Sürdürülebilir Kalkınma Amaçları
Yayınlar
- MakaleAçık Erişim2019Isparta’da Yetişen Bazı Elma Çeşitlerinin Meyve Eti, Kabuk ve Çekirdek Yuvasındaki Antioksidan Kapasitesinin Belirlenmesi
Çimen, Murat; Benzer, Fulya; Yıldırım, Mahsum; Barış, Dilan; Yıldırım, Hüseyin; Karaca Sanyürek, Nesrin; Karakavuk, Emrah
Bu çalışmada Isparta ili Eğirdir ilçesinden temin edilen beş farklı elma çeşidinde meyve eti, kabuk ve çekirdek yuvasındaki antioksidan kapasiteleri ABTS, DPPH ve TFM yöntemleri kullanılarak belirlenmiştir. Analiz sonuçları incelendiğinde çeşitler, alınan kısımlar ve yöntemler bakımından antioksidan seviyelerinin farklılık gösterdiği görülmüştür. En yüksek antioksidan aktivite ‘Granny Smith’ çeşidinde bulunmuştur. En düşük antioksidan aktivite ise ‘Pink Lady’ çeşidinde tespit edilmiştir. Meyve eti, kabuk ve çekirdek yuvasındaki antioksidan miktarları sırasıyla %29.93, %21.95, %27.22 olarak tespit edilirken, TFM metodu ile yapılan analizlerde antioksidan miktarı ABTS ve DPPH metotlarına göre daha yüksek bulunmuştur.
AtıfOpenAlex6TR Dizin4
İncele - MakaleYalnız künye2019Scopus SJRQ3Palliative effect of curcumin on doxorubicin-induced testicular damage in male rats
Aksu, Emrah Hicazi; Kandemir, Fatih Mehmet; Yıldırım, Serkan; Küçükler, Sefa; Dörtbudak, Muhammed Baheeddin; Çağlayan, Cüneyt; Benzer, Fulya
This study aimed to investigate the effect of curcumin (CUR) on doxorubicin (DOX)-induced testicular damage in male rats. Thirty-five adult male Wistar rats were used. Control group was received saline for 7 days. CUR group received CUR for 7 days. DOX group received single dose DOX on the 5th day. DOX+ CUR-100 group received 100 mg/kg/day CUR for 7 days and DOX injection on the 5th day. DOX + CUR-200 group received 200 mg/kg/day CUR for 7 days and DOX injection on the 5th day. DOX treatment decreased in sperm motility rate, live sperm percentages, cellular antioxidants, and increased malondialdehyde (MDA) levels, necrosis, degenerations, and slimming in seminiferous tubules, and DNA damages in testes by inducing oxidative stress. CUR treatment mitigated significantly these side effects when compared with DOX group in a dose-dependent manner. In conclusion, CUR treatment can be used in the mitigation of DOX-induced testicular toxicity.
AtıfScopus56WoS51OpenAlex68
İncele - MakaleAçık Erişim2019Scopus SJRQ3Effect of the organophosphate insecticide chlorpyrifos exposure on oxidative stress and quality of Salmo coruhensis spermatozoa
Kutluyer, Filiz; Kocabaş, Mehmet; Erişir, Mine; Benzer, Fulya
The use of pesticides has been increasing along with increasing farming activities and has caused deleterious environmental impacts. Non-target organisms in particular, including fish, are affected by pesticides. In this work, the impacts of Chlorpyrifos (CPF) on sperm oxidative stress markers and sperm motility were investigated in vitro. CPF concentrations were 0 μg/L (control), 5 μg/L, 10 μg/L and 15 μg/L. Lipid peroxidation [malondialdehyde (MDA)], nonenzymatic antioxidants [glutathione (GSH)] and enzymatic [superoxide dismutase (SOD), glutathione peroxidase (GSH-Px) and catalase (CAT)] activities in sperm cells were examined for the determination of oxidative stress status. Our findings showed that motility and survival of sperm cells significantly decreased with exposure to Chlorpyrifos. Biochemical assays revealed that CAT activity and levels of MDA and, GSH increased in spermatozoa based on CPF concentration while activity of GSH-Px and SOD decreased. Consequently, spermatozoa were highly sensitive to CPF exposure. It can be deduced that CPF has the potential to disrupt sperm quality and to cause to oxidative stress in sperm cells of S. coruhensis.
