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- MakaleAçık Erişim2023Scopus SJRQ2Fabrication of hydroxyapatite-based nano-gold and nano-silver-doped bioceramic bone grafts: Enhanced mechanostructure, cell viability, and nuclear abnormality properties
Demirel, M.; Aslan, N.; Aksakal, B.; Arslan, M.E.
In this study, nano-gold (nAu) and nano-silver (nAg) were doped at the molar ratios of Molar5–Molar30 to the Hydroxyapatite (HAp)-based bioceramic bone graft synthesized by the sol–gel method. The effects of nAu and nAg on structural, mechanical, cell viability, and nuclear abnormality of the synthesized bioceramic grafts were evaluated. The chemical and morphological properties of the bone grafts after production were examined through XRD and SEM–EDX analyses and mechanical tests. To determine the biocompatibility of the bone grafts, cell viability tests were performed using human fibroblast cells. In the cytotoxicity analyses, only HAp and HAp-nAu5 grafts did not show toxicological properties at any concentration, while HAp-nAg5 among the nAg-containing grafts gave the best results at the 200–100 μg/mL concentrations and showed significant cytotoxicity in human fibroblast cells. The other nAu-containing grafts showed toxicological properties in the concentration range of 200–50 μg/mL and nAg-containing grafts in the concentration range of 200–100 μg/mL against the negative control. The micronucleus (MN) analyses showed that the lowest total MN and L (lobbed) amounts, while the lowest total N (notched) amount, was obtained from the only HAp graft. It was found that the nAg-doped bone grafts gave higher total MN, L, and N amounts compared to the nAu-doped bone grafts. Furthermore, while the mean nuclear abnormality (NA) values of all grafts gave close results, the highest values were again obtained from the nAg-doped bone grafts.
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İncele - MakaleYalnız künye2017Scopus SJRQ2The effect and characterization of newly synthesized SrBr2 reinforced bone grafts on structure and cell viability
Demirel, Mehtap; Aksakal, Bünyamin; Kaya, Ali İhsan
Abstract: In this study, a highly durable SrBr2 compound was added to Ca(NO3)2·4H2O, KOH, NaNO3, P2O5, and urea (CO(NH2)2) compounds at different ratios (wt 10–30%); biografts were synthesized in different compositions using the sol gel method They were characterized using X-ray diffraction, Fourier transform infrared spectrometer, scanning electron microscope, and mechanical tests e.g. hardness and compression. Structural, mechanical properties and cell viabilities of the synthesized biografts were comparatively examined. Fourier transform infrared spectrometer and X-ray diffraction analysis results showed that, including SrBr2, hydroxyapatite had formed in the biografts. Also, hardness and compression tests revealed that compressive stress and hardness values of the grafts increased as the amount of SrBr2 increased. The cell viability ratios also enhanced with the amount of SrBr2 increased in the synthesized biografts. Graphical Abstract: [InlineMediaObject not available: see fulltext.]
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