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- MakaleYalnız künye2022Scopus SJRQ2Sperm quality and oxidative stress in chub Squalius orientalis and Padanian barbel Barbus plebejus (Teleostei: Cyprinidae) after in vitro exposure to low doses of bisphenol A
Kutluyer, Filiz; Çakir Sahilli, Yeliz; Kocabaş, Mehmet; Aksu, Önder
In an aquatic environment, the presence of Bisphenol A (BPA) adversely affects reproduction, biology, behavior, gonads, and early larval development of fish due to being endocrine-disrupting compound. In addition, the detected concentration of BPA in water bodies is reported to be higher than 0.41 μg/L. As an alternative tool, sperm cells are used in toxicological assays for the reliable and practical assessment. For these reasons, we examined the effects of in vitro exposure of BPA on sperm quality of chub Squalius oriantalis and Padanian barbel Barbus plebejus. Spermatozoa were exposed to lower concentrations (0, 0.5, 1.25, 2.5, and 5 µg/L) of BPA for 2 h. The enzymatic activities [glutathione peroxidase (GSH-Px), catalase (CAT), and superoxide dismutase (SOD)] and lipid peroxidation (MDA) were evaluated in spermatozoa. The results demonstrated that BPA exposure significantly decreased activities of SOD and GSH-Px but increased CAT activity and lipid peroxidation (MDA). Compared to control, the percentage and duration of sperm motility significantly decreased. Overall, spermatozoa clearly showed the sensitivity to lower concentrations of BPA.
AtıfScopus16WoS17OpenAlex14
İncele - MakaleYalnız künye2021Scopus SJRQ2Cellular responses of spirlin Alburnoides bipunctatus spermatozoa exposed to Bisphenol A: Biochemical response and sperm quality alterations evaluation
Kocabaş, Mehmet; Kutluyer, Filiz; Çakir Sahilli, Yeliz; Aksu, Önder
Plastics are one of the global environmental problems owing to their highest production. Bisphenol A (BPA) is used in the manufacturing of plastics including polycarbonate, polyvinyl chloride and resins. In particular, aquatic ecosystems are adversely affected by the occurrence of micropollutants. In addition, BPA as a typical endocrine-disrupting chemical (EDC) seriously influences androgenic activity and reproduction of aquatic organisms, particularly fish as non-target organism. In toxicological assays, sperm cells can be used as a potential tool for reliable and practical assessment. For these reasons, sperm cells were exposed to lower concentrations (0 µg/L, 0.5 µg/L, 1.25 µg/L, 2.5 µg/L and 5 µg/L) of BPA and the study focused on BPA-induced oxidative stress. The enzymatic [glutathione peroxidase (GSH-Px), catalase (CAT) and superoxide dismutase (SOD)] activities and lipid peroxidation (MDA) were analyzed in spermatozoa. The study findings showed that the activities of SOD and GSH-Px were significantly decreased by BPA but lipid peroxidation (MDA) and CAT acticity increased. Notably, the percentage and duration of motile sperm cells decreased. Collectively, spermatozoa clearly showed the sensitivity even at extremely low concentrations. This is first report to assess the impact of BPA on sperm cells of spirlin Alburnoides bipunctatus.
AtıfScopus6WoS6OpenAlex5İncele - MakaleYalnız künye2020Scopus SJRQ2Investigation of antioxidant responses in Gammarus pulex exposed to Bisphenol A
Tatar, Şule; Türkmenoğlu, Yener
One of the most important environmental problems in the world is micro-pollutants. The aim of this study was to investigate the antioxidant responses of Gammarus pulex to Bisphenol A (BPA), an endocrine-disrupting agent. For this purpose, sublethal concentrations of BPA were applied to G. pulex and biochemical responses were studied. Enzymatic antioxidants superoxide dismutase (SOD) and catalase (CAT) activities and nonenzymatic antioxidants glutathione (GSH) and thiobarbituric acid reagents (TBARS) levels in G. pulex were determined in four different groups during 24 and 96 h. Biochemical biomarkers were measured using commercial kits in a microplate reader. When we compared with control, SOD enzyme activity increased in all groups during both administration periods and CAT enzyme activity decreased in all groups. GSH and TBARS levels were increased after 24 and 96 h of application periods in all groups when compared with control. For changes in SOD and CAT activities and GSH, TBARS levels have been determined to be useful as biomarkers against BPA in G. pulex tissues. It has also been proven that G. pulex is an effective bioindicator that shows BPA pollution in water. The different results of biochemical biomarkers can be evaluated as a marker of possible metabolic processes, and the biochemical response of G. pulex can reveal to some extent the environmental consequences of BPA pollution resulting from industrial waters.
AtıfScopus5WoS3OpenAlex7İncele - MakaleAçık Erişim2016Scopus SJRQ2Adsorption of bisphenol A from aqueous solutions by Pleurotus eryngii immobilized on Amberlite XAD-4 using as a new adsorbent
Yonten, Vahap; Ince, Muharrem; Tanyol, Mehtap; Yildirim, Numan
The adsorption of bisphenol A (BPA) in a fixed-bed column was evaluated in this work. Fungus Pleurotus eryngii isolated from Tunceli (Turkey) immobilized on XAD-4 resin was used as an adsorbent for decontamination of BPA from aqueous solutions. The extent of BPA removal capacity was evaluated as a function of parameters such as pH, volume of the sample, and column bed height. Adsorption isotherms of BPA on immobilized P. eryngii were determined and correlated with common isotherm equations such as Langmuir and Freundlich models. The mechanism of adsorption of BPA was analyzed preadsorption (XAD-4+fungus) and postadsorption (XAD-4+fungus+BPA) using fourier transform infrared spectroscopy. The results show that adsorption ability of BPA by immobilized P. eryngii is possible and this system yields the best degree of BPA removal (90%) at pH 11 for an initial load of 120mgL(-1). Therefore, this material can be used as a very effective adsorbent for BPA decontamination in aqueous solutions.
AtıfWoS17OpenAlex21İncele