Sürdürülebilir Kalkınma Amaçları
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- MakaleAçık Erişim2024Scopus SJRQ3Toxicity assessment of advanced biological wastewater treatment plant effluent by integrated biomarker response in zebra mussels (Dreissenapolymorpha)
Serdar, Osman; Ketenalp, Zozan; Yildirim, Nuran Cikcikoglu
In this study, zebra mussels (Dreissena polymorpha) were exposed to advanced biological wastewater treatment plant effluent (ABWTPE) for 96 h. At the end of the 96th hour, antioxidant parameters such as superoxide dismutase (SOD), catalase (CAT), glutathione peroxidase (GPX), glutathione S-transferase (GST), malondialdehyde (MDA) and glutathione (GSH) were examined. The objective of the study was to identify biomarkers that are useful for assessing the potential toxic effects of ABWTPE in freshwater environments. We observed an increase in GPX, SOD activity and MDA levels, and a decrease in CAT, GST activity and GSH levels. The results obtained in our study showed that the measured biochemical parameters (GSH, MDA, SOD, CAT, GPX and GST) are useful biomarkers in determining the possible toxicity of ABWTPE in aquatic environments.
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İncele - MakaleAçık Erişim2024Scopus SJRQ1Determination of oxidative stress responses caused by aluminum oxide (γ-Al2O3 and α-Al2O3) nanoparticles in Gammarus pulex
Serdar, Osman; Aydin, Ayşe Nur; Çimen, Isıl Canan Çiçek
The rapid growth in the use of aluminum oxide nanoparticles (Al2O3 NPs) in various fields such as medicine, pharmacy, cosmetics industries and engineering, and the fact that these NPs and their wastes mix with the aquatic environment and damage the aquatic ecosystem, affect the organisms in the water, enter the food chain and reach humans is a major problem is cause for concern. The aim of this study is to investigate the oxidative stress caused by two separate forms of aluminum oxide, γ-Al2O3 and α-Al2O3, in Gammarus pulex, which is a good indicator species, with biochemical parameters. For this purpose, G. pulex was exposed to different concentrations (0, 10, 20, 40 ppm) of γ-Al2O3 and α-Al2O3 separately. The experiments were carried out for 24 and 96 h by creating 3 repeated experimental groups consisting of 4 groups. For biomarker analysis, superoxide dismutase (SOD), catalase (CAT) activities and glutathione (GSH) and thiobarbituric acid (TBARS) levels were performed using an ELISA kit. As a result of the in experimental study, it was observed that both nanoparticles affected oxidative stress and antioxidant parameters after 96 h compared to the control group. Increases in SOD activity were observed, γ-Al2O3 caused a decrease in CAT activity at 24 h, and α- Al2O3 caused increases in CAT activity at 96 h. Decreases in GSH levels and increases in TBARS levels have been observed.
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İncele - MakaleYalnız künye2024Scopus SJRQ3The RSM-CCD Optimization of Fenton Process for Industrial Wastewater Treatment and Determination of Treatment Efficiency Using Some Biochemical Responses in Dreissena polymorpha
Tanyol, Mehtap; Serdar, Osman; Ketenalp, Zozan; Yildirim, Nuran Cikcikoglu; Erguven, Gokhan Onder; Yildirim, Numan
The study investigated antioxidant biomarkers in Dreissena polymorpha to evaluate the efficiency of Fenton process (FP) in Organized Industrial Zone Wastewater (OIZW) treatment. Antioxidant enzymatic activities, including superoxide dismutase (SOD) and catalase (CAT), glutathione peroxidase (GPX), non-enzymatic antioxidants (GSH) and the indicator of lipid peroxidation (TBARS) were measured in D. polymorpha in response to treated OIZW by FP. The performance of the FP has been optimized with response surface methodology (RSM). The optimum conditions (reaction time, and H2O2 and Fe2+ concentration) for OIZW by the FP process were determined. The results showed that the highest COD removal efficiency (83.94 %) was obtained when as Fe2+ concentration=402.35 mg/L, H2O2 concentration=4100.30 mg/L, and reaction time=10.10 min (desirability=1.000). SOD and CAT enzyme was decreased in the 1/25 diluted OIZW group compared to control group. CAT and SOD enzyme activities were increased after treatment compared to control groups. No statistically significant changes were observed in GSH levels and GPx activities. TBARS levels were increased 1/25 diluted OIZW exposure groups compared to the control group. TBARS levels were decreased after treatment. The results revealed that antioxidant parameters are useful biomarkers for determining the treatment efficiency of the FP.
