Sürdürülebilir Kalkınma Amaçları
Yayınlar
- MakaleAçık Erişim2022Scopus SJRQ3Effects of kefir supplementation on body antioxidant-systems, haemolymph heat-shock protein 70 and trypsin, in the crayfish Astacus leptodactylus Eschscholtz (Decapoda, Astacidea)
Aksu, Önder; Türkoǧlu, Semra; Can, Erkan; Erişir, Mine; Kocabaş, Mehmet; Kayim, Murathan; Benzer, Fulya
This study was designed to investigate the effects of dietary kefir on trypsin, heat shock protein 70 (HSP70) as well as the oxidant and antioxidant status of the freshwater crayfish, Astacus leptodactylus. The experiment was performed with the four following treatments: Control group, D1, D2 and D3 (kefir supplemented diet 2, 4, 8 g/kg of crayfish body mass, respectively). Crayfish were stocked into 12 fibreglass tanks (three replicates per treatment) and fed for 45 days. Reduced glutathione (GSH), glutathione peroxidase (GSH-Px), malondialdehyde (MDA) and catalase (CAT) in hepatopancreas and muscle were analysed to determine the status of the oxidant/antioxidant balance. In addition, HSP70 and trypsin were measured in haemolymph. Our results indicated that the activity of GSH-Px, MDA and GSH levels decreased in hepatopancreas ( p > 0.05) and muscle tissues ( p < 0.05) with increasing kefir concentration, while CAT activity increased ( p < 0.05). Trypsin and HSP70 insignificantly decreased in haemolymph.
AtıfScopus1WoS1OpenAlex1
İncele - MakaleYalnız künye2015Scopus SJRQ1Effect of semen extender supplementation with cysteine on postthaw sperm quality, DNA damage, and fertilizing ability in the common carp (Cyprinus carpio)
Ögretmen, Fatih; Inanan, Burak Evren; Kutluyer, Filiz; Kayim, Murathan
Amino acids have an important biological role for prevention of cell damage during cryopreservation. The objective of this study is to determine the effects of cysteine on postthaw sperm motility, duration of sperm motility, DNA damage, and fertility in the common carp (Cyprinus carpio). Sperm collected from 10 individuals was cryopreserved in extenders containing different cysteine concentrations (2.5, 5, 10, and 20 mM). Semen samples diluted at the ratio of 1:9 by the extenders were subjected to cryopreservation. After dilution, the semen was aspirated into 0.25-mL straws; the straws were placed on the tray, frozen in nitrogen vapor, and plunged into liquid nitrogen. DNA damage was evaluated by comet assay after cryopreservation. Our results indicated that an increase in the concentration of cysteine caused a significant increase in the motility rate and duration of sperm in the common carp (C carpio; P < 0.05). Comparing all concentrations of cysteine, the best concentration of cysteine was 20 mM. Higher postthaw motility (76.00 +/- 1.00%) and fertilization (97.00 +/- 1.73%) rates were obtained with the extender at the concentration of 20 mM. Supplementation of the extender with cysteine was increased the fertilization and hatching rate and decreased DNA damage. Consequently, cysteine affected the motility, fertilization, and DNA damage positively, and extenders could be supplemented with cysteine. (C) 2015 Elsevier Inc. All rights reserved.
