM, RBC, HGB, HCT, MCH and NBT values (p<0.05). There was no statistically significant difference other blood parameters levels (p>0.05). It was determined that both macerated carrot and macerated tomato oils stimulated to the non-specific immune system and erythropoiesis at high stocking density. Especially, it was occurred that macerated carrot oil (MCO) more effective than macerated tomato oil (MTO).
AtıfOpenAlex3TR Dizin1İncele - MakaleAçık Erişim2019Congo Red Effects on Detoxification and Metabolic Enzymes in Rainbow Trout (Oncorhynchus mykiss Walbaum, 1792)
Danabaş, Durali; Yildirim, Nuran Cikcikoglu; Yildirim, Numan; Aksu, Önder
The aim of this study was to evaluate the activities of the biotransformation enzymes (Glutatyon STransferase (GST) and cytochrome P450 (CYP1A1)) and metabolic enzyme (Lactatedehydrogenase-LDH) in the liver of rainbow trout (Oncorhynchus mykiss) after 24 and 48 hourexposure to 0.5, 1.0 and, 2.0 mg l-1 of Congo red (CR). Enzyme activities were determined by usingcommercial kits with ELISA method. Congo red altered the activities of GST, CYP1A, and LDHenzymes in liver tissue of O. mykiss in a dose-dependent manner. The statistical differences in GSTactivities among the groups for 24 and 48 h were significant, but, LDH activities were significantfor only 24 h. Exposure duration of CR didn’t affect the biochemical response of rainbow trout.Thus, CR exposure changed the biotransformation and metabolic enzymes, and the changes of theseenzymes activities may be used as a potential bioindicator of the CR exposure.
- MakaleAçık Erişim2019Masere sarımsak (Allium sativum Limne) ve Tunceli sarımsağı (Allium tuncelianum Kollman) yağlarının yoğun stoklanmış gökkuşağı alabalıklarının (Oncorhynchus mykiss W.) bazı kan parametrelerine ve NBT (Nitroblue Tetrazolium) seviyelerine etkileri
Aksu, Önder; Altınterim, Başar
Bu çalışmada, % 2 oranında masere sarımsak (Allium sativum L.) ve Tunceli sarımsağı(Allium tuncelianum Koll.) yağları, ortalama ağırlıkları: 39.0 ± 1.64 gr. olan gökkuşağıalabalıklarının yemlerine ilave edilmiştir. Çalışmanın sonunda, gökkuşağıalabalıklarının kan parametreleri ve NBT (Nitroblue Tetrazolium) seviyeleri tespitedilmiştir. Sarımsak uygulaması yapılan gruplarda, MCH, P-LCR, PCT, PLT ve MPVparametrelerinde kontrol ve stres gruplarına göre istatistiksel olarak farklılıkbulunmuştur (P<0.05). WBC, LYM, MID, RBC, HGB, HCT, MCV, MCHC, RDW-SD,RDW-CV ve PDW, değerleri arasında bir farklılık görülmemiştir (P> 0.05). Sarımsakuygulamaları sonucu NBT seviyeleri önemli derecede azalma göstermiştir (P<0.05). Budurum sarımsağın hücrelerdeki oksidatif stresi azalttığını göstermektedir.
AtıfOpenAlex8TR Dizin5İncele - MakaleAçık Erişim2019Scopus SJRQ2Improvement of sperm motility of Oncorhynchus mykiss and Salvelinus fontinalis by L-tryptophan
Kocabaş, Mehmet; Kutluyer, Filiz; Ertekin, Özlem; Aksu, Önder; Başçınar, Nadir
Experiments were designed to test the influence of L-tryptophan supplementation of the extender on the sperm motility parameters and bacterial flora of brook trout (Salvelinus fontinalis) and rainbow trout (Oncorhynchus mykiss). The extender containing 0.3 M glucose in 10% Dimethyl sulfoxide (DMSO) was supplemented with 0, 0.5, 1, 2, and 4 mM L-tryptophan. Sperm samples stored for up to 12 days at 4°C for brook trout were motile while motility was not observed after six days of storage for rainbow trout. Diluted sperm samples were spread-plated onto Plate Count Agar (PCA) (Total Bacteria Count), Rose Bengal Agar (RBC) (Yeast-Mold Count), Violet Red Bile Dextrose (VRBD) (Enterobacteriaceae count) and Mannitol Salt Agar (MSA) (Micrococcus/Staphylococcus count) and counts were performed in duplicate and sampling occurred on day 0, 2, 4, 6, and 12 of cold storage. L-tryptophan supplementation of the extender yielded a positive effect, significantly prolonging sperm motility in S. fontinalis and O. mykiss compared to the control group (p < 0.05). The ideal results were obtained above a concentration of 0.5 mM for both species. Total bacteria count in fresh sperm (undiluted samples) was not detectable and not detected in sperm samples treated with different L-tryptophan concentrations. Yeast-mold, Enterobacteriaceae and Micrococcus/Staphylococcus were not detected from fresh and treated sperm brook trout and rainbow trout. On the basis of the results, L-tryptophan-based extender is useful for maintaining sperm quality in short-term storage. L-tryptophan had a negative effect on the bacterial flora. The results of the current study encourages further studies related to long-term storage and reproduction management. Abbreviations: DMSO: dimethyl sulfoxide; PCA: plate count agar; RBC: rose Bengal agar; VRBD: violet red bile dextrose; MSA: mannitol salt agar; SCA: sperm class analyzer; CFU: colony-forming unit.
