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- MakaleAçık Erişim2023Scopus SJRQ4Single and combined effects of dimethoate and malathion on oxidative stress biomarkers in the non-target freshwater mussel Dreissena polymorpha
Serdar, Osman; Yildirim, Numan; Yildirim, Nuran Cikcikoglu
This study evaluates the single and combined effects of dimethoate (DI) and malathion (MA) on oxidative stress biomarkers in the freshwater mussel Dreissena polymorpha, estimating the potential harm of these pesticides on aquatic ecosystems. Superoxide dismutase (SOD) and catalase (CAT) activities, and glutathione (GSH) and lipid peroxidation (TBARS) levels were determined in D. polymorpha exposed to sublethal concentrations of DI, MA and a combination of the two during 24 and 96 h periods. The results showed that the GSH levels were decreased but the TBARS levels were increased in all the exposure groups after 24 and 96 h compared to the control. It was observed that SOD activity decreased but CAT activity increased in all the DI exposure groups after 24 h compared to the control. At the end of 96 hours, it was observed that CAT and SOD activities increased again in some exposure groups compared to the control. Exposure time also had an effect on biomarkers in different levels. According to the results, the cytotoxicity of DI and MA combination depended on their concentrations. DI or/ and MA, in sub-lethal concentrations, induced oxidative damages in D. polymorpha. Combined exposure of the pesticides can alter their toxicity and may be evidence of increased toxicity and oxidative stress.
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İncele - Doktora TeziAçık Erişim2022Probiyotiklerin zebra midye (Dreissena polymorpha)'de malathion toksisitesi üzerindeki iyileştirici etkisinin bazı biyokimyasal parametreler ile belirlenmesi
Neşe Akçanal Ödün
Probiyotikler, konakçı olduğu organizmaların sağlığı açısından yararlı sindirim kanalı mikroorganizmaları olarak tanımlanabilirler. Artan nüfusun gıda gereksinimlerini karşılamak amacıyla birim alandan daha fazla ürün elde etmek için zararlılarla mücadelede kullanılan pestisitler, sentetik veya doğal ürünlerdir. Pestisit kullanımının ekosistem üzerinde doğrudan ve dolaylı birtakım zararları bulunmaktadır. Sunulan bu çalışmada, malathion etken maddeli pestisitin hedef dışı sucul bir organizma olan zebra midye (Dreissena polymorpha) üzerindeki etkisinin biyobelirteçlerle belirlenmesi, probiyotik kullanımı esnasındaki koruyucu etkisinin biyobelirteçlerle belirlenmesi amaçlanmıştır. Malathiona maruz bırakılan D. polymorpha'da LC50 değeri 120,11 ± 19,32 mg/l olarak hesaplanmıştır. Probiyotik ile muamele edilen D. polymorpha bireyleri ve doğrudan D. polymorpha bireyleri malathion etken maddeli pestisite LC50 değerine oranla (1/5, 1/10 ve 1/20) sublethal konsantrasyonlarına 24 ve 96 saatlik zaman zarfında maruziyet sağlanmıştır. Deneme gruplarından alınan doku supernatantlarında, tiobarbitürik asit reaktif maddeleri (TBARS) ve redükteglutatyon (GSH) düzeyleri ile superoksitdismutaz (SOD), katalaz (CAT) ve glutatyon peroksidaz (GPx), enzim aktiviteleri tespit edilmiştir. Ayrıca deneme gruplarından alınan örneklerde histopatolojik yanıt değerlendirilmiştir. Malathiona maruz bırakılan D. polymorpha bireylerinde TBARS seviyelerinde artışa, GSH seviyesinde azalışa, SOD enzim aktivesinde azalışa, CAT enzim aktivesinde azalışa, GPx enzim aktivitesinde azalışa sebebiyet verdiği tespit edilmiştir. Histopatolojik yanıtın araştırıldığı gruplarda artan konsantrasyona bağlı olarak uygulama gruplarında probiyotik kullanılmayan gruba kıyaslandığında probiyotik kullanılmayan gruplarda şiddetli lezyonlar tespit edilmiştir Koruyucu olarak uygulanan probiyotik ile muamele edilen grupların hepsinde doğrudan maruziyete kıyasla istatistiksel açıdan anlamlı farklar bulunmuştur. Probiyotik ile muamele edilen grupların histopatolojik yanıtları doğrudan uygulama gruplarına kıyasla hafif ve orta şiddetli lezyonlar olarak koruyucu etkisi gösterdiği belirlenmiştir. Yapılan deneysel çalışmada D. polymorpha'da malathion uygulamasında kullanılan biyobelirteçlerin faydalı olduğu biyokimyasal ve histopatolojik analizlerle belirlenmiştir. Sucul organizmalarda probiyotik kullanımının iyileştirici etkisinin olduğu hem biyokimyasal hem de histopatolojik biyobelirteçlerle teyit edilmiştir.
