Sürdürülebilir Kalkınma Amaçları
Yazarlar
- Onder Erguven, Gokhan(3)
- Serdar, Osman(3)
- Cikcikoglu Yildirim, Nuran(2)
- Demirci, Ulas(2)
- Durmus, Barbaros(2)
- Erguven, Gokhan Onder(2)
- Yildirim, Numan(2)
- Arslan Topal, E. Is̨ıl(1)
- Bayhan, H.(1)
- Bayhan, Hurrem(1)
- Duygu Tekin(1)
- Erguven, G. O.(1)
- Ikizoglu, B.(1)
- Ince, Muharrem(1)
- Kanat, G.(1)
- Kardas, Elif(1)
- Ketenalp, Zozan(1)
- Nuhoglu, Y.(1)
- Tanyol, Mehtap(1)
- Tatar, Şule(1)
- Topal, Murat(1)
- Yildirim, Nuran Cikcikoglu(1)
- Yusuf Söylemez(1)
- Yıldırım, Numan(1)
- Öbek, Erdal(1)
- Önder Erguven, Gokhan(1)
Yayınlar
- MakaleAçık Erişim2024Scopus SJRQ3sThe Bioremediation of Industrial Effluents by Phanerochaete Chrysosporium: The COD, TOC Removal Efficiency and Biochemical Assessment for Dreissena Polymorpha
Serdar, Osman; Yıldırım, Numan; Onder Erguven, Gokhan; Ketenalp, Zozan; Cikcikoglu Yildirim, Nuran; Durmus, Barbaros
Bioremediation is one of the cheapest and easy method for biological treatment for most kind of industial wastewater. Bioremediation potential of the Phanerochaete chrysosporium for industrial wastewater from Industrial Zone Wastewater plant in Mardin, Turkey, was evaluated. The chemical oxygen demand (COD) and total organic carbon (TOC) reduction efficiencies of P. chrysoporium of on wastewater studied using biomarkers such as catalase (CAT), superoxide dismutase (SOD), glutathione peroxidase (GPx), Thiobarbituric Acid (TBARS) and reduced glutathione (GSH) levels in Dreissena polymorpha. The level of TBARS, a marker of lipid peroxidation was found to be high because of wastewater exposure after treatment. TBARS levels decreased (from 20.2 to 31.6 and from 20.8 to 37.5). GSH levels (from 52.3 to 15.9), CAT levels (from, 119.7 to 15.3 and from 91.1 to 13.4) SOD levels (from 3.8 to 0.9 and from 3.4 to 1.2) and GPX activity (from 177.2 to 104.4 and 174.2 to 100.5) decreased in the wastewater exposure groups during 24 and 96th hour compared to the control groups. GSH levels, CAT, SOD and GPX activity increased after treatment by P. chrysosporium. Results indicate that industrial wastewater caused oxidative stress in Dreissena polymorpha. The findings revealed that the highest removal efficiency for COD and TOC, at 86.3 % and 80.3 % respectively, occurred in the 1/20 diluted medium. P. chrysosporium has proven to be effective in the bioremediation of wastewater from the Industrial Zone Changes in biochemical parameters before and after bioremediation showed that antioxidant parameters such as CAT, SOD, GPx activities and GSH and TBARS levels can be used as biomarker to evaluate bioremediation efficiency.
