Sürdürülebilir Kalkınma Amaçları
Yayınlar
- MakaleYalnız künye2024Scopus SJRQ3The RSM-CCD Optimization of Fenton Process for Industrial Wastewater Treatment and Determination of Treatment Efficiency Using Some Biochemical Responses in Dreissena polymorpha
Tanyol, Mehtap; Serdar, Osman; Ketenalp, Zozan; Yildirim, Nuran Cikcikoglu; Erguven, Gokhan Onder; Yildirim, Numan
The study investigated antioxidant biomarkers in Dreissena polymorpha to evaluate the efficiency of Fenton process (FP) in Organized Industrial Zone Wastewater (OIZW) treatment. Antioxidant enzymatic activities, including superoxide dismutase (SOD) and catalase (CAT), glutathione peroxidase (GPX), non-enzymatic antioxidants (GSH) and the indicator of lipid peroxidation (TBARS) were measured in D. polymorpha in response to treated OIZW by FP. The performance of the FP has been optimized with response surface methodology (RSM). The optimum conditions (reaction time, and H2O2 and Fe2+ concentration) for OIZW by the FP process were determined. The results showed that the highest COD removal efficiency (83.94 %) was obtained when as Fe2+ concentration=402.35 mg/L, H2O2 concentration=4100.30 mg/L, and reaction time=10.10 min (desirability=1.000). SOD and CAT enzyme was decreased in the 1/25 diluted OIZW group compared to control group. CAT and SOD enzyme activities were increased after treatment compared to control groups. No statistically significant changes were observed in GSH levels and GPx activities. TBARS levels were increased 1/25 diluted OIZW exposure groups compared to the control group. TBARS levels were decreased after treatment. The results revealed that antioxidant parameters are useful biomarkers for determining the treatment efficiency of the FP.
AtıfScopus2WoS1OpenAlex2
İncele - MakaleAçık Erişim2023Scopus SJRQ3Bioremediation of herbicide diquat dibromide from aqueous solution with white rot fungus Phanerochaete chrysosporium
Yildirim, Numan; Ciftci, Emine; Erguven, Gokhan Onder; Durmus, Barbaros
In this study, the fungal bioremediation of herbicide diquat dibromide (DD) by white rot fungus Phanerochaete chrysosporium was studied. After bioremediation, the reduction in toxicity of the remediated media was evaluated by Daphnia magna as a model organism. For bioremediation studies, flask-level batch experiments were performed at different DD concentrations (10, 20 and 50 ppm) under static and agitated conditions. The highest reduction rates of chemical oxygen demand and total organic carbon were determined as 85.92 (for 20 ppm DD) and 87.46% (for 10 ppm DD), respectively under agitated conditions. After bioremediation, D. magna bioassay was used for toxicity assessment during 24, 48 and 72-h periods. In current study, it was observed that P. chrysosporium has very effective bioremediation capacity for diquat dibromide. The D. magna bioassay revealed that P. chrysosporium provides effective reduction in toxicity of diquat dibromide.
- MakaleAçık Erişim2023Scopus SJRQ4Single and combined effects of dimethoate and malathion on oxidative stress biomarkers in the non-target freshwater mussel Dreissena polymorpha
Serdar, Osman; Yildirim, Numan; Yildirim, Nuran Cikcikoglu
This study evaluates the single and combined effects of dimethoate (DI) and malathion (MA) on oxidative stress biomarkers in the freshwater mussel Dreissena polymorpha, estimating the potential harm of these pesticides on aquatic ecosystems. Superoxide dismutase (SOD) and catalase (CAT) activities, and glutathione (GSH) and lipid peroxidation (TBARS) levels were determined in D. polymorpha exposed to sublethal concentrations of DI, MA and a combination of the two during 24 and 96 h periods. The results showed that the GSH levels were decreased but the TBARS levels were increased in all the exposure groups after 24 and 96 h compared to the control. It was observed that SOD activity decreased but CAT activity increased in all the DI exposure groups after 24 h compared to the control. At the end of 96 hours, it was observed that CAT and SOD activities increased again in some exposure groups compared to the control. Exposure time also had an effect on biomarkers in different levels. According to the results, the cytotoxicity of DI and MA combination depended on their concentrations. DI or/ and MA, in sub-lethal concentrations, induced oxidative damages in D. polymorpha. Combined exposure of the pesticides can alter their toxicity and may be evidence of increased toxicity and oxidative stress.
