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  • MakaleAçık Erişim2018Scopus SJRQ4
    Effect of glyphosate on some protective systems in Zea mays L; [Glifosatın mısır (Zea mays L.)’da bazı koruyucu sistemlere etkisi]

    Beker Akbulut, Gulcin; Yigit, Emel; Bayram, Dilek

    Glyphosate is an herbicide that is applied after non-selective germination and affects plant growth. In this study, glyphosate was applied to Zea mays L. after germination, at a concentration range from 0.017 to 0.145 M in a growth chamber. The effects of this herbicide on some antioxidant enzymes, lipid peroxidation, total chlorophyll and total carbohydrate content were investigated on days 1, 5 and 10 following the application. Results showed that peroxidase (POD), ascorbate peroxidase (APX), superoxide dismutase (SOD), catalase (CAT) and glutathione-S-transferase (GST) were increased in the groups treated with glyphosate on days 5 and 10, but the activities of reduced glutathione (GSH) and glutathione reductase (GR) were decreased on day 10. Malondialdehyde (MDA) content indicating lipid peroxidation was increased on days 1, 5 and 10. Changes in total chlorophyll and total carbohydrate contents were found to be time-dependent. These increases and decreases in the antioxidant activities, MDA content, total chlorophyll and total carbohydrate content in Zea mays L. were determined as a symptom of cytotoxicity caused due to glyphosate.

    • SDG 2 · Açlığa Son
    AtıfScopus3OpenAlex1
    ScopusTR Dizinİncele
  • MakaleYalnız künye2018Scopus SJRQ1
    Effects of salicylic acid and organic selenium on wheat (Triticum aestivum L.) exposed to fenoxaprop-p-ethyl

    Akbulut, Gulcin Beker; Yigit, Emel; Kaya, Armagan; Aktas, Aydın

    In this study, we evaluated effects of fenoxaprop-p-ethyl herbicide on wheat (Triticum aestivum L.). The wheat plants were grown in pots containing perlite. Fenoxaprop-p-ethyl was applied to adequately grown T. aestivum plants at determined doses (1 mM, 1.5 mM, 2.2 mM, 3.4 mM and 5.1 mM) via spraying. The effects of this herbicide on some antioxidant enzymes, lipid peroxidation, carotenoid, total chlorophyll and total phenolic were investigated on days 1, 5 and 10 following the application. Environmental pollutants cause oxidative stress, production of free radicals and changes in antioxidants. Synthesis of signal molecules such as salicylic acid (SA) is important for the stress response of plants. In addition to this, although selenium is known not to be an obligatory element for plants, low dose selenium has positive effects on the plant growth. We also investigated the effects that are elicited on parameters above mentioned by the treatment of exogenous 0.5 mM SA and 0.004 mM organic selenium (SeI) separately. Our findings reveal that SA and SeI, which are externally applied before the germination of seeds that are exposed to herbicide stress, becomes effective in reduction of the negative effects of fenoxaprop-p-ethyl, with regard to antioxidant system, lipid peroxidation, pigment system and total phenolic. When we evaluate the study results, our findings suggest that especially SA plays a protective role on wheat against herbicide stress.

    • SDG 2 · Açlığa Son
    AtıfScopus21WoS19OpenAlex24
    ScopusWeb of Scienceİncele
  • MakaleYalnız künye2017Scopus SJRQ3
    MONTHLY VARIABILITY OF OXIDANT AND ANTIOXIDANT BIOMARKERS OF FRESHWATER CRAYFISH (ASTACUS LEPTODACTYLUS ESCH., 1823)

    Barim-Oz, Ozden; Yilmaz, Seval; Sahin, Hulya; Benzer, Fulya

    In the present study was determined the effects of monthly variability on enzymatic antioxidant (superoxide dismutase (SOD), catalase (CAT), glutathione peroxidase (GSH-Px)), antioxidant (reduced glutathione (GSH)) and the oxidant status (nitric oxide (NO), lipid peroxidation (MDA)) in hepatopancreas, muscle, gills and gonad tissues of freshwater crayfish (A. leptodactylus) the monthly collected throughout the year from Keban Dam Lake of Turkey. Results indicate that variations in oxidants and antioxidants with relation to month were not only tissue specific but also were gender specific. The levels of oxidant parameters were generally higher in hepatopancreas in comparison to muscle, gills and gonad of crayfish. Moreover, in tissues were also determined the different oxidants and antioxidants responses in moulting, mating, breeding and egg laying periods.

    AtıfWoS5
    Web of Scienceİncele
  • MakaleAçık Erişim2017Scopus SJRQ3
    The effect of harvest time on the bioactive properties and volatile components of lavender (Lavandula officinalis)

    Yalcin, H.; Kavuncuoğlu, H.; Tulukcu, E.; Eroğlu, Z.