AtıfScopus13WoS14OpenAlex13İncele - MakaleAçık Erişim2018Scopus SJRQ4Effects of Different Plant Oils Having Different Oxygen Radical Absorbance Capacity (ORAC) on Hematological Parameters of Rainbow Trout (Oncorhynchus mykiss) at High Stocking Density
Aksu, Önder; Altınterim, Başar; Kutluyer Kocabaş, Filiz
In this study, macerated oils of different plant [sunflower (Helianthus annuus), onion (Allium cepa), garlic (Allium sativum), basil (Ocimum basilicum), opium poppy (Papaver somniferum), ginger (Zingiber officinale), turmeric (Curcuma longa)] were obtained. The study was conducted at a total of 7 groups, including the control group (sunflower oil), in 30 liter aerated tanks without water circulation during a week. Twenty rainbow trout per tank (14.6±1.0 g) were stocked at high density. Macerated oils were applied to the skin of the fish along the lateral line on the first day. For determination of effects of macerated oils on water and fish, oxidationreduction potential (ORP) of water and hematological parameters [granulocyte (GRAN), white blood cell (WBC), red blood cell (RBC), hemoglobin (HGB), mean corpuscular hemoglobin (MCH), red cell distribution width (RDW-SD), mean platelet volume (MPV), platelet distribution width (PDW), platelet large cell ratio (P-LCR)] were examined in fish. The results indicated that decreases were determined in ORP level of water. It was determined that the highest survival rate was in the application of basil oil. At the end of 7 days, the highest WBC, LYM, HGB, MCV, MCH, MPV values were determined in the application of ginger oil (ORAC: 28.811). It was determined that the highest RBC value was in the application of turmeric oil (ORAC: 159.277), the highest GRAN value was in the application of poppy oil (ORAK: 481), the highest HCT, PLT values was in the application of basil oil (ORAC: 67.553). At the end of the study, it was determined that the macerated oil application to lateral line induced blood parameters.
AtıfOpenAlex3TR Dizin7İncele - MakaleAçık Erişim2018Scopus SJRQ4Effects of green tea (Camellia sinensis) Oil on Feed Utilization and Hametological Parameters of Rainbow Trout (Oncorhynchus mykiss)
Aksu, Önder; Öztürk, Ekrem; Kutluyer, Filiz; Altınterim, Başar
In the study, experiments were conducted to determine effects of green tea oil (Camellia sinensis) on the growth rate, feed utilization and hematological parameters of rainbow trout (Oncorhynchus mykiss). Fish (76.25±2.75 g) were fed diets supplemented with different concentrations of green tea oil (%0.25, %0.5, %1.0). Weight gain (WG), feed conversion ratio (BDI) and some blood parameters were determined in the rainbow trout. It was determined that the values of WBC, GRAN, HGB, HCT, MCH, MCHC, PLT, PDW and PCT were increased with respect to control group at the end of 42 days feeding with green tea oil. However, groups treated with green tea oil stimulated megakaryocytes (increased PLC-R levels), which also increased platelet production. Feed evaluation ratio varied between 1.83-4.53. At the end of the experiment, it was determined that the feed evaluation ratio decreased with the increase of the amount of green tea oil in the groups. It is thought that the bioactive substances in the lipophilic property of green tea oil provide thermogenic stimulation in fishes by synergistic effect, which causes weight loss. Besides, it was determined that antioxidants such as hematopoiesis and catechins in hematopoietic organs of green tea oil act on the granulocyte cell group.