AtıfScopus2WoS1OpenAlex2İncele - MakaleAçık Erişim2024Scopus SJRQ3sThe Bioremediation of Industrial Effluents by Phanerochaete Chrysosporium: The COD, TOC Removal Efficiency and Biochemical Assessment for Dreissena Polymorpha
Serdar, Osman; Yıldırım, Numan; Onder Erguven, Gokhan; Ketenalp, Zozan; Cikcikoglu Yildirim, Nuran; Durmus, Barbaros
Bioremediation is one of the cheapest and easy method for biological treatment for most kind of industial wastewater. Bioremediation potential of the Phanerochaete chrysosporium for industrial wastewater from Industrial Zone Wastewater plant in Mardin, Turkey, was evaluated. The chemical oxygen demand (COD) and total organic carbon (TOC) reduction efficiencies of P. chrysoporium of on wastewater studied using biomarkers such as catalase (CAT), superoxide dismutase (SOD), glutathione peroxidase (GPx), Thiobarbituric Acid (TBARS) and reduced glutathione (GSH) levels in Dreissena polymorpha. The level of TBARS, a marker of lipid peroxidation was found to be high because of wastewater exposure after treatment. TBARS levels decreased (from 20.2 to 31.6 and from 20.8 to 37.5). GSH levels (from 52.3 to 15.9), CAT levels (from, 119.7 to 15.3 and from 91.1 to 13.4) SOD levels (from 3.8 to 0.9 and from 3.4 to 1.2) and GPX activity (from 177.2 to 104.4 and 174.2 to 100.5) decreased in the wastewater exposure groups during 24 and 96th hour compared to the control groups. GSH levels, CAT, SOD and GPX activity increased after treatment by P. chrysosporium. Results indicate that industrial wastewater caused oxidative stress in Dreissena polymorpha. The findings revealed that the highest removal efficiency for COD and TOC, at 86.3 % and 80.3 % respectively, occurred in the 1/20 diluted medium. P. chrysosporium has proven to be effective in the bioremediation of wastewater from the Industrial Zone Changes in biochemical parameters before and after bioremediation showed that antioxidant parameters such as CAT, SOD, GPx activities and GSH and TBARS levels can be used as biomarker to evaluate bioremediation efficiency.
AtıfScopus4WoS2OpenAlex4İncele - MakaleAçık Erişim2023Length-Weight, Length-Length Relationships and Condition Factor of Squalius cephalus (Linnaeus, 1758) in the Munzur River
Serdar, Osman; Ozcan, Ebru Ifakat
In this study; some features were investigated such as length-weight, length-length relationships and condition factor of Squalius cephalus (Linnaeus, 1758) were collected between 2019-2021 from Munzur River. 49 were female and 59 were male; female/male ratio was found to be 1/1.20. Total length and weight ranged between 8.2-24.1 cm and 6.8-148.6 g for female; 8.9-24.3 cm and 7.3-156.8 g for male. The length-weight relationship of S. cephalus was found W = 0.0079L3,1229 (R² = 0.965) in females, W = 0.0074L3.1536 (R² = 0.971) in males and W= 0.0076L3.1407 (R² = 0.969) in all individuals. It was determined that the b value was not statistically different from 3 in female, male and all individuals (t-test; p>0.05). The growth of the S. cephalus was determined to be isometric (b=3) in the Munzur River. The relationship between total, fork and standard lengths was determined for female, male and all individuals were quite strong (P<0.001; R2>0.927). Condition factor of S. cephalus varies between 0.827-1.438 in females and 0.826-1.517 in males from Munzur River.
