Sürdürülebilir Kalkınma Amaçları

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Yazarlar

• Aksu, Onder(1)
• Altinterim, Basar(1)
• Danabas, Durali(1)
• Gönenci, Ramazan(1)
• Ispir, Unal(1)
• Işgör, Mehmet Mustafa(1)
• Karahan, Murat(1)
• Kucukgul, Altuğ(1)
• Kucukgul, Azime(1)
• Kutlu, Banu(1)
• Küçükgül, Altuğ(1)
• Küçükgül, Azime(1)
• Saglam, Naim(1)
• Sarieyyüpoglu, Mustafa(1)
• Seker, Engin(1)
• Tuba Otay(1)
• Çetinkaya, Burhan(1)
• Özsoy, Şule Yurdagül(1)
• İlon Veysiye Erkil(1)

• MakaleYalnız künye2019Scopus SJRQ2/r/munzur/3527
  Investigation of the anti-apoptotic activity of ozone therapy in rainbow trout macrophages infected with Yersinia ruckeri

  Küçükgül, Azime; Küçükgül, Altuğ; Gönenci, Ramazan; Özsoy, Şule Yurdagül; Kutlu, Banu; Işgör, Mehmet Mustafa

  Yersinia ruckeri is the causal agent of enteric redmouth (ERM) in trout. This study aimed to investigate the effectiveness of nontoxic ozone on Y. ruckeri-infected trout cells in in vitro conditions. Rainbow trout macrophages (RTS-11) were exposed to Y. ruckeri for 24 h after being incubated with the ozonized medium at nontoxic concentration. Effective concentrations of nontoxic ozone (3 gamma) and bacteria (1.5 x 108 cfu/mL) were determined by viability tests. The expression of caspases 1, 3, 8, and 12 was determined by real-time PCR and protein levels investigated by ELISA method. Apoptotic-necrotic cell ratios were determined by acridine orange and ethidium bromide staining. Bacterial stimulations of caspases (1, 3, 8, and 12) were suppressed by ozone mostly at the translational level. While bacterial infection increased the count of apoptotic and necrotic cells at different stages, ozone significantly reduced this condition. Obtained data indicate possible use of nontoxic ozone as a safe and effective treatment alternative for increasing host defenses and eliminating the infection in the case of yersiniosis in fish.

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  AtıfWoS1·OpenAlex3

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• MakaleYayıncıda açık erişim2018Scopus SJRQ3/r/munzur/3543
  The effects of common yarrow (Achillea millefolium Linnaeus), cinnamon (Cinnamomum zeylanicum Blume) and rosemary (Rosemarinus officinalis Linnaeus) hydrosols on the some immunological and hematological parameters of common carp (Cyprinus carpio L., 1758) against to Yersinia ruckeri

  Altinterim, Basar; Danabas, Durali; Aksu, Onder

  In this study, the effects of some plant hydrosols (distilled plant waters) based upon some hematological parameters and Nitroblue Tetrazolium (NBT) activities in the common carp (Cyprinus carpio Linnaeus, 1758) infected with Yersinia ruckeri were investigated. In the trial, it was utilized totally 200 common carps with 54.3±6.7 g mean live weight and 15.7±1.8 cm mean total lenght. The 10% rate of the common yarrow (Achillea millefolium Linnaeus) hydrosol; 0.5% rate of the cinnamon (Cinnamomum zeylanicum Blume) hydrosol; and 5% rate of the rosemary (Rosemarinus officinalis Linnaeus) hydrosol were applied to fish as a bath treatment. The erythrocyte (RBC), leukocyte count (WBC), hematocrit value (HCT), haemoglobin amount (Hg), mean corpuscular volume (MCV), mean corpuscular haemoglobin (MCH), mean corpuscular haemoglobin concentration (MCHC), and activities of NBT in the blood samples taken from the caudal vena of the control and experimental fish groups were analyzed in the 7 th , 14 th , and 21 st days of the exposure treatment. At the end of the research, HCT, Hg, RBC, WBC, MCH and MCV values decreased in the C-2 Group (the control group contain pathogen) compared to the C-1 Group (the control group no contain pathogen), except MCHC value. The NBT activities in the C-1 Groups increased at the 14 th day, but decreased quite a few at the 21 st day. It has been consequently reached the conclusion that the bath treatments of the some plant hydrosols might be beneficial in increasing of antibacterial properties and in strengthening of defense mechanisms of common carp against Y. ruckeri pathogen.