AtıfScopus3WoS3OpenAlex2İncele - MakaleAçık Erişim2023Scopus SJRQ3The biosorption of Co(II) by Homalothecium sericeum from aqueous solutions under batch conditions: response surface methodology optimization and Daphnia magna bioassay
Tanyol, Mehtap; Korkmaz, Volkan; Yildirim, Numan; Erguven, Gokhan Onder; Ayhan, Nagihan Karaaslan; Alatas, Mevlut
In the present work, Homalothecium sericeum biomass was used for removal of Co(II) from aqueous solution. The effects of process parameters (contact time, biosorbent amount, and initial Co(II) con-centration) on the response (Co(II) % removal) were investigated using a batch adsorption system optimized with response surface methodology/central composite design method. Under optimized conditions the maximum Co(II) removal efficiency achieved is 94.07% at an initial Co(II) concentration of 2.05 mg/L, a H. sericeum amount of 0.34 g/50 mL, and a process time of 36.09 min. In this study, the toxicity of the medium after biosorption carried out under optimized conditions was also tested with Daphnia magna bioassay. When mortality results with D. magna were evaluated, although a removal efficiency of 94.07% was obtained after biosorption, this was not sufficient to reduce mortality of D. magna.
AtıfScopus1WoS2İncele - MakaleAçık Erişim2022Dimethoate ve Malathion Pestisitlerinin Dreissena polymorpha (Pallas, 1771) Üzerindeki Kombine Etkisinin Sitokrom P450 ve Asetilkolinesteraz Kullanılarak Belirlenmesi
Serdar, Osman; Yildirim, Nuran Cikcikoglu; Yildirim, Numan
Bu çalışmada, Dreissena polymorpha’da organofosforlu dimethoate ve malathion etken maddeli insektisit karışımlarının toksisitesini ortaya çıkarmak için sitokrom P450 (CYP1A1) ve Asetilkolinesteraz (AchE) aktiviteleri ölçülmüştür. Dimethoate ve malathion'un öldürücü konsantrasyonu (LC50) 96 saat maruziyet sonunda 40,82±2,54 mg/L olarak hesaplanmıştır. D. polymorpha dimethoate ve malathionun üç subletal konsantrasyonuna (LC50 değerine 1/16, 1/8 ve 1/4 oranla) 24 saat ve 96 saat boyunca maruz bırakılmıştır. CYP1A1 ve AChE seviyeleri, ticari kit kullanılarak mikroplaka okuyucuda ölçülmüştür. AChE aktivitelerinin kontrol grubuna göre 96 saat sonra tüm maruziyet gruplarında düştüğü gözlenmiştir. Maruziyet süreleri karşılaştırıldığında 96. saat sonunda enzim aktivitelerinde istatistiksel olarak anlamlı bir değişiklik olmadığı belirlenmiştir. Tüm gruplarda 24 saatin sonunda CYP1A1 seviyelerinde istatistiksel olarak anlamlı bir değişiklik olmamıştır, ancak kontrol grubuna kıyasla 96 saat sonra azalma görülmüştür (p0,05). Dimethoate ve malathion kombinasyonunun toksik yanıtı konsantrasyonlarına bağlı olarak değişebildiği tespit edilmiştir. Sonuç olarak, dimethoate ve malathion karışımlarının, D. polymorpha'da AChE ve CYP1A1 aktivitelerini inhibe ettiği ve bu enzimlerin etkili bir biyobelirteç olarak kullanılabileceği ortaya konmuştur.