- MakaleYalnız künye2023Scopus SJRQ2The potential ameliorative impacts of rare earth element cerium in dimethoate induced toxicity in freshwater mussel Dreissena polymorpha
Serdar, Osman; Ketenalp, Zozan; Cikcikoglu Yildirim, Nuran
The purpose of this study was to measure the antioxidant parameters to examine the potential ameliorative impacts of the rare earth element (REE) cerium (Ce) in dimethoate (DM) induced toxicity in Dreissena polymorpha. Lethal concentration 50 (LC50) of DM for 96 h was measured. The antioxidant effect of Ce was investigated by exposure of 1/2 of the LC50 value of DM together with 4, 20 and, 100 mg/L of Ce to D. polymorpha. The responses of the biomarkers were determined by measuring the superoxide dismutase (SOD), catalase (CAT), glutathione peroxidase (GPx), glutathione (GSH) and malondialdehyde (MDA) levels. SOD, CAT, GPX and GSH levels were reduced (p < 0.05), while the MDA levels increased significantly (p < 0.05) in mussels exposed to DM. When DM was exposured together with Ce, it was observed that the MDA levels decreased, SOD, CAT, GPX and GSH levels increased during 24 and 96 h. The results of our study showed that Ce exerted a strong antioxidant effect on toxicity induced by DM. It has been shown that Ce may have a compensatory effect with the positive change in the antioxidant response of D. polymorpha to DM. This study is the first report about Ce and insecticide effects in bivalves.
AtıfScopus1WoS1OpenAlex1İncele - MakaleYalnız künye2023Scopus SJRQ4Individual and Combined Toxicity of Polycyclic Aromatic Hydrocarbons Phenanthrene and Fluoranthene in Freshwater Amphipod Gammarus pulex (L., 1758) (Amphipoda: Gammaridae)
Yildirim, Nuran Cikcikoglu; Serdar, Osman; Derman, Taner
The combined toxicity of polycyclic aromatic hydrocarbons on freshwater amphipod Gammarus pulex was investigated. The 14-day LC50 values of fluoranthene, phenanthrene and their combination for G. pulex were determined as 5.88±0.23, 8.31±0.57 and 7.00±0.33 mg/L, respectively. To evaluate the single and combined toxicity, sublethal concentrations of fluoranthene, phenanthrene and their combination were exposed to G. pulex for 14 days. Acetylcholinesterase, glutathione-s-transferase and cytochrome P450 1A1 enzymes were measured as biomarkers. Acetylcholinesterase activities showed significant inhibition at the end of 7 and 14 days. No statistically significant change was observed in cytochrome P450 1A1 levels at the end of the 7th day, but a significant increase was detected at the end of the 14th day. A significant inhibition was observed in glutathione-s-transferase activities at the end of the 7th and 14th days. Statistically significant changes were observed in all parameters when the application times are compared. It was determined that G. pulex exposed to different concentrations of phenanthrene and fluoranthene individually and in combination caused significant changes in activites of the all measured enzymes. It was concluded that the cytochrome P450 1A1, glutathione-s-transferase and acetylcholinesterase enzymes in G. pulex are useful biochemical markers for the determination of polycyclic aromatic hydrocarbon toxicity.