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  AtıfScopus4·WoS4·OpenAlex8

  İncele
• Yüksek Lisans TeziAçık Erişim2017/r/munzur/7239
  Balık patojeni olan Yersinia ruckeri üzerine rezene esansiyel yağlarının etkisinin in vitro araştırılması

  İlon Veysiye Erkil

  Katkısız doğal ürünlerin ticari antibiyotiklere karşı alternatif bir tedavi olabileceği düşüncesiyle yapılan bu çalışmada, rezene (Foeniculum vulgare Miller) esansiyel yağının Enterik Kızıl Ağız Hastalığının etkeni olan Yersinia ruckeri'ye karşı inhibitör etkileri in vitro olarak araştırılmıştır. Rezene esansiyel yağının kimyasal bileşenleri GC ve GC–MS analizi ile belirlenmiş ve ana bileşeninin trans-anetol (% 67,99) olduğu saptanmıştır. Rezene esansiyel yağının antimikrobiyal etkisi olup olmadığını belirlemek üzere ise disk difüzyon metodu kullanılmış, oluşturdukları inhibisyon zonları ölçülmüştür. Bu amaçla rezene esansiyel yağının iki deneysel konsantrasyonu (5 ve 10 µL) uygulanmış, kontrol grubu olarak ise antibiyotik (Streptomisin ve Tetrasiklin) kullanılmıştır. Rezene esansiyel yağı 5 µL/disk uygulaması ile Y. ruckeri'nin inhibisyon zonu 10,52 ± 1,04 mm; 10 µL/disk ile muamale edilen konsantrasyonda ise 13,10±1,67 mm olduğu gözlenmiştir. Streptomisin ve Tetrasiklin ise sırasıyla 21,05±3,23 ve 27,72±2,02 mm zon oluşumuyla güçlü bir antibakteriyel aktiviteye sahip oldukları belirlenmiştir. Denenen iki konsantrasyon kontrol grubu antibiyotiklerle karşılaştırılmış ve daha zayıf bir inhibisyon zonu oluşturdukları anlaşılmıştır. Bu araştırma ile rezene esansiyel yağının Y. ruckeri'ye karşı antibakteriyel etkili olduğu saptanmıştır.

  İncele
• Yüksek Lisans TeziAçık Erişim2017/r/munzur/7238
  Ozon uygulamasının bakteriyel balık patojeni Yersinia ruckeri üzerine etkinliğinin incelenmesi

  Tuba Otay

  Bu çalışmada, Yersinia ruckeri'nin ozon gazına duyarlığı araştırıldı. Y. ruckeri (ATCC 29473) triptik soy agar (TSA) besiyerinde inoküle edilerek 22 oC'de 24-48 saat inkübe edildi. İnkübasyon süresi sonunda elde edilen saf kültürden 100µl'lik fizyolojik tuzlu su (FTS - % 0,9 NaCl - Sigma) içeren tüplerde 0.5 Mcfarland bulanıklığına karşılık gelen (UV spektrofotometre ile OD600nm=0.132 absorbans) 1.5x108 cfu/ml konsantrasyonunda bakteri süspansiyonu hazırlandı. Bu konsantrasyondan 1:10 oranında seri dilüsyonlar yapıldı. 1.5x104 cfu/ml dilüsyonunda bakteri konsantrasyonu, farklı sürelerde (5 dk, 10 dk, 15 dk ve 30 dk) ozona gazına maruz bırakıldı. Bakterinin ozon gazına duyarlılığı 12 ve 40 γ/ml konsantrasyonlarına sahip jeneratör yardımıyla belirlendi. Elde edilen verilere göre, Y. ruckeri'nin 12 γ/ml ozon uygulanımı ile 5 ve 10 dk içerisinde en etkin bakterisidal etkinin sağlandığı belirlendi. Ancak ilerleyen zamanlarda ozonun etkinliğini kaybettiği gözlendi. Bununla birlikte 40 γ/ml ozon uygulanımında bakterisidal etkinin zamana bağlı olarak arttığı ve 15 ile 30 dakikalarda bakterilerin tümünün öldüğü tespit edildi. Bu deneme sonunda Y. ruckeri'nin ozon gazına duyarlı olduğu gösterdiği bakterisidal etki ile belirlendi.