AtıfOpenAlex1
İncele - MakaleYalnız künye2022Bioremediation Performance of Sphingomonas melonis and Bacillus\rmuralis on Herbicide Diquot Dipremide-(ethylene-d4)
Erguven, Gokhan Onder; Yildirim, Numan; Koçak, Emel; Durmus, Mehmet Harun Bogacbarbaros; Aydin, Ekrem
Bioremediation is a process that utilizes the degradation potential of microorganism to provide a cost-effective and reliable\rapproach for pesticide biodegradation. For this purpose, chosen bacteria Sphingomonas melonis and Bacillus muralis were isolated\rfrom an agricultural soil sample. The biodegradation performance of these isolated bacteria at different Diquot Dipremide-\r(ethylene-d4) (DDE4) herbicide concentrations (250, 500 and 1000 ppm) was investigated under submerged culture conditions.\rBiodegradation performance of isolated bacteria was monitored with COD, TOC and, BOD5 reduction rates in culture medium at\rdifferent incubation periods. According to the results; S. melonis has the highest bioremediation capacity for COD removal (91%\rat 250 ppm). For TOC, B. muralis has the highest removal rate as 82% at 250 ppm. On the other hand, For BOD5 at 250 ppm 85%\rS. melonis showed the best removal performance. Most effective removal rate at 250 ppm concentrations was obtained as 91%\rand 88 by S. melonis and B. muralis respectively at the end of the 216th hour for COD. Additionally, the increase in turbidity\rrelated with population dynamics at the end of the 216 th hour positively effected the bioremediation parameters included COD,\rTOC and BOD5 reductions. These results showed that it can be used for effective COD, TOC and BOD5 removal in S. melonis and\rB. muralis on DDE4 remediation
- MakaleAçık Erişim2022Scopus SJRQ3Decolorization of Remazol Brilliant Violet 5R and Brilliant Green dyes by Hypnum cupressiforme using response surface methodology-central composite design methodology: toxicity assessment studies by Daphnia magna
Korkmaz, Volkan; Erguven, Gokhan Onder; Tanyol, Mehtap; Alatas, Mevlut; Yildirim, Numan
This study is the first report that aims to optimize the removal of Remazol Brilliant Violet 5R (RBV 5R) and Brilliant Green (BG) from synthetic solutions using Hypnum cupressiforme samples from Abant Mountains of Bolu (Turkey) as biosorbent and to conduct toxicity evaluation studies of treated wastewater using Daphnia magna. Optimization was performed using central composite design under response surface methodology with the operational factors of dye concentration, H. cupressiforme dose, and process time. The optimal conditions for dye concentration, H. cupres-siforme dose, and process time obtained by the numerical analysis were 19.02 mg/L, 0.18 g/50 mL and 110.59 min for RBV 5R, while these values were 18.57 mg/L, 0.18 g/50 mL and 99.45 min for BG, respectively. At the optimum values, maximum RBV 5R and BG removal were achieved as 99.39% and 99.22%, respectively. The D. magna mortality rates showed that treatment with adsorption were sufficient to remove the complete toxicity of dyes especially for RBV 5R.
AtıfScopus2WoS2OpenAlex2İncele - MakaleAçık Erişim2022Scopus SJRQ2The Bioremediation Capacity of Sphingomonas melonis for Methomyl-Contaminated Soil Media: RSM Optimization and Biochemical Assessment by Dreissena polymorpha
Önder Erguven, Gokhan; Serdar, Osman; Tanyol, Mehtap; Cikcikoglu Yildirim, Nuran; Yildirim, Numan; Durmus, Barbaros
The bioremediation capacity of S. melonis for methomyl investigated with RSM. Oxidative stress and neurotoxic response determined in D. polymorpha. COD and TOC levels were measured. GSH, MDA levels and CAT, SOD, AChE activities in D. polymorpha exposed to before (group A) and after (group B) bioremediated media during 24 and 96 h were tested by using ELISA kit. AchE activity was decreased 24th h but increased during 96th h in group B compared with group A (p<0.05). MDA levels and CAT activities were decreased during 24 h and 96 h in group B compared with group A (p<0.05). GSH levels were increased during 24 h and 96 h in group B compared with group A (p<0.05). SOD activity did not show a significant change in group B (p<0.05). Methomyl could be effectively remediated by S. melonis and CAT, AChE activities and GSH, TBARS levels of D. polymorpha are useful biomarkers for evaluating the bioremediation capacity.