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İncele - Yüksek Lisans TeziAçık Erişim2023Toprak ortamında herbisit biyoremediasyon veriminin Gammarus pulex'in bazı biyokimyasal paramatreler kullanarak araştırılması
Yusuf Söylemez
Bu çalışmada glifosat herbisitinin tarım alanlarında kullanılan konsantrasyonu baz alınarak hazırlanan sulu çözeltileri, laboratuvarda oluşturulmuş beş adet yapay tarla düzeneklerine ilave edilmiş ve bu düzeneklerin her birine çeşitli konsantrasyonlarda B. aryabhattai bakterisi ilave edilmiştir. Oluşturulan bu düzeneklerden günden güne süzüntü alınarak kimyasal oksijen ihtiyacı (KOİ) ve toplam organik karbon (TOK) parametrelerine bakılıp B. aryabhattai bakterisinin gösterdiği giderim verimleri takip edilmiştir. Arıtım öncesi ve en yüksek giderim veriminin olduğu arıtım sonrası alınan süzüntü suyunun G. pulex üzerinde gösterdiği toksik etkiler de süperoksit dismutaz (SOD), katalaz (CAT), asetilkolinesteraz (AChE), glutatyon (GSH) gibi enzimatik ve enzimatik olmayan antioksidan parametrelerle, lipid peroksidasyonun göstergesi olan tiyobarbitürik asit reaktif maddesi (TBARS) seviyelerindeki değişimlerle takip edilmiştir. Çalışmadan elde edilen verilere göre B. aryabhattai bakterisi en yüksek giderim verimini 10 ml' lik B. aryabhattai ortamında KOİ bazında % 71.1; TOK bazında ise % 85.6 seviyesinde tespit edilmiştir. Enzim aktivite sonuçlarına bakıldığında ise MDA düzeyleri ile AChe kontrol ile kıyaslandığında üç farkli subletal dozda glifosat uygulanan gruplarda hem 24. hem de 96. saatin sonunda arttığı, GSH düzeyleri ile SOD, CAT enzim aktivitesi azaldığı tespit edilmiş ve dolayısıyla B. aryabhattai' nin biyoremediasyon faaliyetlerinde etkin bir bakteri türü olduğunun yanı sıra G. pulex'in de alıcı ortamlarda toksik yanıt araştırmalarında uygun bir model canlı olduğu görüşüne varılmıştır.
- Yüksek Lisans TeziAçık Erişim2022Pyroxasulfone herbisitinin bazı toprak bakterileriyle biyoremediasyonunun karşılaştırılması ve Daphnia magna üzerindeki mortalite etkisi
Duygu Tekin
Bu çalışma ile tarım toprağından izole edilen M. arthrosphorea, M. radiotolerans ve bunların karışımlarıyla elde edilen zenginleştirilmiş mikrobiyal kültürlerle Pyroxasulfone etken maddeli herbisitin kimyasal oksijen ihtiyacı (KOİ) ve toplam organik karbon (TOK) gibi önemli bazı çevresel parametreler bazında elde edilen giderim verimleri araştırılmıştır. Çalışmada bunlara ek olarak giderim öncesi ve sonrası Daphnia magna (su piresi) üzerindeki mortalite (ölüm oranı) etkisi de belirlenmiştir. Kullanılan bakteriler zenginleştirilmiş ve gerekli hacimlerde alınarak Pyroxasulfone etken maddeli herbisitin tarım alanlarında kullanımı tavsiye edilen konsantrasyonları (375, 500, 750 ve 1000 ppm) esas alınarak hazırlanan çözeltilere ilave edilmiştir. Elde edilen en yüksek giderim oranlarındaki numuneler ve tarım arazilerinde kullanımı tavsiye edilen konsantrasyonlarda hazırlanan numuneler ile Daphnia magna üzerindeki ölüm oranları belirlenmiştir. Elde edilen verilere göre KOİ ve TOK giderim yüzdeleri her iki bakteri türü ve bunların karışımlarında çeşitli zaman dilimlerinde %37 - 90 aralığında belirlenmiştir. Arıtılmamış ortamlarda 96 saat sonunda Daphnia Magna üzerinde %40 - 80 civarında, arıtılmış ortamlarda ise %75 – 100 aralığında değişen bir mortalite etkisi tespit edilmiştir. Çalışma sonuçlarına göre M. arthrosphorea, M. radiotolerans ve bunların konsorsiyumlarının pyroxasulfone etken maddeli herbisitler üzerinde tarlaya atılması tavsiye edilen konsantrasyonlarda etkili bir biyoremediasyon gerçekleştirdiği, ancak elde edilen en yüksek giderime sahip nihai KOİ ve TOK konsantrasyonlarndaki ortamların da Daphnia magna üzerinde toksik etki yarattığı gözlemlenmiştir. Bu durum da pyroxasulfone herbisitinin parçalanma ürünlerinin gösterdiği toksik etki ile açıklanmaktadır. Anahtar kelimeler: Pyroxasulfone, Biyoremediasyon, Daphnia magna, Biyokimyasal oksijen ihtiyacı, Kimyasal Oksijen İhtiyacı, Toplam organik karbon
- MakaleAçık Erişim2022Scopus SJRQ2The Bioremediation Capacity of Sphingomonas melonis for Methomyl-Contaminated Soil Media: RSM Optimization and Biochemical Assessment by Dreissena polymorpha
Önder Erguven, Gokhan; Serdar, Osman; Tanyol, Mehtap; Cikcikoglu Yildirim, Nuran; Yildirim, Numan; Durmus, Barbaros