AtıfWoS48OpenAlex54
İncele - Proje2014Gökkuşağı alabalığı (oncorhynchus mykiss) nın sperm kriyoprezervasyonunda (dondurulması) antioksidanların kullanılması, sperm kalite parametrelerinin, yağ asidi, protein profillerinin ve seminal plazma kompozisyonunun belirlenmesi
Yavas, Ilker; Öğretmen, Fatih; Kayim, Murathan; Tuncer, Pürhan Barbaros
-
- MakaleAçık Erişim2014Scopus SJRQ1Cryopreservation of rainbow trout Oncorhynchus mykiss spermatozoa: Effects of extender supplemented with different antioxidants on sperm motility, velocity and fertility
Kutluyer, Filiz; Kayim, Murathan; Ögretmen, Fatih; Büyükleblebici, Serhat; Tuncer, P. Barbaros
In present study, it was examined whether addition of different antioxidants to the cryopreservation extenders had an effect on semen post-thaw fertility and motility in rainbow trout (Oncorhynchus mykiss) and also it was investigated the sperm characteristics post-thaw sperm characteristics and fertility. The collected semen was pooled to minimize individual variation. Each pooled ejaculate was split into 12 equal aliquots and diluted with base extenders supplemented with the antioxidants, and a base extender with no additives (control). The pooled semen samples diluted at the ratio of 1:10 by the extenders were subjected to cryopreservation. Antioxidants were separately added to the extenders (one per experimental group): catalase (250 U/l), superoxide dismutase (250 U/l), peroxidase (250 U/l), oxidized glutathione (1.5 mmol/l), reduced glutathione (1.5 mmol/l), L-methionine (1.5 mmol/l), uric acid (0.25 mmol/l), L-ascorbic acid (0.5 mmol/l), alpha-tocopherol (2.0 mmol/l), beta-carotene (0.5 mmol/l) and camitine (0.5 mmol/l). After dilution the semen was aspirated into 0.25 ml straws, the straws were placed on the tray, frozen for 10 min, and plunged into liquid nitrogen. Our results indicated that the post-thaw motility rate increased in extenders supplemented with uric acid, L-methionine, SOD, L-carnitine, alpha-tocopherol and L-reduced glutathione (p < 0.05). The motility duration of frozen thawed semen increased in extenders supplemented with uric acid, L-methionine, SOD, alpha-tocopherol and L-reduced glutathione (p < 0.05). Fertilization rate and hatching rate of frozen-thawed semen was not affected by the tested antioxidants. Consequently, the tested antioxidants affected the motility parameters and cryopreservation extenders could be supplement with antioxidants. This study suggested usage of antioxidants in the cryopreservation of rainbow trout. (C) 2014 Elsevier Inc. All rights reserved.
AtıfWoS76OpenAlex92İncele - MakaleAçık Erişim2014Scopus SJRQ3EFFECT OF DIETARY KEFIR ON THE DIGESTIVE AND LIVER ENZYMES ACTIVITIES AND GLUCOSE LEVEL OF CORUH TROUT, SALMO CORUHENSIS (ACTINOPTERYGII: SALMONIFORMES: SALMONIDAE)
Can, Safak Seyhaneyildiz; Kutluyer, Filiz; Can, Erkan; Kayis, Sevki; Sonay, Fatma Delihasan; Köse, Ozay; Aksu, Onder; Erdamar, Husamettin; Yigitoglu, M. Ramazan; Kayim, Murathan
The probiotics are very important for aquaculture because of their role in enhancing the fish health status, improving disease resistance, growth performance, and body composition, reducing malformations, improving gut morphology and the overall microbial balance. The objective of the presently reported study was to investigate effects of kefir as a probiotic product on the digestive and hepatic enzyme activities, as well as glucose level of Coruh trout, Salmo coruhensis Turan, Kottelat et Engin, 2010. The experiment was performed with four treatments: control group (no kefir), D1, D2, and D3 (kefir-supplemented diet: 10, 20, and 40 g . kg(-1) fish body mass, respectively). The activity of: alanine aminotransferase (ALT), aspartate aminotranferase (AST), lactate dehydrogenase (LDH), lipase, amylase-as well as the glucose levels were determined after 4 months. The results indicated that glucose levels (P < 0.05) and digestive enzymes (lipase and amylase) in the blood serum decreased in fish fed diets containing kefir (P > 0.05). On the contrary, the activity of liver enzymes (ALT, AST, and LDH) increased insignificantly (P > 0.05). In conclusion, digestive and liver enzyme activities were affected by quantitative changes in dietary kefir insignificantly but further research is needed to establish whether the liver and digestive enzyme physiology of Coruh trout can be affected by kefir-supplemented diets in a long-term trial.