AtıfOpenAlex10TR Dizin8İncele - Proje2018Metal ve Metal Oksit Nanopartiküller ile KuantumNoktacıklarının Fitoplankton ve ZooplanktonOrganizmalarda Etkilerinin Araştırılması
Taştan, Burcu Ertit; Aksu, Önder; Kutlu, Banu; Danabaş, Durali; Ates, Mehmet; Arslan, Zikri
Nanopartikül (NP) içeren ürünler; tıbbi cihazlar, elektronik, enerji üretimi ve depolanması, sağlık bilimleri ve gıda sektörleri gibi birçok alanda kullanılmaktadır. NP?lerin büyüleyen özelliklerine rağmen, insan ve çevre sağlığına etkileri konusunda çok az şey bilinmektedir. Çünkü nanomateryaller metallerden, metal oksitlerlerden ve yarı-iletken maddelere kadar geniş bir yelpazeye sahiptir. Sucul sistemler ve besin zincirini oluşturan organizmalar antropojenik faaliyetlerden salınan NP?lerin etkileri sebebiyle risk altındadırlar. Bu çalışmada, tatlısu ve deniz suyu ortamında yaşayan birincil üretici fitoplankton türlerinden Selenastrum capricornutum ve Nannochloropsis oculata ile birincil tüketici zooplankton türlerinden Daphnia magna ve Artemia salina metal ve metal oksit NP?lere maruz bırakılarak nanotoksisite testleri gerçekleştirilmiştir. Çalışmada kullanılan metal ve metal oksit NP?leri (Al, Al2O3, Cu; CuO, Fe2O3, Zn ve ZnO) ile laboratuvarda sentezlenen Ag-NP?leri, CdSe ve PbSe kuantum noktacıklarının karakterizasyonu TEM, DLS, Zeta Potansiyeli, XRD, SEM, FT-IR ve UV görünüm spektroskopisi yöntemleriyle gerçekleştirilmiştir. Organizmalarda NP?lere bağlı birikim ve eliminasyon oranın belirlenmesi ICP-MS analiziyle yapılmıştır. Fitoplankton ve zooplanktonlarda NP etkileri faz kontrast mikroskobuyla görüntülenmiştir. Akut toksisite etkileri 24, 48 ve 72 saatlik periyotlar boyunca fitoplankton populasyonunun büyümesi flurometreyle in-vivo chl-a incelenmiştir. Zooplanktonlardaki toksik etkiler, mortalite ve oksidatif stres belirteçlerinden glutatyon (GSH) ve tiyobarbitürat reaktif maddeler (TBARS) testleriyle incelenmiştir. Sonuçlar NP?lerin organizmalar üzerindeki farklı etkilerini göstermekle birlikte, NP?lerin birikim ve eliminasyon oranlarında farklılıklar olduğunu göstermiştir. Organizmadaki birikim NP konsantrasyonuyla artış göstermiştir. NP?lerin metal iyon salınımları, tatlısu ve deniz suyu ortamında farklı olmakla birlikte, herbir NP?ün farklı iyon salınımı gösterdiği gözlenmiştir. NP?lere maruz bırakılan fitoplanktonların yüzeyleri NP?ler ile sarıldığı gözlemlenmiştir. Benzer sonuçlar zooplanktonlarda da gözlemlenmiştir. Oksidatif stres analizlerinde, TBARS değeri D. magna bireylerinde artış gösterirken bazı NP?ler A. salina?da azalmasına neden olmuştur. Benzer şekilde GSH seviyesinde azalma ve artmalar meydana gelmiştir. Organizma tarafından alınan NP?ler bir üst tüketici için tehlike oluşturmaktadır. Ayrıca fitoplanktonların yüzeylerinin NP?lerle kaplanması, üst besin zincirini oluşturan tüm organizmalar için risk oluşturmaktadır
- MakaleAçık Erişim2018Scopus SJRQ2Observations of crayfish plague infections in commercially important narrow-clawed crayfish populations in Turkey
Kokko, Harri; Harlioglu, Muzaffer M.; Aydin, Hamdi; Makkonen, Jenny; Gökmen, Gökhan; Aksu, Önder; Jussila, Japo
We studied the presence of possible Aphanomyces astaci infections in eight Turkish narrow-clawed crayfish (Astacus leptodactylus) populations by analyzing the prevalence and genotypes of the disease agent A. astaci. The qPCR analyses revealed A. astaci infection in seven of the studied eight populations, with the agent level A2 or higher. The agent levels among the infected populations varied from A0 to A5, i.e., from negative to high level of infection, based on qPCR ranking. Based on the sequencing of the chitinase gene and the mitochondrial ribosomal rnnS and rnnL subunits, we detected both A (As) and B (PsI) haplogroups of A. astaci in our samples, with each of the studied populations being carriers of only one haplotype. The results confirm previous detections of A. astaci in Turkish narrow-clawed crayfish populations and reveal, that both A and B haplogroup A. astaci carriers exist widely in A. leptodactylus populations of Turkey.
AtıfScopus28WoS29OpenAlex23İncele - MakaleYalnız künye2017The relationships between length-weight, and meat yield of freshwater crayfish (Astacus leptodactylus Eschscholtz, 1823) of Aktaş Lake
Aksu, Önder; Kurt Kaya, Gülderen
In this study, determination of morphometric analysis and meat yields were aimed in male and female crayfish caught from Aktaş Lake, where commercial crayfish hunting is prohibited. The length distribution of 157 crayfish (nmale= 81 and nfemale= 76) was examined in the study. It was determined that there was a strong linear relationship between carapace length and weight of both male and female crayfish (r ♂ = 0.962, r ♀= 0.976, r ♂ + ♀ = 0.9712). The "b values" obtained as a result of regression analysis showed that male crayfish had positive weight gain, and female crayfish had negative weight gain, and all individuals had positive allometric weight gain (b♂= 3.041, b♀= 2.954 ve b♂+♀=3.0167). It was shown that there was a linear relationship between abdominal meat quantities and carapace lengths of male and female crayfish. Abdominal meat yield, chelae meat yield and total meat yield in male crayfish was 10.03%, 4.60% and 14.63%, respectively, abdominal meat yield, chelae meat yield and total meat yield in female crayfish was 11.59%, 2.73% and 14.32%, respectively
AtıfTR Dizin2İncele