The bioremediation capacity of S. melonis for methomyl investigated with RSM. Oxidative stress and neurotoxic response determined in D. polymorpha. COD and TOC levels were measured. GSH, MDA levels and CAT, SOD, AChE activities in D. polymorpha exposed to before (group A) and after (group B) bioremediated media during 24 and 96 h were tested by using ELISA kit. AchE activity was decreased 24th h but increased during 96th h in group B compared with group A (p<0.05). MDA levels and CAT activities were decreased during 24 h and 96 h in group B compared with group A (p<0.05). GSH levels were increased during 24 h and 96 h in group B compared with group A (p<0.05). SOD activity did not show a significant change in group B (p<0.05). Methomyl could be effectively remediated by S. melonis and CAT, AChE activities and GSH, TBARS levels of D. polymorpha are useful biomarkers for evaluating the bioremediation capacity.
AtıfScopus5WoS6OpenAlex6İncele - MakaleAçık Erişim2022Scopus SJRQ3The use of Phanerochaete chrysosporium as an alternative bioremedia-tion tool of heavy metal, chemical oxygen demand and phosphate from landfill leachate
Yildirim, Numan; Kardas, Elif; Ince, Muharrem
In this study, in order to investigate the bioremediation efficiency of Phanerochaete chrysosporium, chemical oxygen demand (COD) reduction, total phosphate levels and heavy metal (Fe2+, Mn2+, Zn2+ and Cu2+) removal were determined in five different experimental groups (control, X, Y, Z, and T) under static culture conditions containing leachate at different incubation periods (5, 10, and 20 d). At the end of the 20th day of incubation, 84% Mn2+ and Zn2+ removal was achieved in X application group. In the same application group, 90.88% COD reduction was also obtained. The lowest total phosphate value was determined as 2.63 g L–1 in T group compared with control group (5.0 g L–1). Similar improvements have been identified in other application groups in terms of metal removal, total phosphate levels and COD reduction. As a result, the findings obtained from our study show that white rot fungus P. chrysosporium can be used as an alternative biological resource in the studies for improving landfill leachate in terms of metal removal efficacy, COD and total phosphate level reduction capacity.
AtıfScopus3WoS1OpenAlex3İncele - MakaleYalnız künye2021Scopus SJRQ1Evaluation of the efficiency of chlorpyrifos-ethyl remediation by Methylobacterium radiotolerans and Microbacterium arthrosphaerae using response of some biochemical biomarkers
Onder Erguven, Gokhan; Tatar, Şule; Serdar, Osman; Yildirim, Nuran Cikcikoglu
This study reveals out detoxifying and antioxidant enzyme response of Gammarus pulex exposed/polluted to chlorpyrifos-ethyl insecticide before and after biodegradation/bioremediation by Methylobacterium radiotolerans and Microbacterium arthrosphaerae. Cytochrome P450 1A1, glutathione S-transferase, catalase, and superoxide dismutase activities in G. pulex exposed to chlorpyrifos-ethyl before and after bioremediation/biodegradation by these two bacteria during 24 and 96 h tested by using commercial ELISA kits. The activity of catalase enzyme was decreased depending on chlorpyrifos-ethyl before and after bioremediation/biodegradation the enzyme activity was increased repeatedly. Superoxide dismutase activity level increased after chlorpyrifos-ethyl exposure in 96 h (p > 0.05). Following bioremediation, superoxide dismutase enzyme activity decreased again during 24 h (p > 0.05) and increased during 96 h (p < 0.05). Statistical differences were not found in cytochrome P450 1A1 enzyme activity before and after the process (p > 0.05). No significant differences were determined during the activity of glutathione S-transferase in 24 h (p > 0.05). The activities of glutathione S-transferase were increased after exposure of chlorpyrifos-ethyl during 96 h. After bioremediation; the activity of glutathione S-transferase increased even more (p < 0.05). The results determined that activities of G. pulex at superoxide dismutase, catalase, and glutathione S-transferase are common biomarkers for revealing out the efficiency of bioremediation of chlorpyrifos-ethyl with these two types of soil bacteria. [Figure not available: see fulltext.]
