Sürdürülebilir Kalkınma Amaçları

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• MakaleAçık Erişim2026Scopus SJRQ1/r/munzur/4096
  Ellagic Acid Mitigates Lead (Pb)-Induced Toxicity in Cyprinus carpio: A Multi-Biomarker Assessment of Hematological, Immunological, and Oxidative Stress Responses Supported by Molecular Docking

  Eroglu, Mucahit; Cakmak, Mehmet Nuri; Pala, Aysegul; Uslu, Harun; Yonar, Serpil Mise; Ispir, Unal; Orhan, Cemal; Yonar, Muhammet Enis

  Lead (Pb) is a widespread environmental pollutant that induces systemic toxicity in aquatic organisms primarily through oxidative stress, hematological disruption, and immune dysfunction. This study investigated the protective effects of ellagic acid (EA) against Pb-induced toxicity in common carp (Cyprinus carpio) using a multi-biomarker approach and molecular docking. Fish were assigned to six experimental groups: control, EA-treated, Pb-I, Pb-I + EA, Pb-II, and Pb-II + EA. Fish in the Pb-I and Pb-II groups were exposed to 2.5 and 5 mg/L Pb, respectively, while EA was administered via diet at 100 mg/kg for 14 days. At the end of the exposure period, hematological indices, innate immune parameters, and oxidative stress biomarkers were assessed in blood, liver, kidney, and gill tissues. Pb exposure caused marked hematological impairment, suppressed immune responses, increased malondialdehyde levels, and disrupted antioxidant defense by reducing SOD, CAT, GSH-Px, and GSH levels while increasing GST activity. In contrast, dietary EA supplementation significantly mitigated Pb-induced alterations, improved hematological and immunological responses, reduced lipid peroxidation, restored antioxidant capacity, and normalized GST activity to control levels. Molecular docking analyses further showed that EA interacts with hemoglobin and immunoglobulin M, supporting its potential role in preserving oxygen transport and immune functions under Pb-induced stress. Overall, the findings demonstrate that Pb-induced toxicity involves coordinated disruption of redox homeostasis and immune function, whereas EA exerts a multi-target protective effect through biochemical and molecular mechanisms. This study provides mechanistic insight into chemical-biological interactions and supports the potential application of natural bioactive compounds in mitigating heavy metal-induced toxicity.

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  İncele
• MakaleAçık Erişim2026Scopus SJRQ2/r/munzur/898
  Cadmium-induced changes in haemato-immunological, oxidative stress and antioxidant parameters of Cyprinus carpio: Protective role of curcumin

  Pala, Ayşegül; Eroğlu, Mücahit; Mişe Yonar, Serpil; İspir, Ünal; Yonar, Muhammet Enis

  This study examined the adverse effects of sublethal concentrations of cadmium (Cd) and the potential of curcumin (CUR) to mitigate Cd-induced toxicity. We administered 1.5 and 3 mg/L of Cd and 100 mg/kg of CUR to fish (Cyprinus carpio) separately and in combination over 14 days. At the end of the experiments, various haematological and immunological parameters, oxidative stress levels and antioxidant parameters of fish were measured, and the differences between the groups were evaluated. Simultaneous CUR treatment with Cd reversed the changes in haematological parameters in fish, increased immune responses, reduced oxidative stress and normalized the impaired antioxidant enzyme activities. In summary, our study showed that dietary supplementation with CUR may be an effective strategy to reduce the toxic effects of Cd in carp.

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• MakaleAçık Erişim2025Scopus SJRQ4/r/munzur/2200
  Effects of chlorpyrifos on the freshwater mussel Unio elongatulus eucirrus (Bourguignat, 1860), with a focus on neurotoxicity and oxidative stress responses; [Efectos del clorpirifos en el mejillón de agua dulce Unio elongatulus eucirrus (Bourguignat, 1860), con especial atención a la neurotoxicidad y las respuestas al estrés oxidativo]

  Zorlar, Faruk; Şeker, Engin; Pala, Ayşegül

  This study set out to investigate the effects of the organophosphate insecticide chlorpyrifos (CPF) on certain biochemical parameters of the freshwater mussel Unio elongatulus eucirrus. Mussels were exposed to varying concentrations of CPF (10, 20, and 40 μg·L-1) for duration of 96 hours. Samples were collected from the mussels at the 24th and 96th hours of the experiment. The results indicated that exposure to CPF concentrations led to a decline in the activities of acetylcholinesterase (AChE), superoxide dismutase (SOD), catalase (CAT), and glutathione peroxidase (GPx) activities and, accompanied by an increase in malondialdehyde (MDA), and decreased reduced glutathione (GSH) levels in mussels. In conclusion, the present study demonstrates that CPF caused AChE inhibition, formation of oxidative stress, and negative effects on certain antioxidant parameters in the freshwater mussel U. elongatulus eucirrus.

