Sürdürülebilir Kalkınma Amaçları
Yayınlar
- DüzeltmeAçık Erişim2026Scopus SJRQ3Erratum to: Sequence Analysis and Genetic Diversity of ndhF of the Chloroplast DNA (cpDNA) of Chrysophthalmum (Asteraceae) Species in Türkiye
Sevindik, Emre; Gelir, Seda Nur; Demircan, Şelale; Çiftçi, İbrahim; Paksoy, Mehmet Yavuz
The article number was incorrectly given as 30 but should have been 29.
- MakaleYalnız künye2026Scopus SJRQ3Sequence Analysis and Genetic Diversity of ndhF of the Chloroplast DNA (cpDNA) of Chrysophthalmum (Asteraceae) Species in Türkiye
Sevindik, Emre; Gelir, Seda Nur; Demircan, Şelale; Çiftçi, İbrahim; Paksoy, Mehmet Yavuz
Absract: In this study, chloroplast DNA (cpDNA) ndhF sequence analysis was used to determine the sequence analysis and genetic diversity of Chrysophthalmum Schultz Bip. species distributed in Türkiye. Genomic DNA was isolated from green leaves and cpDNA ndhF region was amplified by PCR. The results of the PCR, the forward and reverse sequences were edited using the Sequencher 5.4.6 programme and the contig sequences were obtained. To determine the phylogenetic relationships between species, MEGA 6.0 was used to construct a maximum likelihood (ML) tree. Using the same program, nucleotide composition, phylogenetic trees, genetic distance matrix, and genetic diversity analyses were performed. In addition, the physicochemical properties and 3D structure of ndhF proteins were determined. From the results of the ndhF analyses, the nucleotide length of Chrysophthalmum species was found to vary between 471 and 625. The genetic matrix value was determined to be between 0.000 and 0.009. In the phylogenetic tree constructed using ndhF sequences from Anvillea DC., Pulicaria Gaertn., Limbarda Adans, Inula L., Carpesium L., Pentanema Cass., Dittrichia Greuter, Rhanteriopsis Rauschert and Telekia Baumg species from NCBI, C. montanum was grouped with Inula, Pentanema and Rhanteriopsis species. C. gueneri was grouped with C. dichotomum and Pentanema alanyense. Previous phylogenetic studies were in agreement with our results. In addition, the ndhF results supported that Chrysophthalmum species are not a monophyletic group.
AtıfWoS1
İncele - DüzeltmeYayıncıda açık erişim2026Scopus SJRQ1Correction: Comprehensive Biological and Chemical Evaluation of Two Seseli Species (S. gummiferum and S. transcaucasicum) (Antioxidants, (2021), 10, 10, (1510), 10.3390/antiox10101510)
Zengin, Gokhan; Stojković, Dejan; Mahomoodally, Mohamad Fawzi; Jugreet, Bibi Sharmeen; Paksoy, Mehmet Yavuz; Ivanov, Marija; Gašić, Uroš; Gallo, Monica; Montesano, Domenico
In the original publication [1], there was a mistake in Figure 1 as published. The figure, as published, did not include the appropriate statistical information. We have revised the figure accordingly, and the corrected version of “Figure 1” is presented below. The authors state that the scientific conclusions are unaffected. This correction was approved by the Academic Editor. The original publication has also been updated.