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İncele - MakaleYalnız künye2021Scopus SJRQ1Using Ochrobactrum thiophenivorans and Sphingomonas melonis for bioremediation of Imidacloprid
Erguven, Gokhan Onder; Demirci, Ulas
To solve the environmental problems of the pesticides, bioremediation methods are commonly useful for their environment friendly properties. These methods are more cheap, useful and have more advantages for remediate receiving environments In this study, imidacloprid which is one of the selective insecticide in all over the world is chosenfor bioremediation studies with two soil bacteria. The imidacloprid used in the Mediterranean climate for cotton, pistachios, tomatoes, peppers and eggplant cultivation. The bioremediation of this insecticide was investigated via the performance of O.thiophenivorans and S. melonis in agitated culture media. The agricultural soil used for the isolation of these two bacteria was taken from the cotton cultivated agricultural area in Adana province in Turkey. For this purpose, the isolated and enriched bacteria were added to the recommended insecticide concentrations, and the Imidacloprid active material, Chemical Oxygen Demand (COD), Biological Oxygen Demand (BOD5), Total Organic Carbon (TOC), pH and dissolved oxygen (DO) parameters were determined for two weeks on each day of the study. O. thiophenivorens and S. melonis bioremediate the Imidacloprid insecticide with high efficiency (over 90%). According to these results, it is understood that these bacteria can be preferred in the bioremediation of receiving environments that are contaminated with such pesticides and can be useful for scientists to handle the opposite effects of such persistent organic pollutants and COD, BOD5 and TOC parameters can easily give opinions about decreasing of pesticides like the active materials
AtıfScopus28WoS27OpenAlex30İncele - MakaleAçık Erişim2020Scopus SJRQ1Remediation of pollutants with economical importance from mining waters: Usage of Cladophora fracta
Topal, Murat; Arslan Topal, E. Is̨ıl; Öbek, Erdal
The accumulations of elements (Mo, Cu, Zn and Ni) those have both pollutant characteristics and economic importance were determined in the macroalga (green alga Cladophora fracta) exposed to mining water with the aim of presentation of the possibility of using the alga as a bioindicator of these elements. The highest element concentrations (Mo, Cu, Zn and Ni) accumulated by Cladophora fracta were determined as 2.38; 19.66; 4510.5 and 19.4 mg/kg, respectively. Bioconcentration factor values decreased in the order: Ni>Cu>Mo>Zn. The Metal Pollution Index (MPI) values calculated between 17.50 and 44.98 were used to determine the pollution degree of Cladophora fracta. As a result, it was determined that Cladophora fracta highly accumulated the elements from the mine gallery water. Therefore, Cladophora fracta was a good bioaccumulator and indicator for the remediation of the elements in mine gallery waters.