AtıfScopus5WoS6OpenAlex6İncele - MakaleAçık Erişim2022Scopus SJRQ3The use of Phanerochaete chrysosporium as an alternative bioremedia-tion tool of heavy metal, chemical oxygen demand and phosphate from landfill leachate
Yildirim, Numan; Kardas, Elif; Ince, Muharrem
In this study, in order to investigate the bioremediation efficiency of Phanerochaete chrysosporium, chemical oxygen demand (COD) reduction, total phosphate levels and heavy metal (Fe2+, Mn2+, Zn2+ and Cu2+) removal were determined in five different experimental groups (control, X, Y, Z, and T) under static culture conditions containing leachate at different incubation periods (5, 10, and 20 d). At the end of the 20th day of incubation, 84% Mn2+ and Zn2+ removal was achieved in X application group. In the same application group, 90.88% COD reduction was also obtained. The lowest total phosphate value was determined as 2.63 g L–1 in T group compared with control group (5.0 g L–1). Similar improvements have been identified in other application groups in terms of metal removal, total phosphate levels and COD reduction. As a result, the findings obtained from our study show that white rot fungus P. chrysosporium can be used as an alternative biological resource in the studies for improving landfill leachate in terms of metal removal efficacy, COD and total phosphate level reduction capacity.
AtıfScopus3WoS1OpenAlex3İncele - MakaleAçık Erişim2021Removal of Chemical Oxygen Demand, Total Organic Carbon and Color from Textile Industry Wastewater by Using Phanerochaete chrysosporium (ME446)
Erguven, Gokhan Onder; Yildirim, Numan; Çelik, Aytekin
Wastewater discharge from textile industries concern environmental risks. Superiority of microbial methods over other high cost combined methods includes conversion of persistent organic materials to non-toxic last materials, sustainability, low cost, and comfortable. Textile wastewaters can have opposite effects on the quality of water in total organic carbon (TOC) and Chemical oxygen demand (COD). Agitation cultures can reach the surface area of the dyes, whereas a static cannot do. Biotreatment of textile wastewater from the dyeing process using white-rot fungus Phanerochaete chrysosporium (P.C) was investigated in agitated and static culture conditions. Dye is the major pollutant component in this wastewater includes some different organic pollutants. The treatment mediums containing distilled water in 1:10 ratio of wastewater were compared for treatment efficiency of P.C. Especially in agitated conditions at 27 oC and 150 rpm, it was achieved a successful treatment results. Under these conditions, a 48h long treatment reduced by 91,46 % of the original COD (from 1484 mg l-1) and by 94,92% the TOC (initial was 723.66 mg l-1). Moreover, treatment reduced color by 86,28 % from 3.550 A540 to 0.487 A540 at the end of the study. The decolorization properties of P.C obtained high performance and we determined P.C showed up to effective removal rate for COD and TOC within 48 hours. We suggest that these fungus pellets of P.C can reach the decolorization and can be a useful tool for bioremediation of textile dye wastewater within a short time period.
AtıfOpenAlex3İncele - MakaleAçık Erişim2021The Effect of Gadolinium and Lanthanum on the Mortality ofDaphnia magna
Korkmaz, Volkan; Ergüven, Gökhan Önder; Yildirim, Numan; Cıkcıoğlu Yıldırım, Nuran
Rare earth elements (REEs) have been recently identified as emergent contaminants because of their numerous and increasingapplications in technology. The impact of REEs on downstream ecosystems, notably aquatic organisms, is of particular concern, but has to date been largely overlooked. The purpose of this study were to generate toxicological information regarding thesepoorly studied Gadolinium and Lanthanum elements to determine the current risk associated with these elements. The results of this work indicate notable D. magna mortality in high concentration of La and Gd. The highest mortality rate was determined as100% at 100 mg/L concentration for both La and Gd at the end of the 24hour . At the end of the 48 hours, the highest mortalityrate for both La and Gd was observed at 100 mg/L concentration. No mortality was determined at 48 hours at a concentration of10 mg/l Gd. 100% mortality was observed for two elements at 50 and 100 mg/L concentrations at 72 hours. in the natural living water no death was seen in this time period. The findings from our study also indicate that Gd is more toxic than La. Due to thismortality effect of La and Gd to D. magna, it will be important for the ensuring continuity of the ecosystem to monitor especiallyaquatic environments and to treat them with appropriate treatment methods from contaminated environments.