- MakaleYalnız künye2025Scopus SJRQ2Phylogenetic analysis of the nrDNA ITS and cpDNA rbcL sequences of some endemic Dianthus (Caryophyllaceae) species from Türkiye
Sevindik, Emre; Güner, Özal; Yolcu, Seher; Başköse, İsa; Paksoy, Mehmet Yavuz; Khadivi, Ali; Tunç, Yazgan; Yaman, Mehmet
In this study, the phylogenetic relationship of four species of Dianthus genus which are endemic and distributed in Türkiye, including D. zederbaueri Vierch, D. varankii Hamzaoğlu & Koç, D. halisdemirii Hamzaoğlu & Koç, and D. goekayi Kaynak, was determined using nuclear DNA (nrDNA) internal transcribed spacer (ITS) and chloroplast DNA (cpDNA) rbcL regions. After sampling from the plant, DNA was extracted and the ITS (ITS4-ITS5A) and rbcL (rbcLaF-rbcLaR) primers were used to perform PCR amplification. Sequencer 5.4.6. was used to edit the sequences. MEGA 11 software was used to determine the nucleotide ratios, genetic distance matrix, and π = nucleotide diversity. Some species of Dianthus available in the National Center for Biotechnology Information (NCBI) were used to construct maximum likelihood (ML) phylogenetic trees. As a result, based on the ITS analysis π = nucleotide diversity was determined as: 0.001543, and the result of the rbcL analysis π = nucleotide diversity was determined as: 0.000000. In the ITS results, D. halisdemirii, D. superbus, and D. varankii appeared together, while D. zederbaueri and D. aculeatus appeared together. The rbcL results showed that D. zederbaueri and D. varankii were closely related, but the relationship between D. halisdemirii and D. goekayi could not be determined. Furthermore, in the phylogenetic tree constructed by taking the ITS and rbcL sequences of Dianthus, Gypsophila, Silene, Arenaria, Saponaria, Cerastium, Stellaria, Petrorhagia, Minuartia, Agrostemma, and Polycarpon species from NCBI using ITS and rbcL sequences, our endemic Dianthus species appeared in the same group with Petrorhagia, Gypsophila, and Saponaria species. To conclude, our ITS and rbcL results were consistent in determining the phylogenetic relationships of our Dianthus species with other species.
AtıfScopus1İncele - MakaleYalnız künye2025Scopus SJRQ3Phylogenetic Analysis of Lisaea Boiss. (Apiaceae) Species in Türkiye Based on Chloroplast DNA (cpDNA) trnL-intron, trnL-F, matK and rbcL Sequences
Efe, Fadime; Sevindik, Emre; Sevindik, Mustafa; Paksoy, Mehmet Yavuz
Abstract: In this study, Lisaea heterocarpa,Lisaea papyracea and Lisaea strigosa species belonging to the Lisaea genus distributed in Türkiye phylogenetic analysis was determined chloroplast DNA regions. Plant samples were collected from different provinces of Türkiye and taken to the laboratory for genomic DNA isolation, polymerase chain reaction and bioinformatics analysis. As a result of the study, the lowest π-nucleotide diversity was found in the rbcL analyses (0.000917), while the highest π-nucleotide diversity was found in the trnL-F analysis (0.008700). In the phylogenetic trees constructed using trnL intron, trnL-F,and rbcL sequences, the species L. strigosa and L. papyracea appeared together, while L. heterocarpa appeared close to this group. The matK sequences, L. strigosa and L.heterocarpa appeared together, while L. papyracea appeared close to this group. In addition, the phylogenetic trees constructed using sequences from some Apiaceae species from National Center for Biotechnology Information (NCBI) determined that the genus Lisaea is monophyletic. In the trnL-F and rbcL analyses, Lisaea species appeared together with Torilis,Yabea, Daucus, Cuminum, Pternopetalum and Anthriscus species, while in the matK analysis, Lisaea species appeared in a different group from Anthriscus species.
AtıfScopus1WoS1OpenAlex1İncele - MakaleYalnız künye2024Pülümür (Tunceli/Türkiye) Florasına Katkılar
Paksoy, Mehmet Yavuz; Başköse, İsa
Bu çalışma 2018-2022 yılları arasında Tunceli il sınırlarında yer alan Pülümür ilçesi florasına katkı sağlamak amacıyla yapılmıştır. Yapılan bu araştırmada toplanan bitki-lerin teşhis edilmesi sonucunda çalışma sahasında 89 familya ve 433 cinse ait 957 taksonun bulunduğu tespit edilmiştir. Bu taksonlardan 115’i endemiktir ve endemizm oranı %12’ dir. Araştırma sonucunda 304 taksonun Pülümür için yeni kayıt olduğu be-lirlenmiştir. Bitkilerin fitocoğrafik dağılımları sırasıyla; 337’si (%35.2) İran-Turan; 85’i (%8.8) Avrupa-Sibirya; 46’sı (%4.8) Akdeniz; 489’u (%51.2) ise Geniş yayılışlı ve Bilinmeyen olduğu görülmektedir.