AtıfScopus3İncele - MakaleAçık Erişim2023Scopus SJRQ4Single and combined effects of dimethoate and malathion on oxidative stress biomarkers in the non-target freshwater mussel Dreissena polymorpha
Serdar, Osman; Yildirim, Numan; Yildirim, Nuran Cikcikoglu
This study evaluates the single and combined effects of dimethoate (DI) and malathion (MA) on oxidative stress biomarkers in the freshwater mussel Dreissena polymorpha, estimating the potential harm of these pesticides on aquatic ecosystems. Superoxide dismutase (SOD) and catalase (CAT) activities, and glutathione (GSH) and lipid peroxidation (TBARS) levels were determined in D. polymorpha exposed to sublethal concentrations of DI, MA and a combination of the two during 24 and 96 h periods. The results showed that the GSH levels were decreased but the TBARS levels were increased in all the exposure groups after 24 and 96 h compared to the control. It was observed that SOD activity decreased but CAT activity increased in all the DI exposure groups after 24 h compared to the control. At the end of 96 hours, it was observed that CAT and SOD activities increased again in some exposure groups compared to the control. Exposure time also had an effect on biomarkers in different levels. According to the results, the cytotoxicity of DI and MA combination depended on their concentrations. DI or/ and MA, in sub-lethal concentrations, induced oxidative damages in D. polymorpha. Combined exposure of the pesticides can alter their toxicity and may be evidence of increased toxicity and oxidative stress.
AtıfScopus3WoS3OpenAlex2İncele - MakaleYalnız künye2023Scopus SJRQ4Neurotoxic Effect of New Generation Insecticide with Flubendiamide Active Ingredient in Gammarus pulex (Linnaeus, 1758) (Amphipoda: Gammaridae) at Different Temperatures
Söylemez, Hilal; Serdar, Osman
This study aimed to determine the relationship between temperature and neurotoxicity of a new generation insecticide, the active ingredient of which is flubendiamide, on non-target aquatic organisms (Gammarus pulex (L., 1758)). Firstly, LC50 values of flubendiamide on G. pulex were determined at 16, 18 and 20 °C as 256.83 ± 19.59 µgL-1, 169.38 ± 34.17 µgL-1 and 155.84 ± 17.22 µgL-1, respectively. We found that the acute toxicity sensitivity of the test organism to the pesticide containing flubendiamide increased with increasing temperature. Secondly, G. pulex was exposed to non-lethal concentrations of flubendiamide pesticide for 96 hours at the above temperatures. Finally, the changes in acetylcholinesterase (AChE) enzyme activity were determined for each temperature using ELISA kits. The exposure time between the 24th and 96th h at the same temperature was found to be statistically insignificant (p>0.05). It was determined that the inhibition value of AChE enzyme activity on the test organism of the pesticide with active ingredient flubendiamide increased depending on the temperature increase. Considering the changes in AChE enzyme activities in G. pulex at different temperatures against flubendiamide insecticide, it was concluded that it can be used as a useful biomarker.
AtıfScopus1İncele - MakaleAçık Erişim2022Determination of Letal Concentrations (LC50) of Cyfluthrın, Dimethoate Insecticides on Gammarus pulex (L., 1758)
Serdar, Osman; Aydin, Rahmi; Aydın, Ayşe Nur
In this study, Gammarus pulex (L., 1758) individuals obtained from Tunceli Munzur Stream were exposed to different concentrations of two insecticides containing cyfluthrin and dimethoate active ingredients, and LC50 values were determined from acute toxicity tests. During the study, parameters such as water temperature, pH, and dissolved oxygen were constantly controlled to ensure that they did not change. In this study, experiments were carried out by placing 0.5 liters of water in 1-liter glass aquariums. 10 G. pulex individuals were used for each concentration group. To determine the LC50 value, the mobility of the living things was observed and recorded in 24-hour periods. G. pulex, which lost its motility, was removed from the aquarium and excluded from the study. The study was carried out in 3 replications and the LC50 value for dimethoate was determined as 170.51 ± 8.15 µg/l, while the LC50 value for cyfluthrin was determined as 0.800 ± 0.12 µg/l.
