Enzymatic and Bibliometric Analysis of Bifidobacterium sp. of Probiotic Origin in The Based Digestive Tract of Honey Bees

Honey bees (Apis mellifera) are a unique super organism that produces products such as honey, pollen, royal jelly, propolis, beeswax and bee venom and serves as a vector for pollination of many natural and industriyal plants. The effect of intestinal microflora and, accordingly, the immune system come to the fore among the causes of colony losses that have occurred recently. The diversity and densities of bacteria in the digestive tube of bees are affected by their immune systems. In order for this effect to be positive, the intestinal microflora must be natural and balanced. The enzyme activity of the probiotic-derived Bifidobacterium sp., which has an important place among the bacterial populations in the intestine, has a critical effect on both other symbiont microorganisms and the nutrition of the host. Therefore, in this study, both glikozide hidrolase enzyme activity analysis and bibliometric analysis were performed to emphasize the importance of Bifidobacterium sp. Different pH, temperature and substrates were preferred to calculate the optimum enzyme activity. As a result of the analysis of the enzyme activity, the glycoside hydrolase activity (pH 5.0 and 30oC) of Bifidobacterium sp. was determined. Further use of inulin substrate has shown that it is a natural prebiotic source for this probiotic microorganism. At the same time, the hydrolysis of xylan, which is known as a new generation prebiotic source, has also taken place. For bibliometric analysis, correlation links in Web of Science and Scopus database were concluded with the help of VOS viewer software