Anaesthetic Effects of Rosewood (Aniba rosaeodora) Essential Oil and 2-phenoxyethanol on Goldfish (Carassius auratus) at Different Water Temperatures

In this study, it was aimed to investigate the anesthetic effects of rosewood essential oil and 2- phenoxyethanol in goldfish at different water temperatures. Anesthetic trials were carried out at water temperatures of 14°C and 20°C. Concentrations of 250, 300 and 350 μl·L-1 for rosewood essential oil and 600, 700 and 800 μl·L-1 for 2-phenoxyethanol were tested. The ideal rosewood essential oil concentration was determined as 300 μl·L-1 at 14°C and 250 μl·L-1 at 20°C. The ideal concentration for 2-phenoxyethanol was determined to be 800 μl·L-1 at both water temperatures. Higher concentrations of rosewood essential oil and 2-phenoxyethanol caused shorter induction times and prolonged recovery times. Concentrations of anesthetic agents were negatively correlated with induction times and positively correlated with recovery times. A weak negative relationship was found between deep anesthesia and full recovery durations at 14ºC and a negative relationship at 20ºC for rosewood essential oil. A negative relationship was detected between deep anesthesia and full recovery times in 2-phenoxyethanol at 14ºC and 20ºC. There is a strong negative relationship between rosewood essential oil deep anesthesia times and rosewood essential oil concentrations at 14ºC and 20ºC, and a similar relationship was found for 2-phenoxyethanol. However, a strong positive relationship was detected between full recovery times and concentrations of both anesthetic agents at different water temperatures. As a result of increasing water temperature, anesthesia and recovery times were observed to be shortened for both anesthetic substances. The interaction of water temperature and anesthetic concentration was found to be important in the induction and recovery durations for rosewood essential oil and 2-phenoxyethanol.