AtıfOpenAlex2İncele - MakaleAçık Erişim2021Gadolinyumun Tatlı Su Omurgasızı Dreissena polymorpha Üzerindeki Biyokimyasal Etkileri
Serdar, Osman; Yildirim, Nuran Cikcikoglu; Yildirim, Numan; Tatar, Sule
Bu çalışma, metabolik ve oksidatif stres biyobelirteçlerinin analizi yoluyla, su kirliliğinin iyi bir biyoindikatörü olarak kabul edilen tatlı su omurgasızı Dreissena polymorpha üzerindeki Gadolinyum (Gd) toksisitesini değerlendirmeyi amaçlamaktadır. Öncelikle çalışmamızda Gd’nin D. polymorpha üzerindeki LC50 değeri belirlenmiştir. D. polymorpha, kontrollü koşullar altında 24 ve 96 saatlik bir süre boyunca üç letal olmayan Gd konsantrasyonuna (1/20, 1/10 ve 1/5 LC50 değerine) maruz bırakılmıştır. TBARS ve CYP1A1 seviyeleri ELISA kiti kullanılarak belirlenmiştir. CYP1A1 ve TBARS düzeylerinin tüm gruplarda 24. ve 96. saatte kontrol grubuna göre arttığı görülmüştür. Bu sonuçlar, Gd'nin tatlı su omurgasızları, D. polymorpha için bir risk olarak kabul edilebileceğini ve bu nedenle alıcı ortamlara deşarjının denetlenmesi gerektiğini göstermektedir.
AtıfOpenAlex8TR Dizin4İncele - MakaleYalnız künye2021Scopus SJRQ2The assessment of basic blue 41 textile dye decolorisation using the Fenton process: response surface methodology optimisation and toxicity evaluation
Tanyol, Mehtap; Cikcikoglu Yildirim, Nuran; Tatar, Sule; Serdar, Osman; Yildirim, Numan
The biochemical biomarkers (Catalase (CAT), glutathione s-transferase (GST), cytochrome P4501A1 (CYP1A1450) activities and glutathione (GSH), malondialdehyde (MDA) levels) of D. polymorpha exposed to the basic blue 41 (BB41) solutions for 24 and 96 h before and after the Fenton process (FTP) were evaluated to investigate the efficiency of the FTP. The optimum conditions for the removal of BB41 were set using central composite design (CCD), which is a response surface methodology (RSM) module in the Design-Expert® software. The optimal conditions were found to be 27.87 min, 3.49 mg/L, and 0.04 for reaction time, initial BB41 concentration, and Fe2+/H2O2 molar ratio, respectively, resulting in maximum (99%) removal efficiency. After FTP, the MDA and GSH levels decreased significantly during the 24 and 96 h. CAT activities were increased after treatment. No statistically significant changes were observed in the CYP1A1 levels after FTP. GST activities decreased during 24 h but increased during 96 h after FTP. CYP1A1450, catalase, GST activities and MDA, GSH levels of D. polymorpha are useful biomarkers to evaluate the efficiency of FTP. This study suggests that BB41 dye must be further treated after the FTP to minimise the ecotoxicological risk.
AtıfScopus8WoS6OpenAlex8İncele - MakaleAçık Erişim2021Scopus SJRQ1The bioremediation of glyphosate in soil media by some newly isolated bacteria: The COD, TOC removal efficiency and mortality assessment for Daphnia magna
Korkmaz, Volkan; Yildirim, Numan; Erguven, Gokhan Onder; Durmus, Barbaros; Nuhoglu, Yasar
In this study, the bioremediation capacity of three isolates of Bacillus aryabhattai, Pseudomonas azotoformans and Sphingomonas pseudosanguinis and their consortia at glyphosate added soil medium investigated via the reduction of chemical oxygen demand (COD) and total organic carbon (TOC) parameters in filtrate waters obtained from a designed bioremediation setup. Additionally, mortality assays were performed on the filtrate waters with model organism Daphnia magna. According to the results of the experiments, at the end of the 11 days, the highest COD reduction rate seen in media with S. pseudosanguinis as 92.1% while TOC rate was 69.13% in consortia media. While the mortality rate was 10, 10 and 5% on B, P and S application groups at the end of the 72th respectively, these rates were 100% in control group at the end of the 48th. According to the results obtained from bioremediation and mortality assessment, these bacteria can reach the high bioremediation rate one by one and in consortia media.