AtıfScopus15WoS14OpenAlex13İncele - MakaleAçık Erişim2019Scopus SJRQ2l-Arginine Improves Sperm Motility in Threatened Coruh Trout Salmo coruhensis (Teleostei: Salmonidae)
Kocabaş, Mehmet; Kutluyer, Filiz; Erişir, Mine; Benzer, Fulya
l-arginine is a versatile amino acid and precursor of nitric oxide that provides protection for preventing free radical damage. Herein, experiments were designed to clarify the effect of l-arginine supplementation on the sperm motility of a threatened trout, Coruh trout Salmo coruhensis. Activation solution was supplemented with levels of 0 mM (Control), 2 mM, 4 mM, and 8 mM l-arginine, and motility and survival of sperm cells were assessed. The selected concentrations were determined according to results of our preliminary experiments. The sperm motility rates at l-arginine concentrations of 0, 2, 4, and 8 mM were 73.33 ± 5.77%, 76.67 ± 5.75%, 80.01 ± 0.01%, and 90.00 ± 0.05%, respectively; notility durations were 21.67 ± 2.08, 32.33 ± 3.79, 32.67 ± 1.15, and 37.00 ± 6.38 s, respectively. Significant effect of l-arginine supplementation was determined on the percentage and duration of motile spermatozoa (p < 0.05). The maximum increment was evoked at the greatest concentration (8 mM). Here, we showed that l-arginine supplement can improve sperm motility of S. coruhensis.
AtıfScopus1WoS1OpenAlex2İncele - MakaleAçık Erişim2018Scopus SJRQ4The Effect of Naringenin on Oxidative Stress in Some Tissues (Heart, Lung, Brain, Spleen, Muscle) of Lead-treated Rats
Benzer, Fulya; Dağ, Üzeyir; Erişir, Mine; Özkaya, Ahmet
Naringenin, a flavonoid increases resistance against oxidative stress. The present study aims to investigate the effect of lead on lipid peroxidasyonu and some antioxidants in heart, lung, brain, spleen, muscle and the effect of naringenin on these parameters. Naringenin was administered by orogastric gavage (50 mg/kg, dissolved in corn oil) and lead acetate was given as daily 500 parts per million in drinking water for 4 weeks. In the rats exposed to lead, MDA levels in the brain, lungs, spleen and muscle tissues significantly increased (P<0.001), in the heart tended to increase but not significantly (P>0.05). Lead caused a statistically significant decrease in GSH-Px activity of the brain, spleen and muscle tissues, in CAT activity of the heart, lung and muscle tissues, in GSH concentration of the lungs and muscle tissues (P<0.05). The administration of alone naringenin caused a statistically significant decrease in MDA levels in the heart and muscle, but a significant increase in the lung (P<0.05). Although increased MDA levels in the heart, lungs, brain, muscle and spleen tissues were significantly decreased by use of the naringenin together with lead (P<0.05), increased MDA levels in the lungs and spleen tissues could not be recovered to the normal level. Generally the decreased GSH-Px and CAT activities in these tissues due to lead were significanly increased by naringenin supplementation (P<0.05). In addition, the reduced GSH levels in lung and muscle, the inceased GSH levels in heart with effect of lead were returned to the normal by naringenin. Lead particularly causes to an increase in lipid peroxidation by consuming antioxidants of cells. Naringenin was able to prevent as tissue-specific (brain, muscle) the oxidative stress caused by lead.