    This study was designed to determine the effect of harvest time on the bioactive properties and volatile components of flowers, leaves and stems of Lavandula officinalis. The bioactive properties were examined in terms of total phenolic content, antiradical activity and antioxidant capacity by the Folin-Ciocalteu, 1.1-diphenyl-2-picrylhydrazyl scavenging and phosphomolybdenum methods, respectively. Volatile components were detected with gas chromatography-mass spectrometry. Water and methanol were used for extraction solvent and three different parts of the day (morning, midday and evening) were selected for harvest time. Water extracts had higher total phenolic contents (337.2-771.5 mg gallic acid equivalents (GAE)/g dry extract) than methanolic extracts (170.3-320.8 mg GAE/g dry extract), and in general, midday values were less than morning and evening rates and had a significant difference depending on harvest time. A similar feature was observed in respect to antioxidant properties. The inhibition rates (%) of flowers and leaves were higher than those of the stems for either extract. In terms of the volatile components linalool, camphor, 1,8-cineole, terpinen-4-ol and isoborneol were the most representative compounds in the samples.

    AtıfScopus10WoS10OpenAlex9
    ScopusWeb of Scienceİncele
  • MakaleAçık Erişim2014Scopus SJRQ1
    Cryopreservation of rainbow trout Oncorhynchus mykiss spermatozoa: Effects of extender supplemented with different antioxidants on sperm motility, velocity and fertility

    Kutluyer, Filiz; Kayim, Murathan; Ögretmen, Fatih; Büyükleblebici, Serhat; Tuncer, P. Barbaros

    In present study, it was examined whether addition of different antioxidants to the cryopreservation extenders had an effect on semen post-thaw fertility and motility in rainbow trout (Oncorhynchus mykiss) and also it was investigated the sperm characteristics post-thaw sperm characteristics and fertility. The collected semen was pooled to minimize individual variation. Each pooled ejaculate was split into 12 equal aliquots and diluted with base extenders supplemented with the antioxidants, and a base extender with no additives (control). The pooled semen samples diluted at the ratio of 1:10 by the extenders were subjected to cryopreservation. Antioxidants were separately added to the extenders (one per experimental group): catalase (250 U/l), superoxide dismutase (250 U/l), peroxidase (250 U/l), oxidized glutathione (1.5 mmol/l), reduced glutathione (1.5 mmol/l), L-methionine (1.5 mmol/l), uric acid (0.25 mmol/l), L-ascorbic acid (0.5 mmol/l), alpha-tocopherol (2.0 mmol/l), beta-carotene (0.5 mmol/l) and camitine (0.5 mmol/l). After dilution the semen was aspirated into 0.25 ml straws, the straws were placed on the tray, frozen for 10 min, and plunged into liquid nitrogen. Our results indicated that the post-thaw motility rate increased in extenders supplemented with uric acid, L-methionine, SOD, L-carnitine, alpha-tocopherol and L-reduced glutathione (p < 0.05). The motility duration of frozen thawed semen increased in extenders supplemented with uric acid, L-methionine, SOD, alpha-tocopherol and L-reduced glutathione (p < 0.05). Fertilization rate and hatching rate of frozen-thawed semen was not affected by the tested antioxidants. Consequently, the tested antioxidants affected the motility parameters and cryopreservation extenders could be supplement with antioxidants. This study suggested usage of antioxidants in the cryopreservation of rainbow trout. (C) 2014 Elsevier Inc. All rights reserved.

    AtıfWoS76OpenAlex92
    Web of SciencePubMedİncele
  • MakaleAçık Erişim2012Scopus SJRQ3
    The Effects of Different Dosage of Kefir with Different Durations on Growth Performances and Antioxidant System in the Blood and Liver Tissues of Coruh Trout (Salmo coruhensis)

    Can, Erkan; Kurtoglu, Ilker Zeki; Benzer, Fulya; Erisir, Mine; Kocabas, Mehmet; Kizak, Volkan; Kayim, Murathan; Çelik, Huseyin Tugrul

    The objective of the present work is to examine the effects of different doses (0, 10, 20 and 40 g kg(-1) fish diet mass) of kefir on growth performance and oxidant-antioxidant status in the blood and liver tissues of Coruh trout, Salmo coruhensis, in different periods (2 and 3 months). In this study, survival was more than 88.3%, and irrespective of dietary kefir levels at the end of the study. There were no significant differences in SGR (Specific growth rate), FCR (Feed conservation rate), CF (Condition factor) among fish fed diets with 0, 10, 20 and 40 g kg(-1) kefir, however, these growth values were significantly different in terms of durations between 2-month and 3-month treatment (P<0.05). Our results indicated that TAS (Total Antioxidant Status) and TOS (Total Oxidant Status) in blood tissues reduced with kefir treatment at the end of third month (P<0.05). Malondialdehyde (MDA) levels in liver decreased in all groups compared with control group and a considerable extent decrease was observed in 40 g dose of kefir treatment at the end of third month. The data obtained from this experiment indicated that the same dose of kefir was more effective at the end of 3-month treatment than 2-month treatment (P<0.001). Although there was no statistical difference among groups, an increase in the glutathione peroxidase (GSH-Px) enzyme activity was observed in all groups compared to control groups. While catalase (CAT) activity decreased in all groups compared to control group at the end of second month (P<0.01), the decrease was insignificant level at the end of third month. In glutathione (GSH) levels, statistical differences were no observed in all groups compared to the control group with 2-month treatment while there were significant increases with 3-month treatment (P<0.001). It was concluded that kefir could play an antioxidant role and its effectiveness depended on dosage and time of application in Coruh trout, S. coruhensis.

    • SDG 2 · Açlığa Son
    AtıfWoS15OpenAlex15
    Web of Scienceİncele