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İncele - MakaleAçık Erişim2022Zebra Midye (Dreissena polymorpha)'de Malathionun Akut Toksisitesi (LC50)’nin Belirlenmesi
Serdar, Osman; Ödün, Neşe Akçanal
Sunulan çalışmada, zebra midye (Dreissena polymorpha) bireyleri, LC50 değerlerinin belirlenmesi için, konsantrasyon düzeyleri farklı olan, malathion etken maddesini içeren pestisitin bu organizma üzerindeki akut toksik etkisi araştırılmıştır. Bu amaçla aralık belirleme ve deneysel tasarım kurgulanmıştır. Tüm deneyler (aralık belirleme dahil) 2 litre hacimli cam kaplar içerisine 1 litre su katılarak yapılmıştır. Her akvaryum içerisine, 10’ar adet D. polymorpha bireyi eklenerek, deneme çalışması hazırlanmıştır. LC50 değerinin belirlenmesi için gözlem periyodu olarak, 24 saatlik zaman dilimindeki D. polymorpha bireylerinin canlılık durumları incelenerek kayıt altına alınmıştır. Canlılık özelliğini yitiren D. polymorpha bireyleri deneme ortamından çıkarılmıştır. Bu şekilde 3 tekrarlı olarak yürütülen çalışmada, malathion etken maddeli pestisitin D. polymorpha için LC50 değeri 120,11 ± 19,32 mg/l olarak belirlenmiştir.
AtıfOpenAlex1İncele - MakaleAçık Erişim2022Dimethoate ve Malathion Pestisitlerinin Dreissena polymorpha (Pallas, 1771) Üzerindeki Kombine Etkisinin Sitokrom P450 ve Asetilkolinesteraz Kullanılarak Belirlenmesi
Serdar, Osman; Yildirim, Nuran Cikcikoglu; Yildirim, Numan
Bu çalışmada, Dreissena polymorpha’da organofosforlu dimethoate ve malathion etken maddeli insektisit karışımlarının toksisitesini ortaya çıkarmak için sitokrom P450 (CYP1A1) ve Asetilkolinesteraz (AchE) aktiviteleri ölçülmüştür. Dimethoate ve malathion'un öldürücü konsantrasyonu (LC50) 96 saat maruziyet sonunda 40,82±2,54 mg/L olarak hesaplanmıştır. D. polymorpha dimethoate ve malathionun üç subletal konsantrasyonuna (LC50 değerine 1/16, 1/8 ve 1/4 oranla) 24 saat ve 96 saat boyunca maruz bırakılmıştır. CYP1A1 ve AChE seviyeleri, ticari kit kullanılarak mikroplaka okuyucuda ölçülmüştür. AChE aktivitelerinin kontrol grubuna göre 96 saat sonra tüm maruziyet gruplarında düştüğü gözlenmiştir. Maruziyet süreleri karşılaştırıldığında 96. saat sonunda enzim aktivitelerinde istatistiksel olarak anlamlı bir değişiklik olmadığı belirlenmiştir. Tüm gruplarda 24 saatin sonunda CYP1A1 seviyelerinde istatistiksel olarak anlamlı bir değişiklik olmamıştır, ancak kontrol grubuna kıyasla 96 saat sonra azalma görülmüştür (p0,05). Dimethoate ve malathion kombinasyonunun toksik yanıtı konsantrasyonlarına bağlı olarak değişebildiği tespit edilmiştir. Sonuç olarak, dimethoate ve malathion karışımlarının, D. polymorpha'da AChE ve CYP1A1 aktivitelerini inhibe ettiği ve bu enzimlerin etkili bir biyobelirteç olarak kullanılabileceği ortaya konmuştur.