  İncele
• MakaleYalnız künye2025Scopus SJRQ4/r/munzur/1914
  Bioaccumulation of copper by Gammarus pulex (L., 1758): monitoring through a statistical modelling approach

  Pala, Aysegul; Ince, Muharrem; Serdar, Osman

  The aim of this study is to test freshwater gammarids as ecotoxicological biomarkers. Gammarids were collected from fresh water and adapted to the laboratory environment. Then, the influence of various variables was investigated. For monitoring and detecting the bioaccumulation level in Gammarus pulex exposed to controlled copper ions and to evaluate potential human and ecological risks, the use of G. pulex as sentinel indicators of water quality is one rational approach. Copper bioaccumulation rate and fate are currently not sufficiently studied. For optimization, the experimental conditions to ensure bioaccumulation level and to find the effects of various variables on proses, a Box–Behnken experimental design was performed. Used mentioned model with statistical underpinnings for Cu2+ ions bioaccumulation have been analyzed, tested and verified and the viability of G. pulex as a biomonitor. The variables’ optimum levels (temperature; 18.1, pH; 4.3, initial concentration of Cu2+ ions, mg L−1; 11.2 and exposure time, h; 42.2) were achieved by employing the quadratic regression model. Critical role of G. pulex in freshwater biomonitoring and their potential to be evaluated as biomarkers for Cu2+ ions was confirmed. It was also explored to see if G. pulex may serve as a potential tool for estimating aquatic quality.

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  AtıfScopus1·WoS1·OpenAlex1

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• MakaleAçık Erişim2025Scopus SJRQ2/r/munzur/1451
  Determination of the Effect of Nanoparticle Copper on Navicula cryptocephala var. veneta by Biomarkers and Bioaccumulation Amounts

  Serdar, Osman; Aydın, Ayşe Nur; Ölçülü, Abdüllatif; Çiçek Çimen, Işıl Canan; Parlak Ak, Tuba; Derman, Taner; Pala, Ayşegül; Yıldırım, Nuran Cikcikoğlu

  Pollutants such as copper nanoparticles (CuNP), which form residues in the environment, especially in water environments, cause serious damage to the environment and the organisms living in the environment. In this study, Navicula cryptocephala var. veneta was selected as a model organism. The EC50 values of CuNP on Navicula cryptocephala var. veneta were determined as 4.94 mg\L and 3 different application concentrations were determined considering this ratio. The experiments were carried out in 3 replicates and samples were taken at 24 and 96 hours. At the end of 120 hours, samples were taken to determine the elimination values. CuNP accumulation amounts in the samples were measured by electrothermal atomic absorption spectrophotometer (ETAAS). For biomarker analysis, thiobarbituric acid (TBARS) and glutathione (GSH) levels and superoxide dismutase (SOD), catalase (CAT) and glutathione peroxidase (GPx) activities were performed using CAYMAN ELISA kit. According to the study data, as CuNP concentration and application time increased, the amount of accumulation in Navicula cryptocephala also increased. Increasing-decreasing changes were observed in elimination. CuNP caused oxidative stress in N. cryptocephala and caused changes in TBARS and GSH levels. In addition, it caused increases in SOD activities and statistically significant decreases (P<0.05) in CAT and GPx activities.

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  AtıfScopus2·WoS1

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• MakaleAçık Erişim2022/r/munzur/6506
  Güney Doğu Anadolu Bölgesi Sülük Faunasının Araştırılması

  Pala, Ayşegül; Dörücü, Mustafa; Saglam, Naim; Sesli, Ahmet; Özbay, Özkan; Balci, Mustafa; Kılıç, Ali; Özbey, Nurten; Demir, Timur; Arca, Selami