AtıfWoS6OpenAlex12İncele - MakaleYalnız künye2014Scopus SJRQ3Use of Clove Oil and Eugenol to Anesthetize Fingerling Shabut Barbus grypus
Ögretmen, Fatih; Gölbasi, Selami; Inanan, Burak E.; Kizak, Volkan; Kayim, Murathan
In this study, the efficacy of four doses of clove oil and eugenol were compared to sedate fingerling Shabut Barbus grypus to various stages of sedation and recovery. The results from the present study indicated that effective concentrations were at 50L/L for eugenol (induction time: 58 8s; recovery time: 199 +/- 15s [mean +/- SD]) and clove oil (induction time: 76 +/- 6s; recovery time: 161 +/- 34s). The induction times decreased significantly with the increasing concentrations of clove oil and eugenol, while recovery times increased with increasing concentrations of anesthetic (P < 0.05). Exposure of fish to 75 or 100L/L clove oil or to 50, 75, or 100L/L eugenol resulted in mean induction times 1min. Fish did not become fully sedated within 3min when treated with 25L/L clove oil. Other concentrations of both anesthetics, fish reached to full induction at 3min and recovered from anesthesia 5min. Our results showed the clove oil and eugenol are effective anesthetics for fingerling Shabut when used at concentrations of 50-100L/L. Received March 25, 2013; accepted July 5, 2013
AtıfWoS10OpenAlex14İncele - MakaleYalnız künye2013Scopus SJRQ3Triploit ve diploit dere alabalıklarında (Salmo trutta fario L, 1758) büyüme performansı, gonat yapısı ve eritrosit boyutunun karşılaştırılması
Kizak, Volkan; Türel, Murat; Güner, Yusuf; Kayim, Murathan
The aim of this study was to compare growth, gonadal structure and red blood cell sizes of triploid and diploid brown th trout (Salmo trutta fario). Triploidy was induced by providing a heat shock treatment to the fertilized eggs. From the 18month post-fertilization triploid (TBT) and control diploid (CBT) brown trout groups were investigated up to 32 nd month. Survival, specific growth rate, feed conversion ratio, condition factor, relative growth rate, absolute growth rate, gonadosomatic index, hepatosomatic index and carcass yield were determined in both groups. It has been determined that the triploid fish showed greater live weight than diploid fish despite the difference was not significant (P>0.05). In triploid group triploidisation success was obtained as 95% and male/female ratio was found as 44.4-55.6%. The red blood cells were significantly larger in triploids than diploid ones (P<0.05). Regarding histological section of gonads, triploidy resulted in reduced gonadal development in female, while triploid males seem to exhibit normal gonadal development. Our results suggest that since triploid females could grow more than males, the growth performance studies on triploid brown trout should be performed with all-female stocks under fish farming practices especially for the production of large size trout.
AtıfTR Dizin3
İncele - MakaleYalnız künye2013Scopus SJRQ3The Effect of Different Feeding Protocols on Compensatory Growth of Black Sea Trout Salmo trutta labrax
Kocabas, Mehmet; Basçinar, Nadir; Kayim, Murathan; Er, Halil; Sahin, Halit
The objective of this study was to determine the effects of different feeding protocols on compensatory growth, feeding rate, and feed conversion efficiency of Black Sea Trout Salmo trutta labrax. To our knowledge, no previous feeding experiments with Black Sea Trout have incorporated a fasting period to simulate the handling and acclimation conditions experienced by farmed fish. Fifteen fish per tank (10.69 +/- 0.06cm and 13.22 +/- 0.14g, mean +/- SE) were stocked into 40-L fiberglass tanks. Fish were fed with different fasting-feeding regimes for 95 d and were equally allotted to four treatments (T-cont: control; T5-10: 5 d