Use of Clove Oil and Eugenol to Anesthetize Fingerling Shabut Barbus grypus

In this study, the efficacy of four doses of clove oil and eugenol were compared to sedate fingerling Shabut Barbus grypus to various stages of sedation and recovery. The results from the present study indicated that effective concentrations were at 50L/L for eugenol (induction time: 58 8s; recovery time: 199 +/- 15s [mean +/- SD]) and clove oil (induction time: 76 +/- 6s; recovery time: 161 +/- 34s). The induction times decreased significantly with the increasing concentrations of clove oil and eugenol, while recovery times increased with increasing concentrations of anesthetic (P < 0.05). Exposure of fish to 75 or 100L/L clove oil or to 50, 75, or 100L/L eugenol resulted in mean induction times 1min. Fish did not become fully sedated within 3min when treated with 25L/L clove oil. Other concentrations of both anesthetics, fish reached to full induction at 3min and recovered from anesthesia 5min. Our results showed the clove oil and eugenol are effective anesthetics for fingerling Shabut when used at concentrations of 50-100L/L. Received March 25, 2013; accepted July 5, 2013