- MakaleAçık Erişim2024Scopus SJRQ2Mattiastrum pulumurense (Cynoglossinae, Boraginaceae), a new endemic species from Türkiye
Doğru Koca, Aslı; Gökmen, Mustafa; Yilmaz, Gülderen; Paksoy, Mehmet Yavuz
A new species, Mattiastrum pulumurense, has been described from Tunceli in eastern Türkiye. It is generally distinguished from other species (except M. racemosum var. scabridum) by a sparsely hairy or glabrescent above-ground part. It is most similar morphologically to M. longipes, M. calycinum, and Paracaryum hedgei (P. hedgei is potentially a member of the genus Mattiastrum). However, it differs from M. longipes and M. calycinum by its 3–3.5-mm-long and linear lanceolate scales, 2.5–4.5-mm-long anthers, and 10–15-mm-long styles; it differs from Paracaryum hedgei by its 3–3.5-mm-long scales, 2.5–4.5-mm-long anthers. In addition, this new species differs from M. racemosum var. scabridum by having 3–3.5-mm-long scales and no scale appendages. A description of the new species, an illustration, a comparative Table of morphological characters, detailed photographs, and a distribution map of the new species together with adjacent species were provided. In addition, the evolutionary relationships of these species were calculated using both Bayesian inference and maximum likelihood analyses based on the ribosomal internal transcribed spacer (ITS1-5.8S-ITS2) and combined chloroplast data set (rps16 and trnL genes, trnL-trnF intergenic spacer). According to the phylogeny-based combined chloroplast data set, both populations of Mattiastrum pulumurense generate a monophyletic clade and are closely related to M. longipes, P. hedgei, and M. calycinum. According to ITS-based phylogeny, Mattiastrum pulumurense is closely related to Rindera tetraspis, which is seen in the paraphyletic and nested genera Mattiastrum-Paracaryum-Rindera.
AtıfScopus1
İncele - MakaleAçık Erişim2024Scopus SJRQ1Integrating chemical analysis with in vitro, in silico, and network pharmacology to discover potential functional compounds from Marrubium astracanicum subsp. macrodon
Avşar, Ömer Kaan; Kasbolat, Sevgi; Ak, Gunes; Nilofar; Caprioli, Giovanni; Santanatoglia, Agnese; Uysal, Ahmet; Uba, Abdullahi Ibrahim; Ponniya, Sathish Kumar M.; Paksoy, Mehmet Yavuz
The members of the genus Marrubium are of great interest because they contain biologically active compounds. With in this mind, we aimed to examine the chemical profiles and biological activities (antioxidant, enzyme inhibition and antimicrobial effects) of different extracts of Marrubium astracanicum subsp. macrodon. Extractions of the plants were carried out with the solvent n-hexane, acetone, acetone/water (70 %), and water. The antioxidant activity of these extracts was evaluated through six different assays, namely 2,2-diphenyl-1-picrylhydrazyl (DPPH), 2,2′-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS), cupric reducing antioxidant capacity (CUPRAC), ferric reducing antioxidant power (FRAP), metal chelating ability (MCA), and phosphomolybdenum (PBD). Additionally, the inhibitory effects of these solvents on five enzymes acetylcholinesterase (AChE), butyrylcholinesterase (BChE), tyrosinase, amylase, and glucosidase were investigated. The study detected 38 compounds in a plant, with the highest content in acetone and acetone/water extracts. The acetone/water extract demonstrated the highest antioxidant activity aligning with its superior total phenolic (51.9 mg gallic acid equivalent (GAE)/g) and flavonoid (31.0 mg rutin equivalent (RE)/g) content, highlighting a direct correlation between bioactive compound concentration and antioxidant potential across various assays. Regarding the enzyme inhibition activity, the acetone/water extract of this plant exhibited significant AChE inhibition measuring 2.5 mg galantamine equivalent (GALAE)/g and anti-glucosidase activity measuring 1.2 mmol acarbose equivalent (ACAE)/g. In antimicrobial evaluation, the acetone extract exhibited good antimicrobial activity against B. cereus at 0.2 mg/ml dose. In molecular docking analysis, delphinidin 3,5 diglucoside showed a good interaction with the active site of AChE active. Through the application of network pharmacology, an in-depth exploration was conducted to unravel the potential of compounds from M. astracanicum against Alzheimer's disease. The study specifically focused on identifying key targets within the Alzheimer's disease pathway, pinpointing crucial proteins such as Glycogen synthase kinase-3 (GSK3), Prostaglandin-endoperoxide synthase 2 (PTGS2), and Alzheimer's-associated amyloid precursor protein (APP). In summary, the plant can serve as a valuable ingredient in the production of functional products within the pharmaceutical, nutraceutical, and cosmeceutical applications.