AtıfOpenAlex2TR Dizin2İncele - MakaleYalnız künye2018Scopus SJRQ3Effects of conventional and organic rearing systems and hen age on oxidative stress parameters of blood and ovarian tissues in laying hens
Benzer, Fulya; Baykalır, Yasin; Erişir, Mine; Şimşek, Ülkü Gülcihan
Bu çalışmada yetiştirme sistemleri ve tavuk yaşının, tavukların serum ve yumurtalık dokusundaki bazı oksidatif stres parametreleri üzerine etkileri incelendi. Bu amaçla 30 ve 60 haftalık yaşta, iki farklı sistemden (konvansiyonel kafes ve organik) toplam 48 adet ticari yumurtacı hibrit olan Bovans White kullanıldı. Kesim işlemini takiben tavukların serum ve yumurtalık dokuları toplanarak, malondialdehid (MDA), glutatyon (GSH), glutatyon peroksidaz (GSH-Px) ve katalaz (CAT) düzeyleri belirlendi. Konvansiyonel kafeste yetiştirilen hem 30, hem de 60 haftalık tavukların serum MDA düzeyleri önemli derecede yüksek bulundu (P<0.05). Konvansiyonel kafeste GSH-Px aktivitesi düşük, aksine CAT aktivitesi önemli düzeyde yüksek tespit edildi (P<0.05). Tavuk yaşı serum MDA, GSH, GSH-Px ve CAT düzeylerini etkilemedi (P>0.05). Buna ek olarak, serumda, yetiştirme sistemi ve tavuk yaşı arasında önemli bir etkileşim bulunmadı (P>0.05). Konvansiyonel sistemdeki tavukların yumurtalık dokusunda MDA (P=0.010) ve GSH (P<0.01) seviyeleri önemli düzeyde yüksek bulunurken, GSH seviyesi 60 haftalık yaşta arttı (P<0.01). GSH-Px ve CAT aktiviteleri gruplar arasında benzer çıktı (P>0.05). Yetiştirme sistemi ve tavuk yaşı arasındaki etkileşim yumurtalık dokusunda CAT aktivitesini etkiledi (P<0.05). Sonuç olarak, konvansiyonel bataryalı kafesler, lipid peroksidasyon ve MDA düzeylerinin artmasıyla ilişkili olarak tavuklarda oksidatif strese neden oldu. Bu sistemde MDA'nın azalmasında rol oynayan antioksidan enzimler yetersiz kaldı. Oksidan/antioksidan dengesi 30 ve 60 haftalık yaşlarda tavuk organizması tarafından kurulabildi ve dolayısıyla dokuların MDA düzeyleri yaştan etkilenmedi
AtıfTR Dizin2İncele - MakaleAçık Erişim2018Scopus SJRQ3Cypermethrin-induced in vitro alterations on oxidative stress and quality of salmo coruhensis spermatozoa
Kutluyer, Filiz; Kocabaş, Mehmet; Erişir, Mine; Benzer, Fulya
The use of insecticides has been increasing along with increasing agriculture activities and has caused deleterious environmental impacts. Non-target organisms in particular, including fish, are affected by pesticides. In this work, the impacts of cypermethrin (CYP) on sperm oxidative stress markers and sperm motility were investigated in vitro. The CYP concentrations were 0 μg L-1 control, ethanol), 1.025 μg L-1, 2.05 μg L-1 and 4.1 μg L-1. Lipid peroxidation [Malondialdehyde (MDA)], non-enzymatic antioxidants [glutathione (GSH)] and enzymatic [superoxide dismutase (SOD), glutathione peroxidase (GSH-Px) and catalase (CAT)] activities in sperm cells were examined for determination of oxidative stress status. Our findings showed that motility and survival of sperm cells significantly decreased with exposure to CYP. Biochemical assays revealed that CAT activity and levels of MDA and, GSH increased in spermatozoa based on CYP concentration while activity of GSH-Px and SOD decreased. Consequently, spermatozoa were highly sensitive to CYP exposure. It can be deduced that CYP has the potential to disrupt sperm quality and to cause to oxidative stress in sperm cells of S. coruhensis.
AtıfScopus3WoS3OpenAlex3İncele - MakaleAçık Erişim2018Scopus SJRQ1Malathion-induced spermatozoal oxidative damage and alterations in sperm quality of endangered trout Salmo coruhensis
Kocabaş, Mehmet; Kutluyer, Filiz; Benzer, Fulya; Erişir, Mine
The use of pesticides has been increased along with increasing the farming activities and has caused environmental impacts deleteriously. In particular, non-target organisms including fish can be affected by toxic effects of pesticides. Therefore, the impacts of malathion (MTN) on oxidative stress and sperm quality were investigated in vitro. The MTN concentrations used on this study were 0 (control), 75, 100, and 125 μg/L. Lipid peroxidation (MDA), non-enzymatic (GSH), and enzymatic (SOD, GSH-Px, and CAT) activities in spermatozoa were examined for determination of oxidative stress status. Our findings showed that motility rate and period of sperm cells significantly decreased with exposure to MTN. Biochemical assays revealed that CAT activity and levels of MDA, GSH increased in spermatozoa based on concentration while activity of GSH-Px and SOD decreased. Consequently, spermatozoa were highly sensitive to MTN exposure. MTN has disruptive effects on sperm quality and caused to oxidative stress in spermatozoa.