  Bu çalışma, Ocak 2012- Aralık 2014 tarihleri arasında Güney Doğu Anadolu Bölgesinde yer alan yedi ilde (Adıyaman, Batman, Diyarbakır, Gaziantep, Kilis, Mardin ve Şanlıurfa) bulunan akarsu, baraj, göl, gölet ve sazlık alan olmak üzere toplam 169 tatlısu habitatında sülük faunasının belirlenmesi amacıyla yürütüldü. Sülük örnekleri habitatın özelliğine göre birim zaman, birim çerçeve (Sülük toplama çerçevesi) ve Ekman kepçesi yöntemiyle toplandı. Toplam 169 sulak alanın 53’ünde sülük tespit edildi. Çalışma süresince toplanan sülük örnekleri su bulunan 0,5-1,0 L’lik pet bidonlarla laboratuvara taşındı. Sülük örnekleri önce % 10 etanol içinde bayıltıldı, sonra %70 alkol veya %4 formalin içinde tespit edildi. Toplanmış olan sülükler morfolojik özelliklerine göre stereo mikroskop altında teşhisi yapıldı. Bu çalışma sonunda , Güneydoğu Anadolu Bölgesi sulak alanlarında Albolossiphonia heteroclita, Albolossiphonia sp., Dina apathyi, Dina sp., Erpobdella octoculata, E. testacea, Erpobdella sp., Helobdella stagnalis, Hirudo sulukii, Placobdella costata ve Placobdella sp. olmak üzere altı soya ait toplam 11 farklı sülük türü belirlendi. Söz konusu çalışma kapsamında tesbit edilen H. sulukii dünya için 2016 yılında tanımlanmış bir tıbbi sülük türü olup, bölge halkı tarafından geleneksel ve tamamlayıcı tıp uygulamaları çerçevesinde kullanılmaktadır.

  İncele
• MakaleYalnız künye2022/r/munzur/5872
  Güney Doğu Anadolu Bölgesi Sülük Faunası Üzerine Araştırmalar

  Özkan, Kadir; Demir, Timur; Balci, Mustafa; Sağlam, Naim; Dörücü, Mustafa; Özbey, Nurten; Kılıç, Ali; Pala, Ayşegül; Sesli, Ahmet; Özbay, Özkan

  Bu çalışma, Ocak 2012- Aralık 2014 tarihleri arasında Güney Doğu Anadolu Bölgesinde yer alan yedi ilde (Adıyaman, Batman, Diyarbakır, Gaziantep, Kilis, Mardin ve Şanlıurfa) bulunan akarsu, baraj, göl, gölet ve sazlık alan olmak üzere toplam 169 tatlısu habitatında sülük faunasının belirlenmesi amacıyla yürütüldü. Sülük örnekleri habitatın özelliğine göre birim zaman, birim çerçeve (Sülük toplama çerçevesi) ve Ekman kepçesi yöntemiyle toplandı. Toplam 169 sulak alanın 53’ünde sülük tespit edildi. Çalışma süresince toplanan sülük örnekleri su bulunan 0,5-1,0 L’lik pet bidonlarla laboratuvara taşındı. Sülük örnekleri önce % 10 etanol içinde bayıltıldı, sonra %70 alkol veya %4 formalin içinde tespit edildi. Toplanmış olan sülükler morfolojik özelliklerine göre stereo mikroskop altında teşhisi yapıldı. Bu çalışma sonunda , Güneydoğu Anadolu Bölgesi sulak alanlarında Albolossiphonia heteroclita, Albolossiphonia sp., Dina apathyi, Dina sp., Erpobdella octoculata, E. testacea, Erpobdella sp., Helobdella stagnalis, Hirudo sulukii, Placobdella costata ve Placobdella sp. olmak üzere altı soya ait toplam 11 farklı sülük türü belirlendi. Söz konusu çalışma kapsamında tesbit edilen H. sulukii dünya için 2016 yılında tanımlanmış bir tıbbi sülük türü olup, bölge halkı tarafından geleneksel ve tamamlayıcı tıp uygulamaları çerçevesinde kullanılmaktadır.

  İncele
• MakaleAçık Erişim2022Scopus SJRQ2/r/munzur/3079
  Effect of chlorpyrifos on some biochemical changes in Cyprinus carpio: the protective effect of ellagic acid

  Mişe Yonar, Serpil; Yonar, Muhammet Enis; Ural, Mevlüt Şener; Pala, Ayşegül

  In this study, ameliorative effects of ellagic acid (EA) on oxidative stress induced by chlorpyrifos (CPF) in carp, Cyprinus carpio, were investigated. Fish were divided into six groups: C (no treatment), EA (100 mg kg fish−1), CPF-1 (0.040 mg L−1), CPF-2 (0.080 mg L−1), CPF-1 + EA, and CPF-2 + EA. CPF and EA were applied simultaneously for 14 days and, at the end of the study, liver, kidney, and gill samples were collected from fish. On the taken tissue samples, malondialdehyde (MDA) level and some antioxidant enzyme (superoxide dismutase (SOD), catalase (CAT), glutathione peroxidase (GPx), and glutathione-S-transferase (GST)) activities were evaluated. The results demonstrated statistically significant increases in the MDA levels of the CPF-1 and CPF-2 groups. On the other hand, the MDA levels were significantly decreased by EA administration. Also, CPF exposure caused statistically significant increases in the SOD and GST activities and statistically significant decreases in the CAT and GPx activities. However, treatment with EA reversed negative alterations in the SOD, CAT, GPx, and GST activities. Therefore, the results of this study results showed that simultaneous treatment with EA alleviates CPF-induced oxidative stress in fish.