AtıfScopus4OpenAlex4İncele - MakaleAçık Erişim2024Scopus SJRQ1A broad-spectrum biological activities of Heracleum humile extracts: A first report of the antiviral, anti-cancer and chemical properties
Ocal, Mustafa; Duran Yelken, Selda; Altunoglu, Yasemin Celik; Baloglu, Mehmet Cengiz; Soomro, Sanam Iram; Zengin, Gokhan; Angeloni, Simone; Mustafa, Ahmed M.; Caprioli, Giovanni; Paksoy, Mehmet Yavuz
The current work focused on researching the bioactive potential of ethyl acetate (EA), methanol (MeOH), and water extract of H. humile plant, specifically ethyl acetate, methanol extracts were acquired with maceration method, and water extract was obtained by the infusion method. The highest total phenolic ingredient was determined in the water extract, while the highest total flavonoid ingredient was found in the MeOH extract. The water extract displayed the most potent antioxidant activity in the assays used. The ethyl acetate extract showed the highest enzyme inhibition activity in Butyrylcholinesterase inhibition (BChE) and amylase assay. Antibacterial efficiency was analyzed using microdilution analysis. The minimum inhibitory concentration (MIC) amounts ranged from 125 to 500 μg/mL except for water extract. The antiviral potential of the extracts was assessed against Bovine herpes virus 1 (BoHV-1) (Cooper strain). Methanol extract showed more potent antiviral effect. Selectivity indices were calculated for ethyl acetate and methanol extract as 3.32 and 13.39, respectively. All extracts displayed Deoxyribonucleic Acid (DNA) protection activity at approximately 25%–35%. For cell viability evaluation, 3-(4,5-Dimethylthiazol-2-yl)-2,5-Diphenyltetrazolium Bromide (MTT) test was performed against triple-negative human breast adenocarcinoma cells (MDA-MB-231) and Michigan Cancer Foundation-7 (MCF-7) cells. The most effective results were found in ethyl acetate extract at 48h. The 50% inhibitor concentrations (IC50) were determined for MDA-MB-231 and MCF-7 as 116.7 μg/mL and 247.9 μg/mL, respectively. Based on the bioactive compound content results, extracts of H. humile include valuable compounds that possess significant biological properties. In conclusion, these extracts could offer promising novel plant-derived therapeutics.
- MakaleAçık Erişim2024Scopus SJRQ4MOLECULAR MARKERS ANALYSIS OF ENDEMIC BORNMUELLERA HAUSSKN. SPP. (BRASSICACEAE) IN TÜRKİYE; [ANALIZA MOLEKULARNIH MARKERA ENDEMIČNE VRSTE BORNMUELLERA HAUSSKN. SPP. (BRASSICACEAE) U TURSKOJ]
Okan, Kemalcan; Özkara, Zekiye; Sevİndİk, Emre; Sevİndİk, Mustafa; Paksoy, Mehmet Yavuz
Okan K., Z. Özkara, E. Sevindik, M. Sevindik, M. Y. Paksoy (2024). Molecular markers analysis of endemic Bornmuellera hausskn. spp. (Brassıcaceae) ın Türkiye. - Genetika, Vol 56, No.2, 295-304. In this study, molecular characterisation of Türkiye's endemic species Bornmuellera cappadocica (Willd.) Cullen & T.R. Dudley, Bornmuellera glabrescens (Boiss. & Balansa) Cullen & T.R.Dudley, Bornmuellera kiyakii Aytaç & Aksoy and Bornmuellera angustifolia (Hausskn. ex Bornm.) Cullen & T.R.Dudley was carried out using ten RAPD and ten ISSR primers. In RAPD-PCR analysis, 66 bands were obtained and the polymorphism rate was 96.96%. In the ISSR-PCR analysis, 119 bands were obtained and the polymorphism rate was 95.79%. In the UPGMA (Unweighted Pair Group Method with Arithmatic Mean) dendrogram based on RAPD-PCR, B. angustifolia and B. glabrescens were found to be a sister group, and B. kiyakii and B. cappadocica were a sister group. Principal Component Analysis (PCA) analysis based on RAPD-PCR were compatible with the UPGMA dendrogram. In the UPGMA dendrogram based on ISSR-PCR, B. kiyakii and B. glabrescens were found to be sister groups, and B. cappadocica was closely related to this group. PCA analysis based on ISSR-PCR were compatible with the UPGMA dendrogram. As a result, both RAPD and ISSR results a high rate of polymorphism were obtained. The results were compared with previous sequence-based studies, morphological, anatomical and palynological studies.