AtıfOpenAlex1İncele - MakaleAçık Erişim2022Pülümür Nehri (Tunceli)’ndeki Salmo trutta macrostigma (Dumeril, 1858), Cyprinion macrostomum Heckel, 1843 ve Oxynoemacheilus tigris (Heckel, 1843)’in Boy-Ağırlık İlişkileri
Serdar, Osman; Ozcan, Ebru Ifakat
Bu çalışmada; 2017 ve 2019 tarihleri arasında Pülümür Nehri'ndeki Salmo trutta macrostigma (Dumeril, 1858), Cyprinion macrostomum Heckel, 1843 ve Oxynoemacheilus tigris (Heckel, 1843)’in boy-ağırlık ilişkileri araştırılmıştır. Total boy ve ağırlıklar S. t. macrostigma için 14,4-36,3 cm ve 45,2-602 g, C. macrostomum için 7,8-14,0 cm ve 4,9-30,0 g, O. tigris için 4,3-8,6 cm ve 0,7-5,1 g arasında değişim göstermiştir. İncelenen türlerin boy-ağırlık ilişkisi; S. t. macrostigma için W = 0,0093TL3,0655 (R² = 0,9390), C. macrostomum için W = 0,0073TL3,1613 (R² = 0,9008) ve O. tigris için W = 0,0068TL3,1053 (R² = 0,9729) olarak bulunmuştur. S. t. macrostigma ve O. tigris’in b değerlerinin istatistiki olarak 3’ten farklı olmadığı tespit edilmiş (t-testi, p>0,05) ve Pülümür Nehri’ndeki bu 2 türün büyümesinin izometrik olduğu belirlenmiştir. C. macrostomum’un b değerinin istatistiki olarak 3’ten farklı olduğu tespit edilmiş (t-testi, p
AtıfOpenAlex2İncele - MakaleAçık Erişim2022Pülümür Nehri (Tunceli)’ndeki Noktalı İnci Balığı Alburnoides bipunctatus (Bloch, 1782)’un Boy-Ağırlık İlişkileri ve Kondisyon Faktörü
Serdar, Osman; Ozcan, Ebru Ifakat
Bu çalışmada; 2017 ve 2019 tarihleri arasında Pülümür Nehri'nden yakalanan noktalı inci balığı Alburnoides bipunctatus (Bloch, 1782)’un boy-ağırlık ilişkisi ve kondisyon faktörü araştırılmıştır. Örneklerin total boy uzunlukları ve ağırlıkları dişilerde 7,1-11,6 cm ve 2,9-12,3 g; erkeklerde 7,0-11,9 cm ve 2,7-13,5 g arasında değişmiştir. Dişi/erkek oranı 1/0,87 olarak hesaplanmıştır. Boy-ağırlık ilişkileri dişilerde W=0,0111TL2,90 (R2=0,80); erkeklerde W=0,0155TL2,76 (R2=0,83); tüm bireylerde W=0,0136TL2,81 (R2=0,82) olarak belirlenmiştir. Pülümür Nehri’ndeki A. bipunctatus’un negatif allometrik bir büyüme gösterdiği tespit edilmiştir. Boy-ağırlık ilişkisinin dişi, erkek ve tüm bireyler için kuvvetli ve anlamlı olduğu gözlenmiştir (R2=0,82, p<0,001). Kondisyon faktörü değerinin dişilerde 0,670-1,175, erkeklerde 0,632- 1,313 ve tüm bireylerde ise 0,632-1,313 arasında dağılım gösterdiği saptanmıştır.
- MakaleAçık Erişim2022Scopus SJRQ2The Bioremediation Capacity of Sphingomonas melonis for Methomyl-Contaminated Soil Media: RSM Optimization and Biochemical Assessment by Dreissena polymorpha
Önder Erguven, Gokhan; Serdar, Osman; Tanyol, Mehtap; Cikcikoglu Yildirim, Nuran; Yildirim, Numan; Durmus, Barbaros
The bioremediation capacity of S. melonis for methomyl investigated with RSM. Oxidative stress and neurotoxic response determined in D. polymorpha. COD and TOC levels were measured. GSH, MDA levels and CAT, SOD, AChE activities in D. polymorpha exposed to before (group A) and after (group B) bioremediated media during 24 and 96 h were tested by using ELISA kit. AchE activity was decreased 24th h but increased during 96th h in group B compared with group A (p<0.05). MDA levels and CAT activities were decreased during 24 h and 96 h in group B compared with group A (p<0.05). GSH levels were increased during 24 h and 96 h in group B compared with group A (p<0.05). SOD activity did not show a significant change in group B (p<0.05). Methomyl could be effectively remediated by S. melonis and CAT, AChE activities and GSH, TBARS levels of D. polymorpha are useful biomarkers for evaluating the bioremediation capacity.