fasting, 10days feeding; T10-10: 10 d fasting, 10 d feeding; and T15-10: 15 d fasting, 10 d feeding) with three replicates per treatment. The results indicated that length (P = 0.0005), weight (P = 0.000), condition factor (P = 0.013), and specific growth rate (P = 0.014) were significantly affected by the interaction between feeding and time. All fasting treatments showed partial compensation during refeeding. There was an increase in daily feeding rate and feed conversion efficiency in fasting treatments compared with the control treatment. In contrast, at the end of the experiment specific growth rate, condition factor, and body weight in fasting treatments were significantly lower compared with the control treatment. We concluded that there was partial compensation of growth with regular refeeding after periods of feed deprivation (e.g., 5, 10, or 15 d) over a long term and a shorter fasting period may be preferred in order to achieve compensatory growth. Received November 9, 2012; accepted April 9, 2013
AtıfWoS6OpenAlex7İncele - MakaleYalnız künye2013Scopus SJRQ3Comparison of Growth Performance, Gonadal Structure and Erythrocyte Size in Triploid and Diploid Brown Trout (Salmo trutta fario L, 1758)
Kizak, Volkan; Güner, Yusuf; Türel, Murat; Kayim, Murathan
The aim of this study was to compare growth, gonadal structure and red blood cell sizes of triploid and diploid brown trout (Salmo trutta fario). Triploidy was induced by providing a heat shock treatment to the fertilized eggs. From the 18th month post-fertilization triploid (TBT) and control diploid (CBT) brown trout groups were investigated up to 32nd month. Survival, specific growth rate, feed conversion ratio, condition factor, relative growth rate, absolute growth rate, gonadosomatic index, hepatosomatic index and carcass yield were determined in both groups. It has been determined that the triploid fish showed greater live weight than diploid fish despite the difference was not significant (P>0.05). In triploid group triploidisation success was obtained as 95% and male/female ratio was found as 44.4-55.6%. The red blood cells were significantly larger in triploids than diploid ones (P<0.05). Regarding histological section of gonads, triploidy resulted in reduced gonadal development in female, while triploid males seem to exhibit normal gonadal development. Our results suggest that since triploid females could grow more than males, the growth performance studies on triploid brown trout should be performed with all-female stocks under fish farming practices especially for the production of large size trout.
AtıfWoS24OpenAlex22İncele - MakaleYalnız künye2013Scopus SJRQ3Seasonal Variations of Proximate and Total Fatty Acid Composition of Wild Brown Trout in Munzur River, Tunceli-Turkey
Ates, Mehmet; Çakirogullari, Gul Celik; Kocabas, Mehmet; Kayim, Murathan; Can, Erkan; Kizak, Volkan
Seasonal variations of proximate composition and fatty acid profiles of wild brown trout (Salmo trutta macrostigma Dumeril, 1858) caught from the Munzur River, Tunceli-Turkey were investigated in this study. It was determined that wild brown trout was high in mono-unsaturated, poly-unsaturated and saturated fatty acids, respectively. C18:1 omega-9 (oleic acid) was the most abundant fatty acid in all seasons ranging from 20.27 to 29.35 %. C22:6 omega-3 (docosahexaenoic acid-DHA), C18:3 omega-3c (cis-linolenic acid), C18:2 omega-6c (linoleic acid), and C20:5 omega-3 (eicosapentaenoic acid-EPA) were found to be predominant fatty acids in PUFAs. As a conclusion, wild brown trout living in Munzur river could be considered as an important protein and omega-3 fatty acid source according to the nutritional quality results evaluated in terms of proximate and fatty acid composition.