AtıfScopus14WoS14OpenAlex13İncele - MakaleYalnız künye2018Scopus SJRQ2Chemoprotective effects of curcumin on doxorubicin-induced nephrotoxicity in wistar rats: by modulating inflammatory cytokines, apoptosis, oxidative stress and oxidative DNA damage
Benzer, Fulya; Kandemir, Fatih Mehmet; Kucukler, Sefa; Comaklı, Selim; Caglayan, Cuneyt
Doxorubicin (DXR) is one of the most important chemotherapeutic agent. However, nephrotoxicity reduces its clinical utility in humans. The aim of the study was to investigate protective effects of curcumin (CMN) against DXR-induced nephrotoxicity. Rats were subjected to oral treatment of CMN (100 and 200 mg/kg body weight) for 7 days. Nephrotoxicity was induced by single intra peritoneal injection of DXR (40 mg/kg body weight) on the fifth day and then the experiment was terminated on the eighth day. Nephroprotective effects of CMN were associated with decrease in serum toxicity markers and increase in antioxidant enzyme activities. CMN was able to reduced the levels of inflammatory markers such as TNF-α, NF-κB, IL-1β, iNOS and COX-2 in the rats. It also reduced the expressions of apoptotic marker including caspase-3, and oxidative DNA damage marker including 8-OHdG. Collectively, these findings indicated that CMN protect against DXR-induced nephrotoxicity.
AtıfScopus116WoS111OpenAlex122İncele - MakaleAçık Erişim2018Scopus SJRQ4Comparison of oxidant and antioxidant status of Çoruh trout (Salmo coruhensis), anatolian trout (Salmo rizeensis) and rainbow trout (Oncorynchus mykiss) spermatozoa
Kutluyer, Filiz; Kocabaş, Mehmet; Eri̇Şi̇r, Mine; Benzer, Fulya
The aim of present study was to compare oxidant and antioxidant status of Çoruh trout (Salmo coruhensis), Anatolian trout (Salmo rizeensis) and rainbow trout (Oncorynchus mykiss) spermatozoa. Fish were obtained from Uzungöl. Enzymatic antioxidant activities (superoxide dismutase, catalase, glutathione peroxidase), glutathione and lipid peroxidation (malondialdehyde) were determined in spermatozoa of three trout species. Results indicated that catalase (23.36±0.36 K/g.protein), glutathione peroxidase (74.00±1.5 U/g.protein), glutathione (0.57±1.24 µmol/g.cell) and malondialdehyde levels (6.55±2.01 nmol/g cell) were highest levels in Anatolian trout (S. rizeensis) spermatozoa. In conclusion, differences among species caused alterations in the antioxidant and malondialdehyde levels.
AtıfScopus1OpenAlex2TR Dizin1İncele - MakaleAçık Erişim2018Scopus SJRQ3Effects of conventional and organic rearing systems and hen age on oxidative stress parameters of blood and ovarian tissues in laying hens; [Konvansiyonel ve organik yetiştirme sistemleri ile tavuk yaşının yumurta tavuklarının kan ve yumurtalık dokusundaki oksidatif stres parametreleri üzerine etkisi]
Şimşek, Ülkü Gülcihan; Baykalir, Yasin; Erişir, Mine; Benzer, Fulya
In this study, effects of hen age and rearing systems on some oxidative stress parameters in serum and ovarian tissues of hens are investigated. For this purpose, total of 48 Bovans White commercial laying hybrids from two different rearing systems (conventional cage and organic) at 30 and 60 weeks of age were used. Serum and ovarian tissues of the hens were collected following slaughter process and malondialdehyde (MDA), glutathione (GSH), glutathione peroxidase (GSH-Px) and catalase (CAT) levels of serum and ovarian tissues were determined. MDA levels of serum was found significantly higher in conventional system, both in 30 and 60 weeks old (P<0.05). GSH-Px activity was lower, conversely CAT activity was significantly higher in conventional system (P<0.05). Hen age didn’t affect MDA, GSH, GSH-Px and CAT levels of serum (P>0.05). In addition, there weren’t any significant interactions between rearing system and hen age in serum (P>0.05). MDA (P=0.010) and GSH (P<0.01) levels of ovarian tissue were found significantly higher in conventional system while GSH levels of ovarian tissue had increased in 60 weeks old (P<0.01). GSH-Px and CAT activity was similar between groups (P>0.05). Interaction between rearing system and hen age affected activity of CAT in ovarian tissue (P<0.05). As a result, conventional battery cages caused oxidative stress interrelated with increasing lipid peroxidation and MDA levels in laying hens. Antioxidant enzymes that play role in reduction of MDA were inadequate in this system. Oxidant/antioxidant balance can be established at 30 or 60 weeks of age by hen organism and therefore MDA levels of tissues would not be affected by age.