AtıfScopus5WoS6OpenAlex6İncele - MakaleYalnız künye2022Scopus SJRQ2The use of Gammarus pulex as a model organism for ecotoxicological assessment of ibuprofen and propranolol at environmental relevant concentrations
Cikcikoglu Yildirim, Nuran; Serdar, Osman; Basaran, Senay
The aim of this study is to assess the toxicity of ibuprofen (IBU) and propranolol (PRO) drugs usingGammarus pulex as a model organism. Firstly, the 96 h LC50 values of IBU and PRO were determined and then three sublethal concentrations of the drugs were exposed to G. pulex. The activities of superoxide dismutase (SOD), catalase (CAT) and acetylcholinesterase (AChE) were evaluated. SOD activity decreased in G. pulex exposed to IBU and PRO compared to control. In all groups exposed to IBU, CAT activity increased at different concentrations at 24 and 96 h. In the groups exposed to different PRO concentrations, CAT activities increased after 24 h compared to the control group (p < 0.05). AChE activities increased in all application groups exposed to IBU for 96 hours (p < 0.05). In conclusion, exposure to IBU and PRO resulted in increased oxidative damage. PRO has been found to cause neurotoxicity.
AtıfScopus7WoS5OpenAlex7İncele - MakaleAçık Erişim2021Cyfluthrin Pestisitinin Zebra Midyesi (Dreissena polymorpha) Üzerindeki Etkisinin Bazı Antioksidan Enzim Aktiviteleriyle Belirlenmesi
Serdar, Osman
Yapılan bu çalışmada, Dreissena polymorpha’da ticari insektisit olan Cyfluthrin (CFT)’nin bazı biyokimyasal yanıtları araştırılmıştır. CFT’nin D. polymorpha üzerindeki 96 saatlik LC50 değeri 553,22 ± 27,3 µg/L olarak hesaplanmıştır. D. polymorpha, CFT’nin subletal konsantrasyonlar (LC50 değerinin 1/20, 1/10 ve 1/5)’ına 24 ve 96 saat süre ile maruz bırakılmıştır. D. polymorpha bireylerinde superoksit dismutaz (SOD), katalaz (CAT) ve Glutatyon peroksidaz (GPx) enzim aktiviteleri belirlenmiştir. CFT’ye maruz bırakılan D. polymorpha’da, SOD aktivitesi kontrole kıyasla artmıştır. CAT ve GPx aktivitelerinin kontrole kıyasla inhibe olduğu belirlenmiştir. Sonuç olarak, CFT'ye maruz kalan D. polymorpha bireylerinin oksidatif hasarında artış olduğu belirlenmiştir
AtıfOpenAlex11TR Dizin6İncele - MakaleAçık Erişim2021Farklı Sıcaklık ve Farklı Konsantrasyonlarda Kadmiyuma Maruz Bırakılan Gammarus pulex'te Bazı Biyokimyasal Parametrelerin Değişikliklerinin Belirlenmesi
Serdar, Osman; Çalta, Metin; Aydin, Rahmi