  İncele
• MakaleYalnız künye2017Scopus SJRQ4/r/munzur/3869
  Apoptotic effects of artificial feed supplemented with Thymus vulgaris on Oncorhynchus mykiss against Yersinia ruckeri

  Kucukgul, Altuğ; Kucukgul, Azime

  Chemical composition of the essential oil was analyzed by GC-MS, and found to contain the phenol (40.95 %) as major component. Results showed that Y. ruckeri (3 × 107cfu fish-1) infection caused the characteristic symptoms on rainbow trout (petechial hemorrhages on the surface of body and liver). Y. ruckeriinducedinhibitions of Cas-3, Cas-8, p53 and HSP-70. After the administration of thyme essential oil (10 mg kg-1 diet) to infected fish tissues, Cas-3, Cas-8, p53 and HSP-70 mRNAs were up-regulated by 9.31, 9.18, 9.58, and 6,1 fold change, respectively. According to the results of the study, the inhibitions seen all analyzed genes were due to Y. ruckeri infection. However, they were entirely increased by thyme treatment. Thus, targeting pro-apoptotic proteases by agents such as T. vulgaris could improve therapeutic options for infectious disease in fish.

  İncele
• MakaleAçık Erişim2012Scopus SJRQ2/r/munzur/4197
  Investigation of Yersinia ruckeri Infection in Rainbow Trout (Oncorhynchus mykiss Walbaum 1792) Farms by Polymerase Chain Reaction (PCR) and Bacteriological Culture

  Seker, Engin; Karahan, Murat; Ispir, Unal; Çetinkaya, Burhan; Saglam, Naim; Sarieyyüpoglu, Mustafa

  This study has been carried out to investigate the presence of Yersinia ruckeri, the causative agent of Enteric Red Mouth (ERM) disease, in rainbow trout fish breeding farms located in Elazig and Malatya provinces in Eastern Turkey. For this purpose, bacteriological culture and a specific polymerase chain reaction (PCR) have been compare with examine blood and intestine samples collected from adult and young rainbow trout fish as well as water samples collected from all the farms. Y. ruckeri was isolated and identified from eight of the 17 fish farms giving a proportion of 52.9% at fish farm. The disease was noted in five of the rainbow trout farms located in Elazig and three of the 11 rainbow trout farms located in Malatya. The agent was detected in five of the six young fish breeding farms. The isolation percentages of the causative agent at fish level were calculated as 25.7% (131/510) in adult and 31.7% (19/60) in young fish, giving an overall proportion of 26.3% (150/570). All the isolates have been successfully identified as Y ruckeri by species-specific PCR. No isolation could be made from water samples. It is therefore concluded that Y. ruckeri infection poses a significant threat to the fish farms in the study area and necessary precautions should urgently be taken in order to minimize economical losses due to ERM disease. A specific PCR can be utilized successfully in aquaculture for rapid identification of bacterial agents which will help to prevent spread of infectious diseases and will therefore contribute to the productivity of fishery sector.

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  AtıfWoS6·OpenAlex7

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