AtıfScopus5WoS5OpenAlex5İncele - MakaleYalnız künye2018Scopus SJRQ3Curcumin ameliorates doxorubicin-induced cardiotoxicity by abrogation of inflammation, apoptosis, oxidative DNA damage, and protein oxidation in rats
Benzer, Fulya; Kandemir, Fatih Mehmet; Ozkaraca, Mustafa; Kucukler, Sefa; Caglayan, Cuneyt
Doxorubicin (DXR) is a highly effective drug for chemotherapy. However, cardiotoxicity reduces its clinical utility in humans. The present study aimed to assess the ameliorative effect of curcumin against DXR-induced cardiotoxicity in rats. Rats were subjected to oral treatment of curcumin (100 and 200 mg/kg body weight) for 7 days. Cardiotoxicity was induced by single intraperitoneal injection of DXR (40 mg/kg body weight) on the 5th day and the rats sacrificed on 8th day. Curcumin ameliorated DXR-induced lipid peroxidation, glutathione depletion, decrease in antioxidant (superoxide dismutase, catalase, and glutathione peroxidase) enzyme activities, and cardiac toxicity markers (CK-MB, LDH, and cTn-I). Curcumin also attenuated activities of Caspase-3, cyclooxygenase-2, inducible nitric oxide synthase, and levels of nuclear factor kappa-B, tumor necrosis factor-α, and interleukin-1β, and cardiac tissue damages that were induced by DXR. Moreover, curcumin decreased the expression of 8-OHdG and 3,3′-dityrosine. This study demonstrated that curcumin has a multi-cardioprotective effect due to its antioxidant, anti-inflammatory, and antiapoptotic properties.
AtıfScopus154WoS143OpenAlex163İncele - MakaleYalnız künye2017Ornithobacterium rhinotracheale ile Enfekte Edilen Broylerlerde Lipid Peroksidasyon ve Bazı Antioksidanlar Üzerine Enrofloksasin ve Kafeik Asit Fenetil Ester'in Etkisi *
Benzer, Fulya; Taşdemir, Bülent; Temizer Ozan, Sema; Kılıç, Ayşe; Erişir, Mine; Simsek, Halil; Güler, Osman
Bu araştırmada 4 haftalık 96 broyler civciv 8 gruba bölündü. I. Kontrol Grubu, II. Antibiyotik Grubu, III. Kafeik asit fenetil ester (KAFE) Grubu, IV. Antibiyotik+KAFE Grubu, V.Ornithobacterium rhinotracheale (ORT) Grubu, VI. ORT+Antibiyotik Grubu, VII. ORT+KAFE Grubu ve VIII. ORT+Antibiyotik+KAFE Grubu. ORT 3263/91 suşunu (serotip A) 3.8x108 CFU/mL içeren inokulum aerosol olarak verildi (Grup V-VIII). Gruplara antibiyotik ve KAFE uygulanmasına ORT verilmesinden sonraki 17. günde aynı zamanda başlatıldı. 6 gün süre ile enrofloksasin (10 mg/kg/gün) içme sularına katıldı (Grup II, IV, VI, VIII), KAFE (10 µmol/kg) i.p olarak uygulandı (Grup III, IV, VII, VIII). ORT enfeksiyonu sonucu akciğer dokusunda malondialdehit (MDA) düzeyi (P<0.01) ve superoksid dismutaz (SOD) aktivitesi (P<0.05), trake dokusunda MDA (P<0.001) ve glutatyon (GSH) (P<0.05) düzeyi ile glutatyon peroksidaz (GSH-Px) (P<0.01) aktivitesi arttı, akciğerde GSH-Px (P<0.001) ve katalaz (KAT) aktiviteleri ise (P<0.05) azaldı. Akciğerde kontrol grubuna antibiyotik ve KAFE tek başına ve birlikte uygulanması MDA düzeyinde istatistiki olarak anlamlı artışa (P<0.05), GSH-Px (P<0.01) ve KAT (P<0.001) aktivitelerinde önemli azalmaya sebep oldu. ORT enfeksiyonu sonucu akciğer dokusunda meydana gelen MDA seviyesindeki artış üzerine antibiyotik ve KAFE tek başına ve birlikte önemli azaltıcı etki gösterdi (P<0.001). Trekada kontrol grubuna tek başına antibiyotik ve Ant+KAFE uygulanması sonucu MDA düzeyinde istatistiki olarak anlamlı artış (P<0.001) saptandı. Enfeksiyon grubunda Ant ve KAFE'nin tek başına ve birlikte verilmesinin artan MDA üzerine önemli bir etkisi bulunmadı. Enrofloksasin ve KAFE enfeksiyon durumunda akciğer oksidatif hasarını önlerken treka için etkili bulunmadı. Enrofloksasin ve KAFE kanatlılarda bakterial akciğer hastalıklarında oksidatif hasarı azaltmak için önerilebilir.
- MakaleYalnız künye2017Scopus SJRQ3MONTHLY VARIABILITY OF OXIDANT AND ANTIOXIDANT BIOMARKERS OF FRESHWATER CRAYFISH (ASTACUS LEPTODACTYLUS ESCH., 1823)
Barim-Oz, Ozden; Yilmaz, Seval; Sahin, Hulya; Benzer, Fulya
In the present study was determined the effects of monthly variability on enzymatic antioxidant (superoxide dismutase (SOD), catalase (CAT), glutathione peroxidase (GSH-Px)), antioxidant (reduced glutathione (GSH)) and the oxidant status (nitric oxide (NO), lipid peroxidation (MDA)) in hepatopancreas, muscle, gills and gonad tissues of freshwater crayfish (A. leptodactylus) the monthly collected throughout the year from Keban Dam Lake of Turkey. Results indicate that variations in oxidants and antioxidants with relation to month were not only tissue specific but also were gender specific. The levels of oxidant parameters were generally higher in hepatopancreas in comparison to muscle, gills and gonad of crayfish. Moreover, in tissues were also determined the different oxidants and antioxidants responses in moulting, mating, breeding and egg laying periods.
AtıfWoS5İncele - MakaleYalnız künye2016Scopus SJRQ3-
Yıldırım, Nuran Cıkcıkoğlu; Özçelik, Mehtap; Benzer, Fulya
The effects of dietary antioxidants (vitamine E, vitamine C, Astaxhantin, ? carotene) on arginase activity and nitric oxide levels of hepatopancreas and muscle of freshwater crayfish (Actacus leptodactylus) were investigated during moulting.150 mg kg-1 vitamine E (VE), 200 mg kg-1vitamine C (VC), 200 mg kg-1 Astaxhantin and 200 mg kg-1 ? carotene were added to experimental diets. A. leptodactylus with these diets were fed with 2% of their total weight daily, in a day during 75 days. Arginase activity was increased in muscle of crayfish fed the diets VC, VE, astaxanthin and ? karoten but in hepatopancreas arginase activity was increased with ? carotene when compared to control. NO levels in muscle were decreased when compared to control but this decrease was not statically significant. NO levels in hepatopancreas of crayfish fed the diets VC and astaxanthin were higher than control groups. Our results demonstrated that dietary antioxidants affect the arginase activity and nitric oxide level of freshwater crayfish (Astacus leptodactylus, Esch. 1823) during moulting.