- MakaleYalnız künye2024Scopus SJRQ2Phylogenetic analysis of the endemic Bornmuellera Hausskn. spp. (Brassicaceae) in Türkiye based on nuclear ITS and chloroplast trnL intron, trnL-F, rbcL and trnQ-rps16 DNA sequences
Okan, Kemalcan; Sevindik, Emre; Paksoy, Mehmet Yavuz
In this study, phylogenetic analysis of Turkish endemic Bornmuellera (Brassicaceae) species was conducted based on nuclear ribosomal DNA (ITS) and chloroplast DNA (trnL intron, trnL-F, rbcL and trnQ-rps16 DNA) sequences. In addition, the relationships between the sequences of some Brassicaceae family species retrieved from NCBI, and Bornmuellera species were documented. All of the plant specimens were collected from different regions of Türkiye, and brought to the laboratory. Total genomic DNA was extracted using the GeneMark kit. After polymerase chain reaction (PCR) analyses, DNA sequences obtained were then edited using Sequencher 5.4, Bioedit 7.2.3 and FinchTV 1.4.0, and analyzed using MEGA 6.0 software. Maximum likelihood (ML) and bootstrap trees were constructed in order to identify the relationships among Bornmuellera species. In the study, the highest nucleotide diversity was detected by rps16-trnQ (0.039146) sequence analysis, while the lowest was detected by rbcL (0.004186) sequence analysis. In addition, according to the phylogenetic analysis results obtained with other Brassicaceae species, it is revealed that the Turkish endemic Bornmuellera genus is monophyletic.
AtıfScopus5WoS4OpenAlex5İncele - MakaleAçık Erişim2024Scopus SJRQ3Heracleum munzurense (Apiaceae), a new species from East Anatolia, Turkey and taxonomic position of Tetrataenium lasiopetalum
Duran, Ahmet; Lyskov, Dmitry; Samigullin, Tahir; Paksoy, Mehmet Yavuz
A new species, Heracleum munzurense (Apiaceae), is described from East Anatolia, Turkey. It grows in damp places formation of the Munzur Mountains in the district of Pülümür (Tunceli Province). The taxonomic affinities of the new species were determined by analyzing morphological data and through a molecular phylogenetic study of nrITS/ETS. The new species is morphologically and genetically related to H. apiifolium and H. pastinaca, but differs mainly in stems, leaves, pedicels, rays and petals pubescence, petals size, fruit hairs, and size features. The anatomical structure of the fruit is given. The diagnostic morphological characters of the new species are discussed. In this study, the geographical distribution of the new species and the related taxa H. apiifolium, H. lasiopetalum, and H. pastinaca is mapped. In addition, the habitat, ecology, phenology, and conservation status of the new species are also provided. Tetrataenium lasiopetalum is also closely related to this group of species. Common morphological features and results of molecular phylogenetic study suggest to transfer this species back to the genus Heracleum.