AtıfScopus14OpenAlex16İncele - MakaleAçık Erişim2021Scopus SJRQ4DETERMINATION OF THE ANTIOXIDANT PARAMETERS OF COMMONLY AVAILABLE BLACK PINE (PINUS NIGRA J.F. ARNOLD)
Yildirim, Nuran Cikcikoglu; Gezer, Esref; Yildirim, Numan
The antioxidant parameters of black pine (Pinus nigra J.F. Arnold) needles samples collected from different areas in the province of Diyarbakir, Turkey were studied. Black pine needles samples were collected from three different locations (Egil, Kosuyolu, and Diyarbakir industrial area) in the province of Diyarbakir in summer and winter seasons. The activity of enzymatic antioxidants such as superoxide dismutase (SOD), catalase (CAT), glutathione peroxidase (GPX), glutathione S-transferase (GST) and malondialdehyde (MDA) levels of the samples were determined by ELISA method. Results showed that SOD enzyme activities of the samples collected in summer from Kosuyolu and the industrial area decreased compared to the control group, but increased in the samples collected in winter from the same areas. When compared to the control group, it was observed that the MDA levels of the samples collected from Kosuyolu and the industrial area increased.
- MakaleYalnız künye2019Antioxidant Enzyme Responses to Lead Exposure of Gammarus pulex
Serdar, Osman; Yildirim, Numan; Tatar, Sule; Cıkcıkoğlu Yıldırım, Nuran
It is aimed to investigate the activity response of the antioxidant enzymes superoxide dismutase, catalase and glutathione peroxidase of G. pulex exposed to lead. The G. pulex was exposed synthetic solutions containing 10, 30, 50 µg/L lead for 96 h. Superoxide dismutase, catalase, glutathione peroxidase activities were determined by using ELISA kit. The differences in superoxide dismutase activities were statistically insignificant (P>0.05). Catalase decreased in all treatment groups after lead exposure (P<0.05). The glutathione peroxidase activities were decreased depending on lead exposure (P>0.05). Our findings suggest that lead can induce oxidative stress by generating reactive oxygen species and inhibiting sulfhydryl dependent antioxidant enzymes activities. In conclusion, alterations in antioxidant enzymes may potentially be used as sensitive biomarkers for risk assessment of lead in the environment and may contribute to the establishment of discharge regulations.
AtıfTR Dizin1İncele - MakaleAçık Erişim2019Congo Red Effects on Detoxification and Metabolic Enzymes in Rainbow Trout (Oncorhynchus mykiss Walbaum, 1792)
Danabaş, Durali; Yildirim, Nuran Cikcikoglu; Yildirim, Numan; Aksu, Önder
The aim of this study was to evaluate the activities of the biotransformation enzymes (Glutatyon STransferase (GST) and cytochrome P450 (CYP1A1)) and metabolic enzyme (Lactatedehydrogenase-LDH) in the liver of rainbow trout (Oncorhynchus mykiss) after 24 and 48 hourexposure to 0.5, 1.0 and, 2.0 mg l-1 of Congo red (CR). Enzyme activities were determined by usingcommercial kits with ELISA method. Congo red altered the activities of GST, CYP1A, and LDHenzymes in liver tissue of O. mykiss in a dose-dependent manner. The statistical differences in GSTactivities among the groups for 24 and 48 h were significant, but, LDH activities were significantfor only 24 h. Exposure duration of CR didn’t affect the biochemical response of rainbow trout.Thus, CR exposure changed the biotransformation and metabolic enzymes, and the changes of theseenzymes activities may be used as a potential bioindicator of the CR exposure.