AtıfWoS13OpenAlex11İncele - MakaleYalnız künye2012Scopus SJRQ2Determination of Bioaccumulation of Heavy Metals and Selenium in Tissues of Brown Trout Salmo trutta macrostigma (Dumeril, 1858) from Munzur Stream, Tunceli, Turkey
Can, Erkan; Yabanli, Murat; Kehayias, George; Aksu, Onder; Kocabas, Mehmet; Demir, Veysel; Kayim, Murathan; Kutluyer, Filiz; Seker, Sebahat
AtıfOpenAlex22İncele - MakaleAçık Erişim2012Scopus SJRQ3The Effects of Different Dosage of Kefir with Different Durations on Growth Performances and Antioxidant System in the Blood and Liver Tissues of Coruh Trout (Salmo coruhensis)
Can, Erkan; Kurtoglu, Ilker Zeki; Benzer, Fulya; Erisir, Mine; Kocabas, Mehmet; Kizak, Volkan; Kayim, Murathan; Çelik, Huseyin Tugrul
The objective of the present work is to examine the effects of different doses (0, 10, 20 and 40 g kg(-1) fish diet mass) of kefir on growth performance and oxidant-antioxidant status in the blood and liver tissues of Coruh trout, Salmo coruhensis, in different periods (2 and 3 months). In this study, survival was more than 88.3%, and irrespective of dietary kefir levels at the end of the study. There were no significant differences in SGR (Specific growth rate), FCR (Feed conservation rate), CF (Condition factor) among fish fed diets with 0, 10, 20 and 40 g kg(-1) kefir, however, these growth values were significantly different in terms of durations between 2-month and 3-month treatment (P<0.05). Our results indicated that TAS (Total Antioxidant Status) and TOS (Total Oxidant Status) in blood tissues reduced with kefir treatment at the end of third month (P<0.05). Malondialdehyde (MDA) levels in liver decreased in all groups compared with control group and a considerable extent decrease was observed in 40 g dose of kefir treatment at the end of third month. The data obtained from this experiment indicated that the same dose of kefir was more effective at the end of 3-month treatment than 2-month treatment (P<0.001). Although there was no statistical difference among groups, an increase in the glutathione peroxidase (GSH-Px) enzyme activity was observed in all groups compared to control groups. While catalase (CAT) activity decreased in all groups compared to control group at the end of second month (P<0.01), the decrease was insignificant level at the end of third month. In glutathione (GSH) levels, statistical differences were no observed in all groups compared to the control group with 2-month treatment while there were significant increases with 3-month treatment (P<0.001). It was concluded that kefir could play an antioxidant role and its effectiveness depended on dosage and time of application in Coruh trout, S. coruhensis.
AtıfWoS15OpenAlex15İncele - MakaleYalnız künye2011Scopus SJRQ3Proximate Composition, Fatty Acid Profile and Mineral Content of Wild Brown Trout (Salmo trutta sp.) From Munzur River in Tunceli, Turkey
Kayim, Murathan; Öksüz, Abdullah; Özyilmaz, Ayse; Kocabas, Mehmet; Can, Erkan; Kizak, Volkan; Ates, Mehmet
In present study, the proximate composition, fatty acid profile and mineral content of wild brown trout living in Munzur river (Tunceli/Turkey) were investigated. The average level of protein, lipid, moisture and ash content of muscle of the wild brown trout was calculated to be 17.48, 2.3, 77.8 and 1.5 %, respectively. Thirty fatty acids were identified in this study. Identification of the fatty acid was carried out with gas chromatography-mass spectrometry (GC-MS). The total polyunsaturated fatty acids (PUPA) were found the highest fatty acid followed by that of saturated fatty acids (SFA) and monounsaturated fatty acids (MUFA). Docosahexaenoic acid (DHA, C22:6n3) and eicosapentaenoic acid (EPA, C20:5n3), linolenic acid (LNA, C18:3n3) and linoleic acid (LA, C18:2n6) were found to be predominant fatty acids in PUFAs with the percentages of 11.25, 6.82, 6.30 and 4.88, respectively. The ratio of n3/n6 and DHA /EPA was calculated to be 4.55 and 1.65, respectively. Twelve elements were determined in this study. Determination of elements was performed with ICP-AES. P and K were the predominant element among the minerals analyzed and calculated to be 1305.56-2967.06 and 1908.68-1220.10 mg kg(-1) in muscle-skin of the wild brown trout, respectively.
AtıfWoS8İncele - MakaleAçık Erişim2011Scopus SJRQ3Comparison of the survival and growth performance in rainbow trout (Oncorhynchus mykiss) and brown trout (Salmo trutta fario) fry
Kizak, Volkan; Guner, Yusuf; Turel, Murat; Can, Erkan; Kayim, Murathan
Rainbow trout is an intensively cultured species because of their more cultivable character than brown trout. Culture of brown trout could not be expanded due to low growth performance of brown trout compared to rainbow trout. This experiment was conducted in a commercial trout farm, Aegean area (Turkey). Growth performances and survivals of rainbow and brown trouts from fry to fingerling was observed for 155 days. Growth performances, feed conversion rates (FCR) and survivals were determined. Initial weights of rainbow and brown trouts were 0.1 +/- 0.01 g. Final weights were 26.5 +/- 5.19 and 12.97 +/- 2.74 g, respectively at the end of the experiment. Survival and FCR of rainbow and brown trouts were 83.9, 80 and 0.59, 0.61% respectively. As a result, there is a similarity between these two trout species in point of survivals and FCR's although, growth performance was obviously better than brown trout in early development of rainbow trout.