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  AtıfScopus8·WoS10·OpenAlex10

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• MakaleAçık Erişim2021Scopus SJRQ1/r/munzur/2748
  Ameliorative effect of Fennel (Foeniculum vulgare) essential oil on chlorpyrifos toxicity in Cyprinus carpio

  Pala, Ayşegül; Serdar, Osman; Mişe Yonar, Serpil; Yonar, Muhammet Enis

  Chlorpyrifos (CPF) is an organophosphate pesticide that is frequently and widely used to control both agricultural and domestic pests worldwide. In this study, the protective effect of Fennel (Foeniculum vulgare) essential oil (FEO) was investigated in carp (Cyprinus carpio) exposed to CPF. The fish were divided into six groups that one control group (no treatment) and five experimental groups (FEO (3ml/100g diet) group, CPF1 (0.023 mg/l) group CPF2 (0.046 mg/l) group, CPF1 (0.023 mg/l) plus FEO (3ml/100g diet) group, CPF2 (0.046 mg/l) plus FEO (3ml/100g diet) group). Blood and tissue (liver, kidney, gill, and brain) samples were taken from the fish at the end of 14 days of application. Hemoglobin (Hb) level, nitoblue tetrazolium (NBT) activity, and total immunoglobulin (TI) level were measured in blood samples of fish. Acetylcholinesterase (AChE) activity was determined in brain tissue while malondialdehyde (MDA) level, reduced glutathione (GSH) level, catalase (CAT), and glutathione peroxidase (GPx) activity were determined in liver, kidney, and gill tissues. The results showed that there was a significant decrease in Hb level, NBT activity, and TI levels in CPF-treated fish compared to the control group. In addition, increased in MDA levels and significant decreases in GSH level, AChE, CAT, and GPx activities were observed in CPF-treated groups. However, FEO-treated was showed a significant improvement in all parameters except AChE activity compared to CPF groups. These study findings showed that FEO could improve CPF-induced toxicity in C. carpio, except inhibition of AChE activity.

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  AtıfScopus6·WoS6·OpenAlex8

  İncele
• MakaleAçık Erişim2020/r/munzur/6816
  Malathionun Gammarus pulex (Tatlı Su Amphipodu)’te Asetilkolinesteraz Aktivitesi Üzerine Akut Etkisi

  Serdar, Osman; Pala, Ayşegül; Aydin, Rahmi

  Bu çalışmada, organofosfat insektisit malathionun subletal konsantrasyonlarının Gammarus pulex'in AChE aktivitesi üzerine akut (24 ve 48 saat) etkisi incelenmiştir. Bu amaçla, G. pulex'e malathionun iki farklı sublethal konsantrasyonu (0.1 ve 0.2 mg l-1 ) 24 ve 48 saat boyunca uygulandı. 24 ve 48 saat malathion maruziyetinin sonunda G. pulex örnekleri alındı. Alınan örneklerde AChE enzim aktivitesi ve protein düzeyi belirlenerek, spesifik AChE enzim aktivitesi hesaplandı. Malathionunun sublethal konsantrasyonları G. pulex'te AChE aktivitesininin zamana bağlı artan inhibisyonuna neden oldu. 0.1 mg l-1 malathion konsantrasyonuna maruz kalan grupta, AChE'nin inhibisyonu, kontrol ile karşılaştırıldığında, 24 saatte %50 ve 48 saatte %74 olarak tespit edildi. Benzer şekilde, 0.2 mg l-1 malathion konsantrasyonuna maruz kalan grupta da kontrol ile karşılaştırıldığında sırasıyla 24 ve 48 saatte % 60 ve % 68 AChE inhibisyonu gözlendi. Sonuç olarak, G. pulex'in malathiona 24 ve 48 saat boyunca maruz kalması AChE aktivitesinin yüksek oranda inhibe edilmesine neden olmuştur. Ayrıca, sonuçlar G.pulex'teki % 74'e kadar AChE inhibisyon seviyelerinin akut ölüme neden olmadığını ve G. pulex'teki AChE aktivitesinin ölçümünün akut malathion maruziyetinin ve etkilerinin biyobelirteci olacağını göstermektedir.

  AtıfOpenAlex3·TR Dizin1

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