AtıfScopus1İncele - MakaleYalnız künye2023Scopus SJRQ3MOLECULAR CHARACTERIZATION OF Dittrichia viscosa (L.) GREUTER (ASTERACEAE) POPULATIONS REVEALED BY ISSR MARKERS AND CHLOROPLAST (cpDNA) trnL INTRON SEQUENCES; [MOLEKULARNA KARAKTERIZACIJA POPULACIJA Dittrichia viscosa (L.) GREUTER (ASTERACEAE) POMOĆU ISSR MARKERA I HLOROPLAST (cpDNA) trnL INTRONSKIH SEKVENCI]
Sevindik, Emre; Bozkurt, Makbule; Yilmaz, Mehmet; Şenyüz, Ezginur; Paksoy, Mehmet Yavuz
In this study, molecular characterization of Dittrichia viscosa (L.) Greuter populations collected from Aydın province was carried out based on ISSR technique and chloroplast DNA trnL intron sequences. 10 ISSR primers were used to determine the molecular characterization among the populations. For cpDNA trnL intron amplification, trnC and trnD primers were used. In ISSR analysis, a total of 70 bands were obtained. The polymorphism rate was determined to be approximately 94.28%. According to the ISSR analysis, the UPGMA dendrogram consisted of three groups. For cpDNA trnL intron sequences, phylogenetic analyses were obtained along with genetic distances. For populations, cpDNA trnL intron sequences were determined between 454 and 472 bases. The maximum likelihood phylogenetic tree consist of two clades. In addition, cpDNA trnL intron sequences of some species (Chrysophthalmum Pulicaria Inula Jasonia Stenachaenium Carpesium Blumea Iphiona Limbarda Rhanterium Lifago Duhaldea Chiliadenus) of Asteraceae family from NCBI with Dittrichia viscosa populations phylogenetic tree was constructed. As a result of the study, it was determined that the polymorphism rate of Dittrichia viscosa populations obtained by ISSR-PCR was higher than the results obtained from trnL intron sequences.
AtıfScopus3WoS4İncele - MakaleAçık Erişim2022Tunceli Florası İçin Yeni Kayıtlar
Paksoy, Mehmet Yavuz
Bu çalışma Tunceli ilinin florasına katkı sağlamak amacıyla gerçekleştirilmiştir. Yapılan bu araştırmada toplanan bitkilerin teşhis edilmesi sonucunda 41 familyaya ait 134 cins; bu cinslere ait 177 taksonun (52 alttür ve 9 varyete) Tunceli ili için yeni kayıt olduğu tespit edilmiştir. Taksonlardan 32’si (%18) endemiktir. Floristik analizler sonucu tespit edilen bitkilerin fitocoğrafik dağılımları sırasıyla; 51’i (%28.8) İran-Turan; 19’u (%10.7) Avrupa-Sibirya; 14’ü (%7.9) Akdeniz; 93’ü (%52.6) ise Geniş yayılışlı ve Bilinmeyen olduğu görülmektedir.
AtıfOpenAlex2TR Dizin2İncele - MakaleAçık Erişim2022Genetic Relationship of Seven Endemic Inula L. (Asteraceae) Species Grown in\rTurkey
Paksoy, Mehmet Yavuz; Sevindik, Mustafa; Sevindik, Emre
In this study, genetic relationship of ISSR markers of seven endemic Inula species distributed in\rTurkey was carried out. Plant samples were collected from different regions of Turkey in 2013 and\rgDNA was obtained by DNA isolation from green leaves. Genetic relationship between species was\rdetermined using 12 ISSR primers. PCR products were run on agarose gel electrophoresis and\rvisualized under UV light. All gel images were examined and the presence and absence of\rpolymorphic bands were scored as 0 and 1. A total of 85 bands were obtained from the primers. Of\rthese, 74 polymorphic and 11 monomorphic bands were obtained. The total polymorphism rate was\rfound to be approximately 87.05%. The phylogenetic tree and genetic distances between species\rwere calculated using the PAUP 0 4.0b10 analysis program. According to the distance matrix, the\rgenetic distance was found between the closest Inula helenium subsp. orgyalis and Inula helenium\rsubsp. vanensis (0.29851), while the farthest between Inula sarana and Inula macrocephala\r(0.56000) species. The phylogenetic tree was obtained using the UPGMA algorithm, and the tree\rconsisted of two groups. The results were compared with the morphological, palynological nrDNA\rand cpDNA results of the past. Our findings supported previous studies
AtıfOpenAlex2İncele - MakaleYalnız künye2022Scopus SJRQ2Comparative Content, Biological and Anticancer Activities of Heracleum humile Extracts Obtained by Ultrasound-Assisted Extraction Method