AtıfTR Dizin1İncele - MakaleAçık Erişim2016Scopus SJRQ3The Effects of Dietary Antioxidants on the Arginase Activity and Nitric Oxide Level of Narrow-Clawed Turkish Crayfish (Astacus leptodactylus, Esch 1823) in Moulting Period
Benzer, Fulya; Özçelik, Mehtap; Yildirim, Nuran Cikcikoglu
The effects of dietary antioxidants (vitamine E, vitamine C, Astaxhantin, beta carotene) on arginase activity and nitric oxide levels of hepatopancreas and muscle of freshwater crayfish (Actacus leptodactylus) were investigated during moulting. 150 mg kg(-1) vitamine E (VE), 200 mg kg(-1) vitamine C (VC), 200 mg kg(-1) Astaxhantin and 200 mg kg(-1) beta carotene were added to experimental diets. A. leptodactylus with these diets were fed with 2% of their total weight daily, in a day during 75 days. Arginase activity was increased in muscle of crayfish fed the diets VC, VE, astaxanthin and beta karoten but in hepatopancreas arginase activity was increased with beta carotene when compared to control. NO levels in muscle were decreased when compared to control but this decrease was not statically significant. NO levels in hepatopancreas of crayfish fed the diets VC and astaxanthin were higher than control groups. Our results demonstrated that dietary antioxidants affect the arginase activity and nitric oxide level of freshwater crayfish (Astacus leptodactylus, Esch. 1823) during moulting.
AtıfWoS3OpenAlex3İncele - MakaleAçık Erişim2016Scopus SJRQ1The in vitro effect of cypermethrin on quality and oxidative stress indices of rainbow trout Oncorhynchus mykiss spermatozoa
Kutluyer, Filiz; Benzer, Fulya; Erisir, Mine; Ögretmen, Fatih; Inanan, Burak Evren
There is limited information on the scientific literature about the effect of in vitro exposure of fish sperm to pesticides. In vitro effect of cypermethrin on sperm quality and oxidative stress has not yet been fully investigated. Therefore, the effects of cypermethrin, a type II pyrethroid insecticide, on quality and oxidative stress of spermatozoa were examined in vitro. To explore the potential in vitro toxicity of cypermethrin, fish spermatozoa were incubated with different concentrations of cypermethrin (1.025, 2.05 and 4.1 mu g/l) for 2 h. The motility rate and duration of sperm were determined after exposure to cypermethrin. Reduced glutathione (GSH), glutathione peroxidase (GSH-Px), catalase (CAT) and malondialdehyde (MDA) in spermatozoa were analyzed for determination of oxidant and antioxidant balance. Our results indicated that spermatozoa motility and duration significantly decreased with exposure to cypermethrin. Additionally, activity of GSH-Px (P < 0.05) and MDA and GSH levels increased in a concentration-dependent manner while CAT activity decreased (P < 0.05). Consequently, the oxidant and antioxidant status and sperm quality were affected by quantitative changes and different concentrations of cypermethrin. (C) 2015 Elsevier Inc. All rights reserved.
AtıfWoS35OpenAlex38İncele - MakaleAçık Erişim2016Scopus SJRQ3Effects of Milk Thistle (Silybum marianum) Seed Supplementation to High-Calorie Basal Diets of Quails on Egg Production, Egg Quality Traits, Hatchability and Oxidative Stress Parameters
Erisir, Zeki; Iflazoglu Mutlu, Seda; Simsek, Ulku Gulcihan; Çiftçi, Mehmet; Azman, Mehmet Ali; Benzer, Fulya; Erisir, Mine
The purpose of this study was to examine the changes to occur in egg production, egg quality traits, hatchability, and oxidative stress parameters of laying quails fed with high calorie diets and effect of milk thistle (Silybum marianum) seed on these changes. A total of 75 45-day-old quails including 60 females and 15 males were used in the study. The quails were divided into 5 groups with three repetitions. 4 females and 1 male were used in each repetition. The groups of the study were arranged as following; control group (C) consuming corn-soybean based basal diet, oil group (SFO) in which 5% sun flower oil was added into basal diet, oil + milk thistle group (SFO+MT) in which 5% sun flower oil + 1% milk thistle seed was added into basal diet, syrup group (CS) in which 10% corn syrup was added into basal diet, and syrup + milk thistle group (CS+MT) in which 10% corn syrup + 1% milk thistle was added into basal diet. Total egg production was found to be significantly higher in SFO and SFO+MT groups of present study (P<0.05). Feed intake, total egg weight and feed conversion were found similar among the groups (P>0.05). The differences among the groups in albumen rate, yolk rate, dried shell rate and shape index were not statistically significant (P>0.05). The best yolk colour was obtained in SFO+MT and CS groups (P<0.01). There were no statistically differences among the groups in hatchability, hatchability of fertile and embrionic mortality (P>0.05), but the difference in examined fertility were statistically significant (P<0.05). Malonyl dialdehyde (MDA) values of blood, liver and heart tissues were similar among the groups of study (P>0.05). MDA level of kidney significantly increased in CS group, however addition of milk thistle seed into diet was reduced MDA level of kidney in CS+MT group (P<0.01). High calorie diets caused changes in antioxidant system in blood and tissues. Consequently, high-calorie basal diets especially addition of SFO improved only egg production and fertility rate. However feeding quails with corn syrup had significantly higher lipid peroxidation in kidney. Supplementation of milk thistle seed into CS group may protect kidney against free radical damage.