AtıfWoS5OpenAlex16İncele - MakaleYalnız künye2011Scopus SJRQ3A comparison of the survival and growth performance in rainbow trout (Oncorhynchus mykiss) and brown trout (Salmo trutta fario) fry
Kizak, Volkan; Guner, Yusuf; Turel, Murat; Can, Erkan; Kayim, Murathan
The rainbow trout is an intensively cultured species because of its more cultivatable character than the brown trout. In this experiment, the brown trout culture did not expand due to low growth performance compared to that of the rainbow trout. This experiment was conducted in a commercial trout farm, Aegean Area (Turkey). Growth performances and survivals of rainbow and brown trouts from fry to fingerling were observed for 155 days. Growth performances, feed conversion rates and survivals were determined. The initial weights of the rainbow and brown trouts were 0.1 +/- 0.01 g. Final weights were 26.5 +/- 5.19 g and 12.97 +/- 2.74 g, respectively at the end of experiment. Survival and FCR of rainbow and brown trouts were 83.9 and 80%; 0.59 and 0.61 respectively. As a result, there is a similarity between these two trout species in point of survivals and FCR's although growth performance of the rainbow trout was significantly better than that of the brown trout.
AtıfWoS4İncele - MakaleYayıncıda açık erişim2010Scopus SJRQ3The Effects of Different Feeds under the Same Salinity Conditions on the Growth and Survival Rate of Artemia
Kayim, Murathan; Ates, Mehmet; Elekon, Hasan Alper
This study reports effects of 4 different feeds under same salinity conditions on feeding of anemia in terms of growth and survival rate. The feeds comprised of four groups (Group A: -16% Selco + 80% Headstart + 4% Molasses mixture feed; Group B: -85% Chlorella + 15% Molasses mixture feed; Group C: 50% Chlorella + 50% Headstart mixture feed and Group D: -100% Chlorella feed). Through stabilizing temperature, oxygen, pH and salinity values as general water parameters and replacing 10% sea water from the tank on daily basis, the survival rate and the growth in length of the anemia were analysed as well as the changes in nitrite content in the water. The results showed that the highest increase of 1400 mu in anemia with average pre-test length of 450 mu was observed on feed C. The highest rate of 58.33% survival was recorded on feed D. Similarly, the highest nitrite of 1.50 mg L-1 was measured in trial tank containing artemia fed on feed A and least nitrite generation of 0.45 mg L-1 was noted in trial tank containing anemia fed on feed C.
AtıfWoS2İncele - MakaleYalnız künye2010Scopus SJRQ3Comparing the Effectiveness of Ovopel and Carp Pituitary Extract (CPE) on Artificial Spawning of Scaly Carp (Cyprinus carpio)
Kayim, Murathan; Bozkurt, Yusuf; Ogretmen, Fatih
The effect of ovulation stimulators (Ovopel and carp pituitary extract) on spawning of scaly carp females were investigated. Following application of Ovopel, eggs were obtained from higher percentage of females than CPE treatment (92.5 and 75.0%, respectively. In the case of Ovopel stimulator, female weights were significantly correlated with living embryo (r = 0.576, p<0.01) and hatching rates (r = 0.684, p<0.01). Also interaction between egg weights and fertilization rates (r = 0.603, p<0.01) and between hatching and living embryo rates (r = 0.679, p<0.01) were determined significant. On the other hand, egg weights were negatively correlated (r = -0.368, p<0.01) with living embryo rates. For the CPE stimulator, egg weights were significantly correlated with fertilization (r = 0.662, p<0.01), living embryo (r = 0.390, p<0.05) and hatching rates (r = 0.443, p<0.01). Also hatching were significatly correlated with fertilization (r = 0.665, p<0.01) and living embryo rates (r = 0.903, p<0.01). In addition, fertilization and living embryo rates significantly correlated (r = 0.615, p<0.01) with each other.
AtıfWoS3İncele