- MakaleYalnız künye2020Scopus SJRQ4Examining the biochemical effect of malathion pesticide on gammarus pulex (l., 1798)
Yukse, Fahrettin; Aydin, Rahmi; Serdar, Osman; Pala, Avsegul
This study determined the effect of a pesticide with malathion as active substance on Gammams pulex in terms of oxidative stress and antioxidant status. Firstly, 96-hours LC50 value of malathion on G. pulex was determined. Then, the organisms were exposed to determined sublethal concentrations (at the rate of 1/40, 1/20 and 1/10 LC50 value) of malathion for 24 and 96 hours. Thiobarbituric Acid Rcactive Substances (TBARS), rcduccd glutathione (GSH) levels, superoxide dismutase (SOD), catalase (CAT) and glutathione peroxidase (GPx) enzyme activities in the control group and samples that were exposed to malathion for 24 and 96 hours were determined. The results showed that TBARS level significantly increased in G. pulex that was exposed to malathion for 24 and 96 hours compared to the control group (p <0.05). However, GSH level, SOD, CAT and GPx activities dccrcascd (p <0.05). Malathion exposure caused oxidative stress in G. pulex. These results revealed that MDA, GSH levels, SOD, CAT and CiPx activities can be used as effective biomarkcrs in G. pulex.
AtıfScopus1İncele - MakaleAçık Erişim2018Scopus SJRQ3The chemical and biochemical oxygen demand reduction by armillaria tabescens in malathion supplemented culture medium
Yildirim, N.; Erguven, G.O.; Adar, E.
The extensive use of pesticides for controlling insects has been widely used in agricultural activities. However, the indiscriminate use of the pesticides has inflicted serious harmful problems to humans in the ecosystem. Our study aims to evaluating the capacity of Armillaria tabescens to remove the chemical oxygen demand (COD) and biochemical oxygen demand (BOD5) at different concentration of malathion insecticide (50, 100 and 150 ppm) under agitated (130 rpm) submerged culture conditions at 27 °C after 8 and 15 days. According to our results, A. tabescens has achieved COD reduction as 52.39, 27.24, and 38.30% at concentration of 50, 100 and 150 ppm respectively on 15 days. The BOD5 reduction rates were found as 78.2, 74.76 and 81.26% at 50, 100 and 150 ppm concentration respectively at the end of the 15th days. At the end of this time period, the dried biomass of A. tabescens was weighted and we have suggested that malathion in medium reduced the biomass production compared the control group (Sabouraud dextrose broth + A. tabescens). Our experiments have focused that; A. tabescens could be an alternative and useful fungus for bioremediation of wastewater containing malathion insecticide.
AtıfScopus3WoS3OpenAlex4İncele - MakaleAçık Erişim2018Scopus SJRQ1Malathion-induced spermatozoal oxidative damage and alterations in sperm quality of endangered trout Salmo coruhensis
Kocabaş, Mehmet; Kutluyer, Filiz; Benzer, Fulya; Erişir, Mine
The use of pesticides has been increased along with increasing the farming activities and has caused environmental impacts deleteriously. In particular, non-target organisms including fish can be affected by toxic effects of pesticides. Therefore, the impacts of malathion (MTN) on oxidative stress and sperm quality were investigated in vitro. The MTN concentrations used on this study were 0 (control), 75, 100, and 125 μg/L. Lipid peroxidation (MDA), non-enzymatic (GSH), and enzymatic (SOD, GSH-Px, and CAT) activities in spermatozoa were examined for determination of oxidative stress status. Our findings showed that motility rate and period of sperm cells significantly decreased with exposure to MTN. Biochemical assays revealed that CAT activity and levels of MDA, GSH increased in spermatozoa based on concentration while activity of GSH-Px and SOD decreased. Consequently, spermatozoa were highly sensitive to MTN exposure. MTN has disruptive effects on sperm quality and caused to oxidative stress in spermatozoa.
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İncele - MakaleAçık Erişim2017Scopus SJRQ2The ability of Phanerochaete chrysosporium (ME446) on chemical oxygen demand remediation in submerged culture medium supplemented with malathion insecticide
Erguven, Gokhan Onder; Yildirim, Numan; Adar, Elanur
This present study aims to evaluating the efficiency of P. chrysosporium (P.c) to degrade the different concentrations of malathion (MLT) (50, 100 and 150 ppm) under agitated (130 rpm) submerged culture conditions via reduction of chemical oxygen demand (COD). We have previously screening the growth of fungus in sabouraud dextrose agar (SDA) medium for 6 days. We have also determined the laccase activity during degradation process for 15 days. According to our results the fungus has achieved 99.6, 98.8 and 98.7 % COD reduction at concentration of 50, 100 and 150 ppm respectively the end of the 15 days. The highest laccase activity (180 ± 5.69 U/L) was measured at concentration of 50 ppm the end of the 15 days and the lowest activity (36 ± 3.26) was determined at concentration of 150 ppm the end of the 15 days. The dried biomass of fungus was weighted at end of the 15 days and we have determined that malathion in culture medium reduced the biomass production compared the control group (SDB + P.c). Our results showed that, P. chrysosporium could be an effective bioremediation tool for treatment of malathion containing wastewater.
AtıfScopus11WoS11OpenAlex13İncele