Bu çalışmada Gammarus pulex üzerinde kadmiyum (Cd) toksisitesinin su sıcaklığına bağlı olarak oksidatif stres etkileri araştırılmıştır. Test organizması bireyleri, her sıcaklık (10, 14, 18 °C) için belirli oranlarda (C1; 1/10, C2; 1/20 ve C3; 1/40) LC50 değerlerinin 96 saat boyunca subletal konsantrasyonlara maruz bırakıldı. Cd'ye maruz kalan G. pulex'te belirlenen sıcaklıklarda malondialdehid (MDA) seviyesi, glutatyon peroksidaz (GPx) ve katalaz (CAT) enzim aktiviteleri araştırıldı. Sıcaklık arttıkça MDA seviyesi ve CAT enzim aktivitesi artarken GPx enzim aktiviteleri azaldı. Bu çalışmanın sonuçlarına göre, G. pulex üzerinde Cd'nin neden olduğu biyokimyasal yanıtın sıcaklık ile istatistiksel olarak anlamlı farklılıklara (p<0,05) sahip olduğu belirlenmiştir. Bu çalışmada, risk değerlendirmesi için biyobelirteç olarak MDA seviyelerinin CAT ve GPx ile ilişkili enzimler, Cd maruziyeti, toksisite ve sıcaklık değişimi ile kullanılması yararlı olabilir
AtıfOpenAlex3İncele - MakaleAçık Erişim2021Gadolinyumun Tatlı Su Omurgasızı Dreissena polymorpha Üzerindeki Biyokimyasal Etkileri
Serdar, Osman; Yildirim, Nuran Cikcikoglu; Yildirim, Numan; Tatar, Sule
Bu çalışma, metabolik ve oksidatif stres biyobelirteçlerinin analizi yoluyla, su kirliliğinin iyi bir biyoindikatörü olarak kabul edilen tatlı su omurgasızı Dreissena polymorpha üzerindeki Gadolinyum (Gd) toksisitesini değerlendirmeyi amaçlamaktadır. Öncelikle çalışmamızda Gd’nin D. polymorpha üzerindeki LC50 değeri belirlenmiştir. D. polymorpha, kontrollü koşullar altında 24 ve 96 saatlik bir süre boyunca üç letal olmayan Gd konsantrasyonuna (1/20, 1/10 ve 1/5 LC50 değerine) maruz bırakılmıştır. TBARS ve CYP1A1 seviyeleri ELISA kiti kullanılarak belirlenmiştir. CYP1A1 ve TBARS düzeylerinin tüm gruplarda 24. ve 96. saatte kontrol grubuna göre arttığı görülmüştür. Bu sonuçlar, Gd'nin tatlı su omurgasızları, D. polymorpha için bir risk olarak kabul edilebileceğini ve bu nedenle alıcı ortamlara deşarjının denetlenmesi gerektiğini göstermektedir.
AtıfOpenAlex8TR Dizin4İncele - MakaleAçık Erişim2021Pülümür Nehri (Tunceli)’ndeki Alburnus mossulensis (Mossul bleak) Heckel, 1843 ‘ün Boy-Ağırlık İlişkisi ve Kondisyon Faktörü
Serdar, Osman; Ozcan, Ebru Ifakat
Bu çalışmada; 2017 ve 2019 tarihleri arasında Pülümür Nehri'nden yakalanan Alburnus mossulensis’in Heckel, 1843 cinsiyet dağılımı, boy-ağırlık ilişkisi ve kondisyon faktörü araştırılmıştır. İncelenen bireylerin % 58,80’inin (304 adet) dişi, % 41,20’sinin (213 adet) erkek olduğu ve dişi/erkek oranının 1:0,70 olduğu belirlenmiştir. Örneklenen balık total boyları ve ağırlıkları dişilerde 6,7-22,7 cm ve 3,5-74,3 g, erkeklerde ise 7,0-22,1 cm ve 3,6-70,2 g arasında dağılım göstermiştir. Boy-ağırlık ilişkisi dişilerde W = 0,0105L2,86 (R² = 0,92), erkeklerde W = 0,0160L2,70 (R² = 0,93) ve tüm bireylerde W= 0,0126L2,79 (R² = 0,92) olarak bulunmuştur. Büyümenin negatif allometrik olduğu tespit edilmiştir. Dişi, erkek ve tüm bireylerde total boy ile ağırlık arasında çok kuvvetli bir ilişki bulunduğu görülmüştür (R² > 0,92). Kondisyon faktörü değerleri dişilerde 0,456-1,237, erkeklerde 0,474-1,304 ve tüm bireylerde ise 0,456-1,304 arasında değişmektedir.
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