Ocal, Mustafa; Altunoglu, Yasemin Celik; Angeloni, Simone; Mustafa, Ahmed M.; Caprioli, Giovanni; Zengin, Gokhan; Paksoy, Mehmet Yavuz; Baloglu, Mehmet Cengiz
As the safety and effectiveness of synthetic drugs remain in doubt, researchers are trying to develop natural medicines from medicinal plants. Herein, ethyl acetate, methanol and water extracts from the Heracleum humile plant were obtained by an ultrasonic-assisted extraction process and the aim was to evaluate some biological effects of the extracts due to the limited data on the pharmacological properties of Heracleum humile in the literature. Weak antibacterial activity was observed on tested bacterial species. The minimum inhibitory concentration and the minimum bactericidal concentration values ranged from 250 to 500 μg/mL. In addition, cytotoxic activity was determined using the MTT test. The strongest findings were determined for ethyl acetate extract on the MDA-MB-231 cell lines at the 48th hour (IC50:97.94 μg/mL), followed by the MCF-7 cell lines at the 24th hour (IC50:103.9 μg/mL). All extracts of Heracleum humile contained mainly flavonoids, phenolic acids and their derivatives, i. e., well-known compounds that possess numerous biological activities such as antioxidant, anti-inflammatory, anticancer, antimicrobial etc. The study results could provide important information that Heracleum humile could be a potential candidate as a natural enzyme inhibitor. It can be concluded that these extracts could be useful in the elementary step of improving novel plant-derived multifunctional pharmaceuticals.
AtıfScopus6OpenAlex11
İncele - MakaleAçık Erişim2022Scopus SJRQ3Antioxidant, Antityrosinase Activities and Composition of Essential Oils Obtained from Roots and Fruits of Ferulago longistylis Boiss.
Kürkçüoğlu, Mine; Ağalar, Hale Gamze; Temiz, Burak; Duran, Ahmet; Paksoy, Mehmet Yavuz; Başer, Kemal Hüsnü Can
This study was aimed to evaluate the potential uses of Ferulago longistylis Boiss. essential oils in cosmetic applications. The air dried roots and fruits were subjected to water distillation by using a Clevenger apparatus for 3 h. Both essential oils were analyzed by gas chromatography (GC-FID) and gas chromatography-mass spectrometry (GC/MS) systems, simultaneously. The essential oil yields of the fruits and roots were calculated as 4% and 0.91%, respectively. The major compounds were identified as 2,3,6-trimethylbenzaldehyde (29.5%), cis-chrysanthenyl acetate (15.2%), (Z)-β-ocimene (12.4%), α-pinene (12.0%) and myrcene (11.4%) in the fruit essential oil while the root essential oil was characterized with high content of α-pinene (90.5%). Both essential oils were evaluated for their potential antioxidant and antityrosinase activities. The both essential oils showed weak Trolox equivalent antioxidant capacity and DPPH radical scavenging activity. The fruit essential oil showed weak antityrosinase (%25 inhibition, 1 mg/mL) activity while the root essential oil was inactive. The results concluded that both essential oils were not promising in cosmetic applications.
AtıfScopus3OpenAlex1TR Dizin1İncele - MakaleAçık Erişim2022Scopus SJRQ2Prangos munzurensis (Apiaceae), a new species from East Anatolia, Turkey
Lyskov, Dmitry; Duran, Ahmet; Paksoy, Mehmet Yavuz; Samigullin, Tahir
A new species, Prangos munzurensis (Apiaceae), is described and illustrated from Tunceli Province, Eastern Turkey. The new species is related to Prangos heyniae, P. abieticola, and P. denticulata and differs from them in the number of rays, length of bracts, indumentum of petals, ultimate segments of leaves, stylopodium features, fruit morphology and anatomy, and distributional range. It also differs from P. ferulacea in presence of furrows between blocks of inner mesocarp, number of umbel rays, and puberulous petals. A comprehensive description of the morphological and carpo-anatomical features, photographs, and data on the geographical distribution, habitat and ecology, and conservation status are also provided. In addition, P. munzurensis is recognized as a separate species by molecular analysis of nrITS/ETS.
