Sürdürülebilir Kalkınma Amaçları

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Yazarlar

• Eriani, Kartini(2)
• Maulida, Siti(2)
• Wilkes, Martin(2)
• Fadli, Nur(1)
• Handayani, Luvi S.(1)
• Handayani, Luvi Syafrida(1)
• Kocabas, Filiz K.(1)
• Kocabas, Filiz Kutluyer(1)
• Kocabas, Mehmet(1)
• Muchlisin, Zainal A.(1)
• Muchlisin, Zainal Abidin(1)
• Nurlaili, Nurlaili(1)
• Rahayu, Sri R.(1)
• Rahayu, Sri Riska(1)
• Razi, Nanda M.(1)
• Salma, Itsnatani(1)
• Siti-Azizah, Mohd N.(1)
• Siti-Azizah, Mohd Nor(1)

• MakaleYalnız künye2024Scopus SJRQ3/r/munzur/1625
  EFFECT OF CRYOPROTECTANT AND CONCENTRATION ON THE SPERM QUALITY OF WALKING CATFISH, Clarias batrachus, POSTCRYOPRESERVATION

  Handayani, Luvi S.; Maulida, Siti; Rahayu, Sri R.; Razi, Nanda M.; Kocabas, Mehmet; Kocabas, Filiz K.; Wilkes, Martin; Siti-Azizah, Mohd N.; Eriani, Kartini; Fadli, Nur

  BACKGROUND: Walking catfish, Clarias batrachus is one of the native and most popular freshwater catfish species in Indonesia. However, cultivation faces challenges, particularly due to the scarcity of larvae resulting from underdeveloped breeding technologies. Cryopreservation is a method of storing sperm to maintain viability for a long period and support the breeding technology of the fish. Cryoprotectant, in this context, plays an important role in determining the success of sperm cryopreservation. OBJECTIVE: To determine the best type and concentration of cryoprotectant for cryopreservation of walking catfish sperm. MATERIALS AND METHODS: A total of five different types of cryoprotectants, namely DMSO, glycerol, ethyl glycol, ethanol, and methanol, were tested at four concentration levels namely 0%, 5%, 10%, 15%, and 20%, each with four replications. RESULTS: The type and concentration of cryoprotectant had a significant effect on sperm motility and viability (P<0.05). The best outcomes were obtained with 5% DMSO and ethyl glycol, 10% glycerol and methanol, as well as 15% ethanol. CONCLUSION: The highest motility and viability values were obtained with 5% DMSO, resulting in its recommendation for cryopreservation of walking catfish sperm.

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  AtıfScopus5·OpenAlex3

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• MakaleYalnız künye2023Scopus SJRQ3/r/munzur/2196
  EFFECT OF CRYOPROTECTANT AND PRE-FREEZING ON THE SPERM MOTILITY, VIABILITY AND FERTILITY OF GOLDFISH Carassius auratus (PISCES: CYPRINIDAE) POST SHORT-TERM CRYOPRESERVATION

  Nurlaili, Nurlaili; Eriani, Kartini; Salma, Itsnatani; Maulida, Siti; Rahayu, Sri Riska; Handayani, Luvi Syafrida; Kocabas, Filiz Kutluyer; Siti-Azizah, Mohd Nor; Wilkes, Martin; Muchlisin, Zainal Abidin

  BACKGRUND: Goldfish Carassius auratus is a popular ornamental fish extensively cultured worldwide. Sperm cryopreservation is a common fish breeding method that ensures sperm availability around the year. Studies on cryopreservation of goldfish sperm, especially on the suitability of cryoprotectant types and pre-freezing time, are scarcely available. OBJECTIVE: To determine the most suitable type of cryoprotectant and pre-freezing for the successful cryopreservation of goldfish sperm. MATERIALS AND METHODS: A completely randomized design with two factors was utilized in this study. The first factor is the type of cryoprotectants, which included methanol, ethanol, ethylene glycol, glycerol, and DMSO. The second is pre-freezing times of 10, 20, 30, and 40 min at each of the pre-freezing temperatures of 4 0C, -10 0C, and -79 0C, meaning that the total times for the ramping down of temperature were 30, 60, 90 and 120 min, respectively. The Ringer solution and 10% egg yolk were used as extender and extracellular cryoprotectant. The sperm was stored at -179 0C for 7 days. RESULTS: The ANOVA test showed that cryoprotectants and pre-freezing significantly affected the motility, viability, and fertility of goldfish sperm after freezing in liquid nitrogen for 7 days (P<0.05). Furthermore, 10% DMSO combined with 15% egg yolk with an pre-freezing time of 20 min can maintain sperm motility, viability, and fertility higher than other treatments, by 79%, 80%, and 33%, respectively. The agarose gel electrophoresis showed no DNA fragmentation in all samples, including fresh sperm. CONCLUSION: We conclude that 10% DMSO combined with 15% egg yolk and 20 min pre-freezing is the best treatment for goldfish sperm cryopreservation.

  AtıfScopus6·WoS5·OpenAlex5

  İncele
• Dergi/r/munzur/1626
  Cryo-Letters
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