- MakaleYalnız künye2019Scopus SJRQ2Gammarus pulex as a Model Organism to Assess the Residual Toxicity of Slaughterhouse Wastewater Treated by Electrocoagulation Process
Yildirim, Nuran Cikcikoglu; Tanyol, Mehtap; Serdar, Osman; Yildirim, Numan
It was assessed the efficiency of the electrocoagulation (EC) in slaughterhouse wastewater (SW) treatment by using antioxidant parameters of Gammarus pulex. The SW was treated by EC. Superoxide dismutase (SOD), glutathione peroxidase (GPx) and catalase (CAT) activities and malondialdehyde (MDA) levels in G. pulex exposed to pre- and post-treated of the SW during 24 h and 96 h were analysed. Standard methods were applied during the analysing process of the physicochemical quality parameters for both untreated and treated SW. All measured physicochemical parameters were decreased following the treatment process via EC. After the treatment process, it was observed that while SOD activities and MDA levels were decreased, CAT activities were increased and GPx activities did not exhibit any change. In conclusion, the present study demonstrated the abilities of untreated SW to promote oxidative stress in model organism. The SOD, CAT activities and MDA levels in G. pulex revealed that EC process were efficient in the SW treatment.
AtıfScopus10WoS9OpenAlex13
İncele - MakaleYalnız künye2019Scopus SJRQ2Phanerochaete chrysosporium as a model organism to assess the toxicity of municipal landfill leachate from Elazığ, Turkey
Yildirim, Numan; Yildirim, Nuran Cikcikoglu; Tatar, Sule; Alp, Hevidar
In order to evaluate the potential ecological risk and the toxic effect of landfill leachate (LL), Phanerochaete chrysosporium was exposed to LL and their biochemical response was observed by using antioxidant parameters. Phanerochaete chrysosporium, ME 446, was kept at 4 °C after being sub-cultured at 28 °C on Sabouraud Dextrose Agar (SDA). Superoxide dismutase (SOD), catalase (CAT) activities, and malaondialdehyde (MDA) and glutathione (GSH) levels of P. chrysosporium exposed to different dilution rates of leachate (1/10 and 1/20) for 24 and 96 h were analyzed by using the ELISA method. The physiochemical parameters such as pH, conductivity, total dissolved solids (TDS), dissolved oxygen (DO), chemical oxygen demand (COD) of leachate, and reference water were analyzed by using the YSI Professional Plus handheld multiparameter meter. In this study, SOD activities were decreased in the application groups compared with the Control Group at the 24th and 96th hours. CAT activities and GSH levels increased in the application groups compared with the Control Group at the 24th hour but decreased at the 96th hours. MDA levels increased in all of the application groups when compared with the Control Group for both 24 and 96 h. Different concentration of LL induces oxidative stress in P. chrysosporium, increased CAT activity and MDA levels, and decreased SOD activity and GSH levels.
AtıfScopus7WoS5OpenAlex6İncele - MakaleYalnız künye2019Scopus SJRQ2The Evaluation of Imidacloprid Remediation in Soil Media by Two Bacterial Strains
Erguven, Gokhan Onder; Yildirim, Numan
Imidacloprid (C9H10ClN5O2) is used as the most recommended type of insecticide in vegetable farming worldwide. Two types of bacteria (Methylobacterium radiotolerans and Microbacterium arthrosphaerae) were isolated from a corn farming field in the Thrace region of Turkey, and then consortia of these bacteria were prepared from equal volumes of 107 CFU/ml for each bacterium type. Imidacloprid remediation studies were carried out during 18 days in soil test units. The water filtered from these soil test units was determined for chemical oxygen demand (COD) and biochemical oxygen demand (BOD5) to determine the optimum concentration of microorganisms to ascertain the best removal efficiency of Imidacloprid. COD removal rates were 98.7%, 96.4% and 51.6% with 80, 40, and 20 ml volumes of the consortia of bacteria, respectively, at the end of 18 days. The BOD5 removal rates were 88.4%, 78.6% and 49.9% in the same volumes of bacteria, respectively. As a result of this study, we have found that this bacterial consortium is very effective for the bioremediation of this insecticide at the two volumes of 40 and 80 ml, both being better than 20 ml.
AtıfScopus35WoS34OpenAlex37İncele