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- MakaleAçık Erişim2020Scopus SJRQ3Genetic variation and molecular relationships taxa of conringia heist. EX FABR. (BRASSICACEAE) Based on rapd markers in Turkey
Sevgndgk, Emre; Paksoy, Mehmet Yavuz; Aydoğan, Melike; Topseçer, Feyzanur
In this study, genetic variation and phylogenetic analysis of 13 populations of 6 species belonging to Conringia genus spreading in Turkey were performed using RAPD markers. Genomic DNA isolation from the leaves of the Conringia plant samples was performed via using a commercial kit. Seven RAPD primers were used to identify the genetic diversity between the populations. Polymerase Chain Reaction (PCR) was performed using DNA samples and primers. PCR products were resolved using agarose gel electrophoresis and visualized under UV light. All gel images were analyzed, and the absence and presence of polymorphic bands were scored. The total of 34 DNA bands were detected by seven RAPD primers. PAUP 4.0b10 analysis program was used to calculate phylogenetic tree and genetic distances between the species. The phylogenetic tree was obtained using the UPGMA algorithm and it was composed of two clades. According to the PAUP analysis, the species having the closest distance between each other are C. planisiliqua (Ankara-AyaG) and C. planisiliqua (Ankara-Nallihan) with the value of 0.000 and those having the longest distance are C. grandiflora (Akseki Cukurkoy) and C. orientalis (Elazig-Baskil) with the value of 0.6000. The results suggest that the RAPD markers are useful tools to demonstrate the genetic relationships between populations of the Conringia species.
AtıfScopus6WoS3OpenAlex6
İncele - MakaleYalnız künye2019Scopus SJRQ4MICROMORPHOLOGICAL AND ANATOMICAL INVESTIGATIONS ON CONRINGIA HEIST. EX FABR.
Selvi, Selami; Aladi, Hatice Inci; Paksoy, Mehmet Yavuz
The micromorphological and anatomical investigations on 6 taxa of Conringia Heist. ex Fabr. growing in Turkey were carried out. The anatomy of root, stem and leaves was examined in detail. In micromorphological studies stem and leaf surface of species was examined. Mesophyll type of lamina is dorsiventral (Conringia planisiliqua, C. persica, C. austriaca) and equifacial (C. clavata, C.grandiflora, C. orientalis). Stomata situated on both surfaces, are anisocytic and rarely anomocytic types. Crystals of calcium oxalate observed in only one species (C. grandiflora) was found to have cubic type parenchyma cells of the stem and leaf In micromorphological studies the surface of the stem and leaves were found to be glabrous. Epidermal surface of leaves was observed to be covered with waxy cuticular particles. Anticlinal walls of the adaxial cells are undulate and rarely straight and anticlinal walls of abaxial epidermis are undulate and rarely sinuate. Periclinal walls were found to be covered with upper waxy layer which was flat. A diagnostic key based on combined stem, leaf and leaf epidermal characteristics are presented. The leaf mesophyll structures, stem vascular bundles number, lignified or unlignified of sclerenchyma, epidermal surface and absence or presence of crystals were found to be important characters for the identification of Conringia species.
AtıfWoS1İncele