AtıfScopus8WoS4OpenAlex8İncele - MakaleAçık Erişim2020Scopus SJRQ2Statistical evaluation of the bioremediation performance of Ochrobactrum thiophenivorans and Sphingomonas melonis bacteria on Imidacloprid insecticide in artificial agricultural field
Onder Erguven, Gokhan; Demirci, Ulas
Background: Pesticides are applied directly on the soil or on the vegetation, and thus, they can reach the receiving environment easily. In this way, environmental damage that stems from pesticides also affects public health and the natural habitat. Pesticides are one of the most harmful pollutant groups in terms of human health, fauna and the environment. They penetrate the application field and the applicator right after the application and start to show adverse effects. Methods: The bioremediation of the Imidacloprid (C9H10ClN5O2) insecticide, which is used commonly in Mediterranean climate, was compared with some soil bacteria in artificially prepared fields. For this purpose, firstly, it was determined whether the soil samples taken from a field where cotton was cultivated in Adana in Turkey was suitable for bioremediation. Then, the bacteria were isolated from these soils with the 16sRNA method. The enhanced microbial consortia of these isolated bacteria were inoculated to the artificial fields, meanwhile, the recommended concentrations of Imidacloprid were added to these agricultural fields. Imidacloprid, Chemical Oxygen Demand (COD), Biochemical Oxygen Demand (BOD5) and Total Organic Carbon (TOC) measurements were performed every day for two weeks on the filtrate samples taken from the artificial fields. Results: As a result of the monitoring, it was determined that Ochrobactrum thiophenivorans (Ot) and Sphingomonas melonis (Sm) species and their mixtures could eliminate the Imidacloprid pesticide within two weeks’ time. The removal efficiencies were 100% for active ingredient for each bacterium and their mixtures while COD were 97% and 96% for Ot. and Sm., respectively. TOC and BOD5 removal rates were 97% for both types and their mixtures in one or two-week period. Mixture of Ot and Sm shows 98.5% for COD, BOD5 parameters and 97.5% for TOC parameter. Conclusions: The results that will be obtained will help in the rehabilitation of the receiving environments that are exposed to pesticides in our country and take precautions to avoid the accumulation of pesticides in the body of the humans who are at the top of the food chain.
AtıfScopus23İncele - MakaleYalnız künye2016Scopus SJRQ3ROLE OF SOME ISOLATED SOIL CULTURES ON REDUCTION OF HERBICIDE TRIFLURALIN
Erguven, Gokhan Onder; Bayhan, Hurrem
The study is about the removal of herbicide Trifluralin as COD parameter and monitoring the population dynamic of isolated cultures. These cultures were isolated from soil samples taken from these areas for their identifications before the use of herbicide. Removal of Trifluralin was determined by microbiological degradation with these produced bacteria and fungi in liquid media. With these growing bacteria and fungi, removal rate by bioremediation method studies were carried out. In addition to biodegradation mechanism, with the measurements of the turbidity; the colony number of microorganisms is monitored with isolated bacteria and fungi species. The removal efficiency for Bacillus simplex was 95% and Clostridium tetani species was 86%, by the fungi Penicillium simplicissimum, and Metacordcyceps chlamydosporia species, were 59% and 80% respectively in five days. Bioremediation study results have shown differences with respect to species in liquid media. Degradation time of these herbicides in liquid culture media with isolated cultures varied between 5 min. and 135 min.
AtıfWoS1İncele - MakaleYalnız künye2016Scopus SJRQ3The capacity of some newly bacteria and fungi for biodegradation of herbicide trifluralin under agiated culture media
Erguven, G. O.; Bayhan, H.; Ikizoglu, B.; Kanat, G.; Nuhoglu, Y.
Bioremediation is the use of microorganisms to degrade environmental contaminants (pesticides, polyaromatic hydrocarbons etc.) into less toxic forms or compounds. In this study microbial biodegradation of trifluralin was performed in liquid media with 11 different types of identified fungi and bacteria cultures and their mixtures in agiated culture media. The isolated fungi and bacteria mixtures showed the highest degradation, reaching 93% in the chemical oxygen demand (COD) parameter in four days and 82% as trifluralin active ingredient in five days. Bacteria and fungi mixtures achieved 69% and 66% degradations of trifluralin active ingredient respectively. In the fungi studies, the best removal was achieved by M. Chlamydosporia at 80%, in the bacteria studies, the best removal was achieved by Bacillus simplex about 95% in five days. These different removal rates were due to the microbial differencies.
AtıfWoS16OpenAlex2İncele