AtıfScopus2OpenAlex2İncele - MakaleYalnız künye2022Scopus SJRQ2An ethno-veterinary study on plants used for the treatment of livestock diseases in Genç (Bingöl-Turkey)
Babacan, Ebru Yüce; Polat, Rıdvan; Güler, Osman; Moyan, Ayşe; Paksoy, Mehmet Yavuz; Çakılcıoğlu, Uğur
There are a very limited number of studies on plants traditionally used in animal diseases in Turkey. As a result, valuable information is getting lost. Traditional plant use in Genç District (Turkey) was recorded by semi-structured interviews with breeders and shepherds. The collected information was subjected to quantitative analysis using the informant consensus factor. It was recorded that a total of 53 plants from 24 families were used in the traditional treatment of animal diseases. 8 of these plants are endemic plants with a narrow distribution area and were recorded for the first time by us. The breeders stated that they applied such plants to animals with peace in mind as these plants had been used for human treatment as well. This study is the first ethnoveterinary study conducted in the province. Phytochemical studies need to be carried out on medicinal plants used in livestock around Bingöl Province. We believe that the use of this preliminary information obtained from ethnoveterinary studies by researchers will contribute to the production of natural animal medicines.
AtıfScopus32İncele - MakaleAçık Erişim2022Scopus SJRQ1Phytochemical Profile and Biological Activities of the Extracts from Two Oenanthe Species (O. aquatica and O. silaifolia)
Świątek, Łukasz; Sieniawska, Elwira; Mahomoodally, Mohamad Fawzi; Sadeer, Nabeelah Bibi; Wojtanowski, Krzysztof Kamil; Rajtar, Barbara; Polz-Dacewicz, Małgorzata; Paksoy, Mehmet Yavuz; Zengin, Gokhan
This study presents the evaluation of biological activities and chemical profiling of Oenanthe aquatica (L.) Poir. and Oenanthe silaifolia M. Bieb. The phytochemical profile, antioxidant, enzyme inhibitory, cytotoxic and antiviral activities of the methanolic and aqueous extracts were investigated. The aqueous extract of O. aquatica possessing the highest content of phenolics (60.85 mg gallic acid equivalent/g extract), also exhibited the strongest radical scavenging potential against 2,2-diphenyl-1-picrylhydrazyl and 2,2′-azino-bis (3-ethylbenzothiazoline-6-sulfonic acid) (79.46 and 148.66 mg Trolox equivalent/g extract, respectively), the highest reducing ability (207.59 and 107.27 mg Trolox equivalent/g extract, for cupric reducing antioxidant capacity and ferric reducing antioxidant activity, respectively), metal chelating potential (33.91 mg ethylenediaminetetraacetic acid equivalent/g extract) and total antioxidant ability (1.60 mmol Trolox equivalent/g extract). Liquid chromatography-electrospray ionization-quadrupole time-of-flight-mass spectrometry (LC-ESI-QTOF-MS/MS) permitted tentative identification of compounds from simple organic acids, phenolic acids, coumarins, flavonoids and their glycosides in O. aquatica and O. silaifolia extracts. The methanolic extract of O. aquatica substantially depressed acetylcholinesterase (3.67 mg galantamine equivalent/g extract), tyrosinase (126.66 mg kojic acid equivalent/g extract), and α-amylase (0.83 mmol acarbose equivalent/g extract) enzymes. The methanolic extract of O. silaifolia showed highest enzymatic inhibitory property against butyrylcholinesterase, and its aqueous extract depressed α-glucosidase activity (0.26 mmol acarbose equivalent/g extract). All tested extracts exerted selective toxicity towards cancer cell lines, and the highest anticancer potential was found for O. aquatica aqueous extract on FaDu and HeLa cells with CC50 of 57.36 and 47.16 µg/mL, respectively. Significant antiviral activity against HSV-1 (HHV-1) was found for both aqueous extracts in concentrations of 1000 µg/mL, which inhibited the HSV-1 cytopathic effect (CPE) in virus infected VERO cells and reduced the virus infective titer by more than 3 log (logCCID50 /mL). This study has produced critical scientific data on O. aquatica and O. silaifolia, which are potential contenders for the development of novel phyto-pharmaceuticals.
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