Congo Red Effects on Detoxification and Metabolic Enzymes in Rainbow Trout (Oncorhynchus mykiss Walbaum, 1792)

The aim of this study was to evaluate the activities of the biotransformation enzymes (Glutatyon STransferase (GST) and cytochrome P450 (CYP1A1)) and metabolic enzyme (Lactatedehydrogenase-LDH) in the liver of rainbow trout (Oncorhynchus mykiss) after 24 and 48 hourexposure to 0.5, 1.0 and, 2.0 mg l-1 of Congo red (CR). Enzyme activities were determined by usingcommercial kits with ELISA method. Congo red altered the activities of GST, CYP1A, and LDHenzymes in liver tissue of O. mykiss in a dose-dependent manner. The statistical differences in GSTactivities among the groups for 24 and 48 h were significant, but, LDH activities were significantfor only 24 h. Exposure duration of CR didn’t affect the biochemical response of rainbow trout.Thus, CR exposure changed the biotransformation and metabolic enzymes, and the changes of theseenzymes activities may be used as a potential bioindicator of the CR exposure.