AtıfWoS8OpenAlex3İncele - MakaleAçık Erişim2015Scopus SJRQ2Rutin attenuates gentamicin-induced renal damage by reducing oxidative stress, inflammation, apoptosis, and autophagy in rats
Kandemir, Fatih Mehmet; Ozkaraca, Mustafa; Yildirim, Betul Apaydin; Hanedan, Basak; Kirbas, Akin; Kilic, Kubra; Aktas, Esra; Benzer, Fulya
Gentamicin is commonly used against gram-negative microorganisms. Its therapeutic use is mainly limited by nephrotoxicity. This study was aimed at evaluating the effect of rutin on oxidative stress, inflammation, apoptosis, and autophagy in gentamicin-induced nephrotoxicity in rats. The rats were treated with saline intraperitoneally (group I), 150 mg/kg of rutin orally (group II), 80 mg/kg of gentamicin intraperitoneally for 8 d (group III), or 150 mg/kg of rutin plus 80 mg/kg of gentamicin (group IV). The serum urea, creatinine, kidney malondialdehyde (MDA), and reduced glutathione (GSH) levels and superoxide dismutase (SOD), catalase (CAT), and glutathione peroxidase (GPx) activity and protein concentration were measured, and renal histopathology analysis and immunohistochemical staining were performed. Rutin pretreatment attenuated nephrotoxicity induced by gentamicin by reducing the urea, creatinine, and MDA levels and increasing the SOD, CAT, and GPx activity, and the GSH levels. The rutin also inhibited inducible nitric oxide synthase (iNOS), cleaved caspase-3 and light chain 3B (LC3B), as evidenced by immunohistochemical staining. The present study demonstrates that rutin exhibits antioxidant, anti-inflammatory, anti-apoptotic, and anti-autophagic effects and that it attenuates gentamicin-induced nephrotoxicity in rats.
AtıfWoS107OpenAlex123İncele - MakaleYalnız künye2015Scopus SJRQ4Arginase activity and total oxidant/antioxidant capacity in cows with lung cystic echinococcosis
Hanedan, Basak; Kirbas, Akin; Kandemir, F. Mehmet; Ozkaraca, Mustafa; Kilic, Kubra; Benzer, Fulya
The aim of this study was to investigate arginase activity, total oxidant capacity (TOC), total antioxidant capacity (TAC), and oxidative stress index (OSI) in cows with lung cystic echinococcosis (CE). The subjects were 20 cows with lung CE (parasitized group) and 20 healthy cows (control group). A significant increase in arginase activity, TOC, and OSI, and a significant decrease in TAC were found in the parasitized group compared with the control group (P < 0.001). Histopathological examination revealed a fibrous tissue reaction with inflammatory cell infiltration surrounding the CE in the lungs. The study revealed that increased arginase activity, TOC, and OSI levels and decreased TAC levels occurred in lung CE in cows. Increased arginase activity may be an important parameter for pulmonary fibrosis associated with lung CE in cows. Oxidant production due to chronic inflammation, as evidenced by the increased OSI levels, might contribute to persistent lung injury.
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